Mountain glaciers are an important component of the global hydrological cycle. Existing research about glacier changes in the Altai focused on limited regions. Study about recent glacier changes in the entire Altai Mountains is still lacking. We presented a consistent method for identifying glacier margins. The two new glacier inventories in 2000 and 2020 were derived from Landsat satellite imagery. Glacier surface elevation change and mass balance were obtained by comparing the 2000 Shuttle Radar Topography Mission (SRTM) and 2020 Digital Elevation Models (DEMs) generated from Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) images. The spatial pattern of glacier changes was discussed in conjunction with climate trends. We mapped a total area of 1,096.06 ± 53.32 km2around 2020, which amounts to 1,927 glaciers in the Altai Mountains. That was 12.02 ± 3.01% (or 0.60 ± 0.15%·a−1) less than the 1,245.75 ± 58.52 km2around 2000. The geodetic mass balance of the monitoring glaciers in the Aktru basin for the period 2000–2011 was used to validate the geodetic survey. The average geodetic mass balance of -0.32 ± 0.09 m w. e.·a−1on monitoring glaciers was slightly exaggerated than the observed mass balance of -0.26 m w. e.·a−1, but it was proved that the geodetic mass balance could reflect glacier changes in the Altai Mountains. An average mass loss of 14.55 ± 1.32 m w. e. (or 0.74 ± 0.07 m w. e.·a−1) was found during 2000–2020 in the Altai Mountains. Although the glacier area changes and mass balance were characterized by spatial heterogeneity, the glaciers in the Altai had experienced an accelerated shrinkage from 2000 to 2020 compared to the 20th century. The rising temperature is the foremost reason for glacier area shrinkage and mass loss according to the Climatic Research Unit (CRU) reanalysis data.
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