Trace inspection is a key technology for collecting crime scenes in the criminal investigation department. A lot of information can be obtained by restoring and analyzing the remaining traces on the scene. However, with the development of digital technology, digital trace inspection has become more and more popular. So, the main research of this article is the design and realization of the trace inspection system based on hyperspectral imaging technology. This article proposes nondestructive testing technology in hyperspectral imaging technology. Combining basic principles of spectroscopy and the image of residual traces such as car tires, shoe soles, and blood stains, it can identify the key traces. Then, based on the image denoising and least squares support vector machine method, this study improves the accuracy and restoration of the image. Therefore, this study designs a test for the trace inspection system for testing hyperspectral imaging technology. The test items include the performance of the trace inspection system, the noise reduction of the trace inspection system, and the ability of the trace inspection system to inspect blood stains. The final collected data are improved to get the trace inspection system based on hyperspectral imaging technology proposed in this study. Compared with the traditional trace inspection system, the experimental results show that the trace inspection system based on hyperspectral imaging technology can improve the accuracy by 5%–28%, compared with the traditional trace inspection system. The image restoration degree of the hyperspectral imaging technology trace inspection system can be improved by 1%–19%, compared with the traditional trace inspection system.