Southeastern Coast Research Articles

A comprehensive genome survey study unveils the genomic characteristics and phylogenetic evolution of fishes in the Uranoscopidae family

The construction of high-quality genomes is fundamental for molecular studies of species. Currently, there are no published genome sequences within the Uranoscopidae family, and research on the genomic characteristics of this family is lacking. In this study, genomic analyses of seven species from the Uranoscopidae family which captured from the southeastern coast of China were conducted using next-generation sequencing technology. The results revealed that the genomic characteristics of the seven species are relatively similar. The genome sizes of the seven Uranoscopidae species ranged from 536.00 Mb to 652.49 Mb, with repeat sequence proportions between 20.09% and 36.64%, and heterozygosity levels ranging from 0.41% to 0.88%. The assembled draft genomes exhibited GC contents of these species ranging from approximately 42.14% to 43.53%. Furthermore, the mitochondrial sequences for the seven species were assembled, with sizes ranging from 14,966 to 18,446 bp. The evolutionary relationships between different species were depicted through the mapping of conserved genes, whole-genome variances and mitochondrial genome sequences. Besides, the historical effective population sizes of all species experienced rapid expansion after the Last Interglacial Period, with three species undergoing bottleneck effects. In conclusion, this study provides a reference for the subsequent construction of high-quality genomes for species within the Uranoscopidae family and offers a rich data resource for further evolutionary research.

Regional Intraspecific Differences of Thermal Biology in a Marsupial Hibernator.

AbstractDuring periods of torpor, hibernators can reduce metabolic rate (MR) and body temperature (Tb) substantially. However, to avoid physiological dysfunction at low temperatures, they defend Tb at a critical minimum, often between ~0°C and 10°C via an increase in MR. Because thermoregulation during torpor requires extra energy, individuals with lower Tb's and thus minimal MR during torpor should be selected in colder climates. Such inter- and intraspecific variations occur in some placental mammals, but for the evolutionary separate marsupials, available information is scarce. Marsupial eastern pygmy possums (Cercartetus nanus; ~22 g body mass), widely distributed along the Australian southeastern coast including subtropical to alpine areas, were used to test the hypothesis that the defended Tb of torpid individuals is related to the climate of their habitat. Possums were captured from five regions, 1,515 km apart, with midwinter (July) minimum environmental temperatures (min Tenv's) ranging from -3.9°C to 6.6°C. Captive possums in deep torpor were slowly cooled with ambient temperature (Ta), while their MR was measured to determine the minimum torpor metabolic rate (TMR), the Ta at which their MR increased for thermoregulation (min Ta), and the corresponding minimum Tb (min Tb). Partial least squares regression analysis revealed that Ta and Tenv were the strongest explanatory variables for the min Tb. The min Tb and Ta were also correlated with latitude but not elevation of the capture sites. However, the best correlations were observed between the min Tenv and the min Tb and Ta for individuals experiencing min ; these individuals thermoconformed to min Ta's between -0.8°C and 3.7°C, and their min Tb ranged from 0.5°C to 6.0°C and was 0.5°C-2.6°C below the min Tenv at the capture site. In contrast, individuals experiencing a min Tenv of -3.9°C regulated Tb at or 4.5°C above the Tenv. The minimum TMR of all possums did not differ with Ta and thus did not differ among populations and was 2.6% of the basal MR. These data provide new evidence that thermal variables of marsupials are subject to regional intraspecific variation. It suggests that min Tb is a function of the min Tenv but only above 0°C, perhaps because the differential for torpid possums in the wild, at a min Tenv of -3.9°C, remains small enough to be compensated by a small increase in MR and does not require the physiological capability for a reduction of Tb below 0°C.

An analysis of the aesthetic characteristics of the Guangxi folk tale “The Legend of Mazu”

The Mazu belief is prevalent along the southeastern coast of China and has significant influence both domestically and internationally. In Guangxi, this tradition dates back to the Ming and Qing dynasties and has integrated with local cultural elements to form a unique belief system. The Mazu Festival, held once every three years, showcases distinct characteristics in the portrayal of Mazu and related objects. These distinctions are evident in clothing, body shaping, and line expression, all of which reflect the local aesthetics of Guangxi. Summarizing the aesthetic features of this tradition can be effectively applied to the expression of regional artworks.

Nonstationarity in Extreme Precipitation Return Values along the U.S. Gulf and Southeastern Coasts

Abstract This study estimates extreme rainfall trends across the Gulf Coast and southeastern coast of the United States while applying methods for extending the temporal record and aggregating across spatial trend variations. Nonstationary generalized extreme value (GEV) models are applied to historical annual daily maximum precipitation data (1890–2019) while using CMIP5 global mean surface temperature (GMST) as the covariate. County composites and multicounty regions are used for local data record extension and pooling. Unlike most previous studies, return periods as long as 100 years are analyzed. The local trend estimates themselves are found to be too noisy to be reliable as estimates of climate-driven trends. However, application of a Gaussian process model to the spatial distribution of observed trends yields overall trend detection at the 95% significance level. The overall historical increase due to nonstationarity across the study region, with associated 95% confidence intervals, is 9% (3%, 15%) for the 2-yr return period and 16% (4%, 26%) for the 100-yr return period. A trend is also detectable in the Gulf Coast subregion, but not in the smaller southeast subregion. Recent weather events and nonstationarity have caused the official return value estimates for parts of North and South Carolina to be much lower than the return values estimated here. Significance Statement Protection of people and infrastructure from flooding relies on accurate estimates of potential extreme rainfall intensity. Some official estimates of extreme rainfall near the Gulf Coast and southeastern coast of the United States are over 20 years old. We show that, across this region, there is a clear trend in daily rainfall so extreme that it only has a 1% chance of happening in any given year (the so-called 100-yr rainfall). This trend means that many existing estimates of extreme rainfall are too low, both now and in the future, so flooding risks based on those estimates would be underestimated as well.

Performance study of a photovoltaic system operating on the southeastern coast of Brazil

Performance study of a photovoltaic system operating on the southeastern coast of Brazil

Incredible Host Diversity and Regional Potential Distribution of an Oriental Parasitic Plant (Taxillus yadoriki)

This study investigates the host diversity and characteristics of the rare parasitic plant Silverberry-like taxillus (Taxillus yadoriki). Seogwipo, Jeju Island, where T. yadoriki grows naturally, was examined to clarify the diversity of host species, their location, and the part of the tree where T. yadoriki is attached. A total of 687 host trees were found. Taxillus yadoriki was intensively distributed in the lowlands below 200 m a.s.l. The host trees belonged to 40 species, four varieties, three cultivars, and two hybrids from 32 genera and 21 families, of which 23 taxa were identified as host plants for the first time in this study. Incredibly, this plant was found to be able to parasitize a variety of plants from 12 orders. Taxillus yadoriki was found to be parasitic in three orders of Gymnosperms, Ginkgoales, Cupressales, and Pinales, as well as nine orders of Angiosperms, including Magnoliales, Rosales, and Ericales. Rosaceae was the most diverse at 18.0%, and most species were broad-leaved and arboreal. Among them, Cryptomeria japonica and Litsea japonica had the highest frequency of parasitization and T. yadoriki was mainly attached to the upper part of the main tree and the main branch; as a result, T. yadoriki was distributed and showed specificity in the position of disturbance on the host. T. yadoriki’s specific distribution model was built as a Maxent program. The area under the curve of the receiver operation characteristic was 0.948 ± 0.026 (mean ± standard deviation), and the performance of the constructed model was excellent. Of the total eight environmental factors, density of artistic forest and meant temperature of coldest quarter accounted for 75.3% of the total importance, which was the main distribution determinant of species. In the average distribution probability map of T. yadoriki, the critical probability for determining distribution was calculated to be 0.2898 ± 0.1018. Accordingly, the distribution of species is predicted to expand from Jeju Island to the entire southern and southeastern coasts.

Characteristics of surface urban heat islands in global cities of different scales: Trends and drivers

Given the increasing severity of the surface urban heat island (SUHI) phenomenon, urban residents face heightened heat stress. Consequently, mitigating the effects of SUHI becomes critically important to improve urban livability. Previous studies often overlooked the influence of city size on SUHI, focusing on the correlational between SUHI and drivers and sidelining the synergistic effects of multiple factors on SUHI. To address this issue, we introduced a novel method, “geographical convergent cross-mapping”, coupled with the random forest and Shapley value theory. Our results indicated that: Globally, SUHI exhibited a decreasing trend during day and night, with significant spatiotemporal disparities, and in cities of varying sizes, the cooling capacity of vegetation for SUHI showed significant variation. Locally, in arid cities spanning 150–200 km², vegetation contributed to cooling SUHI by up to 61 %, but this effect lessened in larger cities. Notably, in equatorial cities larger than 200 km², this capability dropped drastically during summer, from 49 % to 7 %. Long-term trends showed an increase in daytime SUHI in equatorial regions and large cities along the southeastern coasts of China and the United States. This study provides key insights for precise urban climate adaptation and sustainable planning in cities of different sizes.

On the occurrence of three blennid species in the South-eastern Mediterranean coast of Turkey

In the present study, three blennid species of Aidablennius sphinx (Valenciennes, 1836) Parablennius tentacularis (Brünnich, 1768) and Scartella cristata (Linnaeus, 1758) were reported from the southeastern Mediterranean waters with visual records during an underwater survey conducted on October 19, 2015 in the Konacik and, on July 30, 2016 in the Çevlik coast at a depth ranges of 2-10 m. The present report is the occurrence and is the first confirmation of three blennid species from Iskenderun Bay, Turkey (South-eastern Mediterranean Sea). Besides, this study will be useful fisheries biology and ecology and also contribute to fisheries scientists.Keywords: Blenniidae, Combtooth blennies; Iskenderun Bay; Mediterranean Sea; observation

Assessment of the combined vulnerability to droughts and heatwaves in Shandong Province in summer from 2000 to 2018.

Droughts and heat waves exhibit synergistic effects and are among the world's most costly disasters. To explore the spatiotemporal differences and formation mechanisms of the combined vulnerability to droughts and heat waves in Shandong Province over the past 20years, a vulnerability scoping diagram (VSD) model with three dimensions-exposure, sensitivity, and adaptability-was constructed to assess and compare the combined vulnerability to high-temperature and drought events, considering economic and social conditions. The results showed that (1) over the past 20years, heat waves and droughts have increased in Shandong Province. The number of high-temperature events significantly increased in the west and decreased along the eastern coast, and drought change was characterized by an increase in the south and a decrease in the north. (2) The combined exposure to summer droughts and heat waves in Shandong Province showed a significant increasing trend (P < 0.05) at a rate of approximately 0.072/10a; the combined sensitivity significantly decreased (P < 0.05) at a rate of approximately 0.137/10a, and the combined adaptability continued to increase at a rate of approximately 0.481/10a. (3) The combined vulnerability to summer droughts and heat waves in the western inland area of Shandong Province was high and gradually decreased toward the southeastern coast. The overall decrease trend was nonsignificant with a decrease of approximately 0.126/10a, and the decline rate decreased from northwest to southeast, in which Laiwu, Yantai, Jinan, and Zibo cities exhibited a significant decreasing trend (P < 0.05). Although the compound vulnerability of Shandong Province has decreased insignificantly, the frequency of combined drought and heat wave events has increased, and the combined vulnerability will increase in the future.

Morphology and morphometry of morphotypes in the population of Artemia franciscana (Kellogg, 1906) from salterns of the southeastern coast of India

We document the morphology, morphometric variations among the morphotypes of the brine shrimp, Artemia franciscana. From the samples collected at four different locations in South India, Tamil Nadu viz. Kelambakkam, Vedaranyam, Tuticorin and Nagarcoil we identified six morphotypes: M1, M3, M4 in males and F1, F2, F3 in females. The Scanning electron micrographs of male morphotypes show distinct variation in the basal width, shape and number of cuticular cones on the second antennae. Similarly, the female morphotypes show various shape and sizes of the ovisac with or without spines. However, the cyst surface topography is smooth without any specific variation/ornamentation in all three female morphotypes. Multivariate analysis of eighteen morphological traits measured in males and fifteen in females to elucidate the intraspecific variations among morphotypes indicate significant dissimilarity between males and females. Furthermore, relative length measurements showed distinct morphometric variation of traits between the morphotypes encountered at different sampling sites.

Evaluating Sea Level Rise Impacts on the Southeastern Türkiye Coastline: a Coastal Vulnerability Perspective

Coastal areas are inherently sensitive and dynamic, susceptible to natural forces like waves, winds, currents, and tides. Human activities further accelerate coastal changes, while climate change and global sea level rise add to the challenges. Recognizing and safeguarding these coasts, vital for both socioeconomic and environmental reasons, becomes imperative. The objective of this study is to categorize the coasts of the Mersin and İskenderun bays along the southeastern coast of Türkiye based on their vulnerability to natural forces and human-induced factors using the coastal vulnerability index (CVI) method. The study area encompasses approximately 520 km of coastline. The coastal vulnerability analysis reveals that the coastal zone comprises various levels of vulnerability along the total coastline: 24.7% (128 km) is categorized as very high vulnerability, 27.4% (142 km) as high vulnerability, 23.7% (123 km) as moderate vulnerability, and 24.3% (126 km) as low vulnerability. Key parameters influencing vulnerability include coastal slope, land use, and population density. High and very high vulnerability are particularly prominent in coastal plains characterized by gentle slopes, weak geological and geomorphological features, and significant socioeconomic value.

Assessing metal(loid)s-Induced long-term spatiotemporal health risks in Coastal Regions, Bay of Bengal: A chemometric study.

Despite sporadic and irregular studies on heavy metal(loid)s health risks in water, fish, and soil in the coastal areas of the Bay of Bengal, no chemometric approaches have been applied to assess the human health risks comprehensively. This review aims to employ chemometric analysis to evaluate the long-term spatiotemporal health risks of metal(loid)s e.g., Fe, Mn, Zn, Cd, As, Cr, Pb, Cu, and Ni in coastal water, fish, and soils from 2003 to 2023. Across coastal parts, studies on metal(loid)s were distributed with 40% in the southeast, 28% in the south-central, and 32% in the southwest regions. The southeastern area exhibited the highest contamination levels, primarily due to elevated Zn content (156.8 to 147.2 mg/L for Mn in water, 15.3 to 13.2 mg/kg for Cu in fish, and 50.6 to 46.4 mg/kg for Ni in soil), except for a few sites in the south-central region. Health risks associated with the ingestion of Fe, As, and Cd (water), Ni, Cr, and Pb (fish), and Cd, Cr, and Pb (soil) were identified, with non-carcinogenic risks existing exclusively through this route. Moreover, As, Cr, and Ni pose cancer risks for adults and children via ingestion in the southeastern region. Overall non-carcinogenic risks emphasized a significantly higher risk for children compared to adults, with six, two-, and six-times higher health risks through ingestion of water, fish, and soils along the southeastern coast. The study offers innovative sustainable management strategies and remediation policies aimed at reducing metal(loid)s contamination in various environmental media along coastal Bangladesh.

Hematology and Serum Biochemistry of Coastal Seabirds Rehabilitated on the Southeastern and Southern Coast of Brazil.

Complete blood counts (n=566) and serum biochemistry (n=426) were assessed in seven coastal seabirds species that underwent rehabilitation along the southeastern and southern coast of Brazil from Saquarema, Rio de Janeiro State (22°56'16.44″S, 42°18'24.16″W) to Laguna, Santa Catarina State (28°29'43″S, 48°45'39.2″W), from August 2016 to August 2020. Blood samples were collected from four species of Charadriiformes, including Kelp Gull (Larus dominicanus, n=136), South American Tern (Sterna hirundinacea, n=25), Cabot's Tern (Thalasseus acuflavidus, n=17), and Common Tern (Sterna hirundo, n=14) as well as three species of Suliformes, the Brown Booby (Sula leucogaster, n=212), Magnificent Frigatebird (Fregata magnificens, n=104), and Neotropic Cormorant (Nannopterum brasilianum, n=58). The individuals were sampled as part of the protocol required before their release into the wild when considered healthy. This work aimed to establish the normal hematologic and biochemical reference values of those seabird species and, when possible, to analyze variations among age class and sex and to compare those with the available data in the literature. In addition, we provide the first baseline data for the South American Tern, Cabot's Tern, and Neotropic Cormorant. Baseline hematologic data are crucial for assessing health status of individuals and to support management and conservation actions, including release of seabirds into the wild.

Purification, structural characterization, and neuroprotective effect of 3,6-diisobutyl-2,5-piperazinedione from Halomonas pacifica CARE-V15 against okadaic acid-inducedneurotoxicity in zebrafish model.

Halomonas pacifica CARE-V15 was isolated from the southeastern coast of India to determine its genome sequence. Secondary metabolite gene clusters were identified using an anti-SMASH server. The concentrated crude ethyl acetate extract was evaluated by GC-MS. The bioactive compound from the crude ethyl acetate extract was fractionated by gel column chromatography. HPLC was used to purify the 3,6-diisobutyl-2,5-piperazinedione (DIP),and the structure was determined using FTIR and NMR spectroscopy. Purified DIP was used in an in silico molecular docking analysis. Purified DIP exhibits a stronger affinity for antioxidant genes like glutathione peroxidase(GPx), glutathione-S-transferase(GST), and glutathione reductase(GSR). Using in silco molecular docking analysis, the protein-ligand binding affinities of GSR (-4.70 kcal/mol), GST (-5.27 kcal/mol), and GPx (-5.37 kcal/mol) were measured. The expression of antioxidant genes were investigated by qRT-PCR. The in vivo reactive oxygen species production, lipid peroxidation, and cell death levels were significantly (p ≤ 0.05) increased in OA-induced group, but all these levels were significantly (p ≤ 0.05) decreased in the purified DIP pretreated group. Purified DIP from halophilic bacteria could thus be a useful treatment for neurological disorders associated with oxidative stress.

Assessing the effect of coastal upwelling on the air temperature at the south-eastern coast of the Baltic Sea

Coastal upwelling along the SE Baltic Sea coast is a common feature, especially during the warm season. It significantly lowers sea surface temperature (SST) in the coastal areas, and, therefore, may be responsible for modifying meteorological conditions in those coastal areas, where upwelling is most frequently observed. This study aims to assess the effect of coastal upwelling on the air temperature at the south-eastern coast of the Baltic Sea based on long-term period observations (2002–2021) from coastal hydrometeorological stations and satellite data. Overall, our study revealed that due to its high frequency and spatial extent, upwelling is responsible for lowering the mean summer season SST of the SE Baltic Sea coast by about 1°C. And even though upwelling is a short-term event, upwelling-induced SST drop results in cooling air temperatures in the coastal areas, i.e., the mean air temperatures during upwelling are typically 2−4°C lower than before. It was also observed that upwelling is favouring the development of advective fog. Thus, sudden changes in meteorological parameters during upwelling can have versatile effects on various socio-economic activities. The results of this study contribute to the understanding of upwelling feedback onto the lower atmosphere and, therefore, are important for advancing the accuracy of weather forecasts that are needed for coastal communities, including marine and coastal industries.

Habitat characterization, anthropogenic impacts and conservation of rhodolith beds off southeastern Malta

Abstract Rhodolith beds, including maerl, are structurally complex perennial habitats that support a high species diversity but are threatened by numerous human activities, particularly in the Mediterranean Sea. Despite their global ecological importance, increased research efforts are needed to facilitate development of effective measures to conserve these habitats. Two areas hosting rhodolith beds are known to occur off the coast of Malta (central Mediterranean), but only one has been previously studied. Through analysis of video footage collected from 56 different stations coupled with a desk study on human activities, the present study characterized the rhodolith bed located off the southeastern coast of Malta, including its extent, rhodolith morphotype composition, associated megabiota and impacts of anthropogenic activities thereon. The bed occurred at depths of 60–95 m and covered an area of approximately 200 km2, making it the second most extensive rhodolith area reported for the Mediterranean to date. It was dominated by spherical and branched rhodoliths whose cover reached a maximum of 74% but was more often &lt;50%, similar to other Mediterranean rhodolith beds. A total of 84 different megafaunal species were recorded, suggesting that the diversity of megafauna associated with rhodolith bed habitats has been previously underestimated. A number of human activities, including extensive vessel anchoring and officially designated areas for aquaculture, bunkering and trawling, overlap with the mapped rhodolith distribution, highlighting the risk of habitat degradation. A holistic approach to the management of all the competing activities and interests relative to the southeastern coast of Malta that gives due consideration to this newly characterized rhodolith bed and the threats it faces is therefore recommended. The legal framework and policy recommendations for better conservation of rhodolith bed habitats in the Mediterranean and European Seas are also discussed.

Fishery, population dynamics and stock assessment of flat needlefish Ablennes hians (Valenciennes, 1846) from the south-eastern Arabian Sea off Kerala coast, India

The age, growth, mortality parameters and stock status of Ablennes hians(Valenciennes, 1846) were assessed by using length frequency, catch, and effort datacollected from the commercial catches along the coast of Kerala (south-eastern ArabianSea) during 2015-2017. The growth parameters, L∞, K and t0 were estimated at 133.9 cm TL,0.72 yr-1 and -0.0026 yr, respectively. The instantaneous natural mortality (M), total mortality(Z), fishing mortality (F) and current exploitation rate (Ecur) were estimated as 0.95 yr-1,2.78 yr-1, 1.83 yr-1 and 0.66, respectively. Length at capture (Lc50) was estimated at 77.58cm TL, which is greater than Lm50. Beverton and Holt yield per recruit analysis, indicated thatmaximum Y/R could be obtained at an exploitation rate (Emax) of 0.75, suggesting scope foran increase in exploitation. In this analysis, spawning stock biomass (SSB) was not takeninto consideration, which is essential for maintaining recruitment in the future. In the presentstudy, E0.1 (0.61) and Ecur (0.66) were nearly equal, indicating that the fishery is running atan economic reference point i.e., maximum economic yield (MEY). Thompson and Bellprediction model showed that at the current level of exploitation, the SSB was reduced by20.49% and hence, there is no scope for further increase in fishing efforts to maintain the SSBat a relatively safe and sustainable level. The analysis revealed the maximum sustainableyield (MSY) of this species as 90.26 t, which can be obtained by increasing the present levelof fishing effort by 1.3 times but would result in the depletion of the SSB to a critically lowlevel of 15.65%. Therefore, a precautionary safe level of exploitation (E0.1), estimated at 0.61for the species, is recommended. Keywords:Belonidae, Fishery management, Population dynamics,Spawning stock biomass, Stock status

Monitoring dynamic mangrove landscape patterns in China: Effects of natural and anthropogenic forcings during 1985–2020

Many mangrove forests located along estuaries and deltas worldwide are currently facing irreparable losses caused by natural factors and intensive anthropogenic disturbances. The limited capabilities of traditional remote sensing processing platforms tend to lead to poor image processing consistency in medium- and large-scale areas, resulting in lower ground object classification accuracy. Consequently, little information is available regarding the extent and causes of variation in large-scale mangrove forests. In this study, we used Google Earth Engine mangrove forest data from eight periods between 1985 and 2020, with total accuracy rates of 82.95–90.15%, and combined landscape metrics with a centroid transfer model to quantitatively analyze the landscape evolution of mangrove forests in China. Finally, we employed a geodetector based on optimized parameters to quantitatively determine the potential mechanisms driving mangrove landscape evolution. Overall, a slight increase followed by a sharp decrease in mangrove forest area in China during the past 40 years, with a turning point around 2000 and substantial shifts in mangrove spatial patterns in Guangdong and Guangxi Provinces, particularly in the Pearl River Estuary and Beibu Gulf. The landscape pattern of Chinese mangrove forests has become fragmented and isolated through rapid urbanization in recent decades. Mangrove forests shifted significantly (> 31 km) early in the study period, followed by smaller shifts later in the study period. The optimal spatial scale of mangrove landscape driver detection was 270 m × 270 m. The variation in mangrove forest area was caused by the interactions of multiple factors, particularly between mean annual precipitation and other potential influencing factors. Government policies guided the conservation and management of mangrove forests in China to varying degrees throughout the study period. Our findings on the dynamics of mangrove forests provide a reference for integrated coastal management, which will contribute to the ecological utilization and high-quality development of coasts during extensive urbanization along the southeastern coast of China and similar large estuary deltas worldwide.

Expansion and transmission dynamics of high risk carbapenem-resistant Klebsiella pneumoniae subclones in China: An epidemiological, spatial, genomic analysis

AimsCarbapenem-resistant Klebsiella pneumonia (CRKP) is a global threat that varies by region. The global distribution, evolution, and clinical implications of the ST11 CRKP clone remain obscure. MethodsWe conducted a multicenter molecular epidemiological survey using isolates obtained from 28 provinces and municipalities across China between 2011 and 2021. We integrated sequences from public databases and performed genetic epidemiology analysis of ST11 CRKP. ResultsAmong ST11 CRKP, KL64 serotypes exhibited considerable expansion, increasing from 1.54% to 46.08% between 2011 and 2021. Combining our data with public databases, the phylogenetic and phylogeography analyses indicated that ST11 CRKP appeared in the Americas in 1996 and spread worldwide, with key clones progressing from China’s southeastern coast to the inland by 2010. Global phylogenetic analysis showed that ST11 KL64 CRKP has evolved to a virulent, resistant clade with notable regional spread. Single-nucleotide polymorphism (SNP) analysis identified BMPPS (bmr3, mltC, pyrB, ppsC, and sdaC) as a key marker for this clade. The BMPPS SNP clade is associated with high mortality and has strong anti-phagocytic and competitive traits in vitro. ConclusionsThe high-risk ST11 KL64 CRKP subclone showed strong expansion potential and survival advantages, probably owing to genetic factors.

Amino acid composition of pollen &lt;i&gt;Pinus sylvestris&lt;/i&gt; L. and &lt;i&gt;Pinus sibirica&lt;/i&gt; Du Tour growing in the Baikal region

The purpose of the study was to determine the qualitative and quantitative amino acid composition of pollen Pinus sylvestris L. and Pinus sibirica Du Tour. Pine pollen was collected in June 2021 at natural sites on the southeastern coast of Lake Baikal. The mass fraction of crude protein was determined by the Kjeldahl method; the protein composition and individual amino acid content were determined by high-performance liquid chromatography with post-column modification of ninhydrin on an LA8080 automatic analyser (Hitachi, Japan). The crude protein content of the pollen was 14.38–15.94%. Pine pollen protein is shown to contain 17 amino acids, including 9 essential amino acids: valine, isoleucine, leucine, threonine, methionine, phenylalanine, lysine, histidine, and arginine. The content of the sum of amino acids was 141.4–156.5 mg/g, including essential amino acids 45.9-48.4%. The following amino acids are dominant in Pinus sylvestris and Pinus sibirica pollens (mg/g): monoaminodicarboxylic acids – glutamic (21.3–24.2) and aspartic (13.0–14.2), diaminocarboxylic acid arginine (17.0–17.4) and heterocyclic amino acid proline (14.7–16.2). The obtained results can be useful in the development of drugs and biologically active additives based on pollen Pinus sylvestris and Pinus sibirica, which, due to the presence of the above amino acids, have a nootropic, immunomodulatory, cardiac stimulating, and detoxifying effect.