Simulated Spaceflight Research Articles

The Integration of AI and Metaverse in Education: A Systematic Literature Review

The use of the metaverse in educational environments has grown significantly in recent years, particularly following the shift of major tech companies towards virtual worlds and immersive technologies. Virtual reality and augmented reality technologies are employed to construct immersive learning environments. The metaverse is generally understood as a vast digital ecosystem or virtual space, facilitating the transition of individuals from physical to virtual environments, and is applicable to educational domains where practical experiments are challenging or fraught with risks, such as space exploration, chemical experimentation, and flight simulation training. In addition, the integration of artificial intelligence with the metaverse within educational contexts has significantly enriched the learning environment, giving rise to AI-driven teaching systems tailored to each student’s individual pace and learning modalities. As a result, a number of research articles have been conducted to explore the applications of the metaverse and artificial intelligence in education. This paper provides a systematic literature review following the PRISMA methodology to analyze and investigate the significance and impact of the metaverse in education, with a specific focus on the integration of AI with the metaverse. We address inquiries regarding the applications, challenges, academic disciplines, and effects of integrating AI and the metaverse in education that have not yet been explored in most research articles. Additionally, we study the AI techniques used in the metaverse in education and their roles. The review affirms that the integration of the metaverse in education, with the utilization of AI applications, will enrich education by improving students’ understanding and comprehension across diverse academic disciplines.

Systematic screening of 42 vancomycin-resistant Enterococcus faecium strains for resistance, biofilm, and desiccation in simulated microgravity

Vancomycin-resistant Enterococcus faecium (VRE) presents significant challenges in healthcare, particularly for hospitalized and immunocompromised patients, including astronauts with dysregulated immune function. We investigated 42 clinical E. faecium isolates in simulated microgravity (sim. µg) using a 2-D Clinostat, with standard gravity conditions (1 g) as a control. Isolates were tested against 22 antibiotics and characterized for biofilm formation and desiccation tolerance. Results showed varied responses in minimum inhibitory concentration (MIC) values for seven antibiotics after sim. µg exposure. Additionally, 55% of isolates showed a trend of increased biofilm production, and 59% improved desiccation tolerance. This investigation provides initial insights into E. faecium’s changes in response to simulated spaceflight, revealing shifts in antibiotic resistance, biofilm formation, and desiccation tolerance. The observed adaptability emphasizes the need to further understand VRE’s resilience to microgravity, which is crucial for preventing infections and ensuring crew health on future long-duration space missions.

Analysis of the development of the structure and dynamics of relationships and bonds in the crew of a short-term simulated space flight to the Moon

Analysis of the development of the structure and dynamics of relationships and bonds in the crew of a short-term simulated space flight to the Moon

Self-Generated Lower Body Negative Pressure Exercise: A Low Power Countermeasure for Acute Space Missions.

Microgravity in spaceflight produces headward fluid shifts which probably contribute to Spaceflight-Associated Neuro-Ocular Syndrome (SANS). Developing new methods to mitigate these shifts is crucial for preventing SANS. One possible strategy is the use of self-generated lower body negative pressure (LBNP). This study evaluates biological or physiological effects induced by bed rest to simulate adaptations to microgravity. Participants were tested during powered LBNP and dynamic self-generated (SELF) LBNP at 25 mmHg for 15 min. The results were compared to the physiologic responses observed in seated upright and supine positions without LBNP, which served as controls for normal gravitational effects on fluid dynamics. Eleven participants' (five male, six female) heart rates, blood pressures, and cross-sectional areas (CSA) of left and right internal jugular veins (IJV) were monitored. Self-generated LBNP, which requires mild to moderate physical activity, significantly elevated heart rate and blood pressure (p < 0.01). Self-generated LBNP also significantly reduced right IJV CSA compared to supine position (p = 0.005), though changes on the left side were not significant (p = 0.365). While the effects of SELF and traditional LBNP on IJV CSA were largely similar, traditional LBNP significantly reduced IJV CSA on both sides. Given its low mass, volume, and power requirements, SELF LBNP is a promising countermeasure against SANS. Results from this study warrant longer-term studies of SELF LBNP under simulated spaceflight conditions.

Modelling large scale artery haemodynamics from the heart to the eye in response to simulated microgravity

We investigated variations in haemodynamics in response to simulated microgravity across a semi-subject-specific three-dimensional (3D) continuous arterial network connecting the heart to the eye using computational fluid dynamics (CFD) simulations. Using this model we simulated pulsatile blood flow in an upright Earth gravity case and a simulated microgravity case. Under simulated microgravity, regional time-averaged wall shear stress (TAWSS) increased and oscillatory shear index (OSI) decreased in upper body arteries, whilst the opposite was observed in the lower body. Between cases, uniform changes in TAWSS and OSI were found in the retina across diameters. This work demonstrates that 3D CFD simulations can be performed across continuously connected networks of small and large arteries. Simulated results exhibited similarities to low dimensional spaceflight simulations and measured data—specifically that blood flow and shear stress decrease towards the lower limbs and increase towards the cerebrovasculature and eyes in response to simulated microgravity, relative to an upright position in Earth gravity.

ПСИХОЛОГИЧЕСКИЕ ОСОБЕННОСТИ АДАПТАЦИИ ОРГАНИЗМА ЗДОРОВОГО ЧЕЛОВЕКА К МОДЕЛИРУЕМЫМ ФАКТОРАМ КОСМИЧЕСКОГО ПОЛЕТА В ЭКСПЕРИМЕНТЕ С 21-СУТОЧНОЙ «СУХОЙ» ИММЕРСИЕЙ

The 21-d dry immersion study with participation of 8 male volunteers at the age of 25 to 33 years included investigations of the psychological aspects of adaptation to simulated spaceflight factors. Psychological testing 14 days prior to DI and on day 21 of the study was performed using the multilevel adaptivity inventory (А.G. Maklakova and S.V. Chermianina), the inventory of mental activity, interests, affective tone, strain and comfort (L.A. Kurgansky and Т.А. Nemchina), well-being in extreme conditions inventory (А. Volkova and N.E. Vodopianova) and situation-adaptable subjective vitality scale (R. Rayan and S. Frederick, apecialized by L.A. Aleksandrova). Participants in the study adapted successfully to the conditions as could be deduced from stability of their personal adaptation potential. At the end of the study the participants were noted to be more neurotic, demonstrated symptoms of autonomic regulation imbalance, disorders in the sleep wakefulness cycles, psychological discomfort and reduction of the energy potential. These changes witnessed to a moderate stress reaction to the DI conditions.

Sleep Disruption, Fatigue, and Altered Neurobehavioral Performance Among Flight Controllers During Spaceflight Operations

In spaceflight operations, flight controllers manage technical aspects of spaceflight control and spacecraft systems. The flight control team is a group subjected to shift work. Acute and chronic exposure to shift work has been associated with circadian misalignment, sleep impairment, and a negative impact on cognitive performance. This study aims to review the effects of shift work on sleep, circadian rhythms, mood, and cognitive performance of flight controllers during real and simulated spaceflight operations. Shift work during low-Earth orbit spaceflight missions is associated with a reduction in alertness, motivation, processing speed, and working memory performance efficiency. Flight controllers also report excessive insomnia and insufficient total sleep time. The development of shift work sleep disorder may be present in up to 40% of workers, especially among night and evening shift workers. Mars operations and Mars-simulated missions are associated with an impairment of visual-motor performance, working memory efficiency, and reaction time. Between 50%–87% of controllers can synchronize their circadian rhythm to a Mars day (24.65 hours), although this adaptation is not reflected in improved neurobehavioral performance.

Simulated spaceflight-induced cardiac remodeling is modulated by gut microbial-derived trimethylamine N-oxide

Spaceflight is physically demanding and can negatively affect astronauts' health. It has been shown that the human gut microbiota and cardiac function are affected by spaceflight and simulated spaceflight. This study investigated the effects of the gut microbiota on simulated spaceflight-induced cardiac remodeling using 10° of head-down bed rest (HDBR) in rhesus macaques and 30° of hindlimb unloading (HU) in mice. The gut microbiota, fecal metabolites, and cardiac remodeling were markedly affected by HDBR in macaques and HU in mice, cardiac remodeling in control mice was affected by the gut microbiota of HU mice and that of HU mice was protected by the gut microbiota of control mice, and there was a correlation between cardiac remodeling and the gut microbial-derived metabolite trimethylamine N-oxide. These findings suggest that spaceflight can affect cardiac remodeling by modulating the gut microbiota and fecal metabolites.

Application of a Psychosocial Approach to the Identification and Strengthening of Adaptation Mechanisms of Humans and a Small Social Group during the Isolation Experiment “SIRIUS 2017–2023”

TOPIC: The task of the 21st century is the implementation of manned flights in Earth’s orbit with the view to building orbital and planetary bases. This requires addressing the impacts on people and small social groups in terms of psychological, psychosocial, physiological and health. The author presents her own comprehensive research and intervention approach to exploring and supporting the operation of the space crew in the four-month isolation period of “SIRIUS-18/19”, which can be used in the future for manned flights into deep space. GOAL: The main goal is to present three main areas, within the implementation of social research, designed to analyze the operation of the crew in a simulated space flight: 1. WORKING CONDITIONS, WORKING ENVIRONMENT AND SOCIAL ATMOSPHERE; 2. the STRUCTURE AND DYNAMICS OF RELATIONSHIPS and TIES; 3. a set of other specific areas. The key outputs of the comprehensive analysis of the “SIRIUS-19” crew operations concerning the level of satisfaction with the working environment and conditions, the structure and dynamics of relationships and other specific areas are presented. The suitability of the implementation of intervention activities for isolated crews is pointed out. The purpose is to contribute to the preparation of human crews for manned flights in deep space and to reduce the risks of damage to human biopsychosocial health. METHODS: For a comprehensive analysis, a set of the author’s own questionnaire methods, verified over 25 years in the normal and extremely demanding conditions of specific professions, was used. The diagnostic and intervention method sociomapping, based on fuzzy theory and the mathematical modeling of outputs, was used for the analysis of the structure and dynamics of relationships as it is a technique suitable for the analysis of nonlinear dynamical systems. The methodology enabled the author to obtain a comprehensive view of the experimental situation from a psychosocial and sociological point of view. RESULTS: The model of the author’s analytical approach confirmed the legitimacy of its implementation in the case of isolation experiments. A comprehensive analysis of the “SIRIUS-18/19” crew’s work environment yielded outputs from the 10 main and 48 sub-areas analyzed. The analysis of the six-member, gender-mixed, multicultural crew in the area of structure and dynamics of relationships focused on 35 areas; a total of 344 sociomaps were created. The files were analyzed qualitatively and quantitatively using control diagrams. CONCLUSIONS: Outputs have the potential to be used in other isolation experiments as sociotechnical measures for project organizers and as verification of the need to introduce work with the crew in the form of development workshops using the sociomapping method.

Zwitterionic Copolymers for Anti-Scaling Applications in Simulated Spaceflight Wastewater Scenarios.

Water reclamation in spaceflight applications, such as those encountered on the International Space Station (ISS), requires complex engineering solutions to ensure maximum water recovery. Current vapor compression distillation (VCD) technologies are effective but produce highly concentrated brines and often cause scaling within a separation system. This work evaluates initial steps toward integrating pervaporation, a membrane separation process, as a brine management strategy for ISS wastewaters. Pervaporation performs separations driven by a chemical potential difference across the membrane created by either a sweep gas or a vacuum pull. Pervaporation membranes, as with most membrane processes, can be subject to scaling. Therefore, this work studies the anti-scaling properties of zwitterions (polymeric molecules with covalently tethered positive and negative ions) coated onto sulfonated pentablock terpolymer block polymer (Nexar) pervaporation membrane surfaces. We report a method for applying zwitterions to the surface of pervaporation membranes and the effect on performance parameters such as flux and scaling resistance. Membranes with zwitterions had up to 53% reduction in permeance but reduced scaling. The highest amount of scaling occurred in the samples exposed to calcium chloride, and uncoated membranes had weight percent increases as high as 1617 ± 241%, whereas zwitterion-coated membranes experienced only about 317 ± 87% weight increase in the presence of the same scalant.

Remains Containment Considerations for Death in Low-Earth Orbit

BACKGROUND: Maintenance and disposition of decedent remains during spaceflight require the isolation of biohazardous products of decomposition in microgravity and in the absence of refrigeration. Containment and isolation options would preferably offer sufficient time to enable crew and ground support teams to determine appropriate disposition of remains and even potentially return remains to the Earth. The pilot study described herein undertook an effort to develop a postmortem containment unit for the isolation and maintenance of decedent remains in a microgravity environment.METHODS: Commercial off-the-shelf containment units were modified to meet the needs of a microgravity spaceflight environment and to offer the best likelihood of successful containment and management of remains. A subsequent evaluation of modified containment unit performance was undertaken utilizing human cadavers, with measurement and analysis of volatile off-gassing over time followed by impact testing of the units containing cadaverous remains in a simulated spaceflight vehicle seat.RESULTS: Modifications were implemented without significant negative design impact. Failure was observed in one modified unit after 9 d and attributed to improper filter application. The remaining unit successfully contained remains beyond the intended endpoint of the study.DISCUSSION: These pilot efforts offer important insight into the development of effective postmortem containment options for future spaceflight. Further study is needed to ensure repeatability of the findings and to further characterize the failure modes of the modified units evaluated, the impact of microgravity conditions, and the identification of additional modifications that would improve remains disposition.Houser T, Lindgren KN, Mazuchowski EL II, Barratt MR, Haines DC, Jayakody M, Blue RS, Bytheway JA, Stepaniak PC. Remains containment considerations for death in low-Earth orbit. Aerosp Med Hum Perform. 2023; 94(5):368-376.

Ultrasound assessments of organs and blood vessels before and after 40days isolation in a cavern (deep time experiment 2021).

Introduction: Spaceflight simulation studies like confinement in small volume habitat with limited physical activity have reported even after 60days an abnormal arterial wall adaptation with increase thickness or stiffness. The purpose of the current study was to determine the effects on blood vessel and organ structure of 40days of isolation in a huge habitat with intensive physical activity. Method: Data were collected from 14 individuals (7 male) who isolated in a cavern for 40-days while performing normal daily activities without time references. Ultrasound assessments were performed pre- and post-isolation using a teleoperated system with eight different acoustic windows to obtain 19 measurements on 12 different organ/vascular structures which included the common carotid artery, femoral artery, tibial artery, jugular vein, portal vein, bile duct, kidney, pancreas, abdominal aorta, cervical and lumbar vertebral distance, and Achilles tendon. Results: Common carotid artery measures, including the intima media thickness, stiffness index, and the index of reflectivity measured from the radiofrequency signal, were not changed with isolation. Similarly, no differences were found for femoral artery measurements or measurements of any of the other organs/vessels assessed. There were no sex differences for any of the assessments. Discussion: Results from this study indicate a lack of physiological effects of 40-days of isolation in a cavern, contrary to what observed in previous 60days confinement. This suggests a potential protective effect of sustained physical activity, or reduced environmental stress inside the huge volume of the confined facility.

Loose coupling in future distributed space safety simulations

Distributed simulations play an important role in complex space safety related examinations. Realizing an associated system-of-systems solution with as less software adaptation development effort as possible represents a key challenge in this context. The paper presents a microservice-based approach, considering possibilities of the new Simulation Interoperability Standards Organization (SISO) standard Space Reference Federate Object Model. Different High-Level Architecture (HLA) compliant solutions are conversed, ranging from usage of the HLA evolved standard's web service application programming interface (API) over the implementation of a tightly coupled bridging federate to the exploitation of the future HLA (HLA 4) version's standardized federate protocol. A human-in-the-loop space flight simulator concept use case is presented and discussed.

Effects of Simulated Long Duration Spaceflight on Erectile Function

The goal of NASA along with other space agencies in the world is to send manned missions to the moon in the coming years, and eventually to Mars. The long‐term health risks of spaceflight have been attributed primarily to cosmic radiation and microgravity and the adverse effects as seen in the astronauts and model animals include DNA damage, mitochondrial dysregulation, oxidative damage, epigenetic changes, telomere length changes and microbiome shifts all of which affect the cardiovascular system. However, little is known about the effect of spaceflight on erectile function which is an important quality of life aspect for men. The aim of our study was to investigate the effect of simulated long duration space flight on erectile function. We hypothesized that both hindlimb unloading (HLU) and radiation will adversely affect erectile function. 86 adult male Sprague‐Dawley rats were used for these studies. Half the animals underwent 4 weeks of HLU, and all animals were divided into 3 groups: those that received sham radiation, 0.75 Gy, and 1.5 Gy galactic cosmic radiation, respectively at the Ground‐based GCR simulator at the NASA Space Radiation laboratory. The animals were then monitored for 6‐9 months before they were sacrificed, following which Corpus cavernosum (CC) and the distal internal pudendal arteries (dIPA) were harvested and used for ex vivo functional assessment. Tissue and wire myography studies were performed to assess adrenergic and endothelium‐dependent vasoconstrictions using phenylephrine (PE), norepinephrine, and endothelin. This was done by measuring the cumulative dose‐responses to the agonists in both the CC and dIPA. Endothelium‐dependent and ‐independent relaxations were also assessed using acetylcholine and diethylamine nononate. Non‐adrenergic non‐cholinergic (NANC) transmission was assessed using electrical stimulation (Estim); 30V electric pulses were delivered to the tissues with frequency ranging from 1‐32Hz following the administration of atropine and guanethidine and pre‐constriction with PE. Finally, Estim was done without atropine and guanethidine to assess the parasympathetic mediated relaxation in the tissues. The effects of HLU and radiation on the contractile and relaxation responses were assessed by two‐way repeated measures ANOVA. HLU and radiation significantly reduced both endothelium‐dependent and ‐independent vasodilatation in the dIPA and CC. Also, HLU and radiation significantly reduced the adrenergic constriction of CC in response to PE and norepinephrine. However, neither HLU nor radiation influenced the endothelium‐dependent vasoconstriction in the dIPA. Finally, radiation reduced the relaxation in both CC and dIPA while HLU increased the dIPA relaxation following Estim for both the parasympathetic mediated and NANC mediated responses. These results therefore showed the effects of HLU and radiation on erectile function, with exposure to cosmic radiation being primarily responsible for the detrimental effects. Further research needs to be done on this subject to show more mechanistically how spaceflight can affect erectile function and ways to combat the problem to ensure that astronauts can return to their normal sexual lives after long trips to space.

Zwitterionic Copolymers for Anti-Scaling Applications in Simulated Spaceflight Wastewater Scenarios

Zwitterionic Copolymers for Anti-Scaling Applications in Simulated Spaceflight Wastewater Scenarios

Exploring Personality and Stress during Communication Delays in Simulated Spaceflight Missions

Exploring Personality and Stress during Communication Delays in Simulated Spaceflight Missions

Tend-and-befriend behaviour during spaceflight simulation

Stress under extreme conditions can promote not only negative reactions but also prosocial behaviour or provision of protection and social support. This study presents research aimed at detecting the ‘tend-and-befriend’ stress response among crewmembers of the SIRIUS-19 international spaceflight simulation (120 days, 3 men, 3 women). Emotional energy proved to be a good indicator of individual capability to emotionally invest in relationships with crewmates. To measure emotional energy, we applied a subscale of Shirom-Melamed Vigor Measure. Personal Self Perception and Attitudes computerised test and sociometry were used to assess cohesion and psychological closeness in the crew. The results show a high level of emotional energy invested by the crew in their crewmates. Crew cohesion, assessed on the basis of reciprocal sociometric choices, and psychological closeness, measured as perceived similarity, both tended to increase during the isolation experiment. In a mixed crew, both men and women showed high levels of emotional energy and social support. It seems therefore that in a cohesive crew, emotional relationships – expressed in increased psychological closeness, emotional energy, and the tend-and-befriend behaviour (provision of mutual protection and assistance) – represent the dominant prosocial response to the challenge of social isolation. This line of research deserves further development in the context of simulated space flights and would profit from further work on instruments aimed at a deeper analysis of emotional relationships in small isolated groups.

Developing, Implementing, and Applying Novel Techniques During Systematic Reviews of Primary Space Medicine Data

BACKGROUND: The Aerospace Medicine Systematic Review Group was set up in 2016 to facilitate high quality and transparent synthesis of primary data to enable evidence-based practice. The group identified many research methods specific to space medicine that need consideration for systematic review methods. The group has developed space medicine specific methods to address this and trialed usage of these methods across seven published systematic reviews. This paper outlines evolution of space medicine synthesis methods and discussion of their initial application.METHODS: Space medicine systematic review guidance has been developed for protocol planning, quantitative and qualitative synthesis, sourcing gray data, and assessing quality and transferability of space medicine human spaceflight simulation study environments.RESULTS: Decision algorithms for guidance and tool usage were created based on usage. Six reviews used quantitative methods in which no meta-analyses were possible due to lack of controlled trials or reporting issues. All reviews scored the quality and transferability of space simulation environments. One review was qualitative. Several research gaps were identified.CONCLUSION: Successful use of the developed methods demonstrates usability and initial validity. The current space medicine evidence base resulting in no meta-analyses being possible shows the need for standardized guidance on how to synthesize data in this field. It also provides evidence to call for increasing use of controlled trials, standardizing outcome measures, and improving minimum reporting standards. Space medicine is a unique field of medical research that requires specific systematic review methods.Winnard A, Caplan N, Bruce-Martin C, Swain P, Velho R, Meroni R, Wotring V, Damann V, Weber T, Evetts S, Laws J. Developing, implementing, and applying novel techniques during systematic reviews of primary space medicine data. Aerosp Med Hum Perform. 2021; 92(8):681688.

Defining the resilience of the human salivary microbiota by a 520-day longitudinal study in a confined environment: the Mars500 mission

BackgroundThe human microbiota plays several roles in health and disease but is often difficult to determine which part is in intimate relationships with the host vs. the occasional presence. During the Mars500 mission, six crewmembers lived completely isolated from the outer world for 520 days following standardized diet regimes. The mission constitutes the first spaceflight simulation to Mars and was a unique experiment to determine, in a longitudinal study design, the composition and importance of the resident vs. a more variable microbiota—the fraction of the human microbiota that changes in time and according to environmental conditions—in humans.MethodsHere, we report the characterization of the salivary microbiota from 88 samples taken during and after Mars500 mission for a total of 720 days. Amplicon sequencing of the V3–V4 regions of 16S rRNA gene was performed, and results were analyzed monitoring the diversity of the microbiota while evaluating the effect of the three main variables present in the experimental system: time, diet, and individuality of each subject.ResultsResults showed statistically significant effects for either time, diet, and individuality of each subject. The main contribution came from the individuality of each subject, emphasizing salivary microbiota-personalized features, and an individual-based resilience of the microbiota.ConclusionsThe uniqueness of Mars500 mission, allowed to dampen the effect of environmental variables on salivary microbiota, highlighting its pronounced personalization even after sharing the same physical space for more than a year.DRuCc4_oUAP4YMwxG5Lf3TVideo abstract

Detection of Genes in Arabidopsis thaliana L. Responding to DNA Damage from Radiation and Other Stressors in Spaceflight

Ionizing radiation present in extraterrestrial environment is an important factor that affects plants grown in spaceflight. Pearson correlation-based gene regulatory network inferencing from transcriptional responses of the plant Arabidopsis thaliana L. grown in real and simulated spaceflight conditions acquired by GeneLab, followed by topological and spectral analysis of the networks is performed. Gene regulatory subnetworks are extracted for DNA damage response processes. Analysis of radiation-induced ATR/ATM protein–protein interactions in Arabidopsis reveals interaction profile similarities under low radiation doses suggesting novel mechanisms of DNA damage response involving non-radiation-induced genes regulating other stress responses in spaceflight. The Jaccard similarity index shows that the genes AT2G31320, AT4G21070, AT2G46610, and AT3G27060 perform similar functions under low doses of radiation. The incremental association Markov blanket method reveals non-radiation-induced genes linking DNA damage response to root growth and plant development. Eighteen radiation-induced genes and sixteen non-radiation-induced gene players have been identified from the ATR/ATM protein interaction complexes involved in heat, salt, water, osmotic stress responses, and plant organogenesis. Network analysis and logistic regression ranking detected AT3G27060, AT1G07500, AT5G66140, and AT3G21280 as key gene players involved in DNA repair processes. High atomic weight, high energy, and gamma photon radiation result in higher intensity of DNA damage response in the plant resulting in elevated values for several network measures such as spectral gap and girth. Nineteen flavonoid and carotenoid pigment activations involved in pigment biosynthesis processes are identified in low radiation dose total light spaceflight environment but are not found to have significant regulations under very high radiation dose environment.