The genetic variability of soybean grains allows its grow in different regions, where the adaptability of the genotypes to environments, and sowing times can affect the isoflavone profile and post-harvest conservation. Thus, the objective was to evaluate the genotype effects, sowing date, and storage time on technological properties and isoflavone profile of soybean. For this, it was used the soybean genotypes BMX LANÇA IPRO, NA 5909 RG, BMX ATIVA RR, and BMX GARRA IPRO, cultivated in standard (October 25th) and a late sowing date (November 15th). Analyzes performed in the present study were acidity, lipase activity, soluble protein, germination, electric conductivity, soybean defects, colorimetric profile, and isoflavone profile. Higher levels of isoflavones were found in the BMX GARRA IPRO genotype. The genotype BMX ATIVA RR when cultivated in late sowing date showed a reduced isoflavones contents. The NA 5909 RG genotype showed lower adaptability to the conditions of the environment where it was cultivated and higher susceptibility to prolonged storage, showing higher values of acidity, lipase activity, and lower values of soluble protein and germination. The BMX LANÇA IPRO genotype showed higher adaptability to the conditions of the environment where it was cultivated and to prolonged storage, showing higher values of soluble protein, and germination, and lower values of electrical conductivity, and soybean defects, proving the effect of genotype on soybean growing conditions. A reduction in soluble protein, germination, b*-value, and L*-value and an increase in acidity, lipase activity, electrical conductivity, and fermented grains during storage were observed.