According to the method as reported previously, a crude fucose-containing polysacchaide was extracted from the brown algae Kjellmaniella crassifolia and the extract was purified by precipitation with 20% (v/v) ethanol in the presence of 0.05M Mgcl2 to remove contaminated soluble alginate. Then, 1% solution of this purified polysaccharide heated at pH2 at 80°C for 1h and dissolved in 0.05M Mgcl2 was fractionated by the addition of ethanol to 50% (v/v) into soluble (B) and insoluble (A) fractions. Both A and B migrated as a single band on cellulose acetae film electrophoresis. They contained fucose, uronic acid (as glucuronic acid) and ester sulfate in the molor ratios of 1:4:2.3 (A), and 1:0.1:1.1 (B) respectively.
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