Antibiotic Solution Research Articles

Stability Studies of the Dilution Series of Different Antibiotic Stock Solutions in Culture Medium Incubated at 37 °C.

The long-term stability of antibiotics in culture media remains underexplored in scientific literature. This study evaluated the stability of eight distinct antibiotic stock solutions-amoxicillin, cefotaxime, neomycin, oxytetracycline, florfenicol, enrofloxacin, colistin, and potentiated sulfonamide-and their 10-fold dilution series in tryptone soy broth (TSB) at 37 °C, over 12 days. Samples were collected immediately after preparation and on days 1, 2, 5, 7, 9, and 12, with active substance concentrations measured using ultra-high-performance liquid chromatography (UHPLC) coupled with mass spectrometry. The results indicated that among the ultrapure water stock solutions, neomycin, florfenicol, and potentiated sulfonamide maintained stability (>95%). Within the culture medium, florfenicol showed consistent stability (100%) throughout the study, potentiated sulfonamide experienced minor degradation (>85%), and neomycin underwent significant degradation. Amoxicillin, oxytetracycline, and colistin displayed considerable degradation in both solution types but were more stable in ultrapure water solutions. The stability of cefotaxime and enrofloxacin in ultrapure water solutions and in the medium was very similar when compared; however, 3.6% of the former and 88.7% of the latter remained detectable by day 12. These findings are crucial for minimum inhibitory concentration (MIC) assessments, especially in minimum bactericidal concentration (MBC) studies, and in experiments concerning long-term evolution and co-selection. This study underscores the necessity of stability assessments in culture media to validate future experimental outcomes.

Oxygen-deficient AgIO3 for efficiently photodegrading organic contaminants under natural sunlight

Defect engineering is regarded as an effective strategy to boost the photo-activity of photocatalysts for organic contaminants removal. In this work, abundant surface oxygen vacancies (Ov) are created on AgIO3 microsheets (AgIO3-OV) by a facile and controllable hydrogen chemical reduction approach. The introduction of surface Ov on AgIO3 broadens the photo-absorption region from ultraviolet to visible light, accelerates the photoinduced charges separation and migration, and also activates the formation of superoxide radicals (•O2−). The AgIO3-OV possesses an outstanding degradation rate constant of 0.035 min−1, for photocatalytic degrading methyl orange (MO) under illumination of natural sunlight with a light intensity is 50 mW/cm2, which is 7 and 3.5 times that of the pristine AgIO3 and C–AgIO3 (AgIO3 is calcined in air without generating Ov). In addition, the AgIO3-OV also exhibit considerable photoactivity for degrading other diverse organic contaminants, including azo dye (rhodamine B (RhB)), antibiotics (sulflsoxazole (SOX), norfloxacin (NOR), chlortetracycline hydrochloride (CTC), tetracycline hydrochloride (TC) and ofloxacin (OFX)), and even the mixture of organic contaminants (MO-RhB and CTC-OFX). After natural sunlight illumination for 50 min, 41.4% of total organic carbon (TOC) for MO-RhB mixed solution can be decreased over AgIO3-OV. In a broad range of solution pH from 3 to 11 or diverse water bodies of MO solution, AgIO3-OV exhibits attractive activity for decomposing MO. The MO photo-degradation process and mechanism over AgIO3-OV under natural sunlight irradiation has been systemically investigated and proposed. The toxicities of MO and its degradation intermediates over AgIO3-OV are compared using Toxicity Estimation Software (T.E.S.T.). Moreover, the non-toxicity of both AgIO3-OV catalyst and treated antibiotic solution (CTC-OFX mixture) are confirmed by E. coli DH5a cultivation test, supporting the feasibility of AgIO3-OV catalyst to treat organic contaminants in real water under natural sunlight illumination.

Synthesis and characterization of La-doped SnO2 nanoparticles with different shape: A comprehensive study on morphology, structure, and photocatalytic efficiency for eco-friendly wastewater treatment

New controllable synthetic approach to obtain of La-doped SnO2 nanoparticles based on the combination of co-precipitation method with post synthetical hydrothermal treatment has been developed. Nanoparticles with various shape (3 nm spheres and 5 nm cubes) and structural parameters, i.e. different amount of oxygen vacancies and defects, and different dopant concentrations of 11 mol% and 33 mol% were fully characterized using XRD, FTIR, XPS and Raman spectroscopy, TEM, SSA evaluation, optical absorption spectra study, luminescence spectroscopy, including quantum chemical calculations.It was shown that hydrothermal treatment (240 °C during 6 h) promotes nanoparticle growth via the mechanism of oriented attachment. Original computational approach based on the calculation of interaction energies between ions and crystal facets was engaged to study the growth process.The presence of intrinsic photoluminescence (406, 414, 438, 460, 500 and 676 nm) for all obtained samples was established, determined by a complex set of different parameters for sub-10 nm nanoparticles.Photocatalytic efficiency of 95 % on the example of methylene blue under commercially available LED lamp described via quantum chemical modelling and tested on the example of a colorless antibiotic solution. A clear dependance of the morphological and structural parameters on the photocatalytic properties has been obtained and studied in detail.Novel concept based on a combination of chemical and computational method, for effective visible-light driven photocatalyst synthesis with potential antibacterial properties for complex eco-friendly wastewater treatment was proposed.

Development of ultrathin 2D layered titanate Pt/K2Ti4O9/rGO nanocomposites for enhanced visible active photocatalytic detoxification of harmful organic pollutants

In this work, different wt% of rGO-loaded Pt-K2Ti4O9 (x = 2.5, 5, 7.5, 10, and 12.5 wt%) nanocomposites were prepared by a facile hydrothermal process and used for the degradation of two different dyes such as congo red (CR) methylene blue (MB) and tetracycline (TC) antibiotic solution under visible light illumination. The bare K2Ti4O9 (labeled as KTO) and the most active Pt-K2Ti4O9/5% rGO (labeled as Pt-KTO/5% rGO) composite catalysts were thoroughly characterized by XRD, FT-IR, Raman, XPS, FE-SEM with EDS, HR-TEM, UV-DRS, and PL analyses. The photocatalytic activity of Pt-KTO/5% rGO was remarkable compared to bare KTO and other rGO-supported catalysts. Pt-KTO/5% rGO composite photocatalyst showed enhanced photocatalytic degradation of 97%, 99%, & 99% for CR, MB & TC at 180, 120 and 180 min, respectively. Mineralization of dyes (TOC of 85%, 87%, and COD of 76%, 79% for CR, MB) and antibiotics (TOC of 78% and COD of 69% for TC) was confirmed by chemical oxygen demand (COD) and total organic carbon (TOC) measurements. The Pt-KTO/5% rGO catalyst showing the highest activity also demonstrates recyclability. A suitable photocatalytic reaction mechanism has been proposed for the higher activity of Pt-KTO/5% rGO composite catalyst under visible light irradiation.

Could bacteriophages be used as a novel pest management tool to control gut endosymbionts in Western Flower Thrips?

Several species of Thysanoptera are considered agricultural pests, especially Frankliniella occidentalis (Western Flower Thrips, WFT). The damage WFT causes to plants can either be direct by feeding and oviposition activity or indirect by WFT serving as vectors for several plant viruses, bacteria, and fungi. Properties of WFT like polyphagy, cryptic lifestyle, fast reproduction and the ability to quickly develop insecticide resistance make them difficult to control. Alternative pest management tactics are thus in high demand. Potential targets are the facultative or obligate endosymbionts that affect the life history parameters of WFT. The known endosymbionts of WFT: BFo1/BFo2 (B = bacteria, Fo = Frankliniella occidentalis, number indicate different types) positively influence their fertility and development. Elimination of these endosymbionts could result in a decreased individual reproduction level and decreased population growth. Bacteriophages specific against BFo1/BFo2 were isolated and characterized and were applied in a no-choice feeding assay to female WFT. Antibiotic and sugar solutions served as controls. Phage-treated thrips showed no significant difference in survival, fecundity, oviposition rate or developmental time of the progeny compared to the control, whereas antibiotic treatment caused a decline in survival, fecundity, and oviposition rate, but not in the developmental time of offspring. The results showed a rapid decline of the bacteriophage concentration in the feed solution, which the WFT salivary gland products might cause. The complex relationship between thrips, endosymbionts and bacteriophages, needs further attention before bacteriophages could be used as potential management tools.

Incisional Wound Irrigation for the Prevention of Surgical Site Infection

Surgical site infections (SSIs) are common postoperative complications and associated with significant morbidity, mortality, and costs. Prophylactic intraoperative incisional wound irrigation is used to reduce the risk of SSIs, and there is great variation in the type of irrigation solutions and their use. To compare the outcomes of different types of incisional prophylactic intraoperative incisional wound irrigation for the prevention of SSIs in all types of surgery. PubMed, Embase, CENTRAL, and CINAHL databases were searched up to June 12, 2023. Included in this study were randomized clinical trials (RCTs) comparing incisional prophylactic intraoperative incisional wound irrigation with no irrigation or comparing irrigation using different types of solutions, with SSI as a reported outcome. Studies investigating intracavity lavage were excluded. This systematic review and network meta-analysis is reported in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis statement. Two reviewers independently extracted the data and assessed the risk of bias within individual RCTs using the Cochrane Risk of Bias 2 tool and the certainty of evidence using the Grading of Recommendations, Assessment, Development, and Evaluation framework. A frequentist network meta-analysis was conducted, and relative risks (RRs) with corresponding 95% CIs were reported. The primary study outcome was SSI. A total of 1587 articles were identified, of which 41 RCTs were included in the systematic review, with 17 188 patients reporting 1328 SSIs, resulting in an overall incidence of 7.7%. Compared with no irrigation, antiseptic solutions (RR, 0.60; 95% CI, 0.44-0.81; high level of certainty) and antibiotic solutions (RR, 0.46; 95% CI, 0.29-0.73; low level of certainty) were associated with a beneficial reduction in SSIs. Saline irrigation showed no statistically significant difference compared with no irrigation (RR, 0.83; 95% CI, 0.63-1.09; moderate level of certainty). This systematic review and network meta-analysis found high-certainty evidence that prophylactic intraoperative incisional wound irrigation with antiseptic solutions was associated with a reduction in SSIs. It is suggested that the use of antibiotic wound irrigation be avoided due to the inferior certainty of evidence for its outcome and global antimicrobial resistance concerns.

Antibiotics administered as continuous intravenous infusion over 24 hours by elastomeric devices to patients treated at home: a study of infusion efficiency

AbstractBackgroundElastomeric infusion devices or ‘Infusors’ are commonly used to administer 24‐h continuous intravenous infusions to hospital patients at home, a service which can increase hospital capacity.AimThis study sought to determine Infusor efficiency by measuring infusion lengths administered by Infusors to patients in the community setting and reviewing any impacting factors on varying infusion rates, if observed.MethodPatients and nurses completed data collection forms daily over a 12‐month period. The following information was recorded: time Infusor attached to patient, time Infusor emptied, Infusor ‘empty’ or ‘not empty’ when removed, volume of antibiotic solution remaining, Infusor storage details, antibiotic solution and dose, indication for treatment, and date (season). Statistical analyses was conducted using Stata. Data were analysed using descriptive statistics, including median and range for continuous variables, and frequency counts and percentages for categorical variables. Ethical approval was granted by Northern Sydney Local Health District (NSLHD) Research Office (Reference no: RESP/14/184), the Human Research Ethics Committee (HREC) (Reference no: LNR/14/HAWKE/265) and the study conforms to the Australian National Statement on Ethical Conduct in Human Research. Informed consent was obtained from all participants via a study information leaflet that was provided with the patient questionnaire and patients were informed that their participation in the study was optional. Patients indicated their consent by completing the data collection form for each day of treatment.ResultsA significant number of Infusors (27%) emptied outside the expected infusion duration of 24 h ± 10% (21.6–26.4 h) and Infusors were removed ‘not empty’ when the nurse visited >24 h on 35% of occasions. Infusors were more likely to empty >24 h if they contained piperacillin‐tazobactam 13.5 g (predicted probability = 1.0), in winter (predicted probability = 0.83), and in cooler overnight storage locations (predicted probability = 0.64). Infusors were more likely to empty <24 h if they contained vancomycin (predicted probability = 0.12).ConclusionInfusors delivering 24‐h continuous intravenous infusions in the home setting may empty at unpredictable times and may be affected by temperature or solutions with varying doses. Outpatient parenteral antimicrobial therapy clinicians should be aware of possible unfinished infusions from Infusors.

Antibiotic lock solutions as adjunct therapy for catheter-related blood stream infections in pediatric hemodialysis patients.

The predominant use of intravenous catheters as primary access type in the pediatric hemodialysis population is associated with an increased risk of catheter related blood stream infections. While strict adherence to catheter placement and long-term care guidelines have helped to decrease the incidence of these infections, blood stream infections remain an infection burden in pediatric patients with long term hemodialysis catheters. The formation of biofilms on the surfaces of these catheters has been shown to be a source of microbes causing blood stream infections. One of the strategies for preventing bacterial colonization, inhibiting microbial multiplication, and suppressing the seeding of these microbes from biofilms upon maturation, has been the use of antibiotic-based lock solutions in-between dialysis treatments. Although clinical guidelines for the use of antibiotic lock solutions are yet to be developed, available evidence suggests a beneficial role of antibiotic lock solutions in the management of catheter related blood stream infections. Additionally, a clear understanding of how biofilms are formed and their role in the pathogenesis of catheter related bloodstream infection will facilitate the development of solutions that can prevent biofilm formation and inhibit their multiplication, maturation and seeding into the bloodstream.

Modeling of Mass Transfer and Reaction Kinetics in ZnO Nanoparticle Micro-Reactor Systems for AMX and DOX Degradation

This study aims to model the coupled phenomena of photocatalytic reaction and mass transfer in the degradation of Amoxicillin (AMX) and Doxycycline (DOX) using Zinc oxide (ZnO) nanoparticles within microreactor systems. The objective is to gain a comprehensive understanding of the dynamic interaction between the photocatalytic degradation kinetics and the mass transfer processes to optimize the conditions for efficient antibiotic removal from contaminated water. This involves characterizing the reaction kinetics via the Langmuir-Hinshelwood model, estimating the mass transfer coefficients, and analyzing the effects of axial dispersion to ensure the accurate determination of intrinsic kinetic constants and minimize mass transfer limitations. This study used a syringe pump to ensure a consistent flow of antibiotic solution into the microreactor. The results indicate that AMX reaches adsorption equilibrium more rapidly than DOX, corresponding to its faster photocatalytic degradation kinetics and higher final conversion rate (89% for AMX, 86% for DOX). The mass transfer coefficient (kd) was estimated using the Sherwood number, derived from three different models, with the constant Sherwood model best fitting the R1 microreactor data. An analysis of the Damköhler number (DaII) indicates that high flow rates minimize mass transfer limitations in the R1 microreactor, allowing the determination of near-intrinsic kinetic constants. On the contrary, at low flow rates, kinetic constants are apparent as a result of mass-transfer limitations. The study concludes that higher flow rates (≥ 10 mL/h) in the R1 microreactor are preferable to approach intrinsic kinetics and reduce mass transfer limitations during photocatalytic degradation of antibiotics. These findings underscore the potential of ZnO-based oxidation processes in treating antibiotic-contaminated water with optimized conditions, providing a pathway for efficient and sustainable wastewater treatment.

Contact-electro-catalysis for degradation of trace antibiotics in wastewater

Antibiotic contamination of water bodies is a major concern in environmental management. Despite their superior efficacy in antibiotic removal, advanced oxidation processes (AOPs) have yet to be widely adopted owing to their substantial cost associated with the large amounts of chemicals required. In this study, a novel contact-electro-catalysis (CEC) process with reusable polytetrafluoroethylene (PTFE) particles as a trigger catalyst was applied to replace traditional AOPs, and it was able to degrade three typical antibiotics (sulfamethoxazole [SMX], ciprofloxacin [CIP], and tetracycline [TET]) in the secondary effluent of a wastewater treatment plant for the first time. The PTFE particles at a concentration of 0.8 g/L were introduced into antibiotic solutions with an antibiotic concentration of 1 mg/L, and the removal rates of SMX, CIP, and TET after a 90-min operation time reached 50 %, 80 %, and 90 %, respectively. Our findings revealed that the CEC reaction generated ●OH and 1O2, contributing substantially to antibiotic degradation. Thus, new reactive oxygen species generation mechanisms during the CEC process were proposed. Furthermore, we fabricated polyvinylidene fluoride nanofiber membranes using electrospinning to recycle PTFE particles, where a nearly 100 % recovery rate through dead-end filtration was realized with a high water flux of 7.5 m3/(m2∙h). The results of this study provide a promising approach for antibiotic removal from wastewater.

A Histological Evaluation of the Efficiency of Using Periprosthetic Autologous Fat to Prevent Capsular Contracture Compared to Other Known Methods-An Experimental Study.

Capsular contracture remains a common complication in silicone breast implantation. The etiology, formation mechanisms, predisposing and favoring factors are still subjects of research. This study aims to demonstrate the effectiveness of using autologous fat introduced periprosthetically in preventing capsular contracture compared to other known methods: antibiotics and corticosteroids. A cohort of 80 Wistar rats was included in the study, divided into four subgroups. All subjects received a silicone implant, implanted in a pocket created along the abdominal midline. The first subgroup served as the control group, with subjects having the implant placed without any treatment. For the second and third subgroups, the implants were treated with an antibiotic solution and intramuscular injections of dexamethasone, respectively. The subjects in the last subgroup received centrifuged autologous fat introduced periprosthetically. The subgroup with autologous fat exhibited a significantly smaller capsule thickness, which was poorly represented, with a smooth surface. The use of autologous fat for treating silicone prosthesis was linked with the lack of acute inflammation around the prosthetic site. Autologous fat helps to minimize the "non-self" reaction, which results in the development of a periprosthetic capsule consisting of mature collagen fibers interspersed with adipocytes.

Seasonal variation in contamination and browning of Acacia nilotica nodal explants in vitro

Major problems encountered in establishing axenic cultures are contamination and browning of explants and media. Contamination is initiated in explants excised from soil-borne tissues or adult trees. Several sterilizing agents (Tween 20, chlorine water, silver nitrate, mercuric chloride, etc.) or a wash in antibiotic solution are recommended to reduce this. . Establishing in vitro cultures of Acacia nilotica old tree nodal explants was a serious problem. The present study was undertaken to create a suitable protocol for in vitro micropropagation of nodal explants of Acacia nilotica subsp. indica. Contamination was controlled by sterilizing the explants by washing in polysan (5%, v/v along with 500mg/l PVP), thorough washing under tap water, 70% alcohol treatment and 0.1% HgCl2 treatment. Plant tissues release phenolic substances through their cut ends, which turn the media dark brown and toxic. Addition of various antioxidants or/and transfer of explants to fresh medium twice or thrice, at a few days’ intervals may overcome the problem. Collection of explants in antioxidant solution and a wash in antioxidant solution prior to inoculation was helpful in reducing the phenolic exudation in the present investigations. The addition of antioxidant (citric acid) to the medium also checked browning to some extent. The rate of infection and browning of explants varied in different seasons, the maximum being during winters and the minimum during summers of 2019. This was inversely related to the morphogenic response of explants i.e. maximum caulogenesis occurred in vitro during July (6.3±1.4 shoots per explant). The in vitro raised shoots showed 100% rooting on 2mg/l IBA augmented Nitsch’s (N) medium.

In vitro Wound Healing Effect of a Siddha Formulation: Gandhaga Thailam

In vitro Wound Healing Effect of a Siddha Formulation: Gandhaga Thailam

The burden of antibiotic resistance of the main microorganisms causing infections in humans - review of the literature.

One of the biggest threats to human well-being and public health is antibiotic resistance. If allowed to spread unchecked, it might become a major health risk and trigger another pandemic. This proves the need to develop antibiotic resistance-related global health solutions that take into consideration microdata from various global locations. Establishing positive social norms, guiding individual and group behavioral habits that support global human health, and ultimately raising public awareness of the need for such action could all have a positive impact. Antibiotic resistance is not just a growing clinical concern but also complicates therapy, making adherence to current guidelines for managing antibiotic resistance extremely difficult. Numerous genetic components have been connected to the development of resistance; some of these components have intricate paths of transfer between microorganisms. Beyond this, the subject of antibiotic resistance is becoming increasingly significant in medical microbiology as new mechanisms underpinning its development are identified. In addition to genetic factors, behaviors such as misdiagnosis, exposure to broad-spectrum antibiotics, and delayed diagnosis contribute to the development of resistance. However, advancements in bioinformatics and DNA sequencing technology have completely transformed the diagnostic sector, enabling real-time identification of the components and causes of antibiotic resistance. This information is crucial for developing effective control and prevention strategies to counter the threat.

(130) Antibiotic Solutions are the Ideal Irrigation Choice for Inflatable Penile Implant Hydrophilic Surfaces

Abstract Introduction Inflatable penile prostheses (IPP) may be colonized by bacteria, which may contribute to clinical infections. Current attempts to reduce bacterial colonization include altered implant surface binding properties and mechanical irrigations with antiseptic solutions. Previous data has shown that prophylactically dipping IPPs in a solution of 2mg/mL vancomycin and 160μg/mL gentamicin (VG) reduces biofilm formation more than chlorhexidine gluconate (CHG) does. However, there is a paucity of data looking at VG as an irrigation step after a prophylactic dip. Objective To determine if VG irrigation of a previously CHG- or NS-dipped IPP decreases bacterial colonization more effectively than irrigation with normal saline. Methods Sterile 8mm diameter IPP pieces (Coloplast, Minneapolis, MN) were split into 4 groups with a minimum of 12 replicates each and submerged for 3 minutes in 3mL of normal saline (NS), 0.05% CHG in sterile water (Irrimax, Lawrenceville, GA), or VG (Sigma-Aldrich, Burlington, MA). Pieces were then rinsed in NS for five seconds, re-submerged in a separate well containing 2mL of NS or VG for 2 minutes, and finally rinsed for five seconds in a large volume of NS. After a 5 sec PBS rinse, pieces were incubated with 1 X 10^5 colony forming units per milliliter (CFU)/mL of methicillin-sensitive Staphylococcus aureus ATCC25923 in trypticase soy broth (TSB) for 48 hours. Samples were then rinsed with PBS and biofilm was removed by shaking in 0.3% Tween-20. Suspended bacteria were serially diluted, plated onto 3M PetriFilms (Maplewood, MN), and then colony counts were recorded. Mann-Whitney U tests were performed to compare outcomes, p<0.05 considered significant. Results Average bacterial counts with 95% CI are plotted in Figure 1. NS controls showed an average of 1.0E7 CFU/mL adherent bacteria. In this study, the introduction of VG to an NS or CHG-coated implant resulted in significant reduction in adherent bacteria; the NS-VG group showed a ~5 log decrease in adherent bacteria (3.0E1, standard deviation=3.3E4, p<0.01), and the CHG-VG group similarly showed a ~5 log decrease in adherent bacteria (4.4E2, standard deviation=6.9E5, p<0.01). Neither treatment group consistently reduced biofilm counts as effectively as the implant pieces incubated in VG as both a prophylactic dip and a rinse solution (~5 log decrease in counts, p<0.01). However, neither treatment group consistently reduced biofilm counts as effectively as the implant pieces incubated in VG as both a prophylactic dip and a rinse solution, also with a ~5 log decrease in adherent bacteria (1.0E2, standard deviation=2.4E2, p<0.01) (Figure 1). Conclusions VG is the ideal choice for both coating the implant prior to implantation and as an intraoperative irrigation solution. However, further studies are needed to compare VG with CHG as mechanical irrigants as opposed to static irrigants. Disclosure Yes, this is sponsored by industry/sponsor: Coloplast (material not financial support). Clarification: Industry funding only - investigator initiated and executed study. Any of the authors act as a consultant, employee or shareholder of an industry for: Coloplast and Boston Scientific.

(045) Comparing Bacterial Presence with the Usage of 0.05% Chlorhexidine Gluconate (CHG) Lavage During Urologic Prosthesis Revision Surgery: Update on Results

Abstract Introduction An Inflatable Penile Prosthesis (IPP) is third line surgical therapy for refractory ED. An IPP uses a fluid-filled reservoir with a pump and valve system to activate inflation and deflation. Although penile implants achieve the highest success rates in ED treatment, the possible presence of bacteria on the device itself or bacteria exposure accumulated during the surgery increases the risk of progression into biofilm or infection. Biofilm complicates the bacteria eradication effort and can ultimately jeopardize the implant. Often, the only way to treat the post-op infection is with implant removal. Currently, many preventative measures, such as different perioperative irrigations, are being studied to reduce the number of penile implant infections. Research has shown that irrigation at the time of both infectious and noninfectious surgery lowers subsequent infection rates. Different solutions like saline, antibiotic, betadine, and hydrogen peroxide solutions have been considered. Recently, orthopedic literature has shown that 0.05% Chlorhexidine Gluconate (CHG) lavage has effectively reduced biofilm in artificial joint procedures. Objective The purpose of this study is to determine the effectiveness of CHG lavage on bacterial presence in IPP implant spaces. We hypothesize that CHG will lower bacterial presence after washing out implant spaces at time of replacement for infections and mechanical failure. Methods We aimed to investigate a series of patients who underwent revision IPP surgery while utilizing FDA-approved CHG lavage for irrigation and compared the bacterial presence before and after CHG washout. Additionally, Next Generation Sequencing (NGS), a DNA sequencing technology, was used to further identify the pathogens involved. Results Between 2021 to 2023, 48 patients underwent IPP revision surgery for either infectious or noninfectious reasons. During the operation, a section of the capsule tissue surrounding the implanted IPP pump was first taken and sent for cultures. NGS studies were also sent out. After the implant removal, all implant spaces were irrigated and washed out with CHG (two 450-mL bottles). A second tissue culture was then obtained post washout. Of the 48 patients, there were 4 positive bacterial cultures before washout and 13 positive NGS results. Results of the post CHG washout cultures were all negative. Conclusions It appears that irrigation with 0.05% Chlorhexidine Gluconate solution is an effective antiseptic that can decrease bacterial presence, and hopefully reduce IPP infections. Disclosure Any of the authors act as a consultant, employee or shareholder of an industry for: Microgen Dx, Irrisept.

Pyolytics as a product of the physical–chemical repurposing of antiseptics and an alternative to larval therapy for chronic wounds

The traditional treatment of chronic wounds involves daily cleansing of the wound surface from purulent necrotic masses using mechanical and medicinal methods, accompanied by regular replacement of wound dressing. In this case, medicinal wound cleansing lasts 10–15 mins from the time of replacement of the old wound dressing with the new one. According to established practice, medicinal sanitation of infected and purulent wounds during dressing involves irrigation of the wound surface with cleansing solutions, antiseptics, and/or antibiotics. In severe cases, the above therapy is supplemented with live larvae of the necrophage fly, which are injected into purulent necrotic masses and left in them under wound dressing until wounds are completely cleansed from pus. Nevertheless, the generally accepted course of treatment of chronic wounds remains ineffective. The use of pyolytics and their supplementation with wound dressings in the form of warm wet compresses, which create a local greenhouse effect in wounds, was reported to accelerate the healing of chronic wounds. Pyolytics are a group of antiseptics developed in Russia. They are warm alkaline solutions of hydrogen peroxide; when they interact with purulent necrotic masses, these solutions dissolve very quickly and foam them. Because of the interaction with pyolytics, thick purulent masses immediately turn into fluffy oxygenated foam. Pyolytics have been developed because of the physicochemical repurposing of aqueous solutions of sodium hydrogen carbonate and hydrogen peroxide. To accelerate the healing of chronic wounds, a recommendation was to irrigate the surface of chronic wounds with 3% hydrogen peroxide and 2–10% sodium bicarbonate solutions, heated to 37–45°C, which have alkaline activity at pH 8.4–8.5 and are enriched with dissolved carbon dioxide or oxygen (due to excess pressure of 0.2 atm). This study presented the importance of treating chronic wounds using politics and treatment outcomes using pyolytics along with warm moist dressing compresses, demonstrating a wound-healing effect. Consequently, physical and chemical reprofiling of antiseptics may make them effective pyolytics, and the combination of pyolytics with warm wound dressings such as warm moist compresses, which create a local greenhouse effect on wounds, accelerates the healing of chronic wounds.

ABO Blood System: Biosynthesis of Agglutinogenic Alkaline and Non-Agglutinogenic Acid Glycotopes of A and B Antigens at Different pHs of the Culture Medium.

The biosynthesis of agglutinogenic and adsorbing groups A and B glycotopes of the erythrocyte's membrane is mediated by the activity of specific glycosyltransferases. This study aimed to assess the nature of the biosynthesis of A and B antigenic glycotopes, depending on the pH of the medium during the cultivation of erythrocytes, and the antigenic (transferase) characteristics of the donor serum of the other group. Monoclonal antibodies (Mabs) were obtained from IGBRL under Program IV of the International Workshop on Monoclonal Antibodies and Red Blood Cell Antigens. Biosynthesis was performed using erythrocytes, fresh serum, medium 199, and an antibiotic solution. The agglutinogenic characteristics of 11 out of 33 samples changed by the end of the cultivation period due to the acquisition of additional agglutinogen corresponding to the donor serum. None of the samples lost their inherent agglutinogen due to its absence in the donor serum. Four of six samples of O(I) erythrocytes acquired the ability to be agglutinated by anti-A reagents, especially by the polyclonal anti-A, and the manifestation of agglutination depended on the reaction time. Two of the three samples with initial A(II) agglutinogenic specificity added to the donor serum with Bc'+ characteristic of the erythrocytes acquired this characteristic. However, none of the five A(II)Ac'+ samples cultured in the serum of Ac'-O(I)Ac'-Bc'+ and O(I)Ac'-Bc'- donors lost their inherent earlier Ac'+ characteristic. The investigation of the inhibitory ability of alkaline and acidic glycoconjugates isolated from membranes revealed that alkaline Alp-00 and Alp-1 glycotopes isolated from glycolipids had the highest inhibitory activity, and the degree of inhibition of polyclonal anti-A antibodies was even higher than that of monovalent BRIC-131. This study showed the possibility of the biosynthesis of specific non-agglutinogenic A and B glycotopes under the influence of a different group's serum as a source of the corresponding transferase.

Cryptocarya moschata extract decreases single and mixed biofilms on acrylic resins.

This study proposed to assess the effect of Cryptocarya moschata extract on single and mixed biofilms formed on denture base and reline acrylic resin. Single and mixed biofilms of Candida albicans and Streptococcus mutans were formed on the samples and treated with C. moschata extract; Nystatin solution at 100,000 IU/mL or Penicillin antibiotic solution at 100,000 IU/mL; or PBS solution. Antimicrobial activity was analyzed by counting colony-forming units, metabolism assay, assessment of protein components of the biofilm matrix, and of cell viability using confocal laser scanning microscopy (CLSM). Data were submitted to ANOVA and Tukey's post-test (α = 0.05). Cryptocarya moschata extract reduced cell viability of C. albicans and S. mutans single and mixed biofilms formed on samples. For all types of biofilms in the C. moschata group, there was a log reduction of the biofilm, proven by the Alamar Blue assay. Analyzing the extracellular matrix protein components, groups treated with the extract exhibited a lower level of fluorescence compared to the PBS groups. Reduction in thickness biofilm and viable cells was perceptible in the C. moschata group when assessing through CLSM. Cryptocarya moschata extract reduced the single and mixed biofilms of C. albicans and S. mutans on acrylic resins.

Synthesis of pure and Cd-doped hydroxyapatite for the photo-catalytic degradation of Amoxicillin and Ciprofloxacin: Crystallographic characterization using XRD

Nanosized hydroxyapatite (HAp) is considered an elementary material for artificial bone tissue engineering and the applications are expanding and one of them is the photocatalytic degradation of environmentally hazardous pollutants. In this research, HAp nanoparticles, in pure form and doped with cadmium (Cd), were synthesized using a conventional wet-chemical precipitation method. Additionally, the synthesized samples such as pure and Cd-doped HAp were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and UV–Vis spectroscopy. Moreover, the XRD analysis allowed for a detailed estimation of the crystallite size, assisting in their tailoring of increased photocatalytic activity. The crystallite sizes of the nano-crystallite Hap were evaluated using the Scherrer Method (SM), Williamson-Hall Method (WHM), Size-Strain Plot (SSP), Halder-Wagner Method (HWM), and Sahadat-Scherrer Method (SSM). The models confirmed the formation of nano crystallite hydroxyapatite in pure and doped form. Cadmium doping in HAp showed great photocatalytic activity for example at 150 min, 31 % amoxicillin and 52 % ciprofloxacin were degraded upon 1.5 wt % Cd doping in HAp crystal. The research demonstrated that doping Cd in HAp crystal improved photocatalytic activity towards antibiotic solution (ciprofloxacin and amoxicillin) under all test circumstances.