Solitary Glioma Research Articles

Clinical characteristics and prognostic factors of solitary and multiple adult gliomas: a retrospective study based on propensity score matching.

This study aims to compare the survival and prognostic factors in patients with solitary gliomas to those with multiple to improve the understanding of multiple gliomas and investigate their heterogeneous dissemination pathways. Data on 358 patients diagnosed with adult gliomas confirmed by postoperative pathology were retrospectively collected and analyzed. The clinical characteristics, survival rates and prognosis of patients were analyzed by propensity score matching (PSM). Between the two groups, statistically significant differences were identified in multiple general clinical characteristics, including age, pathological grade, lesion location, 1p19q co-deletion, IDH1 mutation, MGMT promoter methylation expression rate, p53 mutation and NF1 mutation (p<0.05). Before PSM, the mOS for patients with multiple gliomas was shorter than that for those with solitary (p=0.0045). Multivariate Cox regression analysis revealed that age, pathological grade IV, and absence of concurrent chemotherapy were significant risk factors affecting OS. Pathological grade IV, ki-67 expression range of 25-50%, and absence of concurrent chemotherapy were identified as risk factors for PFS. After PSM, the prognostic factors associated with OS were age and concurrent chemotherapy, while those associated with PFS were ki-67 expression range of 50-75% and lesion located in the right frontal lobe (p<0.05). The prognosis for multiple gliomas is extremely poor, which is related to the fact that the most common pathological types are glioblastomas and the surgical procedure is challenging. Concurrent chemotherapy and radiotherapy are the strongest protective prognostic factors, and the differences in their molecular pathology expression compared to solitary gliomas remain for further investigation.

Multicentric Glioma Develops via a Mutant IDH1-Independent Pathway: Immunohistochemical Study of Multicentric Glioma

Multicentric gliomas are very rare. Due to differences in their tumor types they remain enigmatic. We focused on the pathogenesis of multicentric gliomas and compared their immunoprofile with that of solitary gliomas. This retrospective study included 6 males and 8 females with multicentric glioma (8 glioblastomas, 2 anaplastic astrocytomas, 4 diffuse astrocytomas). Their age ranged from 27 to 75 years and all were treated between 2004 and June 2015. The expression of mutant isocitrate dehydrogenase 1 (IDH1), α-thalassemia X-linked intellectual disability (ATRX), p53, phosphatase and tensin homolog (PTEN), and epidermal growth factor receptor (EGFR) was examined immunohistochemically; for 1p19q analysis we used fluorescence in situ hybridization (FISH). In all patients, immunohistochemical staining was negative for mutant IDH1 and cytoplasmic PTEN; only 1 patient (7.1%) manifested nuclear PTEN positivity. FISH for 1p19q codeletion was negative in all 9 examined samples; 5 of 14 specimens (35.7%) were p53-positive, 9 (64.3%) were EGFR-positive, and 4 (28.6%) were ATRX-negative. The MIB-1 labeling index was 0.9-15.6% for grades II and III, and ranged between 17.3 and 52.4% for glioblastoma. Our results suggest that the pathogenesis of multicentric gliomas is different from the mutant IDH1-R132H pathogenesis of lower-grade glioma and secondary glioblastomas. More studies are needed to confirm the molecular mechanisms underlying the pathogenesis of multicentric glioma.

Differential expression of microRNA in paraffin-embedded tissue in patients with multiple and solitary gliomas: a preliminary study

Objectives To compare the differences of microRNA (miRNA) expression profile of multiple and solitary gliomas in formalin-fixed and paraffin embedded (FFPE) tissue and to preliminarily investigate the possible roles in glioma invasion and migration for the significantly different miRNA and its target genes. Methods FFPE specimens in patients with multiple glioma (n=6) and solitary glioma (n=6) were selected. The total RNA was extracted and detected; the gene chip technology was used to detect miRNA expression profile. The chip analysis software was used to analyze the data of the results. The miRNAs of significant expression differences of the multiple and solitary gliomas were screened. Then the biological information database was used to predict the target genes of miRNA of the most significant expression differences. Results The total RNA quality extracted from all the FFPE tissue specimens met the standard of quality control. There were significant differences in 128 different miRNAs in the multiple glioma compared with the solitary glioma (multiple >5 or 50), they were miR-21, miR-455, miR-199, miR-410, miR-148a, miR-335, miR-34b, miR-664a, miR-542, and miR-4453, respectively. Five miRNAs were significantly downregulated (multiple <0.05), they were miR-200a, miR-451, miR-29a, miR-101, and miR-96. Based on the most significant prediction results of the miR-21 and miR-200a target genes of upregulation and downregulation, their multiple target genes involved in tumor invasion and migration related biological function. Conclusions FFPE tissue may meet the subsequent experimental study, such as miRNA chip, etc. and thus provide rich resources for the research of the miRNA biological function and exploration. Multiple glioma has differential expression of miRNA compared with solitary glioma, in which miR-21and miR-200a may be closely associated with the invasion and migration of glioma. Key words: Glioma; MicroRNAs; Microchip analytical procedures; Neoplasm invasiveness; Cell migration

MULTIPLE GLIOMAS OF THE BRAIN

Gliomas of the brain are common enough but since the works of Achucarro, Ramon y Cajal and del Rio Hortega, of Spain, and of Percival Bailey, Harvey Cushing, Wilder Penfield and J. H. Globus, in this country, much histologic interest has been aroused in these tumors. Bailey and Cushing1and Bailey2have made detailed clinicopathologic studies of the tumors of the glioma group. In contrast to solitary gliomas, multiple gliomas have been observed but rarely. Because of this, the case of multiple gliomas of the brain presented is of interest. REPORT OF CASE Clinical History. —M. A., a cattle dealer, aged 44, was admitted, in 1928, to the service of Dr. George S. Amsden, Albany Hospital, as suffering from mental disease. His wife, a son and two cousins gave the information that the patient had been in an automobile accident a little over four weeks before. He was