The significance for inelastic design of moment-inelastic rotation behavior with respect to interior pier sections of steel girder bridges is experimentally investigated. Under center span loading conditions, 12 welded, built-up, simply supported beams with various slenderness ratios of the flange and web plates are tested. In this test, lengths and locations for partial longitudinal stiffeners on the web plates are varied, and the results are then compared with the inelastic deformation capacity of beams without longitudinally stiffened web plates. The results are also compared with the inelastic design code in AASHTO LRFD bridge design specifications. It is concluded that (1) the ultimate strength of stiffened beams is governed by the local buckling at the compression flange of the far end from the loading point due to the presence of a partial longitudinal stiffener; and (2) the inelastic rotation capacity and ultimate strength of beam with a stiffened web plate are remarkably improved. The optimum length and location of stiffeners on the plates are given.