Solid Medium Research Articles

Cultivation Strategies of Kenaf (Hibiscus cannabinus L.) as a Future Approach in Malaysian Agriculture Industry

In recent years, kenaf has gained significant global attention as a more cost-effective, adaptable, and manageable alternative to other fibre crops. India and China, with nearly 70% of the global kenaf production, have emerged as the leading producers of kenaf plants. While kenaf was traditionally valued for its paper production, it has evolved into a multipurpose crop with diverse industrial applications over the past two decades. Conventional soil-based cultivation methods for kenaf require up to six months for plant maturity. However, in vitro propagation techniques offer a promising alternative that enables faster growth and reduced labour costs. In vitro propagation can be achieved using solid and liquid media, with limited research available on the pure liquid culture method for kenaf. This review aims to introduce and compare the production of kenaf using solid and liquid media, with a specific focus on the emerging country of Malaysia, which seeks to harness the potential of kenaf cultivation for the 15th Sustainable Development Goal, “life on land”, and its contribution to the economy.

Enhancing the Cryopreservation System of Larch Embryogenic Culture by Optimizing Pre-Culture, Osmoprotectants, and Rapid Thawing

Cryopreservation is considered the safe and efficient strategy for the long-term conservation of embryogenic cultures. The objective of this study was to cryopreserve the embryogenic tissues of hybrid larch to overcome the result raised by rapid growth rates of conifer embryogenic cultures necessitating frequent sub-culturing. We systematically evaluated several parameters, including the pre-culture method (liquid or solid), osmoprotectant type (DMSO, sucrose, or PEG6000), duration of cryoprotection (1–3 h), and thawing temperature (4 °C, 25 °C, or 40 °C). After one month of cryopreservation, we assessed the regeneration efficiency and maturation ability of both cryo-preserved and non-cryopreserved tissues. Our optimized protocol involves pre-culturing embryonic tissue on the solid medium with 0.4 M sorbitol for 48 h, followed by treatment with 10% DMSO, 0.4 M sucrose, and 15% PEG6000 for 1 h on ice, and immersion in liquid nitrogen with rapid thawing at 40 °C. Notably, the use of solid media during pre-culturing was crucial to enhancing the success rate of cryopreservation. Using protocol optimization, we achieved high embryogenic tissue survival rates of over 80% without affecting the ability of somatic embryogenesis. This work provides a comprehensive set of steps for routine cryopreservation of embryogenic tissues for long-term conservation in hybrid larch, along with sample protocols for cryopreservation of larch. The results demonstrate that vitrification is a reliable method for preserving embryogenic tissues of hybrid larch with broader implications for the cryopreservation of other plant species. Further optimization and standardization of protocols across different species would ensure the preservation of genetic diversity and facilitate future research in plant biotechnology that benefits human health, food security, and environmental sustainability.

LONG TERM CLASSICAL METHODS FOR THE PRESERVATION OF XANTHAN GUM-PRODUCING - Xanthomonas arboricola pathovar pruni STRAINS

The maintenance of viable and stable Xanthomonas cells is crucial for the xanthan reliable research and industrial production. The method, storage and recovery conditions should preserve both viability and phenotypical and genotypical features. Here, the effectiveness classical methods on the long-term preservation of different Xanthomonas arboricola pathovar pruni strains was to determine. Strains were preserved by monthly sub-culturing in solid medium and lyophilization. After 12 years the viability of the strains, was assessed, as well as their productive capacity and the viscosity of the xanthan gum produced by these strains kept by lyophilization and sub-culturing. Among the lyophilized strains, only those stored at -18 °C were viable after 12 years. The productive capacity of the strains were poorly affected by lyophilization, the passage of the cultures into a solid nutrition medium being sufficient for them to return to their normal metabolism. The viscosity of the synthesized xanthan gum was method-dependent and higher for the lyophilized strains. The work and its findings are new and original because a work on this topic has never been published before. The results obtained allow the breaking of paradigms regarding the preservation of Xanthomonas.

Optimizing Different Medium Component Concentration and Temperature Stress Pretreatment for Gynogenesis Induction in Unpollinated Ovule Culture of Sugar Beet (Beta vulgaris L.)

The great economic importance of sugar beet determines the ongoing biotechnological studies conducted worldwide to improve the technology of obtaining doubled haploids (DHs) using the method of unpollinated ovule culture in vitro. To improve the induction of gynogenesis, we tested the effect of thidiazuron (TDZ), temperature bud pretreatment, different concentrations of sucrose, and culturing on liquid or solid medium. Three genotypes were tested in this study. The use of TDZ at a concentration of 0.4 mg/L in solid IMB (induction medium for Beta vulgaris) induction nutrient medium with 3 g/L phytagel, 50 g/L sucrose, 200 mg/L ampicillin and cultivation at 28◦C in the dark produced up to 16.7% induced ovules. The liquid nutrient medium of the same composition induced up to 8% ovules. Increasing TDZ concentration to 0.8 mg/L resulted in reduction or total inhibition of gynogenesis, depending on the genotype. Reducing the sucrose concentration to 20 g/L or increasing it to 80 g/L was not effective. In all three genotypes, the absence of temperature pretreatment of buds (5–6 °C) showed the best results. The plant regeneration with MS nutrient medium of 20 g/L sucrose, 3 g/L phytagel, 1 mg/L 6-benzylaminopurine (BAP) and 0.1 mg/L gibberellic acid (GA3) resulted in up to seven shoots from one induced ovule in the most responsive genotype. We showed by flow cytometry, chromosome counting and chloroplast number assessment that all regenerant plants were haploid (2n = x = 9).

Culture of Steinernema glaseri on three solid media and their virulence against Galleria mellonella larvae

Culture of Steinernema glaseri on three solid media and their virulence against Galleria mellonella larvae

Numerical Computation of Temperature Rise of Gas‐Insulated Transmission Lines Considering Thermal Properties of Insulating Gas

Accurate temperature rise prediction of the gas‐insulated transmission lines (GIL) can help to lower the cost of equipment through optimization design. To investigate the temperature rise of GIL and the heat transfer efficiency of new eco‐friendly insulating gas, the electromagnetic‐thermal‐fluid field coupling method that considers the thermal properties of solid and gaseous media is proposed. The finite element method is applied to solve the eddy‐current field and obtain the Joule loss of the conductive rod and the enclosure accurately. The GIL temperature distribution of different insulating gases is computed based on their thermophysical parameters. The heat transfer efficiency is found to be positively correlated with gas density, constant pressure heat capacity and thermal conductivity. A parameter that characterizes the heat transfer efficiency of the insulating gas is defined, such that a larger value indicates a higher heat transfer efficiency. This parameter helps to quickly determine the heat transfer capacity of new eco‐friendly insulating gas, and facilitate the optimal design of GIL equipment. © 2023 Institute of Electrical Engineer of Japan and Wiley Periodicals LLC.

Isolation and molecular characterization of potassium-solubilizing bacteria from limestone mountain of Bahorok, Langkat District, Indonesia

Abstract. Bangun IH, Hanum H, Sabrina T. 2023. Isolation and molecular characterization of potassium-solubilizing bacteria from limestone mountain of Bahorok, Langkat District, Indonesia. Biodiversitas 24: 4175-4184. In agricultural practices, ensuring an adequate supply of potassium to plants is crucial for optimal growth and productivity. However, the exchangeable K is tightly bound to soil minerals such as mica, feldspar, and clay minerals, making it unavailable for plant uptake. K-solubilizing bacteria K can dissolve potassium from the mineral layer and be available to plants. The Previous study has found 11 bacterial isolates capable of solubilizing K in Aleksandrov solid media. This study aimed to select the best K-solubilizing bacteria for solubilizing K in soil and to perform molecular identification of these bacteria. A novel finding from this study is that specific KSBs enhance the levels of exchangeable K in the soil through various mechanisms, as evidenced by increased exchangeable Ca, Mg, and soil pH. Additionally, the research identified two newly discovered bacterial species capable of potassium solubilization: Paraburkholderia phymatum and Burkholderia paludis. Furthermore, the study suggests the existence of an unknown mechanism for K solubilization, indicated by the observed increase in soil pH during the process.

Effect of sucrose preculture and culture inoculum density on cryopreservation of olive somatic embryos

This study reports improvement of a protocol for cryopreservation of olive somatic embryos by using the droplet-vitrification method on aluminum foil strips. Two different approaches were considered in order to optimize cryopreservation: investigating the effect of somatic embryos growth conditions, specifically, the influence of the inoculum density of suspension cultures from which cryopreservation explants were collected, and studying the impact of preculture with different sucrose concentrations in both solid and liquid medium. The results obtained showed a significant effect of inoculum density on cryopreservation evaluated as regrowth rate, although a significant influence on recovery percentage could not be inferred. Sucrose preculture significantly improved recovery rates after cryopreservation. The best results were achieved with somatic embryos previously incubated in liquid ECO medium supplemented with 0.2 M sucrose for 28 days. After this treatment, 90% of explants resumed embryogenesis 12 weeks after thawing. Preculture in liquid medium containing 0.2 M sucrose also had a significant influence on the initial response after cryopreservation, with first signs of regrowth resumption 3 days after thawing, compared to 24-25 days in control, non-precultured embryos. Surviving explants continued actively growing, exhibiting the growth pattern normally observed in olive. As revealed by the histological analysis, cell proliferation greatly increased in somatic embryos precultured on sucrose-enriched media. Cellular features of meristematic and proembryogenic cells, mostly constituting the proliferative regions, make them more prone to survive cryopreservation, thus explaining higher recovery rates found after these treatments.

Secretome analysis revealed that cell wall remodeling and starch catabolism underlie the early stages of somatic embryogenesis in Pinus nigra.

Somatic embryogenesis is an efficient mean for rapid micropropagation and preservation of the germplasm of valuable coniferous trees. Little is known about how the composition of secretome tracks down the level of embryogenic capacity. Unlike embryogenic tissue on solid medium, suspension cell cultures enable the study of extracellular proteins secreted into a liquid cultivation medium, avoiding contamination from destructured cells. Here, we present proteomic data of the secretome of Pinus nigra cell lines with contrasting embryogenic capacity, accounting for variability between genotypes. Our results showed that cell wall-related and carbohydrate-acting proteins were the most differentially accumulated. Peroxidases, extensin, α-amylase, plant basic secretory family protein (BSP), and basic secretory protease (S) were more abundant in the medium from the lines with high embryogenic capacity. In contrast, the medium from the low embryogenic capacity cell lines contained a higher amount of polygalacturonases, hothead protein, and expansin, which are generally associated with cell wall loosening or softening. These results corroborated the microscopic findings in cell lines with low embryogenic capacity-long suspensor cells without proper assembly. Furthermore, proteomic data were subsequently validated by peroxidase and α-amylase activity assays, and hence, we conclude that both tested enzyme activities can be considered potential markers of high embryogenic capacity.

2D and 3D modelling of Molucca Sea double subduction zone based on primary waves travel time seismic tomography inversion

Molucca Sea is one of the areas with a high level of seismicity in Indonesia due to its location between three plates. The activities of them forming a quite unique subduction zone. The Eurasian Plate (Sangihe Microplate) move to the East and The Philippine Sea Plate (Halmahera Microplate) continues to Westward caused The Molucca Sea Microplate to be pressed, and subducts in two opposite directions modelat the same time forming an inverted “U”. This research used 3663 hypocenters and 9 BMKG stations in the last 5 years (2016 - 2021) with a magnitude of 3 - 8 Mw and a depth of 3 - 630 kilometers. Seismic inversion of travel time tomography is used to produce 2D models of the subduction zone around The Molucca Sea. The distribution of high ΔVp values is ranging from 3.5% to 4% is associated with a solid medium, like The Sangihe Plate, Halmahera Plate, and The Molucca Sea Plate subduction slab. While the low ΔVp is ranging from 1.8% to 2% is associated with the destruction zone and the presence of thermal fluids such as magma or partial melting (the presence of volcanoes). The 3D model is based on the Gaussian Process Regression principle using magnitude and depth data as initial model parameters. Plotting of the thrust faults at the front of the two arcs, namely The West Sangihe Thrust Fault and The East Halmahera Thrust Fault. It can be seen that the subduction under The Sangihe Microplate is deeper (627.2 kilometers) has the average subduction angle of 45.3° compared to the subduction under The Halmahera Microplate (280.0 kilometers) has the average subduction angle of 35.8°

Improved ultraviolet -visible optically transmittance and luminescent properties in Yb, Lu: Sr5(PO4)3F transparent ceramics via Lu3+ co-doping

The asymmetric hexagonal Sr5(PO4)3F (S-FAP) crystal material is considered to be the most suitable solid state laser gain medium for small laser diode pumping in the future due to its large absorption, emission interface, and long fluorescence lifetime. However, the mediocre optical transmittance of S-FAP transparent ceramics and the degradation of luminescence properties due to the doping of Yb activated ions seriously hinder its application prospects. In view of this, a series of 0.02Yb, xLu: S-FAP (x = 0–0.02) transparent ceramics with excellent optical properties were synthesized by hot pressing sintering. The powder SEM results show that Lu doping has no obvious effect on the morphology, grain size, and dispersion of powder. The linear transmissivity curves show that the ultraviolet (200 nm) and visible (500 nm) transmissivity increase by 54 % and 17 %, respectively, with Lu doping compared with the undoped ceramic samples. The surface SEM of ceramics revealed that Lu3+ promoted the increase of ceramic grain size significantly. The emission spectrum and fluorescence decay curves at room temperature also show that the emission intensity and fluorescence lifetime of ceramic samples increase significantly with Lu co-doping.

Assessment of Processing Parameters of Pack Silicon Cementation onto P265GH Grade Steel.

The quantification of the single or combined variation of the process parameters specific to a thermochemical treatment is the key to a full factorial experiment and a first step in the development of computer-aided process engineering. Powdery solid media are frequently used in the practice of thermochemical treatments when the number of processed products is reduced, additional investments are not justified, or when there are no technological alternatives. The control of the process carried out in such powdery solid media involves both the control of the thermal and temporal parameters of the process on the layer growth kinetics and its phase composition, as well as the ratio of the powdery solid mixture mass percentage (active and neutral components, reaction activators, and components with the role of blocking the sintering tendency of the medium particles). In this paper, using the specific full factorial experiment (that is, a first-order complete factorial experiment (CFE)), the full model of the regression equation of the interactions between the specific process parameters of the silicide layer formation in a powdery solid media, applied to the low alloy P265GH steel grade and used in the petrochemical industry, were evaluated. Fe-ARMCO was chosen as the reference in the experimental research carried out.

Annotated dataset for deep-learning-based bacterial colony detection

Quantifying bacteria per unit mass or volume is a common task in various fields of microbiology (e.g., infectiology and food hygiene). Most bacteria can be grown on culture media. The unicellular bacteria reproduce by dividing into two cells, which increases the number of bacteria in the population. Methodologically, this can be followed by culture procedures, which mostly involve determining the number of bacterial colonies on the solid culture media that are visible to the naked eye. However, it is a time-consuming and laborious professional activity. Addressing the automation of colony counting by convolutional neural networks in our work, we have cultured 24 bacteria species of veterinary importance with different concentrations on solid media. A total of 56,865 colonies were annotated manually by bounding boxes on the 369 digital images of bacterial cultures. The published dataset will help developments that use artificial intelligence to automate the counting of bacterial colonies.

Double phase culture system mediated enhanced protocol for shoot proliferation of Vanilla andamanica Rolfe. - an endemic wild relative of commercial Vanilla from the Andaman and Nicobar Islands

A liquid overlay culture system in micropropagation improved shoot proliferation in many species. In the present investigation, we have developed an enhanced shoot proliferation protocol using the double phase culture system (DPS) for Vanilla andamanica, an endemic species of the Andaman and Nicobar Islands recognized under the vulnerable category by the IUCN. In vitro generated nodal explants were used for shoot proliferation experiments and were tested in Murashige and Skoog medium augmented with various cytokinins (BAP, KIN, 2iP) and auxins (IBA, NAA, IAA) to produce a maximum of 3.24 ± 0.08 shoots per explant in 5 µM BAP and 5.23±0.67 roots per shoot in basal MS medium. A remarkable increase in shoot regeneration was observed when the nodal explant was cultured on DPS system with optimum cytokinin (5 µM BAP). On evaluation of the influence of DPS and conventional single-phase systems (SPS), (solid medium) exhibits an improved multiplication rate on a DPS with optimal BAP (5µM) with an average of 8.66 ± 0.17 shoots per explant, which represents a 2-fold increase over the rate of SPS + 5 µM BAP. The plantlets were rooted at 100% frequency in half-strength MS medium devoid of auxins and acclimatized with 100% success. A double-phase mediated enhanced shoot proliferating procedure could be employed for large scale multiplication and commercial breeding trials for better results with regard to V. andamanica.

Identification and characterisation of antimicrobial compound produced by probiotic Alkalihalobacillus clausii 088AE.

In the present study, Alkalihalobacillus clausii 088AE was evaluated for the production of antimicrobial compounds, their characterisation, and identification. The results showed that, 48-h-old A. clausii supernatant is able to inhibit the growth of indicator bacteria Micrococcus luteus MTCC 106T (7.0 ± 0.51 mm). The cultivation of 088AE on solid media along with XAD16N beads for 5 days, and isopropanol extraction of antimicrobial compound thereof showed enhanced antimicrobial activity (19.67 ± 0.58 mm) against indicator strain. Further purification with a reversed-phase C18 cartridge yielded a powder with 389.12 ± 10.08 μg of protein per mg. The UV spectra of the sample revealed the characteristic aromatic amino acid peaks at 262 and 276 nm. The results of triple-quadrupole mass spectrometry showed a peak of 1055 m/z, confirming the existence of 10542+, i.e., 2108 Da, suggestive of class I lantibiotic clausin. Furthermore, in silico analysis confirmed the presence of genes responsible for the production of lanthipeptide clausin. The clausin is stable in the presence of proteases (trypsin and pepsin), high temperature (100 °C), pH up to 11 and showed antimicrobial activity against Listeria monocytogenes ATCC 19115, M. luteus, and Staphylococcus aureus ATCC 6538P. Therefore, it could be useful to control gut infections caused by Gram-positive pathogens.

Computational and spectral analysis of derivatives of 9,9-dimethyl-9,10-dihydroacridine and 10-Phenyl-10H-phenothiazine-5,5-dioxide with hybridized local and charge-transfer excited states for optoelectronic applications

Aiming to combine the advantages of both prompt fluorescence and thermally activated delayed fluorescence in single emitter, molecular design of emitters with hybridized locally excited and charge transfer states were investigated by computational approaches and optical spectroscopy. Taking into account the results of the theoretical screening, the most promising derivatives of 9,9-dimethyl-9,10-dihydroacridine and 10-phenyl-10H-phenothiazine-5,5-dioxide based with the different linking topology (meta- and para-isomers) were selected for the synthesis and experimental investigations. Both the compounds exhibit ultraviolet LE emission peaking at ca. 360 nm, green ICT peaking at ca. 510 nm, and deep-blue HLCT emission peaking at ca. 430 nm when they are molecularly dispersed in the solid media of the different polarity. The developed emitters allow to obtain deep-blue electroluminescence for the host-containing OLEDs and green electroluminescence of host-free devices with the efficiency of exciton production of 42 and 73%, respectively. Efficient exciton production is due to the spin-flip switching via thermally activated processes which is much more efficient than prompt fluorescence. Showing the impact of the linking topology, the para-isomer demonstrated more efficient triplet harvesting in OLEDs than meta-isomer. A detailed discussion on the structure–property relationships and on some discrepancies between the results of the results of theoretical calculations and spectral analysis allows to obtain important insights on the photophysical properties of these compounds.

Streptomyces marispadix sp. nov., isolated from marine beach sediment.

A novel actinomycetal strain, designated M600PL45_2T, was isolated from marine sediments obtained from Ingleses beach, Porto, on the Northern Coast of Portugal and was subjected to a polyphasic taxonomic characterisation study. The here described Gram-reaction-positive strain is characterised by the production of a brown pigment in both solid and liquid medium and forms typical helical hyphae that differentiate into smooth spores. The results of a phylogenetic analysis based on the 16S rRNA gene sequence indicated that M600PL45_2T has a high similarity to two members of the genus Streptomyces, Streptomyces bathyalis ASO4wetT (98.51 %) and Streptomyces daqingensis NEAU ZJC8T (98.44 %). The genome of M600PL45_2T has a size of 6 695 159 bp, a DNA G+C content of 70.71 mol% and 5538 coding sequences. M600PL45_2T grows at 15-37 °C and with a maximal growth rate between 25 °C and 30 °C. Growth at pH 6.0 to 9.0 with the optimal range between 6.0 and 7.5 was observed. M600PL45_2T showed a high salinity tolerance, growing with 0-10 % (w/v) NaCl, with best growth with 1-3% (w/v) NaCl. Major cellular fatty acids are iso-C15:0 (25.03 %), anteiso-C15:0 (17.70) and iso-C16:0 (26.90 %). The novel isolate was able to grow in media containing a variety of nitrogen and carbon sources. An antimicrobial activity screening indicated that an extract of M600PL45_2T has inhibitory activity against Staphylococcus aureus. On the basis of the polyphasic data, M600PL45_2T (= CECT 30365T = DSM 114036T) is introduced as the type strain of a novel species, that we named Streptomyces marispadix sp. nov.

Paracholeplasma manati sp. nov. isolated from Florida manatees (Trichechus manatus latirostris).

Four novel strains of a member of the genus Paracholeplasma (OakleyT, Holly, Lorelei and Ariel) were isolated from skin of Florida manatees (Trichechus manatus latirostris). These strains were phenotypically and genetically characterized and compared with the known species of the genera Acholeplasma (A.), Alteracholeplasma (Al.), Haploplasma (H.), Paracholeplasma (P.) and Mariniplasma (M.). All the strains produced acid from glucose but did not hydrolyze arginine or urea. All were propagated in ambient air supplemented with 5±1 % CO2 at 35-37 °C using SP4-Z, Columbia and brain-heart infusion medium. Colonies on solid medium showed a typical fried-egg appearance and transmission electron microscopy revealed a typical mycoplasma-like cellular morphology. The results of phylogenetic analyses based on partial 16S rRNA, rpoB, gyrB and parE gene sequences and the whole proteome data indicated that the novel species is a unique species but phylogenetically closely related to Paracholeplasma vituli, Paracholeplasma morum and 'Paracholeplasma brassicae'. The average nucleotide identity and digital DNA-DNA hybridization values between strain OakleyT and the closely related species were significantly lower than the accepted thresholds for describing novel prokaryotic species at the genomic level. On the basis of the genomic, phenotypic and phylogenetic properties, the novel strains represent a novel species of the genus Paracholeplasma, for which the name Paracholeplasma manati sp. nov. with the type strain OakleyT (=NCTC 14352T =DSM 110686T) is proposed. The genomic DNA G+C content and complete draft genome size for the type strain are 38.35 % and 1 873 856 bp, respectively.

Neutrinos above the Earth's Surface

In article “Doppler Effect and neutrino acoustic signatures”, published in the journal “LA MULTIAPP” on February 25, 2022, I was forced to touch on an astrophysical topic when, in the data stream obtained by monitoring the acoustic noise of the earth’s crust with high amplitude resolution (more 240 dB) and in a wide frequency band (from 0.1 Hz to 50 kHz) events began to appear, the forms and frequencies of which are not typical for geophysics and seismology. Similar forms can be seen in works on the detection of acoustic traces of neutrino decay in water and ice. The possibilities of increasing the sensitivity in measurements in a solid medium are discussed. In addition, underwater and underground facilities are being built that use the effect of Cherenkov radiation. All of these methods require complex and very expensive installations on a huge scale. Even acoustic measurements in water and in wells are very laborious and expensive, and most importantly: such measurements are forever tied to a specific place. Therefore, the creation of a light, compact and mobile device for recording acoustic traces of neutrino decay is an urgent task, the solution of which will allow recording traces of neutrino decay not only in water and the earth's crust, but also in air, into space, on planets and satellites.

Combined System for the Simultaneous Delivery of Levofloxacin and Rifampicin: Structural and Functional Properties and Antibacterial Activity.

The therapy of resistant forms of tuberculosis requires the simultaneous use of several drugs, in particular, a combination of rifampicin and levofloxacin. In this paper, we aimed to design a combined system for the simultaneous delivery of these drugs for potential inhalation administration. A feature of this system is the incorporation of rifampicin into optimized liposomal vesicles capable of forming a multipoint non-covalent complex with chitosan-β-cyclodextrin conjugates. Levofloxacin is incorporated into cyclodextrin tori by forming a host-guest complex. Here, a comprehensive study of the physicochemical properties of the obtained systems was carried out and special attention was paid to the kinetics of cargo release for individual drugs and in the combined system. The release of levofloxacin in combined system is slow and is described by the Higuchi model in all cases. The release of rifampicin from liposomes during the formation of complexes with polymeric conjugates is characterized by the change of the Higuchi model to the Korsmeyer-Peppas model with the main type of diffusion against Fick's law. Microbiological studies in solid and liquid growth media a consistently high antibacterial activity of the obtained systems was shown against B. subtilis and E. coli.