Continued fertilizations have led to soil phosphorus (P) concentrations that exceed those required for optimal plant growth. In this study, P accumulation and leaching were investigated in 1-m profile of three reconstructed soils (sandy, SDY; clay, CLY; peaty, PTY) typical of the Po Valley (northern Italy) following long-term (44 years) farmyard manure and mineral applications and untreated. Soil test P was determined by extracting with ammonium oxalate (POx), Mehlich-3 (PM3) and Olsen (POls) solutions, whereas soil P release was estimated by water extraction (PH2O). Two single-point sorption indices (PSI19.4 and PSI50) were used to assess the P sorption capacity in these soils. The relationship between extractable P forms and water P was also modelled as a segmented line.Soil test P resulted as being affected by type of soil, treatment and depth. Farmyard manure increased the P forms by 7.7-fold that of untreated down to 50cm in CLY and PTY, and 28-fold untreated down to 90cm in SDY, thus indicating both accumulation and leaching of P. The P sorption indices were affected by type of soil, treatment and depth. P accumulation was also confirmed by increments of PSI50 in the manure-treated deeper layers, likely due to increased organic matter and/or precipitation and co-precipitation of P with carbonates in these soils. Although mineral fertilization also increased extractable P it was with lower magnitude than manure on P leaching and P sorption indices. From the segmented lines between PH2O and extractable P, the presence of change points in CLY and PTY revealed PH2O concentrations above the environmental threshold values. In the light-textured SDY soils, the low P sorption capacity and absence of change points evidenced high P leaching and potential contamination of surface and ground waters, in particular when manure was used.