The article presents the results of research on the assessment of ecosystem services of green tree stands on the territory of the first academic campus of the National University of Kyiv-Mohyla Academy using the MyTree tool. It is a part of the i-Tree tool set developed by the Forest Service of the United States Department of Agriculture (USDA) of the comprehensive program, designed to assess the ecosystem services provided by individual trees or small groups of trees. Research has established that the most important regulatory services were carbon dioxide absorption, microclimate regulation, energy saving, air purification from ozone and sulfur dioxide. Calculations by the MyTree tool show the annual cost of tree planting services on campus and its projected cumulative growth after 20 years. According to the calculations of the MyTree tool, this year’s cost of tree plantation services of the first academic campus of the National University of Kyiv-Mohyla Academy was $847.29, and according to forecasts, after 20 years it will cumulatively increase to $18,895.36. Estimates of the cost of individual components of regulatory ecosystem services are provided. The cumulative estimate over the next 20 years for CO2 sequestration was $4,129.15, which includes the sequestration of 21,963.55 kg of carbon. Air purification for 20 years is estimated at $1,342.66, which includes absorption and neutralization of 656.24 kg of O3, 117.47 kg of SO2, 54.45 kg of NO2, and 34.26 kg of solid dust particles PM2.5. Energy savings are estimated at $10,791.14, which includes energy savings of 100,448.21 kW. The tree species Aesculus hippocastanum (Bitter chestnut), Picea abies (European spruce), Picea pungens (Blue spruce), Betula pendula (Hanging birch) and Ulmus laevis (Smooth elm) provide the highest value of services. The results of this study can be used in the future to improve the planning and management of green areas of the university campus, taking into account the value of their ecosystem services.In general, the study confirmed the importance of monitoring and evaluating the ecosystem services of green spaces in urban ecosystems for making sustainable decisions regarding the development of urban areas, supporting the well-being and health of the population.