Water Solubilization Research Articles

True-solution-scale utilization of natural chlorophyll a in aqueous media through cooperative aggregation with phycocyanin

The challenge of applying chlorophyll(Chl) in aqueous media has been a significant obstacle to the diversified development of Chl a-related industries. This study presents the first report on the true-solution-scale utilization of Chl in aqueous media through the construction of chlorophyll a-phycocyanin (Chls-PC) composite nanoparticles. This study determined the optimal conditions for Chls-PC preparation: a composite ratio of 1:25, a solvent ratio of 1:4, and a stirring time of 1 h. Fluorescence spectroscopy, transmission electron microscope, and confocal microscopy confirmed Chl a and PC aggregation. Surface hydrophobicity and contact angle measurements showed that Chls-PC water solubility was similar to PC and much higher than Chl. Infrared spectroscopy, quantum chemical calculations, X-ray photoelectron spectroscopy, and molecular dynamics simulations elucidated the water solubilization mechanism of Chls-PC both experimentally and theoretically. This research provides theoretical guidance for the development and production of water-based products using Chl as a raw material.

An investigation of quantum dot theranostic probes for prostate and leukemia cancer cells using a CdZnSeS QD-based nanoformulation

Anticancer theranostic nanocarriers have the potential to enhance the efficacy of pharmaceutical evaluation of drugs. Semiconductor nanocrystals, also known as quantum dots (QDs), are particularly promising components of drug carrier systems due to their small sizes and robust photoluminescence properties. Herein, bright CdZnSeS quantum dots were synthesized in a single step via the hot injection method. The particles have a quasi-core/shell structure as evident from the high quantum yield (85 %), which decreased to 41 % after water solubilization. These water solubilized QDs were encapsulated into gallic acid / alginate (GA-Alg) matrices to fabricate imaging QDs@mod-PAA/GA-Alg particles with enhanced stability in aqueous media. Cell viability assessments demonstrated that these nanocarriers exhibited viability ranging from 63 % to 83 % across all tested cell lines. Furthermore, the QDs@mod-PAA/GA-Alg particles were loaded with betulinic acid (BA) and ceranib-2 (C2) for in vitro drug release studies against HL-60 leukemia and PC-3 prostate cancer cells. The BA loaded QDs@mod-PAA/GA-Alg had a half-maximal inhibitory concentration (IC50) of 8.76 μg/mL against HL-60 leukemia cells, which is 3-fold lower than that of free BA (IC50 = 26.55 μg/mL). Similar enhancements were observed with nanocarriers loaded with C2 and simultaneously with both BA and C2. Additionally, BA:C2 loaded QDs@mod-PAA/GA-Alg nanocarriers displayed a similar enhancement (IC50 = 3.37 μg/mL compared against IC50 = 11.68 μg/mL for free BA:C2). The C2 loaded QDs@mod-PAA/GA-Alg nanocarriers had an IC50 = 2.24 μg/mL against HL-60 cells. C2 and BA loaded QDs@mod-PAA/GA-Alg NCr had IC50 values of 7.37 μg/mL and 24.55 μg/mL against PC-3 cells, respectively.

Industrial byproduct pine nut skin factorial design optimization for production of subcritical water extracts rich in pectic polysaccharides, xyloglucans, and phenolic compounds by microwave extraction

Pine nut skin (Pinus pinea L.) is a poorly explored industrial byproduct with potential to be utilized as a food ingredient. Subcritical water extraction (SWE), an eco-friendly extraction technique with higher efficiency than hot-water extraction (HWE), was studied to evaluate its suitability in producing extracts rich in soluble fiber and phenolic compounds. For this, a factorial design was developed considering temperature (120–180 °C), time (2–10 min), and mass/volume ratio (1–3 g/60 mL) under microwave irradiation. This design aimed to maximize the extraction of carbohydrates, while achieving the highest content of phenolic compounds and antioxidant activity. SWE produced higher yields (2.6-fold in relation to HWE) of extracts rich in polysaccharides, determined by methylation analysis, oligosaccharides, determined by GC-qMS as alditol acetates, and phenolic compounds, determined by HPLC-ESI-MS. SWE increased the recovery of pectic oligosaccharides (10-fold) and xyloglucans (2-fold), and allowed to recover pectic polysaccharides, type II arabinogalactans and insoluble-bound phenolic compounds. Mono-, oligo- and polysaccharides were not hydrolyzed during in vitro gastrointestinal digestion, showing potential prebiotic functionality. Although phenolic compounds suffered a 23 % (gallic acid equivalents) decrease, phenolic acids and aldehydes were released or conserved upon intestinal phase. These results highlight the potential of PNS valorization as functional food ingredients through the subcritical water solubilization of polysaccharides and phenolic compounds.

Prebiotic levan type fructan from Bacillus subtilis PR-C18 as a potent antibiofilm agent: Structural elucidation and in silico analysis

The global demand for therapeutic prebiotics persuades the quest for novel exopolysaccharides that can retard the growth of pathobionts and healthcare-associated pathogens. In this regard, an exopolysaccharide (3.69 mg/mL) producing strain showing prebiotic and antibiofilm activity was isolated from indigenous pineapple pomace of Tripura and identified as Bacillus subtilis PR-C18. Zymogram analysis revealed EPS PR-C18 was synthesized by levansucrase (∼57 kDa) with a maximal activity of 4.62 U/mg. Chromatography techniques, FTIR, and NMR spectral data revealed the homopolymeric nature of purified EPS with a molecular weight of 3.40 × 104 Da. SEM and rheological study unveiled its microporous structure and shear-thinning effect. Furthermore, EPS PR-C18 showed remarkable emulsification, flocculation, water retention, water solubilization, and antioxidant activity. DSC-TGA data demonstrated its high thermostability and cytotoxicity analysis verified its nontoxic biocompatible nature. In addition, the antibiofilm activity of EPS PR-C18 was validated using molecular docking, molecular simulation, MM-GBSA and PCA studies, which exhibited its strong binding affinity (−20.79 kcal/moL) with PelD, a virulence factor from Pseudomonas aeruginosa. Together, these findings support the future exploitation of EPS PR-C18 as an additive or adjuvant in food and pharmaceutical sectors.

Effects of Astaxanthin Preparation Form on the Efficiency of Egg Yolk Pigmentation in Laying Hens.

This study investigated the effects of the preparation form of astaxanthin on egg yolk pigmentation and egg quality in laying hens. The following four astaxanthin sources were prepared in this study: (1) dried cell powder of Paracoccus carotinifaciens (Panaferd-AX), (2) fine cell powder of P. carotinifaciens (Panaferd-P), (3) astaxanthin oil suspension, and (4) water-soluble astaxanthin powder. These astaxanthin preparations were added to the basal diet at a final concentration of 2 mg/kg and fed to White Leghorn laying hens for 14 days. Although the administration of these astaxanthin preparations did not largely affect egg quality (i.e., egg weight, yolk weight, albumen height, and Haugh unit), feeding significantly improved astaxanthin concentration and yolk color fan score. When water-soluble astaxanthin powder was fed, the yolk astaxanthin concentration and color fan score were most improved, followed by Panaferd-P. These results indicated that astaxanthin pulverization and water solubilization significantly improved its bioavailability in laying hens. Furthermore, although diets rich in (all-E)-astaxanthin were fed to the hens, approximately 30% of astaxanthin was present as the Z-isomers in the egg yolk. These findings may contribute to improving not only the egg quality but the nutritional value of hen eggs.

The role of surface functionalization in quantum dot-based photocatalytic CO2 reduction: balancing efficiency and stability.

Photocatalytic CO2 reduction offers a promising strategy to produce hydrocarbons without reliance on fossil fuels. Visible light-absorbing colloidal nanomaterials composed of earth-abundant metals suspended in aqueous media are particularly attractive owing to their low-cost, ease of separation, and highly modifiable surfaces. The current study explores such a system by employing water-soluble ZnSe quantum dots and a Co-based molecular catalyst. Water solubilization of the quantum dots is achieved with either carboxylate (3-mercaptopropionic acid) or ammonium (2-aminoethanethiol) functionalized ligands to produce nanoparticles with either negatively or positively-charged surfaces. Photocatalysis experiments are performed to compare the effectiveness of these two surface functionalization strategies on CO2 reduction and ultrafast spectroscopy is used to reveal the underlying photoexcited charge dynamics. We find that the positively-charged quantum dots can support sub-picosecond electron transfer to the carboxylate-based molecular catalyst and also produce >30% selectivity for CO and >170 mmolCO gZnSe-1. However, aggregation reduces activity in approximately one day. In contrast, the negatively-charged quantum dots exhibit >10 ps electron transfer and substantially lower CO selectivity, but they are colloidally stable for days. These results highlight the importance of the quantum dot-catalyst interaction for CO2 reduction. Furthermore, multi-dentate catalyst molecules create a trade-off between photocatalytic efficiency from strong interactions and deleterious aggregation of quantum dot-catalyst assemblies.

Water Solubilization and Guest Molecule-Induced Fluorescence Changes of Porous Host Molecules via Complexing with Polysaccharide or Polypeptide.

To detect small aromatic molecules in water, we prepared functional host molecules based on water-soluble N,N'-bis(2-aminobenzophenone)-1,4,5,8-naphthalenetetracarboxylic diimide (1) and a solubilizing agent using a high-speed vibration milling apparatus. The fluorescence response of host 1-solubilizing agent complexes before and after extraction of small aromatic guest molecules was large and the fluorescence maxima were dependent on the small aromatic guest molecules.

The Characteristic Curvature (Cc) of Ionic Surfactants Assessed via Small-Angle X-ray Scattering.

The characteristic curvature (Cc), within the hydrophilic-lipophilic difference + net (Hn) - average (Ha) curvature (HLD-NAC) framework, is the dimensionless net curvature, -Hn·L (L is the surfactant's tail length parameter), that a surfactant acquires at the characteristic condition (T = 25 °C, no added cosurfactants, oil with an equivalent alkane carbon number (EACN) of zero and for ionic surfactants, a total salinity (S) of 1 g NaCl/100 mL). A recent article demonstrated the validity of the Cc concept, where Hn was assessed via oil and water solubilization radii. Here, we assess Hn from the characteristic length (ξ) obtained from the analysis of SAXS profiles of microemulsions produced at semicharacteristic conditions (characteristic condition but varying S). The predicted relationship, -L·Hn,semicharacteristic = Ccbi + bi·ln(S), was confirmed with the five ionic surfactants explored. The SAXS-assessed Cc (Cc = Ccbi/bi) values are consistent with those obtained from solubilization studies and phase inversion scans. The Cc-SAXS method provides a way to assess the hydrophobicity of ionic surfactants directly, avoiding the bias that could be introduced by cosurfactants in phase inversion studies and minimizing the impact of potential uncertainties in the surfactant volume to area ratio (vs/as) required to calculate the solubilization radii in the solubilization method.

Phase behavior of multi-stimuli-responsive ionic liquids-based micro-emulsion and its application in nano silica synthesis and curcumin encapsulation

The enrichment and separation of trace compounds, as well as the preparation of nanomaterials, are all greatly facilitated by responsive micro-emulsion. A multi stimuli-responsive ionic liquids-based micro-emulsion system was established with alkyl-N-Tropine (trans) P-methoxycinnamate ([CnTr][PMCA], TILS) as a surfactant. The factors affecting the phase behavior of micro-emulsion, such as the structure of the TILS, cosurfactant, oil, temperature, pH, and salinity, were investigated by multiple research methods. Five zones were identified inside the micro-emulsion phase region as O/W, B.C., liquid crystal (L.C.), L.C.+B.C., and W/O. The TILS-based micro-emulsion displayed high stability at measured temperatures. High salinity contributed to a higher water solubilization capacity of the micro-emulsion. For the potential applications of the micro-emulsion, with sizes ranging from 20 to 70 nm, nanoscale silica particles showed persistent stability. High levels of drug loading rate (about 8.6 mg·g−1) and encapsulation efficiency (about 99%) of the micro-emulsion loaded with curcumin were attained.

The Preparation of Electrolyte Hydrogels with the Water Solubilization of Polybenzoxazine.

Polybenzoxazine (PBZ) exhibits excellent heat resistance, and PBZ derivatives have been designed and synthesized to achieve high performance. However, the application range of PBZ is limited by the strong interactions between molecular chains and its low solubility in organic solvents, thereby limiting its processability. This study focused on the benzoxazine structure as the molecular backbone of new hydrogel materials that can be applied as electrolyte materials and prepared functional gel materials. Here, we prepared hydrogels by water-solubilizing PBZ derivatives, which typically exhibit low solubility in organic solvents. Although studies on the hydrophilization of PBZ and its complexation with hydrophilic polymers have been conducted, no studies have been performed on the hydrogelation of PBZ. First, the phenol in the organic solvent-insoluble PBZ thin film obtained after the thermal ring-opening polymerization of the monomer was transformed into sodium phenoxide by immersion in a NaOH aqueous solution to water-solubilize it and obtain a hydrogel thin film. Although the hydrogel thin film exhibited low mechanical strength, a free-standing hydrogel film with improved strength was obtained through the double network gelation method with an acrylamide monomer system. The physical properties of the polymer composite hydrogel thin film were evaluated. The ionic conductivity of the hydrogel thin films was in the order of 10-4 S cm-1, indicating the potential of PBZ as an electrolyte hydrogel material. However, improving its ionic conductivity will be undertaken in future studies.

Insights into the self-assembly of water reverse micelles and solubilization process in liquid and supercritical solvents: A molecular dynamics study

This article reports on a full-atomic classical molecular dynamics simulation investigating the self-assembly process of reverse micelles in carbon tetrachloride and supercritical carbon dioxide (scCO2). The study explores the impact of solvent type, surfactant structure (sodium dodecyl sulfate and sodium bis(3,5,5-trimethyl-1-hexyl) sulfosuccinate), water/surfactant ratio, and surfactant concentration on the self-assembly, size, and shape of reverse micelles. The results indicate that at low water/surfactant ratios in scCO2, irregularly shaped reverse micelles are formed, whereas spherical micelles form as the water/surfactant ratio increases. In scCO2, there is always a small fraction of free water molecules present, whereas in CCl4, all water molecules are surrounded by surfactants. The distribution of head groups of di-chain surfactants over the surface of micelles is observed to occur in groups of several surfactants, forming salt bridges through sodium cations. The solubilization of methyl orange by reverse micelles in pure scCO2 and scCO2 modified with ethanol has also been investigated. The study reveals that the hydrophilic part of the solute is located in the water core of the micelle, while the tail is positioned in the hydrophobic crown. Moreover, the presence of ethanol increases the number of reverse micelles compared to the system without ethanol. Ethanol molecules primarily reside on the surface of the water core, forming stable hydrogen bonds with surfactants, as well as shorter-lived hydrogen bonds with water molecules.

Geochemical characteristics and their implication of light hydrocarbons in natural gas from the Lower Paleozoic carbonate reservoirs in the Daniudi gas field, Ordos Basin

The genetic types and source of the Lower Paleozoic natural gas in the Ordos Basin in China are controversial, and the geochemical characteristics of light hydrocarbons of natural gas in the fifth member of the Ordovician Majiagou Formation (O1m5) are studied by taking the Daniudi gas field as an example, in order to reveal the gas origin and source. The C5−7 light hydrocarbons are dominated by iso-alkanes rather than the n-alkanes, and the methyl cyclohexane (MCH) content is mostly higher than the nC7 content in C7 light hydrocarbons. The K1 and K2 values range from 1.06 to 1.16 and from 0.33 to 0.81, respectively. The geochemical characteristics of light hydrocarbons indicate that the Lower Paleozoic natural gas in the Daniudi gas field is mainly coal-derived gas which has been generated by the humic source rocks in the Upper Carboniferous Taiyuan Formation (C3t), with insignificant contribution by the O1m sapropelic source rocks. The O1m5 gas pools were accumulated through downward and lateral migration in the free phase. The aromatic contents in C6-7 and nC7/MCH ratios are easily affected by the factors such as water solubilization, adsorption in migration, evaporation, and migration fractionation, therefore, secondary alteration needs to be considered when using these parameters to identify the gas origin.

Natural hydrogen and blend gas: a dynamic model of accumulation

Natural hydrogen is accumulating in the form of “blend gases”, with the association mainly of methane, nitrogen, and helium. The extreme variation of proportion between these four gas compounds is explained through a model of dynamic accumulation, where some compounds as helium are chemically inert in the reservoir, nitrogen almost inert, methane is altered into CO2, and hydrogen is severely altered mainly in protons, and in a smaller degree in methane. All the gas compounds may also leak out of the reservoir, either through advection without any chemical fractionation, or through solubilization in water and diffusion, inducing a variable composition for the residual gas remaining in the accumulation.The model can explain the concomitance of high concentration of helium, clearly accumulated through geological time, with hydrogen, supposedly renewable in human time scales. With the best choice of parameters for the model, the ages of hydrogen accumulation known in the World are small, the hydrogen being diluted more and more in nitrogen and methane through geological time. Diffusive leakage through water appears negligeable compared to advective leakage and compared to hydrogen reactivity in the reservoir. The example of the hydrogen field of Bourakébougou would present an age of 500 years according to the model. Diffusive leakage through water solubilization has a small effect on gas composition. Advective leakage, even not fractionating, has a significant effect on accumulated gas composition, because of its mixing with active deep fluxes. During future production of natural hydrogen accumulations, it is predicted that the proportion of hydrogen should increase during production time, whereas the helium and nitrogen concentrations should decrease.

The complexation of lanthanides by glycolamide extractants: Evidences from electronic spectroscopy and DFT calculations

The extraction of trivalent metal ions (Nd and Eu) in N,N,N’,N’-tetraoctyldiglycolamide (TODGA) was compared with N,N-di-octyl-2-hydroxyacetamide (DOHyA), a monoglycolamide derivative of TODGA. The studies based on UV–visible spectroscopy showed the differences in Nd(III) ion complexation for the extractants in both polar and non-polar diluents. n-Dodecane modified with 5 vol% 1-octanol was employed as diluent for extraction studies with TODGA, while DOHyA was studied with no modifiers in n-Dodecane. Time resolved luminescence spectroscopy studies of the Eu(III) extracted organic phases showed strong complexation of metal ion with both the extractants. The co-extraction of water in DOHyA solvent phase during Eu(III) extraction from nitric acid medium was found to be more as compared to TODGA. The better solubilization of water in DOHyA solvent was substantiated by DFT analysis of the optimized electronic structures of Eu-DMHyA (dimethyl hydroxyacetamide) and Eu-TMDGA (tetramethyldiglycolamide) complexes.

СОЛЮБИЛИЗАЦИЯ ВОДЫ ДЕТЕРГЕНТАМИ

The issue of solubilization of water by various detergents is considered. It has been established that with an increase in the base number of the detergent, the solubilization of water increases. The influence of the steric factor, determined by the structure of the detergent, on the efficiency of neutralization of acidic products by the latter is noted. Solubilization is proposed to be considered as an intermediate stage in the general reaction of the interaction of water with detergents, leading to destabilization of the system.

Microemulsion system formed with new piperazinium-based surface-active ionic liquid

The intensive use of ionic liquid (IL) in various researches and applications induces the imperative need of specific functional ILs. Present work introduces a novel piperazinium-based surface-active IL (SAIL) which shows great potential for the preparation of microemulsion (ME), namely 1-(2-hydroxyethyl)piperazinium laurate ([HEP][C11CO2]). Important fundamental physicochemical properties have been investigated, revealing higher surface activity for the SAIL than common surfactants. [HEP][C11CO2] is able to solubilize different oil components and water with short-chain alcohol as cosurfactant, forming stable ME in a wide range of ratio of water to oil, and a broad microemulsion region covering over 50 % of the pseudo-ternary phase diagram can be found. The structural transition of water-in-oil to bicontinuous occurs at the water content of 0.60 to 0.65 for the SAIL/1-butanol/n-heptane/water systems at the ratios of SAIL to 1-butanol of 1:1 and 1:2, as confirmed by electrical conductivity measurements. Moreover, the solubilization of oil and water was investigated in the presence of NaCl, KCl or MgCl2, and the optimal salinity was determined. While Mg2+ induces formation of emulsion probably due to the poor solubility of Mg2+/[C11CO2]− complexes, the ME system is much more stable with the presence of monovalent ions like Na+ and K+. For the NaCl-contained system, the salinity window of the bicontinuous phase changes from 0.030 to 0.025 and 0.020 when the temperature rises from 15 °C to 25 °C and 35 °C, which mainly results from the enhanced hydrophobic interactions and the weakened electrostatic interactions. Present studies elucidate that [HEP][C11CO2] is feasible to form various microemulsion systems which may have extensive potential in further applications like size-controlled syntheses and oil recovery.

Enhancement of water solubilization of quercetin by meglumine and application of the solubilization concept to a similar system

In the present work, meglumine, an aminocarbohydrate derived from glucose with a secondary amine group, was found to be able to increase the water-solubility of the well-known and beneficial flavonoid quercetin. pH proves to be a fundamental factor for this purpose. The solubility enhancement as a function of the pH as well as the mechanism behind this phenomenon were investigated. The state of charge of quercetin depending on pH has been estimated from the calculation of the pKa by COSMO-RS. A compromise between a satisfying solubility enhancement (6–7-fold) and reasonable stability against oxidation (70–75 % of quercetin still intact after 2 h) was found around pH 8. The solubilization mechanism is believed to be based on proton exchange between quercetin and the amine function of meglumine, and on the ability of meglumine to form numerous hydrogen bonds with water molecules through its five hydroxyl groups. Finally, it is shown that the underlying solubilization mechanism can be extended both to other polyphenols and to other hydrotropes.

Effect of alcohols on water solubilization in surfactant-free diesel microemulsions

Microemulsions are preferred over other methods of mixing two immiscible liquids as they are thermodynamically stable, but require a high percentage of surfactant. Surfactant-free microemulsions, on the other hand, use an amphisolvent instead of surfactants, making them an economical alternative. The current experimental investigation focuses on formulation of surfactant-free water-in-diesel microemulsions with the help of straight-chain aliphatic alcohols as diesel replacement for IC engines. Dynamic light scattering measurements revealed these microemulsions had average water droplets of less than 10 nm. The microemulsions were formulated using single-alcohol, two-alcohols, and three-alcohols. It becomes crucial to study the water solubilization of microemulsions as the addition of water in diesel improves combustion efficiency. The water solubilization in surfactant-free microemulsions was found to be dependent on the hydrophilic–lipophilic balance (HLB) of the alcohols, as it determined the solubilizing property of alcohol. The microemulsions could only be formulated in the HLB range of 6.27 to 7.76 and were found to have maximum water solubilization in the HLB range of 6.67 to 7.63. Butanol had the highest water solubilization in a single alcohol system, butanol–ethanol in the ratio of 3:1 for the two-alcohol system, and propanol–butanol–octanol in the ratio of 3:1:1 for three-alcohol systems. Alcohols with higher HLB were more hydrophilic, causing migration of alcohol from the interface to bulk water. In comparison, alcohol with a lower HLB caused the migration of alcohol from the interface to bulk diesel, which resulted in reduced water solubilizations. Hence, the microemulsions could not be formulated beyond specific HLB range. The formulated microemulsions could be a prospective substitute for diesel fuel as alcohols can be derived from renewable routes.

Ultra‐Bright Near‐Infrared Sulfonate‐Indolizine Cyanine‐ and Squaraine‐Albumin Chaperones: Record Quantum Yields and Applications

AbstractThe design of bright, high quantum yield (QY) materials in the near‐infrared (NIR) spectral region in water remains a significant challenge. A series of cyanine and squaraine dyes varying water solubilizing groups and heterocycles are studied to probe the interactions of these groups with albumin in water. Unprecedented, ′ultra‐bright′ emission in water is observed for a sulfonate indolizine squaraine dye (61.1 % QY) and a sulfonate indolizine cyanine dye (46.7 % QY) at NIR wavelengths of >700 nm and >800 nm, respectively. The dyes presented herein have a lower limit of detection than the most sensitive dyes known in the NIR region for albumin detection by at least an order of magnitude, which enables more sensitive diagnostic testing. Additionally, biotinylated human serum albumin complexed with the dyes reported herein was observed to function as an immunohistochemical reagent enabling high resolution imaging of cellular α‐tubulin at low dye concentrations.

Investigation of AOT/isooctane/water reverse microemulsion system with the presence of different mass ratios of SDS: Conductivity and water solubilization

The aim of this study is to investigate the performance of mixed surfactants reverse microemulsion systems by adding different proportions of sodium dodecyl sulfate (SDS) to sodium bis (2-ethylhexyl) sulfosuccinate(AOT) /isooctane/water reverse microemulsions. The effects of solubilization water, conductivity and KCl ionic strength on the formation boundary and formation process of AOT/isooctane/water reverse microemulsion were investigated by combining experiments with dissipative particle dynamics (DPD) simulations. The formation mechanism is initially explored by mutual validation of experiments and simulations. This approach can help researchers to quickly infer structural changes and also reduce the cost of experimental development. Based on the performance of the AOT reverse microemulsion system, AOT-SDS reverse microemulsion system was prepared with the mass ratios of AOT: SDS of 9:1, 7:3, 5:5, 3:7, 1:9. The reverse microemulsion with AOT: SDS of 9:1 demonstrated the best characteristics in water solubilization and stability. By comparing the performance of two microemulsions to demonstrate that SDS changed the structure of AOT reverse microemulsion and its solubilization water volume and stability. Moreover, we believe that the AOT-SDS/isooctane/water microemulsion system is worthy to be developed by comparing similar mixed surfactant microemulsion systems.