Toluene Solution Research Articles

Direct Extraction of Sodium Hydroxide by Calix[4]pyrrole-Based Ion-Pair Receptors.

Described in this work are calix[4]pyrrole-based ion-pair receptors, cis/trans-1 and cis/trans-2, designed for the extraction of sodium hydroxide. An X-ray diffraction analysis of a single crystal of the cis-1·NaOH isomer isolated from a mixture of cis/trans-1 revealed a unique dimeric supramolecular structure. An average dimer in toluene-d8 solution was inferred on the basis of diffusion-ordered spectroscopy (DOSY). Support for the proposed stoichiometry came from density functional theory (DFT) calculations. The structural stability of the dimeric cis-1·NaOH complex in toluene solution was further confirmed by ab initio molecular dynamics (AIMD) simulation with explicit representation of solvent. Under conditions of liquid-liquid extraction (LLE), purified receptors cis- and trans-2 were both found to remove NaOH from a pH 11.01 aqueous source phase into toluene with extraction efficiencies (E%) of 50-60% when used equimolar to NaOH. However, in all cases, precipitation was observed. Complexities associated with precipitation could be avoided by immobilization of the receptors onto a chemically inert poly(styrene) resin by means of solvent impregnation. The use of solvent-impregnated resins (SIRs) eliminated precipitation in solution while retaining the extraction efficiency toward NaOH. This allowed both the pH and salinity of the alkaline source phase to be lowered.

In-situ calibration method for thermocouples in accelerating rate calorimeter based on multiple fixed-points and Joule heat

To solve the problem of temperature drift of thermocouples in accelerating rate calorimeter caused by long-term operation, an in-situ calibration method based on multiple fixed-points and Joule heat is proposed in this article. Firstly, the heat transfer model of the calorimeter is established, and the validity of the method is verified by numerical simulation. Secondly, a multiple fixed-points graphite calibrator and a cylindrical electronic resistance element are designed. Finally, the in-situ calibration is carried out. The calibration results show that the maximum permissible measurement error of the sample thermocouple after calibration is better than 0.220 °C and that the bias of consistency in-situ calibration method is smaller than 0.110 °C. In addition, a di‑tert‑butyl peroxide in toluene solution with a mass percent of 20% is selected as the experimental sample. The sample experiment results show that the kinetic and thermodynamic parameters are closer to the reference values after thermocouples calibration.

Cyclo-penta-dienone triisocyanide iron complexes: general synthesis and crystal structures of tris-(2,6-di-methyl-phenyl isocyanide)(η4-tetra-phenyl-cyclo-penta-dienone)iron and tris-(naphthalen-2-yl iso-cyanide)(η4-tetra-phenyl-cyclo-penta-dienone)iron acetone hemisolvate.

Irradiation of a toluene solution containing cyclo-penta-dienone tricarbonyl iron complexes and isocyanides with blue LEDs afforded the formation and isolation of 12 triisocyanide complexes, two of which, namely tris-(2,6-di-methyl-phenyl isocyanide)(η4-tetra-phenyl-cyclo-penatedienone)iron, [Fe(C9H9N)3(C29H20O)], and tris-(naphthalen-2-yl isocyanide)(η4-tetra-phenyl-cyclo-penatedienone)iron acetone hemisolvate, [Fe(C11H7N)3(C29H20O)]2·C3H6O, could be characterized crystallographically. The air-stable compounds were purified by column chroma-tography and were characterized by 1H NMR, 13C NMR, elemental analysis and HRMS. NMR and XRD data indicate generally more electron-rich Fe0 centers compared to the corresponding tricarbonyl compounds.

Förster-free Effect Fluorescence Quenching of 5,10,15,20-tetraphenylporphyrin in Solution.

The absorption of exciting light by an inner filter may be the cause of large errors in estimating the efficiency of fluorescence quenching. We performed fluorescence measurements of 5,10,15,20-tetraphenylporphyrin toluene solution in a wide concentration range. We have shown a Förster-free fluorescence quenching in a right-angle geometry experiment, caused by second order inner filter. We proposed to perform measurements in a front-surface geometry to investigate the nature of quenching not related to the inner filters. We have shown that concentration effects in tetraphenylporphyrin solutions in toluene are not accompanied by a decrease in the fluorescence intensity in a front-surface geometry at concentrations of 10- 3-10- 7mol/l. It was possible to separate the phenomena of the internal filters from the processes occurring in a liquid medium. Our results are of great importance due to the widespread fundamental research of porphyrin-based dyes properties.

Runaway reaction investigation of di‑tert‑butyl peroxide using adiabatic calorimetry

Thermal safety assessment is an effective means to put an end to frequent chemical accidents. The conventional safety assessment generally uses differential scanning calorimetry (DSC) or accelerating rate calorimeter (ARC), while in this work the adiabatic calorimetry technique is applied to study the thermal runaway reaction for the first time. Adiabatic calorimeter (AC) has an excellent adiabatic and vacuum environment, and the process simulation of adiabatic runaway is more real in theory. The thermal decomposition reaction of 20% di‑tert‑butyl peroxide (DTBP) /toluene solution was measured, and the kinetic parameters and model of DTBP decomposition in the adiabatic reaction process were obtained by fitting the temperature rise rate-temperature data in the thermal runaway process. These research results not only show that the adiabatic calorimetry is competent for the determination and thermal risk assessment of adiabatic runaway reactions, but also provide an important reference for broadening the application of adiabatic calorimetry.

Extraction of quinoline from toluene solution of wash oil using 1‑butyl‑3-methylimidazolium hexafluorophosphate ([BMIM] [PF6]): Experimental and molecular dynamics simulations

In this paper, a green solvent [BMIM] [PF6] ionic liquid was selected for the extraction and separation of quinoline from simulated wash oil. The effects of extraction temperature, extraction time, and solvent oil ratio on the extraction of quinoline from wash oil were studied. The optimal process parameters were obtained by changing single factor conditions and optimizing the experiment using response surface methodology. When the temperature is 71 ℃, the time is 60 min, and the solvent oil ratio is 3:5, the extraction efficiency can reach 67%. The ionic liquids were recovered and their properties were investigated by infrared spectroscopy. The mechanism of quinoline extraction by ionic liquids was analyzed using infrared spectroscopy and GROMACS kinetic simulation calculations.

Vanadium Complexes Derived from O,N,O-tridentate 6-bis(o-hydroxyalkyl/aryl)pyridines: Structural Studies and Use in the Ring-Opening Polymerization of ε-Caprolactone and Ethylene Polymerization

Interaction of [VO(OiPr)3] with 6-bis(o-hydroxyaryl)pyridine, 2,6-{HOC(Ph)2CH2}2(NC5H3), LH2, afforded [VO(OiPr)L] (1) in good yield. The reaction of LNa2, generated in-situ from LH2 and NaH, with [VCl3(THF)3] led to the isolation of [VL2] (2) in which the pyridyl nitrogen atoms are cis; a regioisomer 3∙2THF, in which the pyridyl nitrogen atoms are trans, was isolated when using [VCl2(TMEDA)2]. The reaction of the 2,6-bis(o-hydroxyalkyl)pyridine {HOC(iPr)2CH2}2(NC5H3), L1H2, with [VO(OR)3] (R = nPr, iPr) led, following work-up, to [VO(OR)L1] (R = nPr (4), iPr (5)). Use of the bis(methylpyridine)-substituted alcohol (tBu)C(OH)[CH2(C5H3Me-5)]2, L2H, with [VO(OR)3] (R = Et, iPr) led to the isolation of [VO(μ-O)(L2)]2 (6). Complexes 1 to 6 have been screened for their ability to act as pre-catalysts for the ring opening polymerization (ROP) of ε-caprolactone (ε-CL), δ-valerolactone (δ-VL), and rac-lactide (r-LA) and compared against the known catalyst [Ti(OiPr)2L] (I). Complexes 1, 4–6 were also screened as catalysts for the polymerization of ethylene (in the presence of dimethylaluminium chloride/ethyltrichloroacetate). For the ROP of ε-CL, in toluene solution, conversions were low to moderate, affording low molecular weight products, whilst as melts, the systems were more active and afforded higher molecular weight polymers. For δ-VL, the systems run as melts afforded good conversions, but in the case of r-LA, all systems as melts exhibited low conversions (<10%) except for 6 (<54%) and I (<39%). In the case of ethylene polymerization, the highest activity (8600 Kg·mol·V−1bar−1h−1) was exhibited by 1 in dichloromethane, affording high molecular weight, linear polyethylene at 70 °C. In the case of 4 and 5, which contain the propyl-bearing chelates, the activities were somewhat lower (≤1500 Kg·mol·V−1bar−1h−1), whilst 6 was found to be inactive.

Novel silver-morphine-functionalized polypropylene (AgPP-mrp) nanocomposite for the degradation of dye removal by multivariate optimization approach.

As a novel adsorbent, an opioid silver-morphine-functionalized polypropylene was synthesized through a one-pot reaction at room temperature and successfully used for the simple one-pot photocatalytic degradation catalyst of methyl orange removal from wastewater. UV spectral analysis reveals a special reference to the excitation of surface plasmon resonance as the main characteristic of the polymer-Ag nanocomposite in toluene solution peak at 420nm in AgPP-mrp catalyst.The 1H NMR spectrum showed no sign of Ag NP peaks revealing small size distribution in the channels of morphine-functionalized polypropylene polymer. The morphology of silver nanoparticle-doped polymer through scanning electron microscopy (SEM-EDX) reveals PP-mrp with continuous matrix and Ag NPs (0.87 wt%). Furthermore, photocatalytic degradation of methyl orange was investigated on AgPP-mrp catalyst spectrophotometrically under solar irradiation in waste effluent, demonstrating high degradation efficiency. According to experimental findings, silver nanoparticles (AgPP-mrp) achieved high degradation capacities of 139mg/g equivalent to 97.4% of photodegradation in a little period of time (35min), as associated with previously stated materials and follow pseudo-second-order kinetic degradation tail of a high regression coefficient (R2 = 0.992). The suggested techniques offer a linear reaction for MO over the pH range of 1.5 to 5 and a degradation temperature of 25 to 60°C. Central composite design and response surface methodology statistics recommend pH of the reaction medium and time as important variables for methyl orange degradation on AgPP-mrp photocatalytic. AgPP-mrp on the photocatalytic phenomenon based on heterojunction catalytic design producing electron holes (e-), as well as superoxides for the successful degradation of methyl orange.

The properties of hardness and swelling of styrene butadiene rubber/silica/oleamide composites

This paper reports the results of the effects of oleamide addition on hardness, as one of the mechanical behaviours, of styrene butadiene rubber/silica (SBR/silica) composite. The base SBR was compounded with silica, as a reinforcing filler, and others compounding chemicals. The silica was compounded into the rubber matrix at 30 phr and others compounding chemicals were added based on the semi-efficient rubber compounding formulation. The compounding has been done using opened two-roll mills. Oleamide was added to the SBR/silica to improve the rubber-to-filler interaction and the mechanical properties of the SBR/silica composite. The oleamide was a lab-synthesizing chemical and was added into the SBR/silica with 4 different concentrations (2, 4, 6 and 8 phr). The SBR/silica compounds with and without oleamide were vulcanized at 150 °C using a typical cure meter. From the results; it was noticed that the hardness of the SBR/Si composite was increased as the oleamide was added to the SBR/Si compound. It was considered that the hardness improvement was attributed to the result of crosslinks improvement of the NR/silica/oleamide composites. The additions of the oleamide convert the rubber composites into more resistance to swelling. A greater degree of crosslinks would increase the swelling rejection/resistance but decrease the interpenetration of the toluene solution to the rubber composites. The oleamide acted as an additive which improved the properties of swelling and hardness of the SBR/Si mixtures/compounds and, a 2 phr of oleamide addition was the optimum concentration for the SBR/Si compound.

Reversible ligand exchange of triphenylphosphine with thiols on gold nanoparticles for Janus modification and catalyst recycling

Separation and regeneration of ultrafine catalysts in reaction system still remain challenging. In this study, we demonstrate a simple route to recycle gold nanoparticle (Au NP) catalysts by alternating surface wettability. Hydrophilic Au NPs protected with 3-mercapto-1,2-propanediol (MPD) were applied to catalyze the reduction of 4-nitrophenol (4-NP) using sodium borohydride as reductant, and were then separated from the suspension by interfacial ligand exchange with a toluene solution containing triphenylphosphine (TPP), led to amphiphilic Janus Au NPs with both MPD and TPP ligands at the toluene-water interface. The catalyst was regenerated by a reversible ligand exchange with a MPD aqueous solution. Experiments show that interfacial ligand exchange between phosphines and thiols on gold surface is reversible, although the forward and backward rates are different. As a result, the Au NP catalyst capped with MPD exhibited an excellent catalytic activity with an apparent rate constant (k) of 2.79 min−1, and the performance could be maintained for more than 10 regeneration cycles. The facile conversion between hydrophilic and amphiphilic Janus Au NPs provides a new pathway to explore monophasic and multiphasic reactions with convenient catalyst separation and regeneration.

β,β-Directly Linked Porphyrin Rings: Synthesis, Photophysical Properties, and Fullerene Binding.

Cyclic porphyrin oligomers have been studied as models for photosynthetic light-harvesting antenna complexes and as potential receptors for supramolecular chemistry. Here, we report the synthesis of unprecedented β,β-directly linked cyclic zinc porphyrin oligomers, the trimer (CP3) and tetramer (CP4), by Yamamoto coupling of a 2,3-dibromoporphyrin precursor. Their three-dimensional structures were confirmed by nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and single-crystal X-ray diffraction analyses. The minimum-energy geometries of CP3 and CP4 have propeller and saddle shapes, respectively, as calculated using density functional theory. Their different geometries result in distinct photophysical and electrochemical properties. The smaller dihedral angles between the porphyrin units in CP3, compared with CP4, result in stronger π-conjugation, splitting the ultraviolet-vis absorption bands and shifting them to longer wavelengths. Analysis of the crystallographic bond lengths indicates that the central benzene ring of the CP3 is partially aromatic [harmonic oscillator model of aromaticity (HOMA) 0.52], whereas the central cyclooctatetraene ring of the CP4 is non-aromatic (HOMA -0.02). The saddle-shaped structure of CP4 makes it a ditopic receptor for fullerenes, with affinity constants of (1.1 ± 0.4) × 105 M-1 for C70 and (2.2 ± 0.1) × 104 M-1 for C60, respectively, in toluene solution at 298 K. The formation of a 1:2 complex with C60 is confirmed by NMR titration and single-crystal X-ray diffraction.

Mono- and Bimetallic Aluminum Complexes Supported by Thioether-Amide Ligands: Synthesis, Characterization, and Application in the Ring Opening Polymerization of l-Lactide

Mono- and bimetallic aluminum complexes bearing bidentate NS or tetradentate NSSN ligands have been synthesized via reaction of AlMe3 with the corresponding proligand and spectroscopically characterized. In these complexes the aluminum centers are surrounded by anionic nitrogen and neutral sulfur atoms that, together with the carbon atoms of the alkyl groups, produce tetrahedral coordination geometries. In the bimetallic complexes the two metal centers are linked by a thioether-bridge of different lengths. The solid state structure of the complexes featuring the thioethyl or thiopropyl bridge were determined by X-ray diffraction studies. Both mono- and bimetallic aluminum complexes display fluxional behaviors in solution. After proper activation, all complexes were able to promote the ring opening polymerization of l-lactide in toluene solution. The polymerizations were well controlled as testified by the linear increase of the molecular weights with the conversions and the narrow polydispersity indexes. The highest activity was observed for the bimetallic complex bearing the NSSN ligand with the shortest bridge (ethyl bridge) with a turnover frequency of 11.6 molLA·molAl–1·h–1 (toluene, 80 °C). The activity decreased as the distance between the two metal centers increased. Moreover, the activity of the bimetallic complex with the ethyl bridge was higher than those of the monometallic ones. These findings suggest that a cooperative interaction between two aluminum centers is operative in the bimetallic complex with the shortest bridge.

Cineole – Decanoic acid hydrophobic natural Deep eutectic solvent for toluene absorption

The hydrophobic deep eutectic solvent formed by the equimolar combination of natural compounds cineole and decanoic acid is studied as an archetypical compound for aromatics (toluene) solubilization/extraction purposes. The physicochemical properties of toluene solutions in the solvents are studied as a function of composition and temperature. The nature of intermolecular forces between toluene and solvent molecules is analyzed using a minimal clusters approach through quantum chemistry methods. Classical molecular dynamics simulation on toluene solution allowed us to infer nanoscopic behavior and structuring of the toluene mixtures using static and dynamic approaches. Likewise, the possible biological effects of the considered solvent are studied considering its effect on lipid bilayers as a model of plasma membranes using coarse-grained molecular dynamics methods. The reported results provide a multiscale combined experimental and computational characterization of hydrophobic Deep Eutectics as suitable solvents for aromatic hydrocarbons.

Chiral perturbation on single benzene-based fluorophores: A structure/(chir)optical activity relationship study.

In this paper, we describe the synthesis and the (chir)optical properties of a novel series of circularly polarized luminescent emitters. These molecules involve a compact single benzene-based donor-acceptor fluorophore composed of two cyclic alkylamines as electron donors and a phthalonitrile moiety as electron acceptor linked to a configurationally stable BINOL acting as a chiral perturbation unit. These new compounds display fair quantum yields (up to 66%) with emission maxima around 500 nm in toluene solutions, and the study of their chiroptical properties has shown that the cyclic alkylamine's ring size affects significantly the luminescence dissymmetry factors, reaching 2.2×10-3 for the larger cyclic alkylamine moieties.

Ten-Membered Cyclodecatetraene Derivatives Including Two Gallium Atoms: Experimental and Theoretical Studies on Synthesis, Structures, and Their Transformations to Nine- and Five-Membered Gallacycles

The chemistry of medium-sized ring compounds, including group 13 elements, has scarcely been investigated. Herein, we report that 5,10-digallacyclodeca-1,3,6,8-tetraene derivatives Cl2Ga2C8R8 (R = Me (5a), Et (5b)) can be obtained by the reaction of GaCl3 and zirconacyclopentadiene Cp2ZrC4R4 (R = Me (2a), Et (2b), Cp: η5-C5H5) in toluene. X-ray crystal structure analysis revealed that compound 5b has a 10-membered cyclodecatetraene structure composed of two butadiene skeletons and two GaCl fragments. X-ray crystal structure analysis and IR measurement confirmed that compound 5b forms Ga2Cl2 four-membered rings via intermolecular Cl to Ga donor–acceptor interactions, which result in the formation of oligomeric chain structures in the solid state and toluene solution. The treatment of compound 5b with 2 equivalents of 4-(dimethylamino)pyridine (DMAP) proceeded via the coordination of DMAP to the Ga center and the ring contraction to give DMAP-coordinated five-membered gallole Cl(DMAP)GaC4Et4 (4bDMAP). The reaction of compound 5a with 2 equivalents of MesLi (Mes: 2,4,6-Me3C6H2) in toluene to form a 9,10-digallabicyclo[4.3.1]decatriene derivative (MesGaC8Me8)GaMes (6a) caused ring contraction. Theoretical investigation revealed that the 10-membered ring derivative, Cl2Ga2C8H8 (GaH-10), is formed by the dimerization of two gallole molecules, ClGaC4H4 (GaH-5), via the addition of two Ga–C bonds without an energy barrier. The immediate formation of GaH-10 was attributed to the transannular electronic interaction between the expanded empty p-orbital on the Ga atom and the electron-rich sp2 carbons of the C═C double bond in GaH-5. Moreover, GaH-10 and one of the isomer, nine-membered ring derivative (ClGaC8H8)GaCl, were found to be thermodynamically more stable, as compared to GaH-5.

Amplified spontaneous emission from CdSe/CdS/CdZnS quantum dot films

The dynamic and spectral characteristics of close-packed quantum dots excited by a ∼30 ps long laser pulse in a film prepared by a drop casting method from toluene solution were investigated. The photoluminescence wavelength of single excitons and the wavelength of amplified spontaneous radiation associated with a stimulated decay of biexcitons were obtained. From the spectrogram of the time-resolved photoluminescence the information was obtained about the dynamics of these two types of radiation. It was shown that the radiation peak maximum of single excitons appears by 60 ps later than the maximum of amplified spontaneous radiation, then it slowly becomes narrower and diminishes. The amplified spontaneous radiation lasts for 200 ps.

Synthesis and Properties of Sulfur-Containing Organophosphorus Extractants Based on Red Phosphorus, Alkyl Bromides, and Elemental Sulfur.

In order to obtain sulfur-containing organophosphorus compounds that are promising as extractants of heavy metals, the interaction of elemental phosphorus and sulfur with alkyl bromides catalyzed using strong bases was studied. According to the task, the reaction of non-toxic and non-flammable red phosphorus with alkyl bromides under conditions of phase transfer catalysts (PTC), followed by the introduction of elemental sulfur into the reaction medium, were studied. It is shown that alkyl bromides interact with red phosphorus when heated (95-105 °C, 5-8 h) under conditions of phase transfer catalysts (PTC) in a two-phase system: a 60% aqueous solution of KOH-toluene-benzyltriethylammonium chloride (BTEAC) forming a mixture of organophosphorus compounds along with alkylphosphines (57-60%), are the main reaction products; alkylphosphine oxides are also formed (40-43%). The introduction of elemental sulfur (solution in toluene) at the final stage of the process into the reaction mass cooled to 40-60 °C leads to the expected alkylphosphine sulfides, which are the result of the interaction of alkylphosphines with sulfur. The formation of complex mixtures of products prevents the release of target alkylphosphine sulfides in individual form. However, the synthesized mixture of alkylphosphine sulfides and alkylphosphine oxides without separation into individual components is promising for studying its extraction properties in relation to heavy metals. Testing of the extraction properties of synthesized mixtures of alkylphosphine sulfides and alkylphosphine oxides in relation to heavy metals (Ni, Co, Zn, Pb) and noble metals (Ag) showed that the resulting mixtures of tertiary phosphine oxides and phosphine sulfides are highly effective extractants. The degree of extraction in relation to Ni, Co, Zn, and Pb varies from 99.90 to 99.99%, and for Ag from 99.56 to 99.59%.

Elastic Electrically Conductive Composites Based on Vapor-Grown Carbon Fibers for Use in Sensors.

Elastic electrically conductive composites with an ethylene octene copolymer matrix (EOC) and vapor-grown carbon fibers (VGCF) were prepared by ultrasonication in a toluene solution, and their morphology, mechanical and electrical properties were also evaluated. EOC/CF composites were estimated for their mechanical and viscoelastic properties. The morphology of the composites was analyzed using scanning electron microscopy (SEM), and stress-strain curves were generated to measure the stress and tensile modulus of the composites. The experimental results were compared with various theoretical models, including the Burgers model, which separates viscoelastic behavior into several components. A dynamic mechanical analysis was also used to measure the composites' storage modulus, loss modulus, and damping factor at different frequencies. The composites' complex viscosity and storage modulus were increased with higher wt.% of CF, which enhances the elastic response. Electrical resistivity measurements were conducted on the composites and it was found that the resistivity decreased as the sample was loaded and increased as it was unloaded. Overall, the study provides insights into the mechanical and viscoelastic properties of EOC/CF composites, which could be helpful in developing sensors such as pressure/strain sensors.

About the crystallization of cyclic and linear poly(L-lactide)s in alcohol-initiated and Sn(II)2-ethylhexanoate-catalyzed ROPs of L-lactide conducted in solution

1-Hydroxymethylnaphtalene (HMN) or 11-bromoundecanol (BUND) were used as initiators and Sn(II) 2-ethylhexanoate (SnOct2) as catalyst for ROPs of L-Lactide (LA) at 115 °C in bulk or in 4 M and 2 M solutions in toluene. The LA/In ratio, the LA/Cat ratio and the time were varied. The matrix-assisted laser desorption/ionization time-of-flight (MALDI TOF) mass spectra exclusively displayed peaks of linear chains, when the ROPs were conducted in bulk. But in contrast to reports in the literature, mixtures of linear and cyclic poly(L-lactide) (PLA), were obtained, when the ROPs were performed in solution. The intensity distribution of the mass peaks of cyclic PLAs displayed a “saw-tooth pattern” after annealing in contrast to the mass peak distribution of the liner chains. This new phenomenon indicated that cyclic PLAs and linear PLAs crystallized in separate crystals from the same reaction mixture. This conclusion was confirmed by fractionated crystallization from 2 M solution, which confirmed that the cyclic PLAs nucleate and crystallize faster than the linear chains.

Phytochemcial Investigation of Extracts of Hedyotis herbacea

The phytochemical study was carried out on the Hedyotis herbacea plant, which belongs to the Rubiacea family and is traditionally claimed to have wound healing activity. The plant was extracted using cold maceration techniques with rising polarity solvents such as petroleum ether, chloroform, ethyl acetate and ethanol. The secondary metabolite present in the extract was found through preliminary phytochemical screening. Exracts were further subjected for isolation. Betulinic acid was isolated from the chloroform exract. Isolated Betulinic acid was weighed at 0.50 mg, and its presence was verified using TLC in a solvent solution of toluene, ethyl acetate, and glacial acetic acid (8.5:1.5: 0.02 v/v/v). Betulinic acid was further characterised by IR, NMR and Mass spectroscopy. The ability of Hedyotis herbacea to repair wounds can be further investigated by using betulinic acid.