Soluble Products Research Articles

Anti-Fibrotic Potential of Tomentosenol A, a Constituent of Cerumen from the Australian Native Stingless Bee, Tetragonula carbonaria.

Bioactivity-guided fractionation was used to isolate two compounds, tomentosenol A (1) and torellianone A (2), from a cerumen extract from Tetragonula carbonaria. The anti-fibrotic activity of these compounds was examined using human cultured neonatal foreskin fibroblasts (NFF) and immortalised keratinocytes (HaCaTs). Tomentosenol A (1), inhibited NFF and HaCaT cell proliferation and prevented NFF and HaCaT scratch wound repopulation at 12.5–25 µM concentrations. These inhibitory effects were associated with reduced cell viability, determined by tetrazolium dye (MTT) and sulforhodamine B (SRB) assays. Compound 1 further inhibited transforming growth factor-β1 (TGF-β1)-stimulated, NFF-myofibroblast differentiation and soluble collagen production; and was an effective scavenger of the model oxidant, 2,2-diphenyl-1-picrylhydrazyl (DPPH·), with an EC50 value of 44.7 ± 3.1 µM. These findings reveal significant anti-fibrotic potential for cerumen-derived tomentosenol A (1).

Effects of pharmaceuticals on the fouling propensity of membrane bioreactor: Case of iodinated X-ray contrast media

Membrane bioreactor (MBR) has shown promising results in the abatement of pharmaceuticals, however, its fouling propensity has continued to put challenges in its wide-scale applicability. The present study is the first study that investigated the MBR fouling behaviour in the presence of iodinated X-ray contrast media (ICM). The study found that ICM aggravated the fouling issue by reducing the filtration cycle by 21 % and enhancing the production of key foulants- extracellular polymeric substances (EPS) and soluble microbial products (SMP). Size exclusion chromatography (SEC) results revealed the presence of a higher molecular weight (MW) organics in the EPS in ICM dosed phase. Also, excitation-emission matrix (EEM) contours of EPS showed that the exposure to ICM seemed to stimulate the secretion of aromatic proteins comprising tryptophan (28.7 %) and tyrosine (22.07 %) compared to the non-ICM dosed phase (25.10 % for tryptophan and 20.39 % for tyrosine). The floc size of the sludge was found to increase to 67.6 ± 10.2 µm from 22.4 ± 4.8 µm in the presence of ICM as a result of increased EPS content. Further, ICM addition resulted in a slight reduction of the sludge microbial activity (∼10 %). ICM also changed the microbial dynamics of the sludge by enriching the abundance of biofilm forming phyla such as Bacteroidetes and TM7. The findings of this study provide fundamental insights into the fouling propensity of MBR in presence of ICM, and highlight that the changes caused in the EPS secretion profile would be responsible for enhanced fouling.

Characterization, Transformation, and Bioavailability of Dissolved Organic Nitrogen in Biofilters Treating Domestic Onsite Wastewater

This study investigated the transformation, bioavailability, and seasonal changes of dissolved organic nitrogen (DON) in nitrogen removing biofilters (NRBs) treating domestic onsite wastewater. The results demonstrated that NRBs were able to remove 92.6–94.3% DON from septic tank effluent with low effluent DON concentrations (0–5.8 mg N L–1), comparable to the levels detected in the tertiary municipal wastewater treatment plant. Both hydrophilic and hydrophobic DON were efficiently removed by the sand filters, while additional hydrophilic DON (0.8–1.8 mg N L–1) was produced in the lignocellulose/sand layer due to soluble microbial product formation. Seasonal change (i.e., temperature variation) did not impact the effluent DON composition or its removal efficiency by NRBs, while higher concentrations of hydrophilic DON (1.7–2.5 mg N L–1) were detected in the final effluent during the summer months (16.4–22.9 °C). DON in the NRB effluent had a higher potential to stimulate algal growth than other nitrogen forms (NH4+ and NOx–), confirmed by higher chlorophyll-a concentrations observed in the bioassay tests. The results suggest the DON levels in the final effluent of an onsite wastewater treatment system shall also be considered in onsite wastewater quality management and the effluent discharge regulations.

Dynamic Culture of Mesenchymal Stromal/Stem Cell Spheroids and Secretion of Paracrine Factors.

In recent years, conditioned medium (CM) obtained from the culture of mesenchymal stromal/stem cells (MSCs) has been shown to effectively promote tissue repair and modulate the immune response in vitro and in different animal models, with potential for application in regenerative medicine. Using CM offers multiple advantages over the implantation of MSCs themselves: 1) simpler storage, transport, and preservation requirements, 2) avoidance of the inherent risks of cell transplantation, and 3) potential application as a ready-to-go biologic product. For these reasons, a large amount of MSCs research has focused on the characterization of the obtained CM, including soluble trophic factors and vesicles, preconditioning strategies for enhancing paracrine secretion, such as hypoxia, a three-dimensional (3D) environment, and biochemical stimuli, and potential clinical applications. In vitro preconditioning strategies can increase the viability, proliferation, and paracrine properties of MSCs and therefore improve the therapeutic potential of the cells and their derived products. Specifically, dynamic cultivation conditions, such as fluid flow and 3D aggregate culture, substantially impact cellular behaviour. Increased levels of growth factors and cytokines were observed in 3D cultures of MSC grown on orbital or rotatory shaking platforms, in stirred systems, such as spinner flasks or stirred tank reactors, and in microgravity bioreactors. However, only a few studies have established dynamic culture conditions and protocols for 3D aggregate cultivation of MSCs as a scalable and reproducible strategy for CM production. This review summarizes significant advances into the upstream processing, mainly the dynamic generation and cultivation of MSC aggregates, for de CM manufacture and focuses on the standardization of the soluble factor production.

Mechanisms of polystyrene microplastic degradation by the microbially driven Fenton reaction

Natural hydroxyl radical (·OH) production, which partially occurs through the microbially driven Fenton reaction, can enhance the degradation of polystyrene microplastics (PS-MPs). However, ·OH causes damage to microorganisms, which might in turn restrain the microbially driven Fenton reaction. Thus, whether PS-MPs can be continuously degraded by the microbially driven Fenton reaction and how they are degraded are still unknown. A pure-culture system using Shewanella putrefaciens 200 was set up to explore the effect and mechanism of microbially driven Fenton reaction on PS-MP degradation. In a 14-day operation, ·OH produced by the microbially driven Fenton reaction could degrade PS-MPs with a weight loss of 6.1 ± 0.6% and an O/C ratio of 0.6 (v.s. 0.6 ± 0.1% and 0.1, respectively, in the ·OH quenched group). Benzene ring derivatives such as 2-isopropyl-5-methyl-1-heptanol and nonahexacontanoic acid were the main soluble products of PS-MP degradation. The ·OH-induced oxidative damage on microorganisms did not affect ·OH production significantly when there was timely replenishment of organic carbon sources to promote reproduction of microorganisms. Thus, PS-MPs can be continuously degraded by microbially driven Fenton reactions in natural alternating anaerobic-aerobic environments. This study also provides a new microbial technique for MP degradation that is different from previous technologies based on microbial plastic-degrading enzymes.

Effects of alteration on shear characteristics of compacted Ca-bentonite immersed in alkaline solutions

This study evaluated the shear characteristics of compacted Ca-bentonite immersed in 0.1 mol/L of NaOH, KOH, and KOH–NaOH and 0.005 mol/L of Ca(OH)2 at 40℃ over a maximum period of 1710 days. Triaxial compression tests were performed on the immersed specimens, and the mineral composition, mean layer charge, leachable cations, and microstructure were investigated. The dissolution of cristobalite was significant at high pH levels, whereas phillipsite was precipitated in the specimens immersed in the NaOH and NaOH–KOH solutions. The amount of leachable cations increased substantially, indicating that soluble secondary products (non-crystalline phase) were present in the specimens, as was proven by the observation of gel-like products comprised of Ca and Si on the microphotograph. An increase and decrease in the maximum deviator stress occurred as a result of the dissolution and precipitation. A structural parameter was proposed in this study by assuming the contribution of the secondary products to the cementation of the soil skeleton. This provided a series state transition of the compacted bentonite, where the maximum deviator stress increased with the cementation of the non-crystalline secondary phase. However, the progressing dissolution of the primary minerals decreased the dry density, thereby loosening the cemented structure and reducing the maximum deviator stress.

Harsh temperature induces Microcystis aeruginosa growth enhancement and water deterioration during vernalization

Cyanobacterial blooms are seasonal phenomena in eutrophic water. Cyanobacteria grow fast in the warm spring/summer while disappearing in cold autumn/winter. The temperature change induces algal vernalization. However, whether vernalization affects cyanobacterial blooms, and the regulatory signaling mechanisms are unclear. This study used Microcystis aeruginosa as the model cyanobacteria, and 4 °C and 10 °C as the low-temperature stimulation to explore the cell growth, metabolites, and signaling pathways in cyanobacteria vernalization. Low temperatures induced M. aeruginosa vernalization; the growth rate and cell density increased by 35±4% and 33±2%. Vernalization influenced peptidoglycan synthesis and cell permeability. Soluble microbial products (SMPs) in water increased by 109±5%, resulting in water deterioration. Polysaccharides were the predominant SMPs during the initial term of vernalization. Tryptophan protein-like & humic acid-like substances became the main increased SMPs in the middle-later period of vernalization. Harsh temperatures triggered quorum sensing and two-component system. Signaling sensing systems upregulated photosynthesis, glycolysis, TCA cycle, oxidative phosphorylation, and DNA replication, enhancing M. aeruginosa growth and metabolism during vernalization. This study verified that low temperature stimulates cyanobacteria growth and metabolism, and vernalization possibly aggravates cyanobacterial blooms and water deterioration. It provides new insights into the mechanism of seasonal cyanobacterial blooms and the pivotal role of signaling regulation.

Antigens from the parasitic nematode Trichuris suis induce metabolic reprogramming and trained immunity to constrain inflammatory responses in macrophages.

Regulation of macrophage (Mɸ) function can maintain tissue homeostasis and control inflammation. Parasitic worms (helminths) are potent modulators of host immune and inflammatory responses. They have evolved various strategies to promote immunosuppression, including redirecting phagocytic cells toward a regulatory phenotype. Although soluble products from the whipworm Trichuris suis (TSPs) have shown significant effects on Mɸ function, the mechanisms underlying these modulatory effects are still not well understood. In this study, we find that TSPs suppressed inflammatory cytokines (TNF and IL-6) in Mɸs stimulated with a broad panel of TLR agonists, whilst inducing IL-10. Moreover, M1 markers such as MHCII, CD86, iNOS, and TNF were downregulated in TSP-treated Mɸs, without polarizing them towards an M2-like phenotype. We showed that TSPs could establish a suppressed activation state of Mɸs lasting at least for 72h, indicating an anti-inflammatory innate training. Moreover, we found that TSPs, via repression of intracellular TNF generation, decreased its secretion rather than interfering with the release of surface-bound TNF. Metabolic analysis showed that TSPs promote oxidative phosphorylation (OXPHOS) without affecting glycolytic rate. Collectively, these findings expand our knowledge on helminth-induced immune modulation and support future investigations into the anti-inflammatory properties of TSPs for therapeutic purposes.

Comparison of high-concentration azo dye removal by long HRT in MSBRs' bioaugmented with GAC and sponge media.

The present study assessed the performance and fouling of adding granular activated carbon (GAC) and sponge (BioCube), as two different media, to a membrane sequencing batch reactor (MSBR) system in wastewater treatment containing Acid Red 18 (AR 18). Anaerobic phase, aerobic phase, and hydraulic retention times (HRTs) of 24 h, 12 h, and 72 h were considered for 500 mg/L AR 18 removal at a sludge retention time (SRT) of 20 days by separately adding up to 35% BioCube volume and 8 g/L GAC to the reactors. Based on the kinetic study, 63 mg/L (87% removal) and 115 mg/L (77% removal) remaining dye were reported in the GAC and BioCube membrane sequencing batch reactors (GAC-MSBR and BioCube-MSBR), respectively. A gradual oxidation-reduction potential decline toward -416 mV confirmed better dye removal in GAC-MSBR than BioCube-MSBR, observing a sudden drop to -354 mV. The morphology can explain better biological treatment in GAC-MSBR in addition to the adsorption process. Soluble microbial products (SMPs) of 126.92 mg/L and 395.18 mg/L were obtained for GAC-MSBR and BioCube-MSBR, respectively. Chemical oxygen demand (COD) and SMP indicated that the GAC-MSBR water quality is better than that of the other reactor.

Time-lagged interspecies interactions prevail during biofilm development in moving bed biofilm reactor.

Clarifying the essential succession dynamics of interspecies interactions during biofilm development is crucial for the regulation and application of biofilm-based processes. In this study, regular and time-series phylogenetic molecular ecological networkswere constructed to investigate ordinary and time-lagged interspecies interactions during biofilm development in a moving bed biofilm reactor.Positive interactions dominated both regular (89.78%) and time-series (77.04%) ecological networks, suggesting that extensive cooperative behaviors facilitated biofilm development. The pronounced directional interactions (72.52%) in the time-series network further indicated that time-lagged interspecies interactions prevailed in the biofilm development process. Specifically, the proportion of directional negative interactions was higher than that of positive interactions, implying that interspecific competition preferred to be time-lagged. The time-series network revealed that module hubs exhibited extensive time-lagged positive interactions with their neighbors, and most of them exhibited altruistic behaviors. Keystone species possessing more positive interactions were positively correlated with biofilm biomass, NO3 - -N concentrations, and the removal efficiencies of NH4 + -N and chemical oxygen demand. However, keystone species and peripherals that were negatively targeted by their neighbors showed positive correlations with the concentrations of NO2 - -N, polysaccharides, and proteins in the soluble microbial products. The data highlight that the time-series network can provide directional microbial interactions along with the biofilm development process, which would help to predict the tendency of community shifts and propose efficient strategies for the regulation of biofilm-based processes.

New insights into modeling two-step nitrification in activated sludge systems – The effects of initial biomass concentrations, comammox and heterotrophic activities

In this study, the conventional two-step nitrification model was extended with complete ammonia oxidation (comammox) and heterotrophic denitrification on soluble microbial products. The data for model calibration/validation were collected at four long-term washout experiments when the solid retention time (SRT) and hydraulic retention time (HRT) were progressively reduced from 4 d to 1 d, with mixed liquor suspended solids (MLSS) of approximately 2000 mg/L at the start of each trial. A new calibration protocol was proposed by including a systematic calculation of the initial biomass concentrations and microbial relationships as the calibration targets. Moreover, the impact assessment of initial biomass concentrations (X) and maximum growth rates (μ) for ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB), comammox Nitrospira, and heterotrophs on the calibration accuracy were investigated using the response surface methodology (RSM). The RSM results revealed the strongest interaction of XAOB and μAOB on the model calibration accuracy. All the examined model efficiency measures confirmed that the extended model was accurately calibrated and validated. The estimated μ values were as follows: μAOB = 0.38 ± 0.005 d−1, μNOB = 0.20 ± 0.01 d−1, μCMX = 0.20 ± 0.01 d−1, μHET = 1.0 ± 0.03 d−1. For comparison, when using the conventional model, μAOB and μNOB increased respectively by 26 and 15 % (μAOB = 0.48 ± 0.02 d−1 and μNOB = 0.23 ± 0.005 d−1). This study provides better understanding of the effects of the initial biomass composition and the accompanying processes (comammox and heterotrophic denitrification) on modeling two-step nitrification.

The long and winding road: From mouse linkage studies to a novel human therapeutic pathway in type 1 diabetes.

Autoimmunity involves a loss of immune tolerance to self-proteins due to a combination of genetic susceptibility and environmental provocation, which generates autoreactive T and B cells. Genetic susceptibility affects lymphocyte autoreactivity at the level of central tolerance (e.g., defective, or incomplete MHC-mediated negative selection of self-reactive T cells) and peripheral tolerance (e.g., failure of mechanisms to control circulating self-reactive T cells). T regulatory cell (Treg) mediated suppression is essential for controlling peripheral autoreactive T cells. Understanding the genetic control of Treg development and function and Treg interaction with T effector and other immune cells is thus a key goal of autoimmunity research. Herein, we will review immunogenetic control of tolerance in one of the classic models of autoimmunity, the non-obese diabetic (NOD) mouse model of autoimmune Type 1 diabetes (T1D). We review the long (and still evolving) elucidation of how one susceptibility gene, Cd137, (identified originally via linkage studies) affects both the immune response and its regulation in a highly complex fashion. The CD137 (present in both membrane and soluble forms) and the CD137 ligand (CD137L) both signal into a variety of immune cells (bi-directional signaling). The overall outcome of these multitudinous effects (either tolerance or autoimmunity) depends upon the balance between the regulatory signals (predominantly mediated by soluble CD137 via the CD137L pathway) and the effector signals (mediated by both membrane-bound CD137 and CD137L). This immune balance/homeostasis can be decisively affected by genetic (susceptibility vs. resistant alleles) and environmental factors (stimulation of soluble CD137 production). The discovery of the homeostatic immune effect of soluble CD137 on the CD137-CD137L system makes it a promising candidate for immunotherapy to restore tolerance in autoimmune diseases.

Optimizing dose of coagulant and pH values for membrane fouling control in a submerged membrane bioreactor

AbstractBACKGROUNDThis study assessed the impact of using polyaluminum chloride (PAC) and of changing pH values on the removal efficiency and membrane fouling in a submerged membrane bioreactor (MBR) system utilized to treat real pharmaceutical wastewater.RESULTSThe results revealed that sweep coagulation and a neutral pH were suitable for decreasing soluble microbial products (SMP) in the activated sludge, and the sludge filterability performed better with bridging coagulation and an acidic pH (6.5 < pH < 7). In addition, it was found that membrane fouling was greatly alleviated when PAC was added under the optimized conditions to the MBR (PAC concentration = 100 mg L−1 and pH = 7). Further analyses of membrane fouling mechanisms indicated that adding PAC to the MBR increased the biomass floc size and reduced the SMP concentration and the carbohydrate content in the mixed liquor.CONCLUSIONThe detailed analyses of the membranes' cake layers showed that the presence of PAC decreased the amount of fluorescent organic carbons, proteins, and carbohydrates. These changes in sludge characteristics considerably contributed to the reduction of reversible and irreversible membrane fouling. © 2022 Society of Chemical Industry (SCI).

Degradable Polystyrene via the Cleavable Comonomer Approach

Polystyrene (PS) is a major commodity polymer widely used in various applications ranging from packaging to insulation thanks to its low cost, high stiffness, and transparency as well as its relatively high softening temperature. Similarly to all polymers prepared by radical polymerization, PS is constituted of a C–C backbone and thus is not degradable. To confer degradability to such materials, the copolymerization of vinyl monomers with a cyclic monomer that could undergo radical ring-opening is an efficient method to introduce purposely cleavable bonds into the polymer backbone. Dibenzo[c,e]-oxepane-5-thione (DOT) is a cyclic thionolactone monomer known for its efficient copolymerization with acrylate derivatives but so far could not be incorporated into PS backbones. From a theoretical study combining density functional theory (DFT) and kinetic models using the PREDICI software, we showed that the modification of experimental conditions could overcome these limitations and that high molar mass degradable polystyrene (Mw close to 150 000 g·mol–1) could be prepared via statistical insertion of thioester groups into the polymer backbone. This copolymerization process is compatible with conventional free radical polymerization and reversible deactivation radical polymerization (RDRP) techniques such as nitroxide mediated polymerization (NMP). Thanks to favorable reactivity ratios allowing only a few mol % of thioester units to be randomly incorporated, there was no major modification of the thermal and mechanical properties of the PS. The degradation of such PS could be performed in tetrahydrofuran (THF) at room temperature (RT) in 1 h using 1,5,7-triazabicyclo[4.4.0]dec-5-ene (TBD) as a base, leading to oligomers with Mn close to 2000 g·mol–1. We successfully demonstrate further applicability of these copolymerization systems for the phototriggered decomposition of PS in solution as well as the synthesis of cross-linked PS networks degradable into soluble side products.

Ion-Selective Graphene Oxide/Polyvinyl Alcohol Composite Membranes for Rechargeable Alkaline Zinc Manganese Dioxide Batteries

Blocking the zincate ion ([Zn(OH)4]2–) and limiting its reaction with the cathode material are key requirements for improving the energy density and rechargeability of alkaline zinc manganese dioxide (Zn|MnO2) batteries. Zincate is the soluble anode discharge product, which undergoes a side reaction with the reduced cathode material to form a resistive and irreversible spinel material. Such a side reaction prevents deeper discharge of MnO2, reduces the battery’s achievable energy, and prevents it from further cycling. In this article, we report the design and fabrication of an ion-selective graphene oxide/poly(vinyl alcohol) composite membrane and demonstrate its superiority in suppressing zincate ion crossover while minimally impairing the conduction of hydroxyl ions (OH–). With this advanced separator, near full utilization of the MnO2 electrode’s two-electron capacity (∼617 mA h/g-MnO2) with a high areal capacity of 20 mA h/cm2 is reported for both primary and secondary cells. In the primary cell, the energy density is almost doubled, and in the rechargeable cell, a cycle life of 300 cycles is achieved, which is more than 3 times better than the case with no zincate blocking separator.

Optimization of Autohydrolysis of Olive Pomaces to Obtain Bioactive Oligosaccharides: The Effect of Cultivar and Fruit Ripening

The valorisation of agro-industrial residues presents a challenge in obtaining economically sustainable and environmentally friendly industrial processes. Olive pomace is a by-product generated in large quantities, from olive oil extraction. This residue mostly consists of lignocellulosic materials. The aim of this study was to evaluate the potential use of extracted olive pomaces (EOP) obtained from olives with different ripening indexes (RI) and from different cultivars (Cobrançosa; RI = 2.5; 3.3 and 4.7; and Galega Vulgar; RI = 1.8; 2.9 and 4.8), to produce bioactive oligosaccharides from hemicelluloses by autohydrolysis. The hydrothermal treatment conditions were optimized by Response Surface Methodology, following a central composite rotatable design (CCRD), as a function of temperature (T: 142–198 °C) and time (t: 48–132 min), corresponding to severity factor (SF) values from 3.2 to 4.9. For all pomace samples, soluble sugar production was described by concave surfaces as a function of temperature and time. Autohydrolysis with SF equal or higher than 4.0 produced higher sugar yields, with maximum values around 180 g glucose equivalent/kg EOP for SF of 4.7 (190 °C/120 min) or 4.9 (198 °C/90 min). These values were similar for both cultivars and were not dependent on the ripening stage of the olives. Maximum oligosaccharide (OS) yields of 98% were obtained by autohydrolysis with SF of 4.0. The increase in SF to 4.9 resulted in a decrease in OS yield to 86–92%, due to the release of monomeric sugars. The monosaccharides were mostly xylose (55.8–67.7% in Galega; 50.4–69.0% in Cobrançosa liquid phases), and glucose, galactose, arabinose and rhamnose, in smaller quantities. Therefore, the production of bioactive xylo-oligosaccharides (XOS) from olive pomaces mainly depends on the hydrothermal conditions used.

Biological denitrification potential of cellulase-producing Cupriavidus sp. ZY7 and denitrifying Aquabacterium sp. XL4 at low carbon-to-nitrogen ratio: Performance and synergistic properties

This study emphasizes on the cellulase production characteristics of strain ZY7 and its collaboration with nitrate-dependent ferrous oxidizing (NFO) strain XL4 to achieve efficient denitrification at low carbon-to-nitrogen (C/N) ratio. Results indicated that the denitrification efficiency increased from 65.47 to 97.99% at 24 h after co-culture at C/N of 1.0. Three-dimensional fluorescence excitation-emission matrix (3D-EEM) showed significant changes in the intensity of soluble microbial products (SMP), fulvic-like materials, and aromatic proteins after co-culture. Bio-precipitates were characterized by Scanning electron microscope (SEM), Fourier transform infrared spectrometer (FTIR), and X–ray diffraction (XRD), which showed that cellulose structure was disrupted and the metabolites were potential carbon source for denitrification. In addition, cellulase activity suggested that the hydrolysis of β-1,4-glycosidic bonds and oligosaccharides may be the rate-limiting steps in cellulose degradation. This work promoted the understanding of denitrification characteristics of co-culture and expanded the application of cellulose degrading bacteria in sewage treatment.

Two Electrolyte Decomposition Pathways at NMC Electrodes in Lithium-Ion Batteries

Preventing the decomposition reactions of electrolyte solutions is essential for extending the lifetime of lithium-ion batteries. However, the exact mechanism(s) for electrolyte decomposition at the positive electrode, and particularly the soluble decomposition products that form and initiate further reactions at the negative electrode, are still unknown. In this work, a combination of operando gas measurements and solution NMR was used to study decomposition reactions of the electrolyte solution at NMC (LiNixMnyCo1-x-yO2) and LCO (LiCoO2) electrodes. A partially delithiated LFP (LixFePO4) counter electrode was used to selectively identify the products formed through processes at the positive electrode. Based on the detected soluble and gaseous products, two distinct routes with different onset potentials are proposed for the decomposition of the electrolyte solution at NMC electrodes. At low potentials (<80% state-of-charge, SOC), ethylene carbonate (EC) is dehydrogenated to form vinylene carbonate (VC) at the NMC surface, whereas at high potentials (>80% SOC), 1O2 released from the transition metal oxide chemically oxidises the electrolyte solvent (EC) to CO2, CO and H2O. The formation of water via this mechanism was confirmed by reacting 17O-labelled 1O2 with EC and characterising the reaction products via 1H and 17O NMR spectroscopy. The water that is produced initiates secondary reactions, leading to the formation of the various products identified by NMR spectroscopy. Noticeably fewer decomposition products were detected in NMC/graphite cells compared to NMC/LixFePO4 cells, which is ascribed to the consumption of water (from the reaction of 1O2 and EC) at the graphite electrode, preventing secondary decomposition reactions. The insights on electrolyte decomposition mechanisms at the positive electrode, and the consumption of decomposition products at the negative electrode contribute to understanding the origin of capacity loss in NMC/graphite cells, and are hoped to support the development of strategies to mitigate the degradation of NMC-based cells.

Biocrude production by catalytic hydrothermal liquefaction of wood chips using NiMo series catalysts

In this work, hydrothermal liquefaction of wood chips was studied for biocrude production using a mix of Ni–Mo nitrides and carbides. The catalytic materials were synthesized by a temperature-programmed reaction method at 800 °C under a hydrogen atmosphere with nickel loads from 0 to 20 wt%. Lignin contained in the lignocellulosic biomass was successfully release via Kraft process. Hydrothermal liquefaction was carried out in a batch reactor at 320 °C with an initial pressure of 1000 psi of H2. According to characterization results, nanostructured catalysts with a mix of Ni2Mo3N/Mo2C compo4unds were obtained. NiMo series catalysts composition varies with nickel loads. The catalytic activity shows a reduction in the amount of solid products and an increase in the production of gaseous products as a function of the increase Ni loadings on the catalyst. The most optimal production of biocrude was obtained with the NiMo-10 catalyst, since 81.43% of the total product corresponded to water soluble products (WPS) fraction and the oil fraction, while the solids fraction represented the 6.43% of the total product. Hydrothermal liquefaction catalytic processes were selective towards WSP fraction, improving biocrude quality and favoring biocrude conversion into advanced biofuels.

Re-hydrolysis characteristics of alkaline fermentation liquid from waste activated sludge: Feasibility as a carbon source for nitrogen removal

To overcome the issue of substandard nitrogen and phosphorus discharge, resulting from the low carbon/nitrogen (C/N) ratio of municipal wastewater, immobilized filler was used to re-hydrolyze the alkaline fermentation liquid of waste activate sludge (WAS) as a nitrogen removing carbon source. The complex organic matter was further hydrolyzed and its denitrification efficiency as a carbon source for nitrogen removal was improved. Volatile fatty acids increased from 1605 ± 5 mg/L to 2546 ± 37 mg/L after re-hydrolysis of mixed-alkali sludge fermentation liquid, and complex organic matter, such as fulvic acids, humic acids, class I aromatic proteins, and soluble microbial products were hydrolyzed into small-molecule organics. When the re-hydrolysis fermentation liquid (RH-SFL) was used as the carbon source for nitrogen removal, the nitrogen removal effect was close to sodium acetate and the nitrogen removal rate (NRR) was up to 98.5 ± 0.5%. According to the NOX--N removal curve, the reaction rate of RH-SFL was significantly higher than before re-hydrolysis (nRH-SFL) (0.486 > 0.38). This technology can significantly improve the effective organic quality in the alkaline fermentation liquid of WAS, which can be more easily utilized by denitrifying bacteria. This provides a feasible and effective solution for nitrogen removal in municipal wastewater with insufficient carbon.