Somatic Nerve Research Articles

Olf413 an octopamine biogenesis pathway gene is required for axon growth and pathfinding during embryonic nervous system development in Drosophila melanogaster

ObjectiveNeurotransmitters have been extensively studied as neural communication molecules. Genetic associations discovered, and indirect intervention studies in Humans and mammals have led to a general proposition that neurotransmitters have a role in structuring of neuronal network during development. olf413 is a Drosophila gene annotated as coding for dopamine beta-monooxygenase enzyme with a predicted function in octopaminergic pathway. The biological function of this gene is very little worked out. In this study we investigate the requirement of olf413 gene function for octopamine biogenesis and developmental patterning of embryonic nervous system.ResultIn our study we have used the newly characterized neuronal specific allele olf413SG1.1, and the gene disruption strain olf413MI02014 to dissect out the function of olf413. olf413 has an enhancer activity as depicted by reporter GFP expression, in the embryonic ventral nerve cord, peripheral nervous system and the somatic muscle bundles. Homozygous loss of function mutants show reduced levels of octopamine, and this finding supports the proposed function of the gene in octopamine biogenesis. Further, loss of function of olf413 causes embryonic lethality. FasII staining of these embryos reveal a range of phenotypes in the central and peripheral motor nerves, featuring axonal growth, pathfinding, branching and misrouting defects. Our findings are important as they implicate a key functional requirement of this gene in precise axonal patterning events, a novel developmental role imparted for an octopamine biosynthesis pathway gene in structuring of embryonic nervous system.

Role of Uttarabasti in Neurogenic Urinary Incontinence

Neurogenic bladder refers to dysfunction of the urinary bladder arising from internal or external trauma, disease, or injury of the central, autonomic or somatic nervous system in the control of micturition. Depending on the nerves involved and the nature of the damage, bladder becomes either overactive or underactive and this usually presents clinically as urinary incontinence or retention of urine. Urinary Incontinence (UI) is a hygienic problem and is one of the troublesome, important cause of morbidity and impairment of quality of life, in both men and women. It may lead to recurrent UTI and ascending infections which affect the functioning of kidney. Deranged function of Vatadosha especially Apanavata along with Pranavata and Vyanavata are the main culprits of neurogenic bladder. Vitiated Doshas cause Mootravahasrotodushti and Atipravritti of mootra. This case study discusses the effect of Mootramarga uttarabasti in reducing the signs and symptoms of neurogenic urinary incontinence and thereby improving the quality of life. A 66 year old male patient was admitted with weakness in left upper and lower limbs, slurred speech, and difficulty in controlling micturition since 5 months. He was a diagnosed case of stroke on the basis of a brain computed tomography scan. During the course of management, Uttarabasti was administered for 8 days with Dhanwantarataila and Balamoolakashaya alternatively. The intervention resulted in reducing the symptoms of neurogenic urinary incontinence as evidenced by the reduction in ICIQ-UI -SF score. It was observed that the overall impact of the disease was reduced and quality of life improved with the help of King’s Health Questionnaire (KHQ).

Features of the influence of emotional state on the implementation of arbitrary purposeful bimanual movements

The emotional state has a significant impact on the autonomic and somatic nervous systems, which give various manifestations. All external manifestations of emotions are strictly determined and begin to be realized when the cortical areas are activated. The leading role in the realization of the emotional state belongs to the frontal lobes of the cortex and the limbic system. The precentral sulcus is the cortical center of complex purposeful movements. The purpose of this work is to study the influence of the emotional state on the implementation of the motor program of bimanual purposeful voluntary movement. The emotional state was determined using the K scale. Izard. The bimanual motor activity of the hands was measured using the method of supportmetry. As a result of the study, it was found that a negative emotional state leads to a decrease in the reaction rate of the reaction to an error, which may be caused by a change in the initial alignment between the motor cortex and the limbic system.

EBV-Associated Smooth Muscle Tumors With Autoimmune Hemolytic Anemia and Hepatitis B Infection: Report of a Previously Undescribed Neoplasm With Review.

Epstein-Barr virus-associated smooth muscle tumor (EBV-SMT) is rare in adults. The presence of intratumoral T lymphocytes and primitive rounded cells characterized this neoplasm. We report a 24-year-old Chinese man who developed EBV-SMT in the right adrenal gland with hepatitis B infection and autoimmune hemolytic anemia without a history of HIV infection, primary immune deficiency, organ transplantation, or malignant tumor. This patient had an unknown immunodeficient state. EBV-SMTs are commonly located in the liver, lung, and gastrointestinal tract but rarely in the adrenal gland. We reviewed 10 reported literature on EBV-SMT in the adrenal gland. It is imperative to distinguish EBV-SMT from conventional somatic smooth muscle tumors. The discovery of EBV-SMT forces the clinician to conduct a thorough evaluation of immune function and immune status surveillance, and these patients are vulnerable to subsequent malignant tumors.

Surgical Anatomy of the Clitoris and Surrounding Vulvar Structures

(Abstracted from Am J Obstet Gynecol 2023;229(4):460–462) During periurethral and vulvar procedures, thorough knowledge of clitoral anatomy is imperative to ensure the preservation of the autonomic and somatic nerves. This reduces chances of postoperative clitoral pain, sexual dysfunction, and decreased sensation, as entrapment and compression of these nerves during surgical procedures have been observed to likely cause such dysfunction.

Gender and age-related features of anxiety and depressive symptoms in patients in the acute phase of COVID-19.

Aim: To investigate the gender and age characteristics of psychopathological symptoms in patients in the acute phase of respiratory infection SARS-CoV-2. Materials and Methods: A total of 66 patients aged 8 to 92 years, sick with COVID-19 during inpatient treatment with lung damage from 30 to 70%, confirmed radiologically, in the period up to 20 days from the onset of the disease, were examined. To assess the mental status of patients with COVID-19, we used a clinical, clinical-psychopathological, psychometric method, which included the following questionnaires as part of psychiatric screening for studying psychopathological symptoms upon admission to the hospital. The Patient Health Questionnaire - PHQ-9, The Generalised Anxiety Disorder - GAD-7, Hamilton Test were used. The research data collection period is from 2020 to 2022. Results: Acute respiratory infection СОVID-19 affects the psycho-emotional state. During inpatient treatment, 60.6% of patients have symptoms of depression, 66.1% have anxiety symptoms. Most women with COVID-19 are characterized by moderate depression according to PHQ-9, mild anxiety according to GAD-7, and moderate anxiety according to Hamilton. Mild depression characterizes most men according to PHQ-9, absence of anxiety according to GAD-7, and mild anxiety according to Hamilton. Anxiety symptoms were less pronounced among men with COVID-19, with insomnia and respiratory symptoms being the most significant. Among women, anxiety (p≤0.001), tension, depression (p≤0.001), somatic muscle (p≤0.001) and respiratory symptoms (p≤0.001) predominate in the structure of anxiety during the active phase of COVID-19. Conclusions: The data obtained will be useful in the development of psychoprophylactic measures in patients with COVID-19.

PHYSIOLOGY OF AFFERENT AND CENTRAL PARTS OF AUTONOMIC NERVOUS SYSTEM

The Autonomic Nervous System (ANS) and the endocrine system control the internal environment of the body. The organization of the ANS is on the basis of the reflex arc. Changes in chemical composition, blood pressure, osmotic pressure, stretch in viscera, and temperature are detected by autonomic receptors. The visceral afferent fibres are myelinated and they accompany the visceral motor fibres. The sensory autonomic neurons are pseudo-unipolar cells located in the dorsal root ganglia of somatic spinal nerves. Once the afferent fibres gain entrance to the spinal cord or the brainstem, they are thought to travel alongside the somatic afferent fibres to the autonomic centres in different parts of the brain. Information reaches the higher autonomic centres from viscera through an ascending system that involves the nucleus tractus solitarius, the parabrachial nucleus, the periaqueductal gray matter, and the hypothalamus. The hypothalamic paraventricular nucleus, pontine A5 cell group, rostral ventrolateral medulla, and medullary raphe nuclei send direct output to the preganglionic autonomic neurons. The amygdala, mesencephalic periaqueductal gray, caudal ventrolateral medulla, nucleus of the tractus solitarius, and medullary lateral tegmental field feed into these direct pathways. Pak J Physiol 2023;19(4):1-2

Abnormal wiring of the structural connectome in adults with ADHD.

Current knowledge of white matter changes in large-scale brain networks in adult attention-deficit/hyperactivity disorder (ADHD) is scarce. We collected diffusion-weighted magnetic resonance imaging data in 40 adults with ADHD and 36 neurotypical controls and used constrained spherical deconvolution-based tractography to reconstruct whole-brain structural connectivity networks. We used network-based statistic (NBS) and graph theoretical analysis to investigate differences in these networks between the ADHD and control groups, as well as associations between structural connectivity and ADHD symptoms assessed with the Adult ADHD Self-Report Scale or performance in the Conners Continuous Performance Test 2 (CPT-2). NBS revealed decreased connectivity in the ADHD group compared to the neurotypical controls in widespread unilateral networks, which included subcortical and corticocortical structures and encompassed dorsal and ventral attention networks and visual and somatomotor systems. Furthermore, hypoconnectivity in a predominantly left-frontal network was associated with higher amount of commission errors in CPT-2. Graph theoretical analysis did not reveal topological differences between the groups or associations between topological properties and ADHD symptoms or task performance. Our results suggest that abnormal structural wiring of the brain in adult ADHD is manifested as widespread intrahemispheric hypoconnectivity in networks previously associated with ADHD in functional neuroimaging studies.

Conceptual understanding and analysis conceptual difficulties of nervous system: from the perspective of pre-service biology teachers

<p><span lang="EN-GB">The aims of this study were to describe level of conceptual understanding and conceptual difficulties on the nervous system from the perspective of pre-service biology teachers. This research was used cross-sectional research design with a sample of 30 participants was selected randomly. The six essays that were created using the conceptual understanding indicator, and twelve multiple-choice questions that were created in the form of three-tier examinations, and the open questionnaires developed to detect challenging concepts in the nervous system make up the instrument. Data has been analysed using descriptive statistics. Data analysis indicates that the average score of conceptual understanding of pre-service biology teachers is 67.33 belongs to the medium category. For the highest percentage of conceptual understanding indicator is ability to exemplify concepts (86.67%) and the other hand, lower percentage is indicators of ability to connect various concepts (46.67%). The best concept for conceptual understanding especially about sub-matter of structure and function of neurons, central and autonomy nerves. Conversely, for the nervous system concepts that are recognized as challenging, particularly the concepts of physiology, impulse transmission mechanism, and somatic nerve inertia. The lack of conceptual understanding caused by complexity of subject, abundance of terminology, and intangible things. The study is expected to be used mainly for development of various learning methods or media that viewed as suitable for improving conceptual understanding of and reducing conceptual difficulties.</span></p>

Neuropeptides of root-knot nematodes: functional significance in parasite locomotions (short review)

The purpose of the research is to analyze the literature devoted to the study of the physiological role and functional significance of biologically active substances: FMRFamide-like neuropeptides in the locomotion of root-knot nematodes Meloidogyne incognita, M. minor, M. hapla and M. graminicola using immunological, phylogenetic, molecular and bioinformatic research methods.Results and discussion. The present work shows the importance of endogenous FMRFamide-like neuropeptides (FLPs) in such behavioral reactions of plant nematodes as locomotion, which ensures the vital activity of plant parasites; the functional significance of flp genes in the neurobiology of root-knot nematodes is discussed. It was especially noted that the main physiological and functional characteristics of endogenous FLP in root-knot nematodes were obtained as a result of studies of the functional role of the flp genes encoding these neuropeptides. In the nematodes M. incognita and M. graminicola, components of the peptidergic nervous system were identified in the nervous structures: FMRFamide-like positive immunoreactivity, FLP, flp genes encoding neuropeptides, and G-protein-coupled receptors (GPCR) activated by these neuropeptides. It was shown that the main functional characteristics of endogenous FLPs in nematodes were obtained using one of the methods of reverse genetics, i.e., flp genes knockdown in shadow by means of RNA-interference. It has been established that FLP cause two types of physiological effects on the somatic muscles of root-knot nematodes – stimulation of the locomotor activity of the muscles and its inhibition. In most works, the data obtained on the physiological effects of neuropeptides on the locomotor activity of phytonematodes are considered with a view to their possible use in the development of new targeted anthelmintic drugs.

Brief isoflurane administration as an adjunct treatment to control organophosphate-induced convulsions and neuropathology.

Organophosphate-based chemical agents (OP), including nerve agents and certain pesticides such as paraoxon, are potent acetylcholinesterase inhibitors that cause severe convulsions and seizures, leading to permanent central nervous system (CNS) damage if not treated promptly. The current treatment regimen for OP poisoning is intramuscular injection of atropine sulfate with an oxime such as pralidoxime (2-PAM) to mitigate cholinergic over-activation of the somatic musculature and autonomic nervous system. This treatment does not provide protection against CNS cholinergic overactivation and therefore convulsions require additional medication. Benzodiazepines are the currently accepted treatment for OP-induced convulsions, but the convulsions become refractory to these GABAA agonists and repeated dosing has diminishing effectiveness. As such, adjunct anticonvulsant treatments are needed to provide improved protection against recurrent and prolonged convulsions and the associated excitotoxic CNS damage that results from them. Previously we have shown that brief, 4-min administration of 3%-5% isoflurane in 100% oxygen has profound anticonvulsant and CNS protective effects when administered 30min after a lethal dose of paraoxon. In this report we provide an extended time course of the effectiveness of 5% isoflurane delivered for 5min, ranging from 60 to 180min after a lethal dose of paraoxon in rats. We observed substantial effectiveness in preventing neuronal loss as shown by Fluoro-Jade B staining when isoflurane was administered 1h after paraoxon, with diminishing effectiveness at 90, 120 and 180min. In vivo magnetic resonance imaging (MRI) derived T2 and mean diffusivity (MD) values showed that 5-min isoflurane administration at a concentration of 5% prevents brain edema and tissue damage when administered 1h after a lethal dose of paraoxon. We also observed reduced astrogliosis as shown by GFAP immunohistochemistry. Studies with continuous EEG monitoring are ongoing to demonstrate effectiveness in animal models of soman poisoning.

Selective chronic recording in small nerve fascicles of sciatic nerve with carbon nanotube yarns in rats

Objective. The primary challenge faced in the field of neural rehabilitation engineering is the limited advancement in nerve interface technology, which currently fails to match the mechanical properties of small-diameter nerve fascicles. Novel developments are necessary to enable long-term, chronic recording from a multitude of small fascicles, allowing for the recovery of motor intent and sensory signals. Approach. In this study, we analyze the chronic recording capabilities of carbon nanotube yarn electrodes in the peripheral somatic nervous system. The electrodes were surgically implanted in the sciatic nerve’s three individual fascicles in rats, enabling the recording of neural activity during gait. Signal-to-noise ratio (SNR) and information theory were employed to analyze the data, demonstrating the superior recording capabilities of the electrodes. Flat interface nerve electrode and thin-film longitudinal intrafascicular electrode electrodes were used as a references to assess the results from SNR and information theory analysis. Main results. The electrodes exhibited the ability to record chronic signals with SNRs reaching as high as 15 dB, providing 12 bits of information for the sciatic nerve, a significant improvement over previous methods. Furthermore, the study revealed that the SNR and information content of the neural signals remained consistent over a period of 12 weeks across three different fascicles, indicating the stability of the interface. The signals recorded from these electrodes were also analyzed for selectivity using information theory metrics, which showed an information sharing of approximately 1.4 bits across the fascicles. Significance. The ability to safely and reliably record from multiple fascicles of different nerves simultaneously over extended periods of time holds substantial implications for the field of neural and rehabilitation engineering. This advancement addresses the limitation of current nerve interface technologies and opens up new possibilities for enhancing neural rehabilitation and control.

Dysfunction of peripheral somatic and autonomic nervous system in patients with severe forms of Crohn's disease on biological therapy with TNFα inhibitors-A single center study.

Crohn's disease (CD) can be associated with a wide range of extraintestinal manifestations (EIMs), including neurological ones. Published studies differ in their conclusions about the epidemiology and etiopathogenesis of neurological EIMs. The aims of this study were to demonstrate the presence and find risk factors of peripheral (somatic and autonomic) neuropathy patients with severe CD on anti-TNFα biological therapy. A clinical examination focusing on detection of peripheral sensor-motor nervous dysfunction (including Sudoscan) and examination of autonomic nervous system dysfunction (using Ewing´s battery tests and spectral analysis) together with laboratory tests and collection of demographic data followed by administration of questionnaires were performed on a total of 30 neurologically asymptomatic outpatients with severe CD on anti-TNFα biological therapy. Peripheral sensor-motor nervous function via clinical neurological examination was pathological in 36.7% and Sudoscan in 33.3% of cases. Statistically significant associations between vibration perception test and age, CD and biological therapy duration, body mass index and Crohn's Disease Activity Index were proved while statistically significant associations between temperature perception test and age and BMI were proved as well. Additionally, a decrease of total protein in a patient´s serum below the physiological cut-off in the 6 months prior to measurement was associated with a pathological result of a Sudoscan. Cardiovascular autonomic neuropathy based on Ewing´s battery tests was present in 56.7% of patients, no statistically significant risk factors were found. Our peripheral neuropathy questionnaire correlated with the results of the Sudoscan test and some tests of the clinical examination of peripheral sensor-motor nervous function (discriminatory contact perception test, temperature perception test). This study demonstrated a relatively high prevalence of peripheral (especially autonomic) neuropathy and verified some risk factors for the development of peripheral somatic neuropathy in asymptomatic patients with severe form of CD on anti-TNFα biological therapy.

Intraoperative Monitoring of the External Urethral Sphincter Reflex: A Novel Adjunct to Bulbocavernosus Reflex Neuromonitoring for Protecting the Sacral Neural Pathways Responsible for Urination, Defecation and Sexual Function.

Intraoperative bulbocavernosus reflex neuromonitoring has been utilized to protect bowel, bladder, and sexual function, providing a continuous functional assessment of the somatic sacral nervous system during surgeries where it is at risk. Bulbocavernosus reflex data may also provide additional functional insight, including an evaluation for spinal shock, distinguishing upper versus lower motor neuron injury (conus vs. cauda syndromes) and prognosis for postoperative bowel and bladder function. Continuous intraoperative bulbocavernosus reflex monitoring has been utilized to provide the surgeon with an ongoing functional assessment of the anatomical elements involved in the S2-S4 mediated reflex arc including the conus, cauda equina and pudendal nerves. Intraoperative bulbocavernosus reflex monitoring typically includes the electrical activation of the dorsal nerves of the genitals to initiate the afferent component of the reflex, followed by recording the resulting muscle response using needle electromyography recordings from the external anal sphincter. Herein we describe a complementary and novel technique that includes recording electromyography responses from the external urethral sphincter to monitor the external urethral sphincter reflex. Specialized foley catheters embedded with recording electrodes have recently become commercially available that provide the ability to perform intraoperative external urethral sphincter muscle recordings. We describe technical details and the potential utility of incorporating external urethral sphincter reflex recordings into existing sacral neuromonitoring paradigms to provide redundant yet complementary data streams. We present two illustrative neurosurgical oncology cases to demonstrate the utility of the external urethral sphincter reflex technique in the setting of the necessary surgical sacrifice of sacral nerve roots.

Allostasis and Homeostasis: Dynamic Adaptive Systems from a Neurophysiological Perspective

Homeostasis is a well-known physiological principle that refers to a stable internal environment. Allostasis, on the other hand, is a more recently understood physiological principle based on predictive control, in which the setpoint of the internal environment is dynamically adjusted to increase control efficiency. The concept of allostasis continues to evolve with advances in the field of neuroscience. This overview introduces some new findings and broadens the concept of allostasis to apply it to the inter-regulation of the cognitive, somatic, and autonomic nervous systems. This article will describe on way in which biological systems alter themselves to adapt to their external and internal environments.

Effects of autonomic nervous system disorders on male infertility.

The male reproductive functions are largely regulated by the autonomic nervous system. Male sexual behavior and fertility primarily depend on the normal function of the higher neural centers related to the autonomic nervous system, the hypothalamic-pituitary-gonadal axis, the autonomic nervous components within the spinal cord and spinal nerves, and certain somatic nerves in the pelvic floor. In this review article, we will summarize the role of the autonomic nervous system in regulating male reproductive capabilities and fertility, its impact on male infertility under abnormal conditions, including the role of drug-induced autonomic nervous dysfunctions on male infertility. The main purpose of this article was to provide an overview of the effects of autonomic nervous dysfunction on male reproductive function and shed light on the potential therapeutic target for male infertility.

Morphology and Chemical Messenger Regulation of Echinoderm Muscles.

The muscular systems of echinoderms play important roles in various physiological and behavioral processes, including feeding, reproduction, movement, respiration, and excretion. Like vertebrates, echinoderm muscle systems can be subdivided into two major divisions, somatic and visceral musculature. The former usually has a myoepithelial organization, while the latter contains muscle bundles formed by the aggregation of myocytes. Neurons and their processes are also detected between these myoepithelial cells and myocytes, which are capable of releasing a variety of neurotransmitters and neuropeptides to regulate muscle activity. Although many studies have reported the pharmacological effects of these chemical messengers on various muscles of echinoderms, there has been limited research on their receptors and their signaling pathways. The muscle physiology of echinoderms is similar to that of chordates, both of which have the deuterostome mode of development. Studies of muscle regulation in echinoderms can provide new insights into the evolution of myoregulatory systems in deuterostomes.

Somatic and autonomic nerve density of the urethra, periurethral tissue, and anterior vaginal wall: an immunohistochemical study in adult female cadavers.

Retropubic procedures may disrupt nerves supplying the pelvic viscera; however, knowledge of pelvic neuroanatomy is limited. We sought to characterize somatic and autonomic nerve density within the urethra, periurethral tissue, and anterior vagina. Axial sections were obtained from pelvic tissue harvested from female cadavers ≤24 h from death at three anatomical levels: the midurethra, proximal urethra, and upper trigone. Periurethral/perivesical tissue was divided into medial and lateral sections, and the anterior vagina into middle, medial, and lateral sections. Double immunofluorescent staining for beta III tubulin (βIIIT), a global axonal marker, and myelin basic protein (MBP), a myelinated nerve marker, was performed. Threshold-based automatic image segmentation distinguished stained areas. Autonomic and somatic density were calculated as percentage of tissue stained with βIIIT alone, and with βIIIT and MBP respectively. Statistical comparisons were made using nonparametric Friedman tests. Six cadavers, aged 22-73, were examined. Overall, autonomic nerve density was highest at the midurethral level in the lateral and middle anterior vagina. Somatic density was highest in the external urethral sphincter (midurethra mean 0.15%, SD ±0.11; proximal urethra 0.19%, SD ±0.19). Comparison of annotated sections revealed significant differences in autonomic density among the lateral, medial, and middle vagina at the midurethra level(0.71%, SD ±0.48 vs 0.60%, SD ±0.48 vs 0.70%, SD ±0.63, p=0.03). Autonomic density was greater than somatic density in all sections. Autonomic and somatic nerves are diffusely distributed throughout the periurethral tissue and anterior vagina, with few significant differences in nerve density among sections analyzed. Minimizing tissue disruption near urethral skeletal muscle critical for urinary continence may prevent adverse postoperative urinary symptoms.

Participation of Modulating Rhythms of the Decasecond Range in Intersystem Correlation Interactions in Early Rat Ontogeny.

On newborn non-narcotized 1-day-old (P1) and 16-day-old (P16) rats, a detailed analysis of intersystem somatovisceral interactions (ISI) mediated by decasecond (D1, 5-50 sec) range modulating rhythms was performed. Correlation interactions of the main body systems - cardiovascular, respiratory and somatomotor systems in norm and under conditions of changes in the level of cholinergic regulation were studied. Spectral correlation analysis was used to determine the participation of D1 range modulating rhythms in ISI. It was found that at P1, D1 range rhythms do not play a significant role in integrative processes. In P16 the activation of cholinergic structures, caused by the introduction of the acetylcholinesterase inhibitor physostigmine (eserine) leads to significant disturbances in the degree of correlation in the D1 range. Blockade of muscarinic and nicotinic cholinoreceptors does not alter the degree of correlation of systemic pairs in the slow-wave region (D1-low, 8-50 sec) of the D1 range. Under the influence on the cholinoreactive structures, the most significant changes in the degree of correlation in the ISI affect the somatorespiratory systemic pair. The results obtained indicate that the representation of the slow-wave components of D1 range modulating rhythms involved in the ISI increase during the first weeks of postnatal ontogeny. Changes in the level of cholinergic activation do not directly influence on ISI mediated by D1-low sub-band rhythms.

Соматический пул белка, мышечная масса и саркопения как критерии нутритивного статуса в педиатрии

Введение. Нутритивный статус отражает взаимосвязь потребления и метаболизма пищевых веществ в виде объективных параметров тела, его биологических сред и компонентов. Результаты. Представлен обзор литературы. Для оценки нутритивного статуса используются доступные антропометрические параметры, имеющие доказанное клиническое и прогностическое значение (масса тела, индекс массы тела и др.). Тем не менее эти показатели не отражают в полной мере компонентный состав тела, что критически важно при некоторых заболеваниях и состояниях. Более информативным показателем является соматический пул белка – запас/физиологический резерв аминокислот в организме, который обеспечивает пул скелетно-мышечной массы. Мышечная ткань является основным резервом аминокислот в организме, она становится донором необходимого белка для репарации и обеспечения повышенных нужд в острую фазу заболевания/лечения пациентов с гиперкатаболизмом при хронических заболеваниях, травмах, в онкопедиатрии и др. Важным критерием оценки нутритивного статуса является анализ риска саркопении. Саркопения (снижение мышечной массы) и динапения (снижение мышечной силы) приводят к увеличению риска заболеваний, смертности и ухудшению качества жизни. Саркопения может иметь место не только при гиперкатаболизме, но и у детей с ожирением, что увеличивает риски инсулинорезистентности и метаболического синдрома, при этом кратно потенциируется неблагоприятный прогноз. Это свидетельствует о необходимости применения различных методов оценки мышечной массы/силы, а также выявления риска развития саркопении для адекватной, дифференцированной в зависимости от этиологических факторов нутритивной поддержки и других факторов реабилитации. В коррекции саркопении важно учитывать ее этиопатогенез. Заключение. Увеличение белка в суточном рационе является эффективным средством, чтобы замедлить или предотвратить мышечный катаболизм и саркопению, в том числе у детей с ожирением. Однако восстановление скелетно-мышечной массы и профилактика саркопении это не изолированное увеличение белка в рационе, а полноценное питание с достаточным содержанием легкоусвояемого белка и с поиском и возможным устранением причин саркопении и факторов, способствующих ее усугублению. Background. Nutritional status reflects the relationship between consumption and metabolism of nutrients, in the form of objective parameters of the body, its biological media and components. Results. A review of the literature is presented. To assess nutritional status, available anthropometric parameters that have proven clinical and prognostic significance (body weight, body mass index, etc.) are used. However, these indicators do not fully reflect the component composition of the body, which is critically important for some diseases and conditions. A more informative indicator is the somatic protein pool – the reserve/physiological reserve of amino acids in the body, which provides a pool of skeletal muscle mass. Muscle tissue is the main reserve of amino acids in the body; it becomes a donor of the necessary protein for repair and to meet increased needs in the acute phase of the disease/treatment of patients with hypercatabolism in chronic diseases, injuries, oncopediatrics, etc. An important criterion for assessing nutritional status is the analysis of the risk of sarcopenia. Sarcopenia (decreased muscle mass) and dynapenia (decreased muscle strength) lead to increased risk of disease, mortality, and poor quality of life. Sarcopenia can occur not only in hypercatabolism, but also in obese children, which increases the risks of insulin resistance and metabolic syndrome, while the unfavorable prognosis is greatly enhanced. This indicates the need to use various methods for assessing muscle mass/strength, as well as identifying the risk of developing sarcopenia for adequate differentiated nutritional support and other rehabilitation factors depending on the etiological factors. In the correction of sarcopenia, it is important to take into account its etiopathogenesis. Increasing protein in the daily diet is effective in slowing or preventing muscle catabolism and sarcopenia, including in obese children. However, restoration of skeletal muscle mass and prevention of sarcopenia is not an isolated increase in protein in the diet, but a nutritious diet with a sufficient content of easily digestible protein and the search and possible elimination of the causes of sarcopenia and factors contributing to its aggravation.