Southern Poland Research Articles

Accumulation ability of trace metals by silver birch leaves in areas contaminated by Zn–Pb ore processing: Effects of excessive trace metal accumulation on specialized metabolism

The study was carried out in the most industrialised region of southern Poland. Four areas were selected for the study: the vicinity of the Miasteczko Śląskie Zn–Pb-works (ZW; with three transects selected: A, B, and C), and the metalliferous waste heaps (H1, H2, H3). Samples were taken from the surface soil layer, while only mature silver birch (Betula pendula Roth) trees (3–4 m high) growing spontaneously in the study area were selected for the collection of leaves. The analysis of metals including trace metals (TMs; Ag, Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Ni, Pb, Tl, and Zn) in the soil and leaves, as well as the contents of specialized leaf compounds belonging to polyphenols were performed. In general, the B. pendula trees in transects A and B located within 500 m of the furnaces exhibited 3–9 times higher content of TMs in their leaves compared to the plants located further away from the Zn smelter. In contrast, the quantity of TM in trees from transect C was independent of distance. The accumulation of TMs in the leaves was significantly lower in the metalliferous waste heap sites compared to the ZW area. It was hypothesised that the accumulation of TMs in B. pendula leaves would depend on the location and distance from the polluting source. It was demonstrated that TMs content in soil affects the accumulation of phenolic acids and flavonoids.

Relationships between pesticides, polychlorinated biphenyls, blood parameters and oxidative stress of white stork Ciconia ciconia chicks in Poland

The white stork Ciconia ciconia is a bird species located at the top of the trophic pyramid in grassland and wetland ecosystems. This charismatic species is susceptible to pesticides and their environmental residues. In 2016, we collected blood samples from 114 white stork chicks across Western and Southern Poland. Chicks were sexed by molecular analysis and aged by development pattern. We studied the relationship between the concentration of pesticides (beta-HCH, heptachlor, aldrin, endrin, 4.4′-DDD, 4.4′-DDE and 4.4′-DDT) and of PCB in the chicks’ blood with blood morphology and biochemistry parameters in the blood. The mean (± SD) values of concentrations of above detection level pesticides were: for (1) beta-HCH 4.139 ± 19.205; (2) 4.4′-DDE 9.254 ± 91.491 and additionally (3) PCB 16.135 ± 44.777 ppb. We found negative relationships between beta-HCH and oxidative stress enzyme activity in the blood, between beta-HCH and leukocyte concentration and between 4.4′-DDE and catalase activity. We also found a positive relationship between the concentration of pesticides in blood and the age of chicks. Interestingly, we found a higher concentration of PCB in the blood of male stork chicks than in female stork chicks. We provide more evidence that the presence of pesticides in the environment can be a strong stress factor, shaping the health status of birds.

Job satisfaction and social identification among paramedics in southern Poland.

Job satisfaction, based on professional and non-professional factors and individual characteristics of employees, is an important element influencing both the quality of care provided and employee turnover. The study included 137 paramedics employed in field teams and hospital emergency departments. The Job Satisfaction Scale (SSP), the Minnesota Job Satisfaction Questionnaire (MSQ), the Utrecht Work Engagement Scale (UWES), and the Three Dimensional Strength of Group Identification Scale (TSIG) were used to collect the data. The average job satisfaction score measured with SSP in the studied group of paramedics was 24.50 and the average job satisfaction score measured with MSQ was 74.16. The average value of the group identification in the study sample was 61.15. Of the three subscales, the highest scores were obtained in the affect toward the group subscale -22.44, and the lowest in the cognitive centrality subscale -18.78. The analysis showed that job satisfaction positively correlated with social identification (r = 0.43) and the ingroup ties (r = 0.43), cognitive centrality (r = 0.34) and ingroup affect (r = 0.37). The studied group of paramedics showed moderate job satisfaction (measured with SSP) and work engagement, with a simultaneous high level of job satisfaction (measured with MSQ) and social identification with the professional group. Social identification of studied paramedics varied depending on gender. Women showed higher levels of cognitive centrality, which might mean that they might have had greater need to categorize themselves as paramedics.

Exploring the Role of Shrubs in Modulating Heavy Metal Accumulation in Forest Soils in Single-Species Pine Stands

Heavy metals accumulate and bind strongly in soil, making them difficult to eliminate. The objective of this study was to evaluate the effect of alder buckthorn (Frangula alnus) and European hazelnut (Coryllus avellana) admixtures in pine monocultures on the accumulation and vertical distribution of heavy metals in soil surface horizons. Ten sites each of pine stands admixed with alder buckthorn, pine stands admixed with European hazelnut, and pine monocultures in the Rybnik Forest District in southern Poland were selected for each variant. For the study, soil samples were taken from each site from the organic and mineral horizons and analysed for N and C contents, C/N ratio, pHH2O, base cation contents, and heavy metal contents. For all three stand compositions, the organic horizons showed a significant accumulation of heavy metals compared to the mineral horizons. Heavy metal content was correlated with pH, N and C contents, C/N ratio, and base cation contents. The admixture of shrubs, especially European hazelnut, positively affected soil organic matter quality and, consequently, the accumulation of heavy metals. European hazelnut had the most beneficial soil effects on the tested soil, which confirms that this species can be used in planning species composition, particularly in regions prone to heavy pollutant deposition.

Variability of Concentrations of Potentially Toxic Metals in the Topsoil of Urban Forest Parks (Southern Poland)

Forest ecosystems and urban parks are an integral part of the natural environment and the natural system of a city, where they form a mosaic of habitats resulting from the variation in soil cover due to human activities. The study was conducted in urban forests in five urban parks in Upper Silesia (southern Poland) and investigated the chemical properties and content of potentially toxic metals (PTMs) in the topsoil, which had an average thickness of 15 cm for all samples. The soil reaction ranged from acidic (pH = 4.7–5.1, in KCl) to slightly acidic (pH = 5.6, to neutral—6.6–7.2) at most sites. The organic carbon (OC) content was relatively high at all sites, ranging from 1.19 to 14.3%, with the highest total nitrogen (Nt) content being 0.481%. The average total phosphorus (Pt) content in the parks ranged from 310 mg kg−1 to 684 mg kg−1, while the highest values were 1840 mg kg−1. The total Cr, Cu, Co and Ni content was within acceptable limits, while the Zn, Pb, Cd, As, Sr and Ba exceeded acceptable standards. In terms of content, Zn dominated the PTMs at each site (Zn > Pb > Ba > Sr > Cu > Cr > As > Ni > Cd > Co), while Ba and Pb alternated in taking second place (Zn > Ba > Pb > Sr > Cu > Cr > Ni > As > Co > Cd). Environmental indicators, such as the geoaccumulation index (Igeo), enrichment factor (EF) and potential ecological risk index (RI), showed that the analyzed soils are highly contaminated with PTMs. Among the sources of pollution in the urban forest are low emissions from coal combustion, industrial activities, water runoff from streets and proximity to transportation routes. Identification of PTM levels in urban parks will provide valuable information on the behavior of these metals, which is important in sustainable development and can help evaluate the local spatial development plans of urbanized areas.

Evaluation of Water Quality from the Zimny Sztok Spring (Southern Poland)—Preliminary Results

Enabling citizens to have access to good-quality water is an important challenge in the 21st century. Water quality in springs located in urban areas may deteriorate as a result of natural and anthropogenic factors. The quality of water in springs is influenced not only by rock weathering, evapotranspiration and climate change but also by the lack of sustainable resource use. Anthropogenic activities, including industrial and domestic and agricultural activities, may not only lead to an increase in pollutants such as nitrates, chlorides and heavy metals but also to the development of dangerous bacteria. Research on water quality was conducted in two series of measurements at the Zimny Sztok spring (southern Poland), located in an urban space. The results of the analyses indicate that the waters of this spring, despite anthropogenic pressure, exceeded the permissible values for drinking water only in terms of the total number of microorganisms. Unfortunately, this was not confirmed by Water Quality Index (WQI) values. These were approximately equal to 99 in the first measurement series and about 41 in the second series. Maintaining appropriate water quality is one of the goals of rational water management, and the constant control of selected parameters in the water falls within the scope of the concept of smart city/smart water. The example of this spring shows that the water was used by residents without information about its current physicochemical parameters and bacteriological composition. This problem can be solved in the future by implementing a monitoring system for this point. The aim of this work was to examine the quality of water in the spring, use the WQI to determine the potential risks and present the general problem of people consuming water from urban sources without knowing about their parameters.

Groundwater Storage Variations across Climate Zones from Southern Poland to Arctic Sweden: Comparing GRACE-GLDAS Models with Well Data

The aim of this paper is to assess the correlation of groundwater level changes (or groundwater level anomalies (GWLA)) obtained from direct measurements in wells with groundwater storage anomalies (GWSA) calculated using Gravity Recovery and Climate Experiment (GRACE) products and Global Land Data Assimilation Systems (GLDAS) models across different climate zones, from temperate Poland to Arctic Sweden. We recognize that such validation studies are needed to increase the understanding of the spatio-temporal limits of remote sensing model applicability, not least in data-scarce sub-Arctic and Arctic environments where processes are complex due to the impacts of snow and (perma) frost. Results for temperate climates in Poland and southern Sweden show that, whereas one of the models (JPL_NOAH_GWSA) failed due to water balance term overestimation, the other model (CSR_CLM_GWSA) produced excellent results of monthly groundwater dynamics when compared with the observations in 387 groundwater wells in the region during 2003–2022 (cross-correlation coefficient of 0.8). However, for the sub-Arctic and Arctic northern Sweden, the model suitable for other regions failed to reproduce typical northern groundwater regimes (of the region’s 85 wells), where winter levels decrease due to the blocking effect of ground frost on groundwater recharge. This suggests, more generally, that conventional methods for deriving GWSA and its seasonality ceases to be reliable in the presence of considerably infiltration-blocking ground frost and permafrost (whereas snow storage modules perform well), which hence need further attention in future research. Regarding long-term groundwater level trends, remote sensing results for southern Sweden show increasing levels, in contrast with observed unchanged to decreasing (~10 mm/a) levels, which may not necessarily be due to errors in the remote sensing model but may rather emphasize impacts of anthropogenic pressures, which are higher near the observation wells that are often located in eskers used for water supply. For sub-Arctic and Arctic Sweden, the (relatively uncertain) trend of the remote sensing results nevertheless agrees reasonably well with the groundwater well observations that show increasing groundwater levels of up to ~14 mm/a, which, e.g., is consistent with reported trends of large Siberian river basins.

The Significance of the Financial Situation of Local Government Units for Their Energy Transition Activities: The Case of the Podkarpackie Region

This paper discusses the financial determinants of the efforts of local government units (LGUs) to invest in the area of energy transition (ET). The main objective is to verify the links between the scale, directions, and funding sources of LGUs’ investments in ET and their budgetary situation described by the level of income independence, budget result, and debt level. The general research hypothesis assumes that the ET investment activity of LGUs is associated with their financial situation. The analysis covers the period 2019–2022 and uses data from the questionnaire-based survey conducted in 2023 among 181 LGUs in the Podkarpackie region in southern Poland. Non-parametric tests were employed to verify the association between the LGUs’ ET investment activity and their financial situation: the Chi2 test, the Mann–Whitney U test, the Kruskal–Wallis test, and the Kendall’s Tau correlation. Financial aspects were shown to influence the scale of municipal ET investments, as well as the type of projects implemented. Among the analysed financial indicators, the debt level was the most important constraint for LGUs to make their own ET investments. The debt level not only determined the scale of municipal ET investment, but also influenced decisions on the type of investments.

Urban Flood Modeling for Sustainability Management: Role of Design Rainfall and Land Use

This study aimed to evaluate how different methods of determining design rainfall levels and land usage affect flood hydrographs in an urban catchment; specifically, the catchment in southern Poland. The data included daily precipitation records from 1981 to 2020 and land cover information from Corine Land Cover and Urban Atlas databases for 2006 and 2018. The analysis involved examining precipitation data, determining design rainfall levels, analyzing land usage databases, exploring the influence of design rainfall levels on hydrograph characteristics, and investigating the database’s impact on these characteristics. No discernible trend in precipitation was found. The highest design rainfall values followed the GEV distribution, while the lowest followed the Gumbel distribution. Both land usage databases indicated an increasing human influence from 2006 to 2018. This study conclusively showed that the method used for estimating design rainfall and the choice of the land usage database significantly affected hydrograph characteristics. Multivariate analyses are recommended for design rainfall assessments, while the Urban Atlas database is preferred for urban catchment land usage determinations due to its detailed information.

Comparison of nectar and pollen resources in various Tilia species. A case study from southern Poland

Management of food resources is considered fundamental for supporting insect pollinators, whose abundance shows a downward trend on the global scale. Here, the nectar and pollen production (per flower, per 1 m2 of tree crown), the composition of nectar carbohydrates and the levels of pollen proteins was evaluated in eight Tilia species (Malvaceae) growing in SE Poland. In the warm-summer continental climate, Tilia species can improve nectar and pollen resources mainly in June/July. Although the flowering period of each species is short (8.0–14.7 days), appropriate selection of species can extend the availability of food for more than a month. However, the considerable variations in the flowering onset (11.2–20.1 days) may cause significant inter-seasonal shifts in food accessibility.The nectar with its highly changeable sugar concentration (29.5–77.4%) was composed of sucrose, glucose, and fructose; protein content in pollen was 7.2–16.8%. The mean sugar yield was in the range of 0.95–19.1 g per 1 m2 (T. amuriensis – T. platyphyllos, respectively), whereas the mean pollen yield was 1.37–4.1 g per 1 m2 of tree crown (T. amuriensis – T. × euchlora, respectively). Significant year-to-year fluctuations of sugar and pollen yield in linden trees have to be taken into account in conservation schemes, and the introduction of other flowering plants with more stable sugar and pollen production should be considered in an area with a high density of linden trees. Linden flowers mainly support honey bees; nevertheless, wild pollinators (bumble bees, solitary bees, and dipterans) can also benefit from linden floral resources.

Assessment of the Effect of Meteorological Conditions on the Concentration of Suspended PM2.5 Particulate Matter in Central Europe

The purpose of this study was to use principal component analysis to determine the effect of meteorological elements on the concentration of PM2.5 particulate matter in Krakow, the capital of the Lesser Poland Voivodeship in southern Poland. Daily values for selected meteorological elements measured in spring, summer, autumn, and winter over a 10-year period, obtained from the Institute of Meteorology and Water Management—National Research Institute, were adopted as variables explaining PM2.5 concentrations. Data on particulate air pollution were obtained from the air monitoring station in Krakow. In spring, autumn, and winter, the first factor significantly influencing the PM2.5 concentration was the maximum, minimum, and average temperature. In summer, the average and maximum temperatures were significant. The second factor in spring was precipitation and wind speed, and the third was relative humidity. In summer, the second factor was atmospheric pressure, and the third was relative humidity. The second factor in autumn was atmospheric pressure and precipitation, and the third was relative humidity. In winter, the second factor was wind speed, and the third was precipitation and relative humidity. Throughout the study, the annual mean PM2.5 concentrations exceeded acceptable and target levels defined by the Regulation of the Minister of the Environment, and even further exceeded the level recommended by the WHO.

Cisplatin Monotherapy as a Treatment Option for Patients with HER-2 Negative Breast Cancer Experiencing Hepatic Visceral Crisis or Impending Visceral Crisis.

Hepatic visceral crisis (VC), characterized by a rapid total bilirubin increase with disease progression, poses a life-threatening risk in advanced breast cancer (ABC). International consensus guidelines define VC and touch on impending VC (IVC). Limited data exist on systemic treatments for hepatic VC/IVC. This study explores the safety and efficacy of cisplatin monotherapy in patients with Human Epidermal Growth Factor Receptor 2- negative breast cancer (BC) and hepatic IVC/VC. In this retrospective single-center cohort study data of patients treated with cisplatin monotherapy (60-80mg/m2, every 3-4weeks) between 2016 and 2023 at a reference Cancer Centre in Southern Poland were analyzed. 33 female patients (24/33 hormonal-positive) with the mean age 53.84years were included. Participants progressed on median 2 prior palliative systemic treatment lines. In 10/23 patients hepatic VC and in 23/33 IVC (rapid, symptomatic liver progression; extensive liver involvement; alanine or aspartate aminotransferase > 2 × normal limit; significant increases in lactate dehydrogenase, alkaline phosphatase, or gamma-glutamyl transferase) were identified. Median progression-free survival was 1.87months and median overall survival 2.67months. 33% of the patients presented stable disease or partial response. Eight patients experienced adverse events grade ≥ 3: in five the dose of cisplatin was reduced; two stopped the treatment. Due to the hepatotoxicity of BC-active drugs, specific recommendations for systemic treatment are scarce. Our study explored cisplatin's potential use, finding it to be a viable option in patients with performance status 0 or 1 experiencing hepatic IVC/VC, irrespective of liver function parameters and other factors.

ERT field survey supported with numerical and analogue modeling applied to study a fragment of the Pieniny Klippen Belt (Spisz Pieniny Mountains, southern Poland)

Electrical Resistivity Tomography (ERT) method was applied to study a fragment of the Pieniny Klippen Belt (PKB), which has been investigated for more than 100 years. The study area is located in the Spisz Pieniny Mountains of southern Poland. The PKB in this region includes a characteristic belt of limestone outcrops. ERT turned out to be an effective method to determine the structure of this part of the PKB, revealing its zonal nature and documenting the presence of limestone olistoliths and allowing estimates of their sizes. Moreover, we show that proper planning and conducting of ERT measurements in the field is critical to the effective use of resistivity data for geological inference and interpretation. This has been demonstrated by performing appropriate numerical and analogue ERT modeling that shows possible ambiguous results arising from the field ERT survey. Awareness of this issue can help researchers avoid and minimize false interpretation of ERT data.

Cisplatin monotherapy as a treatment option for patients with HER-2–negative breast cancer facing hepatic visceral crisis or impending visceral crisis: A single-center cohort study.

e13057 Background: The international consensus guidelines, ESO-ESMO's fifth edition, characterize visceral crisis (VC) in advanced breast cancer (ABC) as a swift rise in bilirubin during disease progression. The guidelines also briefly address the impending VC (IVC) definition. Systemic treatment data for hepatic VC/IVC are scarce and lacking in international guidelines regarding BC treatment. This study explores cisplatin monotherapy's safety and efficacy in HER2-negative BC patients with hepatic IVC/VC. Methods: In this retrospective cohort study conducted at a Reference Cancer Center in Southern Poland, data from HER2 negative ABC patients who underwent cisplatin monotherapy (60-80mg/m2, every 3-4 weeks) between 2016 and 2023 were examined. Hepatic VC followed ABC5 definition of rapid bilirubin elevation >1.5 times the upper limit of normal (ULN) due to diffuse liver metastases, in the absence of biliary tract obstruction or Gilbert's syndrome; IVC was characterized by rapid, symptomatic liver progression, a majority of liver involvement, with ALT or AST >2 x ULN, and significant increases in LDH, alkaline phosphatase, or GGTP. Results: The study included 33 female participants (24 hormonal positive) with a mean age of 53.8 years (range 34-77). Among them, 10 were identified with hepatic VC, and 23 with IVC. Median number of previous palliative systemic treatment lines was 2 (range: 0-5). The median progression-free survival was 1.87 months and the median overall survival was 2.67 months. Patients received median 1 cycle of cisplatin (1-6): 17 patients received only one cycle, 6 patients two cycles and 10 patients ≥three cycles. Using the Conditional Inference Trees algorithm allowed to assess that patients with performance status (PS) 0 or 1 (15 patients) had a 66.67% chance of achieving stable disease (SD) or partial regression (PR) as the best response, while patients with PS ≥2 (18 patients) had a 5.6% chance of achieving SD or PR as per Response Evaluation in Solid Tumors 1.1 Criteria (median time from cisplatin initiation to imaging studies 2.23 months). Eight patients experienced adverse events of grade ≥3, such as fatigue or nephrotoxicity leading to dose reduction in 5 cases and treatment discontinuation in 2. Conclusions: The differentiation between IVC and VC lacks precision. Due to the hepatotoxicity of BC-active drugs, specific recommendations for systemic treatment are lacking. Our study explored cisplatin's potential use, finding it to be a feasible choice in patients with PS ≤1 experiencing hepatic IVC/VC, regardless of liver function parameters. Almost one-third of our patients were administered treatment in the last 30 days of life, potentially adversely affecting their quality of life and causing unwarranted suffering and costs. Academic clinical trials should be organized to address this pressing medical gap.

Limited evidence for genetic differentiation or adaptation in two amphibian species across replicated rural-urban gradients.

Urbanization leads to complex environmental changes and poses multiple challenges to organisms. Amphibians are highly susceptible to the effects of urbanization, with land use conversion, habitat destruction, and degradation ranked as the most significant threats. Consequently, amphibians are declining in urban areas, in both population numbers and abundance, however, the effect of urbanization on population genetic parameters remains unclear. Here, we studied the genomic response to urbanization in two widespread European species, the common toad Bufo bufo (26 localities, 480 individuals), and the smooth newt Lissotriton vulgaris (30 localities, 516 individuals) in three geographic regions: southern and northern Poland and southern Norway. We assessed genome-wide SNP variation using RADseq (ca. 42 and 552 thousand SNPs in toads and newts, respectively) and adaptively relevant major histocompatibility complex (MHC) class I and II genes. The results linked most of the genetic differentiation in both marker types to regional (latitudinal) effects, which also correspond to historical biogeography. Further, we did not find any association between genetic differentiation and level of urbanization at local scales for either species. However, urban smooth newts, but not toads, have lower levels of within-population genome-wide diversity, suggesting higher susceptibility to the negative effects of urbanization. A decreasing level of genetic diversity linked to increasing urbanization was also found for MHC II in smooth newts, while the relationship between MHC class I diversity and urbanization differed between geographic regions. We did not find any effects of urbanization on MHC diversity in the toad populations. Although two genetic environment association analyses of genome-wide data, LFMM and BayPass, revealed numerous (219 in B. bufo and 7040 in L. vulgaris) SNPs statistically associated with urbanization, we found a marked lack of repeatability between geographic regions, suggesting a complex and multifaceted response to natural selection elicited by life in the city.

Crustal deformation from GNSS measurement and earthquake mechanism along Pieniny Klippen Belt, Southern Poland

The dynamic geological features of the Pieniny Klippen Belt (PKB) in southern Poland are nowadays a focal point of researchers as it is recognised as an active zone of crustal discontinuity. In the present study, we employed long-term analysis of ground-based global navigation satellite system (GNSS) measurements (from 2004 to 2020) to probe the crustal deformation, strain rates, and rotational rates in the PKB unit and the surrounding region, i.e., Magura Nappe (MN) and Podhale Flysch (PF) units. Measured velocities from GNSS observables are modelled by the auto-regressive integrated moving average (ARIMA) method to comprehend the long-term tectonic deformation. Our results showed that the ARIMA-modelled velocity varied from ~ 0.15 to ~ 8.86 mm/yr, indicating about 8.71 mm/yr difference along all units. Such differences suggest that crustal slip along the active thrusts and folds is the major factor causing regional deformation. The strain rates in PKB are also varying from the western to the eastern part. The rotational rates in PKB show a counterclockwise (CCW) pattern similar to the strain rates. These patterns suggesting that the PKB was rotated in the CCW direction with a large angle during the Miocene period. Finally, we analysed the seismicity for a period from 2004 to 2020 by using Bayesian moment tensor inversion and multivariate Bayesian inversion. The Bayesian inversion was applied based on bootstrapping chain analysis to figure out the earthquake mechanism using moment tensor inversion for the mainshock that occurred in Poland on 20 July 2018. The inversion results for the 2018 earthquake resolved a thrusting mechanism with nodal plane-1 having a strike of 346°, dip of 32°, and rake of 92° and a nodal plane-2 with a strike of 163°, dip of 58°, and rake of 89°. Since the seismicity in the Poland region has experienced less significant earthquakes in the last century, it is reasonable to attribute this lower seismic activity to the correspondingly low slip rates discerned through geodetic monitoring efforts.

Where have Ukrainian refugees gone? Identifying potential settlement areas across European regions integrating digital and traditional geographic data

AbstractThe escalation of conflict in Ukraine has triggered the largest refugee crisis in Europe since WWII. As of early April 2024, over 5.9 million people have fled Ukraine. Large‐scale efforts have been made to identify the major receiving countries. However, less is known about the subnational areas within host countries where refugees have migrated. Identifying these areas is key for the appropriate allocation of humanitarian aid. By combining digital Facebook API data and traditional data from Eurostat, this paper aims to identify and characterise potential settlement areas of Ukrainians across the main destination countries in Europe. We identify high concentrations of Ukrainians in urban areas with a preexisting diaspora and tight labour market conditions across southern, northern‐west and central Poland and the city of Prague in the Czech Republic. We also find potential settlements in key urban agglomerations with a moderate diaspora and high levels of unemployment in Spain. Only in Romania, refugees seem to have settled in rural areas which show a moderate diaspora but low levels of unemployment. Potential settlement areas in Germany, Italy and the United Kingdom are spread across the country. Surprisingly, we do not identify potential settlement areas in bordering regions with Ukraine within neighbouring countries, suggesting that refugees may have used them as transit points. Our findings point out that different packages of humanitarian assistance may be needed according to the number of refugees and the characteristics of settlement areas.

Bacterial communities inhabiting the ascomata of the ectomycorrhizal summer truffle (Tuber aestivum)

This paper continues the studies on the role of bacteria in the environment of summer truffle (Tuber aestivum), focusing on ascomata at three sites in the Nida Basin (southern Poland). Bacterial taxa and the number of OTU sequences identified were determined based on analysis of peridium and gleba collected in 2017 and 2018. The aim of the presented work is to learn the structure of bacterial communities associated with the ascomata of the summer truffle T. aestivum forming in specific stands in south Poland and fine roots of surrounding trees. Sanger DNA sequencing, and next-generation sequencing (NGS) were used. NGS sequencing platforms short-read (300 bases) was selected, using a MiSeq Illumina. The Kruskall-Wallis test was used for alpha diversity analysis and the PERMANOVA test for beta diversity. Alpha and beta biodiversity analyzes were performed using various indices (Chao1, Simpson, Shannon, Faith's phylogenetic, observed OTUs, Heip's evenness measure - alpha diversity; UniFrac, Jaccard, Bray–Curtis - beta diversity).The Sanger method identified six bacterial taxa that were dominant in the community: Bacillus (86.7 %), Pseudomonas (51.5 %), Stenotrophomonas (15.5 %), Paenibacillus (7.9 %), Rhizobium (7.3 %), and Chryseobacterium (8.5 %). In contrast, NGS results showed the dominance (at OTUs >10,000) of taxa: Bradyrhizobiaceae, Sphingobacteriaceae, Micrococcaceae, Yersiniaceae, Sphingomonadaceae, Rhizobiaceae, Flavobacteriaceae, and Comamonadaceae. The PERMANOVA test for the Bray-Curtis and Jaccard indices showed dissimilarities between samples from two of the sites with respect to beta diversity. Significant differences were found in the bacterial communities colonizing the ascomata of T. aestivum and in the root communities with T. aestivum ectomycorrhizae the purpose of this study. The results obtained may be helpful in selecting and monitoring sites suitable for the formation of ascomata of T. aestivum.

Mine Wastewater Effect on the Aquatic Diversity and the Ecological Status of the Watercourses in Southern Poland

Coal mining activity contributes to energy security and employment occupation, but is associated with environmental deterioration. Coal combustion leads to GHG emissions, while coal mining results in the generation of saline effluents. These effluents are discharged in inland surface waters, applying significant pressure on their quality, with a negative impact on aquatic life and the economy of a region. This study includes water samples that were analyzed in order to investigate the organic compounds, heavy metals, and other physicochemical parameters. Biological monitoring was done according to the Water Framework Directive methodology. The results from an aquatic area in Southern Poland, which indirectly receives coal mine effluents, indicate elevated salinity with excessive chlorides, sulfates, and sodium ions. The water quality of another non-polluted aquatic area was also assessed to examine the impact of indirect coal mine wastewater discharge on this area. The high salinity levels hinder the use of river water for drinking, agricultural, or industrial purposes. The results obtained show high pressure on the ecological status of streams and rivers that receive mine effluents, and on the density and diversity of aquatic invertebrates. This pressure is clearly visible in the structure of benthic communities and in invertebrate diversity. It also contributes to the appearance of invasive species and increasing water salinity. Limiting discharges of mine water transporting large loads of saline substances would reduce the negative impact on the quality of river waters and biological life.

Black Crust from Historic Buildings as a Natural Indicator of Air Pollution: A Case Study of the Lipowiec Castle, Babice, Southern Poland

The study is focused on the analysis of black crust and soiling on the building materials of the medieval Lipowiec Castle in southern Poland. The castle was constructed using local, partly dolomitic limestones and dolomites, supplemented with other limestones and bricks, during 20th-century renovations of the castle ruins. The crust and soiling components, secondary mineral phases, and particulate matter of anthropogenic origin were analysed using Raman micro-spectroscopy (RS) and scanning electron microscopy coupled with energy-dispersive spectrometry (SEM-EDS). The crust, mostly composed of gypsum and other sulphate phases, was found to contain carbonaceous matter, spherical Si-Al glass particles, and iron oxides, with admixtures of other elements, including heavy metals, as well as irregularly shaped particles containing various metals. These components reflect the air pollution in the region, related to the combustion of solid fuels in both industrial power plants and local domestic furnaces, Zn-Pb ore mining (operational until 2021), and smelting in the neighbouring industrial centre. Despite its location in a rural area, the castle has been exposed to pollution for an extended period due to its proximity to large industrial centres. Therefore, the crust analysed may serve as an environmental indicator of the nature of the air pollution in the region.