Oil-paper as a reliable insulation system is widely used in power transformers and cables. The dielectric properties of oil-paper insulation play an important role in the reliable operation of power equipment. However, the formation and dynamics of space charge can affect the performance of insulation material. In this paper, space charge in oil-paper insulation system has been investigated using the pulsed electroacoustic (PEA) technique. A series of measurements were carried out when the insulation system was subjected to different applied voltages at different temperatures. Charge behavior in the insulation system has been analyzed and the influence of temperature on charge dynamics was discussed. The test results show that homocharge injection takes place under all the test conditions, the applied dc voltage mainly effect the amount of space charge, while the temperature has greater influence on the distribution and mobility of space charge inside oil-paper samples.