Sonication Cycles Research Articles

Evidence for homology of buffalo and goat granular cationic peptides

Due to lack of studies on PMN granular cationic proteins to assess immunostatus of buffaloes, the peripheral PMN leukocytes were subjected to homogenization by sonication and centrifugation cycles in order to obtain pure pellet of granules. Acid extracted granules contained cationic proteins and peptides, which were further separated by exclusion Sephadex G-50 chromatography, the peptides were in the last peak and were found to be active against Gram-negative ( Escherichia coli) and Gram-positive ( Staphylococcus aureus) bacteria. The peptides were conjugated to ovalbumin in order to develop antibodies, which were further tested by Ouchterlony double immunodiffusion and enzyme linked immunosorbent assay. These tests demonstrated the antigenic relationship between buffalo and goats for the PMN cationic peptides as well as the peptide release which was also monitored in activated buffalo PMN leukocytes.