Sources Soybean Meal Research Articles

Detection of Transgenic and Endogenous Plant DNA Fragments in the Blood, Tissues, and Digesta of Broilers

The aim was to determine the fate of transgenic and endogenous plant DNA fragments in the blood, tissues, and digesta of broilers. Male broiler chicks (n = 24) were allocated at 1 day old to each of four treatment diets designated T1-T4. T1 and T2 contained the near isogenic nongenetically modified (GM) maize grain, whereas T3 and T4 contained GM maize grain [cry1a(b) gene]; T1 and T3 also contained the near isogenic non-GM soybean meal, whereas T2 and T4 contained GM soybean meal (cp4epsps gene). Four days prior to slaughter at 39-42 days old, 50% of the broilers on T2-T4 had the source(s) of GM ingredients replaced by their non-GM counterparts. Detection of specific DNA sequences in feed, tissue, and digesta samples was completed by polymerase chain reaction analysis. Seven primer pairs were used to amplify fragments ( approximately 200 bp) from single copy genes (maize high mobility protein, soya lectin, and transgenes in the GM feeds) and multicopy genes (poultry mitochondrial cytochrome b, maize, and soya rubisco). There was no effect of treatment on the measured growth performance parameters. Except for a single detection of lectin (nontransgenic single copy gene; unsubstantiated) in the extracted DNA from one bursa tissue sample, there was no positive detection of any endogenous or transgenic single copy genes in either blood or tissue DNA samples. However, the multicopy rubisco gene was detected in a proportion of samples from all tissue types (23% of total across all tissues studied) and in low numbers in blood. Feed-derived DNA was found to survive complete degradation up to the large intestine. Transgenic DNA was detected in gizzard digesta but not in intestinal digesta 96 h after the last feeding of treatment diets containing a source of GM maize and/or soybean meal.

Raw and extruded pea (Pisum sativum), faba bean (Vicia faba var. minor) and lupin (Lupinus albus var. multitalia) as alternative protein sources in broiler diets

Research on vegetable-based protein sources has grown as a result of the European Union ban on the inclusion of meat and bone meal in diets of agricultural livestock. This together with recent concern over genetically modified soybeans, the protein source of choice for monogastric diets, has sparked research into the identification of some alternative protein sources. The objective of this study was to evaluate the effect of raw or extruded pea (RP and EP), faba beans (RFb and EFb) or lupin (RL and EL) in partial substitution of soybean meal and other starch sources in broiler diets. A total of 462-d-old male Ross chicks, Marek vaccinated, were randomly assigned to seven dietary treatments (3 pens/treatment). Chicks were floor housed, ad libitum fed isocaloric and isonitrogenous diets and had free access to water. Artificial light was provided 10 h/d. The amount of alternative proteins used as fed basis and for 1-10 d-old and 11-42 d-old growing periods were: RP and EP: 350 g/kg for all diets; RFb: 480 and 500 g/kg; EFb: 240 and 250 g/kg; RL: 360 and 300 g/kg; EL: 180 and 150 g/kg. Feeds were analyzed for tannins, polyphenol, genisteine, and daidzeine contents and the in vitro alpha-amilase starch digestibility was measured. At the end of the trial performance parameters were calculated, animals were processed and percentage yields (one per pen) of breast meat and leg quarters were quantified. The polyphenol concentrations and antitrypsin activity were reduced and the alpha-amylase digestibility increased in EP and EFb. The feed conversion ratio was not significantly affected, however, the feed intake was reduced (P<0.01) in EL diet compared to the control group and to the RL diet. The RP group had lower dressing percentage (P<0.05), whereas groups fed faba bean had higher breast yield (P<0.01) independent of processing. There were no statistical differences in percent yield of the leg quarters. Data support the utilization of these alternative proteins sources into broilers diets as a viable solution to counteract the current constraints of soybean meal. Partial improvement was seen with EFb and EP, which was not seen however for the lupin based diets.

Effects of Soybean Meal Sources and Level on Growth Performance of Weanling Pigs

Three experiments were conducted to compare the effects of increasing solvent-extracted soybean meal (SBM) or extruded-expelled SBM (EESOY) in diets for early weaned pigs. Pigs were fed a control diet (no SBM) or diets containing 20% or 40% SBM or EESOY. In Exp. 1, diets were formulated with calculated values for SBM and EESOY. In Exp. 1, from d 0 to 14, no differences were observed in ADG or ADFI (P>0.05), but gain-to-feed ratio (G:F) decreased (linear, P<0.03) with increases of either source. We speculated that the differences in performance resulted from the low protein content (lysine) of the EESOY compared with SBM (43.6% vs 46.5%). In Exp. 2, SBM nutrient values were analyzed prior to the experiment and used in diet formulation. From d 0 to 14, increasing SBM decreased (linear, P<0.02) ADFI and G:F. Increasing EESOY decreased ADG, ADFI, and G:F (linear, P<0.01). The EESOY from both experiments had trypsin inhibitor (TI) values >8 mg TI/g, suggesting under processing, whereas SBM only contained <2 mg TI/g. In Exp. 3, EESOY and SBM were analyzed for TI and CP; then, these values were used in diet formulation. From d 0 to 14, increasing EESOY decreased (linear, P<0.01) ADG and ADFI and improved (linear, P<0.01) G:F. Increasing SBM decreased ADFI, but improved G:F (linear, P<0.02). No differences (P>0.05) were found between SBM sources. These results suggest that when processed properly, EESOY has similar nutritional value as SBM in nursery pig diets.

Chemical and Nutritional Characteristics of United States Soybeans and Soybean Meals

Two experiments were conducted to determine U.S. soybean meal (SBM) variation. In experiment 1, SBM from 55 U.S. processors was evaluated. Significant (P < 0.05) but numerically small differences were detected in dry matter (DM) and organic matter (OM) concentrations. Crude protein (CP) concentrations (51.6-54.6%) were higher (P < 0.05) in SBM produced in the southern U.S. Lipid and total dietary fiber concentrations also differed (P < 0.05). The protein dispersibility index was affected (P < 0.05) by the source of SBM. In experiment 2, soybeans and resultant SBMs were obtained from 10 U.S. processing plants. Soybean DM, OM, and CP concentrations differed (P < 0.05). Soybean meal varied (P < 0.05) in CP (48.2-56.2%), acid-hydrolyzed fat (3.3-9.2%), total dietary fiber (17.0-20.7%), and lysine concentrations. Soybean meal carbohydrate composition was also affected by processing conditions. These results indicate a significant variation in chemical and nutritional characteristics of U.S. SBM from different sources.

Evaluating processing temperature and feeding value of extruded-expelled soybean meal on nursery and finishing pig growth performance.

We conducted two experiments comparing the use of extruded-expelled soybean meal (EESoy) to solvent-extracted soybean meal (SBM) in swine diets. In Exp. 1, the objective was to determine the optimal processing temperature of EESoy for nursery pig growth performance. Pigs (n = 330, 13.2 +/- 2.3 kg of BW) were fed a control diet containing SBM with added fat or one of five diets containing EESoy extruded at 143.3, 148.9, 154.4, 160.0, or 165.6 degrees C. All diets were formulated on an equal apparent digestible lysine:ME ratio. From d 0 to 20, no differences were observed (P > 0.32) in ADG or ADFI (average of 544 and 924 g/d, respectively). However, gain:feed ratio (G/F) improved (quadratic, P < 0.01, range of 0.56 to 0.60) with increasing processing temperature, with the greatest improvement at 148.9 degrees C. In Exp. 2, the objective was to determine the feeding value of EESoy relative to SBM with or without added fat for growing-finishing pigs in a commercial production facility. A total of 1,200 gilts (initially 24.5 +/- 5.1 kg of BW) was used, with 25 pigs per pen and eight replications per treatment. Dietary treatments were arranged in a 2 x 3 factorial, with two sources of soybean meal (SBM or EESoy) and three levels of added fat. Pigs were phase-fed four diets over the experimental period and added fat (choice white grease) levels were 0, 3.4, and 7% initially, with the added fat levels decreasing in the next three dietary phases. Energy levels were based such that the higher energy in EESoy (with or without added fat) was calculated to be equal to that provided by SBM with added fat. From 24.5 to 61.2 kg, pigs fed EESoy had greater (P < 0.07) G/F than those fed SBM. Increasing added fat in either EESoy- or SBM-based diets increased G/F (linear, P < 0.0003). From 61.2 to 122.5 kg, ADG and G/F were unaffected in pigs fed EESoy and/or increasing added fat (P > 0.10). For the overall growing-finishing period, ADG was unaffected (P > 0.61) by increasing energy density of the diet; however, ADFI decreased (P < 0.05) and G/F increased (P < 0.02, range of 0.37 to 0.40) as energy density increased with either EESoy or added fat. Carcass leanness was not affected by dietary treatment. These results indicate that EESoy should be extruded at 148.9 to 154.4 degrees C, and that increasing dietary energy density by using EESoy and/or added fat improves feed efficiency in finishing pigs reared in a commercial environment.

Effect of soybean meal particle size on amino acid and energy digestibility in grower-finisher swine.

A study was conducted using the ileal digestibility technique with grower-finisher pigs to evaluate the effects of particle size reduction of soybean meal (SBM) on amino acid and energy digestibility. Soybean meal was processed through a hammer mill to achieve average particle sizes of 900, 600, 300, and 150 microm. The treatments included the use of two soybean meal sources and soy protein concentrate. One source of SBM was ground to four different particle sizes (i.e., 949, 600, 389, 185); a second source was a common SBM source used in other trials (i.e., 800 microm). The soy protein concentrate had an average particle size of 385 microm. A low-protein (5% casein) diet was fed to determine endogenous amino acid losses. This experiment was conducted in a 7 x 7 Latin Square design in two replicates using 14 crossbred barrows ([Landrace x Yorkshire] x Duroc) that averaged 28 kg BW and 60 d of age. Animals were surgically fitted with a T-cannula at the distal ileum. Treatment diets were fed in meal form, initially at 0.09 kg BW(0.75) and at graded increases at each subsequent period. Pigs within replicate were fed a constant quantity of their treatment diet for a 5-d adjustment period followed by a 2-d collection of ileal digesta samples. Apparent and true digestibility of amino acids was calculated by use of chromic oxide (0.5%) as an indigestible marker. Apparent digestibility of isoleucine, methionine, phenylalanine, and valine increased linearly (P < 0.05) as particle size decreased. True digestibility of isoleucine, methionine, phenylalanine, and valine increased linearly (P < 0.05) as particle size decreased. When the essential amino acids were averaged, apparent digestibility increased (P < 0.10) from 83.5% to 84.9% as particle size decreased, whereas, nonessential amino acid digestibility increased only slightly (P > 0.15). Essential amino acid true digestibility increased numerically from 91.0% to 92.4% as particle size decreased. Energy digestibility was not affected by particle size (P > 0.15). These results suggest that a reduction in particle size of soybean meal resulted in a small increase in the digestibility of its amino acids with the essential amino acids being affected more than the nonessential amino acids. The largest improvement in digestibility, however, was obtained when the particle size was reduced to 600 microm.

Replacing soybean meal with sunflower meal with or without fibrolytic enzymes in fattening diets of goat kids

Nutritional value of sunflower meal (SFM) as a replacement protein source of soybean meal (SBM) for goat kids was evaluated. Thirty-six weaned Shami goat kids were randomly selected and used in a complete randomize design (CRD) trial with three treatments. Kids were divided into three groups of equal number and allocated into pens of similar size and condition. For each group, kids were fed one of three diets containing SBM, SFM, or SFM supplemented with fibrolytic cellulases enzyme (sunflower meal with enzyme, SFME). The experiment lasted 12 weeks. Total gain and average daily gain were higher ( P<0.05) for kids fed SFME with no differences obtained between SBM and SFM. Daily gain values averaged 221.7, 155.8, and 141.6 g for the three experiments, respectively. SFME group had the highest ( P<0.05) feed intake value. At the same time, SFM and SBM group were not different ( P>0.05) with higher value for the former. Same results were obtained for the feed conversion ratio (FCR) where SFME kids had the highest ( P<0.05) FCR value of 4.7. No difference was observed for SBM in feed conversion ratio value (6.6) compared to that of the SFM (7.59). It is concluded that sunflower meal can successfully replace soybean meal as a protein source in fattening diets. Moreover, fibrolytic enzymes can be effectively used to enhance the feeding value of the SFM and overcome its high fiber content.

Effects of extruded-expelled soybean meal and solvent extracted soybean meal level of growth performance of weanling pigs (2003)

Summary A total of 350 weanling pigs (initially 15.7 lb) were used to evaluate the effects of soybean meal source and level on growth performance of early weaned pigs. Dietary treatments included a control diet containing no soybean meal, or diets containing 20% or 40% of either solvent extracted soybean meal (SBM) or extruded-expelled soybean meal (EESOY). The SBM and EESOY were analyzed for trypsin inhibitor (0.7 mg TI/g and 1.8 mg TI/g, respectively) to ensure quality, and actual crude protein values (46.9% and 48.3% as-fed, respectively) were used in diet formulation. From d 0 to 14, increasing EESOY decreased ADG and ADFI (linear, P 0.05) were found between soybean meal sources throughout the trial. The results of this study suggest extrudedexpelled soybean meal processed properly and fed in diets immediately after weaning did not improve growth performance of nursery pigs relative to conventional solvent extracted soybean meal. When EESOY or SBM was included at 40% in diets fed immediately after weaning, growth performance of weanling pigs was poorer than if fed at lower levels (20%). Feeding properly processed EESOY resulted in similar growth performance compared to feeding SBM.

Desempenho de Novilhos Mestiços na Fase de Crescimento Suplementados Durante a Época Seca

Objetivou-se avaliar o desempenho de novilhos em recria recebendo, durante o período da seca, suplementos constituídos por diferentes fontes protéicas. Os tratamentos empregados foram: sal mineral (MM) e suplementos constituídos pelas fontes protéicas farelo de soja (FS), farelo de algodão (FA), farelo de glúten de milho (FG) e farelo de trigo (FT). Os suplementos foram fornecidos ad libitum, empregando-se uréia e cloreto de sódio como controladores de consumo, ajustados para permitir a ingestão de 1 kg suplemento/dia. O nível médio de uréia empregado foi de 10%, com exceção do FT (8%), o qual parece agir como adjuvante sobre o controle de ingestão. Foram utilizados 35 novilhos mestiços, castrados, com peso médio inicial de 248 kg, distribuídos entre tratamentos e alocados em cinco piquetes (8 ha) de Brachiaria decumbens. O consumo médio de suplementos foi de 0,046; 1,48; 1,49; 1,50; e 1,48 kg/animal/dia para os tratamentos MM, FS, FA, FG e FT, respectivamente. Os valores superiores a 1 kg/dia foram justificados em virtude da elevação de peso dos animais. O ganho médio diário para MM foi de 0,09 kg/dia, sendo inferior aos tratamentos com suplementação, os quais não diferiram entre si, apresentando média de 0,47 kg/dia.

Comparative study of three expeller process soybean meals in diets for lactating dairy cows (2002)

Forty-eight primiparous Holstein cows were used in two simultaneous 3x3 Latin squares with 28-day periods to evaluate expeller soybean meal from three sources. Six pens containing eight cows each were utilized. Cows were pen fed diets formulated in accordance with NRC (2001) recommendations and based on the assumption that the three sources of soybean meal were nutritionally identical. Diets contained on a dry matter basis, 24.3% chopped alfalfa hay, 9.3% field processed corn silage, 9.2% whole fuzzy cottonseed, 19.2% wet corn gluten feed (Minnesota Corn Processors, Inc., Columbus, NE), and 38% grain mix (70.2% dry rolled corn grain, 18.44% expeller soybean meal, 2.15% wet molasses, 3.47% Menhaden fish meal, and 5.74% min/vit premix). The sources of expeller soybean meal were Grain States Soya, Inc., West Point, NE; NCKP, LLC., Washington, KS; and Bruning Grain and Feed, Bruning, NE. All cows averaged 77 lbs. of milk and consumed approximately 55 lbs. of dry matter daily. Dry matter intake averaged 3.9% of body weight and their production efficiency (ECM/DMI) was 1.45. No differences due; Dairy Day, 2002, Kansas State University, Manhattan, KS, 2002

Effects of soybean meal source and level on growth performance of weanling pigs (2002)

A total of 525 weanling pigs (initially 13.0 lb) were used in two experiments to evaluate the effects of soybean meal source and level on growth performance of early weaned pigs. In both experiments, dietary treatments included a control diet containing no soybean meal, or diets containing 20% or 40% of either solvent extracted soybean meal (SBM) or extruded-expelled soybean meal (EESoy). In Exp. 1, diets were formulated with NRC (1998) nutrient values for the solvent extracted soybean meal and previously determined values (1998 KSU Swine Day Report of Progress) for the extruded-expelled soybean meal. In Exp. 1, from d 0 to 7, increasing solvent extracted soybean meal or extrudedexpelled soybean meal decreased (linear, P<0.05) ADG. Feed efficiency was reduced with an increase of either soybean meal source (SBM quadratic, P<0.05; EESoy linear, P<0.05). However, the mean ADG and F/G of pigs fed solvent extracted soybean meal were better than the mean of pigs fed extruded-expelled soybean meal. No differences were found in growth performance from d 7 to 14 and 14 to 21. However, from d 0 to 14, F/G became poorer (linear, P<0.06) as either soybean meal source increased, and the mean F/G of pigs fed solvent extracted soybean meal was better than those fed extrudedexpelled soybean meal. For the overall growth period, d 0 to 21, F/G became poorer (linear, P<0.04) as solvent extracted soybean meal increased. After the trial was completed, the soybean meal sources were chemically analyzed and the extruded-expelled soybean meal was found to be lower in crude protein (43.6% vs 46.5%) than what was used in diet formulation. We speculated that the differences in growth performance between the two soybean meal sources could have been a result of the low protein (lysine) concentrations. Therefore, in Exp. 2 diets were formulated with actual analyzed nutrient soybean meal values.

Effects of Three Different Soybean Meal Sources on Layer and Broiler Performance

Soybean meal (SBM) is a major protein source in poultry feeds and one of the best quality ingredients because of the relatively high protein content, good amino acid profile and bioavailability. But soybean meal quality is largely dependent on the processing technology and origins. In this experiment, effects of three different soybean meals were evaluated in layer (experiment 1) and broiler (experiment 2). Soybean meal sources used in the experiments were the US-originated dehulled soybean meal (USDHSBM), India-originated non-dehulled soybean meal (India SBM) and Brazil-originated non-dehulled soybean meal (Brazil SBM). Experiment 1 was conducted during growing and laying periods and evaluated the interactive effects of soybean meal sources according to feeding periods on growth performance and egg quality. Experiment 2 was conducted during growing period (day 1-35) and finishing period (day 35-42). The growth performance was measured for the same periods and any possible interaction between soybean meal origins and crude protein levels was also studied. In experiment 1, chicks fed India SBM utilized feed more efficiently (p<0.05) than those fed Brazil SBM from day 29 to day 42. The body weights of layers during the laying period had no relation to egg production. But egg weights were significantly heavier in all the USDHSBM fed groups than other groups (p<0.001) and depended on feed protein source during growing period (p<0.001). The average egg weight of the USDHSBM fed group scored the highest value (65.4 g), followed by the Brazil SBM fed group (62.1 g) and India SBM fed group (62.1 g). There was an effect of interaction between origins of soybean meal fed group in growing and laying period on eggshell color (p<0.01). Eggshell was significantly stronger in the USDHSBM fed (for growing period) groups than other groups (p<0.05) on 31 s t week. Haugh's unit (HU), albumin index and yolk index of the USDHSBM fed group in growing stage were significantly superior (p<0.001) to other groups. In experiment 2, for the 7-week, chicks on the India SBM group gained less (p<0.001) weight than other groups. While daily gain of India SBM chicks was not affected by dietary crude protein level, those of the USDHSBM and Brazil SBM chicks were linearly increased as dietary crude protein level increased from 18% to 20%. The gain per feed ratio of the USDHSBM group was the highest (0.585), followed by the Brazil SBM group (0.568) and India SBM group (0.550) (p<0.01). Therefore, in this experiment, the use of USDHSBM with excellent protein quality and amino acid digestibility could be of advantage to the economic production of layer and broiler.

Effects of Rumen Undegradable Protein and Minerals Proteinate on Early Lactation Performance and Ovarian Functions of Dairy Cows in the Tropics

A 90 d study was designed to investigate the effects of rumen undegradable protein (RUP) and a mixture of Cu, Zn and Mn proteinate (CZMP) on milk yield and composition and ovarian functions during rainy months. Twenty four Holstein× indigenous cows in their 2nd and 3rd lactation were randomly allocated to total mixed rations (TMR) containing soy bean meal (SBM) as a source of rumen degradable protein (RDP), SBM plus CZMP, and formalin treated SBM (FSBM) as a source of RUP. Maximum and minimum temperature humidity index during the experimental period were 83.6-84.7 and 75.4-76.1. There were no differences (p>0.05) in intakes of dry matter, crude protein and net energy and in contents of butterfat, lactose and minerals. Cows on TMR containing FSBM not only lost less weight (-278, -467 and -433 g/d) with more intake of RUP (0.92, 0.58 and 0.59 kg/d) but also produced more milk (19.27, 18.23 and 18.13 kg/d) and 4% fat corrected milk (18.57, 17.57 and 17.51 kg/d) with more protein (3.06, 2.81 and 2.80%), solids-not-fat (8.69, 8.38 and 8.38%) and less milk urea N (9.3, 15.4 and 15.0 mg/dl) compared with those on TMR containing SBM and SBM+CZMP, respectively (p 0.05). Whereas incidence of cystic ovaries at 20 and 90 d pospartum was less (p<0.01) in cows on TMR containing SBM+CZMP (37.3 and 12.5%) than those on TMR containing SBM (62.5 and 25%), it was nil for cows on TMR containing FSBM. Cows in all three group differed (p<0.01) from each other for the recurrence of first observed estrus with those on TMR containing FSBM having least days (22, 36 and 47 d) compared with their counterpart on TMR containing SBM+CZMP and SBM, repectively. The results suggest that RUP is one of the limiting factors affecting milk yield and its composition and ovarian functions during early lactation of dairy cows in the tropics. (Asian-Aust. J. Anim. Sci. 2002. Vol 15, No. 6 : 806-811)

Effects of level of supplemental phytase on ileal digestibility of amino acids, calcium, and phosphorus in dehulled soybean meal for growing pigs.

Ileally cannulated pigs were used to assess the effects of four dietary levels of microbial phytase (Natuphos) on the apparent and true digestibility of Ca, P, CP, and AA in dehulled soybean meal. Fourteen pigs (25 kg initial BW) were surgically fitted with T-cannulas at the terminal ileum and assigned to diets in a replicated 7 x 7 Latin square design. Following a 14-d recovery, four diets consisting of 30.5% soybean meal with 0, 500, 1,000, or 1,500 units of phytase/kg of diet were fed. Diets 5 (1.05% lysine, 0.90% Ca, and 0.75% P) and 6 (1.05% lysine, 0.90% Ca, and 0.75% P) contained 35.25% soybean meal and 27.0% soy protein concentrate, respectively. Diet 7 (0.37% lysine, 0.03% Ca, and 0.05% P) was a low-CP, casein-based diet used to estimate the nonspecific endogenous losses of Ca, P, CP, and AA in order to estimate the true digestibility of these nutrients. All diets contained cornstarch and dextrose and were fortified with vitamins and minerals. Chromic oxide was used as an indigestible indicator. The diets were fed daily at 9% of metabolic BW (BW0.75). Apparent and true ileal digestibility of P increased quadratically (P < 0.01) and true digestibility of Ca increased linearly (P < 0.07) with increasing levels of phytase. Apparent digestibility of Ca was unaffected (P = 0.15) by phytase level. Apparent and true ileal digestibility of CP and most AA increased slightly with the addition of 500 units of phytase/kg of diet, but not at higher levels of phytase supplementation (in most cases, cubic effect, P < 0.05). Apparent and true ileal nutrient digestibility coefficients were unaffected by soybean meal source (Diet 1 vs Diet 5), except for arginine and Ca. The apparent and true digestibility coefficients for most of the AA tended (P < 0.10) to be lower in diets containing soy protein concentrate vs the common source of soybean meal used in Diet 5, but ileal digestibilities of Ca and P were unaffected (P = 0.15). In this study, supplemental microbial phytase did not improve the utilization of AA provided by soybean meal but was an effective means of improving Ca and P utilization by growing swine fed soybean meal-based diets.

Effect of Various Soybean Meal Sources and Avizyme on Chick Growth Performance and Ileal Digestible Energy

Although it is well accepted that soybean meal (SBM) is a high-quality feed ingredient for poultry, the digestibility of the dry matter and energy in SBM are quite low. One possible means for improving the digestibility of SBM is the use of enzymes.Twelve SBM, obtained from commercial processing plants in the U. S. and other countries, were evaluated with and without the addition of Avizyme in four 2-wk broiler chick assays. The analyzed values (%) of selected nutrients (mean and range) in the SBM were: CP, 45 (40 to 49); dry matter, 88 (87 to 89); lysine, 2.9 (2.57 to 3.27); methionine, 0.69 (0.60 to 0.75); cystine, 0.64 (0.60 to 0.76). KOH protein solubility (%) in the SBM (mean and range) was 77 (71 to 83). Growth performance and ileal digestible energy (DE) for chicks were affected by SBM source (P<.01). Addition of Avizyme had no effect (P>.10) on chick growth performance but significantly increased ileal DE (P<.01). There was a significant interaction (P<.01) between SBM source and Avizyme for ileal DE, indicating that Avizyme increased ileal DE more in some SBM than in others. Results of this study indicate that chick growth performance was greatly influenced by SBM source and that both SBM source and Avizyme affected ileal DE.

Characterizing the feeding value of extruded-expelled soybean meal with or without added fat in a commercial swine production facility (2000)

A total of 1,200 gilts was used to evaluate the effects of replacing conventionally processed soybean meal with extruded-expelled soybean meal on finishing pig growth performance. Dietary treatments were arranged in a 2 × 3 factorial with two sources of soybean meal (solvent-extracted or extruded-expelled) and three levels of added fat (none, 3.4, and 7% in Phase 1 than decreasing in subsequent phases). Energy levels were adjusted such that the higher energy in extruded-expelled soybean meal (with or without added fat) was equal to that provided by solvent-extracted soybean meal with added fat. From 54 to 135 lb, pigs fed extruded-expelled soybean meal had improved ADG and F/G compared to those fed solvent-extracted soybean meal. Increasing added fat in either extruded-expelled- or solvent-extracted soybean meal-based diets linearly improved ADG and F/G. From 135 to 270 lb, pigs fed extruded-expelled soybean meal and(or) increasing added fat had decreased feed intake. For the overall growing-finishing period, ADG was unaffected by increasing energy density. However, ADFI was decreased and F/G improved as energy density of the diet was increased either with extruded-expelled soybean meal and(or) added fat. Carcass leanness was not affected by dietary treatment. These results indicate that increasing the energy density of the diet by using extruded-expelled soybean meal and(or) added fat improves feed efficiency in finishing pigs reared in a commercial environment.; Swine Day, Manhattan, KS, November 16, 2000

Variability among sources and laboratories in nutrient analyses of corn and soybean meal. NCR-42 Committee on Swine Nutrition. North Central Regional-42.

A cooperative research study involving members of a regional committee (North Central Regional Committee on Swine Nutrition [NCR-42]) was conducted to assess the variability in nutrient composition (DM, CP, Ca, P, Se, and amino acids) of corn and soybean meal from 16 sources (15 states, mostly in the Midwest) and to assess the analytical variability in nutrient assays among 22 laboratories (labs; 16 experiment station labs and six commercial labs). Corn samples were obtained from each participating station's feed mill during a 3-yr period (1989, 1990, and 1992), as were soybean meal samples during a 2-yr period (1989 and 1990). Both regular soybean meal (with hulls) and dehulled soybean meal were represented in the study. Samples were analyzed for DM and CP by all 22 labs, for Ca and P by 15 labs, for amino acids by 10 labs, and for Se by 6 labs. Each lab used its own analytical procedures. Samples of corn and soybean meal varied in their nutrient composition depending on the area of origin. The greatest variation among sources was in Se concentration, ranging from .02 to .29 mg/kg in corn and .08 to .95 mg/kg in soybean meal. Crude protein and lysine in corn were positively correlated, but the regression coefficient was low (r2 = .49). The relationship between CP and lysine for the two soybean meals combined was considerably stronger (r2 = .81). Lysine in corn increased by .018 percentage point and lysine in soybean meal (regular and dehulled combined) increased by .063 percentage point for each 1 percentage point increase in CP. Except for CP and Se, the analytical variability among labs was as great as, and in some cases greater than, the variability in nutrient composition among sources of corn and soybean meal. Within-lab analytical variability tended to be less than among-lab variability. Some labs performed certain analyses with considerably less variability and more accuracy than others.

Effect of Processed Grain Sorghum and Expeller Soybean Meal on Performance of Lactating Cows

Forty-four Holstein cows were used to measure milk production responses to dry-rolled versus pelleted grain sorghum and expeller versus solvent soybean meal in a 2 × 2 factorial arrangement of treatments. Pelleted grain sorghum was processed by grinding, increasing moisture to 31%, extruding, and drying to an exit temperature of 93°C. Grain sorghum was included in the diets at 27% of dry matter, and soybean meal was the primary source of supplemental protein. Few interactions were observed between the methods of processing grain sorghum and sources of soybean meal. Pelleting of grain sorghum decreased dry matter intake 5%, but increased milk production 3%, protein yield 4%, and efficiency 7%; fat yield was unaffected. Replacement of solvent soybean meal with expeller soybean meal had little effect on intake, but increased milk production 3%, fat yield 5%, and efficiency 4%; protein yield was unaffected. Plasma concentrations of amino acids (AA) were increased by pelleted grain sorghum and by expeller soybean meal at 5 and 10 wk after initiation of treatment, indicating that both of these treatments increased the supply of AA to the intestine. Pelleting improved the nutritive value of grain sorghum for lactating cows. Although total milk production and fat yield increased in response to expeller soybean meal, the lack of response in protein yield to this increased supply of RUP indicated that the effect was not solely due to an increased supply of AA to the intestine.

Nutritional value for swine of soybean meal produced by isopropyl alcohol extraction.

Three experiments were conducted to compare the nutritional value of soybean meal produced by extraction with 95% isopropyl alcohol (IPA) or hexane (HEX) for swine. The same batch of soybeans and the same processing equipment were used to produce both soybean meals. Analyzed contents of the IPA and HEX meals were, respectively: CP, 48.7, 47.0%; lysine, 3.11, 3.06%; urease, .24, .13 delta pH. In Exp. 1, two soybean meals and dietary lysine at .90 or 1.25% were used in a 2 x 2 factorial arrangement of treatments. Corn-based diets were fed to 32-d-old pigs for 26 d. There were no dietary lysine x soybean meal interactions (P > or = .35). Increasing dietary lysine increased (P < .001) ADG and gain/ feed, but soybean meal source did not affect performance. In Exp. 2, the nutritional value of HEX and IPA meals were evaluated in a N balance study using 34-kg barrows and isonitrogenous corn-based diets containing equal N from either HEX or IPA. Apparent total tract N and DM digestibility were similar for both diets. Nitrogen retention (14.4 vs 13.7 g/d, P < .10) and apparent biological value (56.5 vs 54.5%, P < .05) were slightly higher for HEX than for IPA. The effect of feeding HEX and IPA meals on morphological changes of small intestine in pigs weaned at 21 d of age was investigated in the last experiment. At 28 d of age, weaned pigs that were fed diets containing either HEX or IPA and unweaned control pigs were killed for the examination. Villus height and lamina propria depth at the duodenum were similar among all treatments. At the jejunum, weaned pigs had smaller (P < .05) villus height and greater lamina propria depths (P < .001) than unweaned pigs. Dietary soybean meal source did not affect villus height, but lamina propria depth was less (P < .10) for pigs fed IPA. The results of these experiments indicate that soybean meals produced using IPA or HEX as the solvent have equal nutritional value for swine.

Comparative evaluation of different soybean meal and the replacement using peanut, rapeseed and fish meal for broilers .

Two feeding experiments have been conducted to test the different sources of soybean meal (local, India, USA) and the partial substitution with peanut meal (10%), rapeseed meal (5%) and fish meal (5%) in the diet for broiler. The first feeding experiment was carried out on broiler starter for 4 weeks in wire cages . Twelve rations in factorial design (3 x 4) were conducted using 6 birds/cage and replicated 6 times . Birds fed India soybean meal had significantly less body weight (1,000 g) than those fed local or USA soybean meals (1,037 and 1,023 g, respectively) . Partial substitution with peanut, rapeseed or fish meal did not affect body weight, feed consumption or feed/gain ratio . The average consumption and feed/gain were 1,535 g and 1,569, respectively . In second experiment, one thousand five hundred broiler chicks were allocated in 6 dietary treatments in factorial design (3 x 2) . Factor one was different sources of soybean meal (local, India and USA) and factor two was the inclusion of peanut meal at 0 and 10%. Birds were kept on litter system for 6 weeks . Each treatment used 5 replicates and 50 birds/replicate . The results show no effect of the treatment to any parameter measured . Body weight of bird fed local, India and USA soybean meal were 1,662, 1,641 and 1,669 g and feed/gain were 2 .043, 2 .051 and 2.035, respectively . Cost analysis indicates that ration uses local soybean meal gives a higher cost than those of India and USA soybean meals. Key words : Soybean meal, peanut meal, rapeseed meal, fish meal, broiler