Soldier Fly Research Articles

Utilization of Black Soldier Fly and Housefly as Biodegradation Agents of Dairy Wastes and source of Animal Feed

Accumulation of dairy wastes and shortage of nutritious feed remain the major challenges in dairy cattle production. Such wastes may sustainably be biodegraded by black soldier fly (BSF) larvae and housefly (HF) larvae into their which ca be used as animal feed. This study was done to compare the capacity of using black soldier fly larvae (BSFL) and housefly larvae (HFL) to manage dairy wastes and use of such larvae as livestock feed. Biomass reduction rate, biomass reduction index, bioconversion rate, feed conversion ratio and nutritional profile of resultant BSF and HF prepupae raised on cow dung (CD) and dairy shed effluent (DSE) were determined. In order to ascertain whether the substrates had an effect on BSFL and HFL, analysis of variance (ANOVA) was used, and multiple mean comparisons at P≤0.05 were done using the Tukey HSD post-hoc test. Housefly larvae reared on DSE had the highest biomass reduction rate and biomass reduction index, even though HFL had a shorter development time than BSFL (P≤0.05). Housefly prepupae reared on DSE had the highest crude protein content of 60.1 % of all the HF prepupae counterparts and BSF prepupae reared on DSE and CD (P≤0.05), Hence HF prepupae could be an alternative source of animal feed. Depending on the needs and purpose, utilization of fly larvae to manage dairy waste is viable and effective option

Treatment of biowaste commingled with biodegradable bioplastic films using Black Soldier Fly larvae: Generation and fate of micro-plastics

The use of Black Soldier Fly (BSF) larvae is emerging as a promising alternative for biowaste (i.e. food waste) treatment, generating larval biomass and process residues, suitable for use as animal feed and fertilizer, respectively. In line with an increasing use of starch-based bioplastics in food packaging, the presence of these biopolymers and associated biodegradable microplastics (BMPs) in food waste is expected to rise. Knowledge of the generation of BMPs and their fate in the BSF treatment process is scarce, or indeed, completely lacking in the case of small-sized BMPs (<50 μm). The present study aims to investigate the generation and potential accumulation of BMPs in BSF larvae process. Food waste mixed with starch-based bioplastic films was fed to larvae and BMPs of two particle sizes (inferior to and exceeding 10 μm in diameter) were monitored over time in rearing substrate and larval biomass. BMPs concentrations in substrate were compared with larvae-free control tests. The presence of larvae favoured the generation of BMPs. Concentrations of smaller-sized BMPs (<10 μm) increased by approximately 172% in the final substrate, and accumulated in the larval biomass with a peak exceeding the initial larval concentration by over 1000% just before prepupation, which is the typical stage they are collected when used as animal feed. These results indicate a potential risk of soil contamination by BMPs when final substrate is used as fertilizer and a risk of biomagnification phenomena when larvae are used as animal feed.

Influence of different levels of black soldier fly larvae meal on growth performance and carcass quality of broiler chickens

A study was conducted to examine the impact of two inclusion levels of Black soldier fly larvae meal (BSFLM) replacing soybean meal on growth performance, nutrient utilization, carcass characteristics and meat quality of broilers. Three experimental diets based on corn-soybean meal were developed to contain 0 (control), 6 (BSF 6) and 12% (BSF 12) BSFLMfor both starter and grower phases. Each experimental diet was randomly allotted to six replicate pens (eight birds per pen). The birds were offered starter pellets from 0 to 14 day post-hatch and grower pellets from 15 to 28 day post-hatch. The experimental diets were tested for pellet durability index (PDI). There was an interaction between diet and growth phase (P < 0.001) for pellet durability index where starter diets had always a higher PDI than the grower diets, but the difference was greater for control diet than BSF 6 and BSF 12 diets. Apparent metabolizable energy (AME) of diets and coefficients of apparent ileal digestibility (CAID) of nutrients were measured on day 28 using titanium dioxide marker ratios in the diet and excreta/ileal digesta. On day 28, the weights of live body, carcass, fat pad, breast and gizzard were recorded, and then breast meat quality (meat pH, drip loss and cooking loss) was examined. Inclusion of BSFLM of up to 12 % did not reduce live weight gain or feed intake. Live weight and carcass weight were heavier in broilers fed 12 % BSFLM than controls (P < 0.02), but were not different than those fed 6 %, while controls were not different than those fed 6 %. Breast weight (percentage live weight) was lower in birds offered 12 % BSFL than in others (P < 0.04). No differences were observed between diets for the percentage weight as carcass, fat, pad and gizzard. The AME and AMEc of diets were the highest in broilers fed 6 % BSFLM diet (P < 0.005), but there were no differences between controls and those fed 12 % BSFLM. The CAID of DM, ash and N in birds fed 6 % BSFLM were greater than (P < 0.03) birds fed 12 %, but were not different than controls, which were also not different than those fed 12 %. Broiler breast meat quality was unaffected by dietary treatments. In conclusion, BSFLM at 12 % can be used effectively as a SBM replacement in starter and grower diets, without affecting the growth performance, nutrient utilization, carcass characteristics and meat quality of broiler chickens.

Improving egg quality in laying hens using calcium salt of black soldier fly larvae oil

Abstract Black soldier fly larvae (BSFL) oil has gained attention as a potential substitute for vegetable oil in feed formulations designed to improve productivity and product quality. This study aimed to investigate the impact of supplementation BSFL oil calcium salt (BSFLO-SCa) on the egg quality and gene expression involved in fat metabolism in laying hens. A total of sixty 40-wk-old ISA Brown laying hens were divided into three distinct groups: a control group (T0) that received a basic diet, and two treatment groups that were supplemented with 1% (T1) and 2% (T2) BSFLO-SCa. Each group consisted of 5 replicates, with 4 laying hens per replicate. The study indicated that the inclusion of 2% of BSFLO-SCa significantly increased egg albumen weight, height, ratio, eggshell weight, and thickness (). The inclusion of 1 and 2% of BSFLO-SCa increased the yolk lightness (L*) and yellowness (b*), but redness (a*) was decreased (). Moreover, BSFLO-SCa supplementation with 1 and 2% reduced () egg fat and cholesterol. Analysis of fatty acid profiles showed significantly increased medium-chain fatty acid (MCFA) and polyunsaturated fatty acid (PUFA) (), while monosaturated fatty acid (MUFA) reduced () with 2% BSFLO-SCa. Gene expression related to lipid metabolism, such as fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC), as well as cholesterol synthesis gene expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) were downregulated (). In conclusion, the inclusion of 2% BSFLO-SCa in the diet improved quality and reduced fat and cholesterol content in eggs from laying hens. This improvement was accompanied by downregulation of key genes involved in lipid metabolism.

Optimizing the Marketing Potential of BSF Maggot as a Decomposer of Organic Waste to Overcome Social Problems in Murtigading Village, Bantul, D.I. Yogyakarta

The improper management of organic waste has become a serious issue in Murtigading Village, Bantul, Yogyakarta. Black soldier fly (BSF) maggot cultivation offers an efficient solution for decomposing organic waste. However, the marketing of BSF maggots remains a challenge due to a lack of community knowledge and business strategies. This research aims to optimize the marketing potential of BSF maggots to address both waste management and social problems in the village. A descriptive qualitative method was employed, involving interviews, field surveys, and literature reviews. The study analyzed the strengths, weaknesses, opportunities, and threats (SWOT) of BSF maggot marketing, supported by a Pentahelix model that involves collaboration between the community, government, private sector, media, and academics. The results show that there is significant market potential for BSF maggots, with demand from industries in Surabaya requiring up to 5 tons of maggots per shipment. Furthermore, marketing through digital platforms like online shops offers a cost-effective solution for increasing sales, reducing the reliance on traditional methods. This research concludes that with improved marketing strategies and support from key stakeholders, BSF maggot cultivation could become a profitable industry while solving the village's waste problem.

Gone but not forgotten: dynamics of sperm storage and potential ejaculate digestion in the black soldier fly Hermetia illucens.

Understanding the dynamics of sperm storage is essential to unravel the complexity of post-copulatory sexual selection processes in internally fertilized species. This physiological process goes from sperm transfer during copulation to its use for fertilization. In this context, the spatiotemporal dynamics of sperm storage were described in the black soldier fly (BSF) with fluorescence and transmission electron microscopy (TEM). BSF females have compartmentalized spermathecae with a transfer compartment, the fishnet canals, and a storage compartment, the reservoirs. Spermatozoa were counted both during and after mating in the two compartments. In addition to seminal fluids, the male transfers a mass of sperm in the fishnet canals, then only 49% of the transferred spermatozoa reach the reservoirs over two days. TEM observations of the fishnet canals revealed potential digestive functions, explaining the decline in the number and viability of spermatozoa in this compartment but not in the reservoirs. After one mating, females laid up to three fertile clutches, showing no constraints on sperm quantity or quality. Spermatic and ultrastructural investigations strongly suggest that BSF ejaculate acts both as a sperm plug and as a nuptial gift, reinforcing the interest in studying this farming insect as a new model for sexual selection.

Transcriptomic response of Hermetia illucens L. (Diptera: Stratiomyidae) to wounding and Gram-negative bacterial infection

Abstract The larvae of the black soldier fly (BSFL), Hermetia illucens L. (Diptera: Stratiomyidae), are of economic interest due to their use as livestock feed component. Unraveling their response to a bacterial infection will allow us to gain a better insight into their biology. In the current study, we used RNA-Seq analysis to unravel the transcriptomic response of BSFL to wounding and infection by a Gram-negative bacterium, Pseudomonas protegens Pf-5. Five-day-old BSFL were subjected to three treatments, i.e. untreated, PBS-injection, and bacteria-injection (5000 CFU per larva) and samples were collected at three time points (2 h, 6 h, and 13 h) post- treatment. Wounding induced expression of genes encoding recognition molecules and signaling pathway genes such as PGRP-SA, and Relish, and antimicrobial peptides (AMPs) such cecropin, defensin, and attacin. At 2 h, wounding resulted in a significant upregulation of immunity-related genes whereas genes encoding for resilin and cuticle proteins were significantly downregulated. At 6 h, the expression of immunity-related genes reduced in response to wounding whereas their expression increased in infected larvae. At 13 h, the expression of immunity-related genes reduced drastically in response to wounding, while their expression increased significantly in infected larvae. Conversely, the expression of metabolism-related genes, such as trypsin and chymotrypsin, was significantly upregulated in wounded larvae at 13 h, while their expression was significantly reduced in infected larvae. Increased investments in immunity-related processes in infected BSF larvae correlated with the downregulation of genes associated with metabolic processes indicative of a trade-off. Various immunity-related genes, including those encoding cecropin, defensin, attacin, and PGRP-SA, were consistently induced only during pathogen infection, indicating their role in immunity against Gram-negative bacteria. In this study, we report multiple genes that are significantly upregulated post-bacterial infection in BSFL that may be utilized as biomarkers to monitor insect health in mass production facilities.

Replacing sea fish with black soldier fly fermentation homogenate in diet for Pelodiscus sinensis: growth, intestinal development and hepatic health

Abstract Black soldier fly larvae (BSFL) meal has the potential to partially replace fish meal in the diet of various aquatic animals. The fermentation treatment of BSFL can retain more nutrients and reduce processing and transportation costs. This study investigated the effects of partially replacing fish meal with Black Soldier Fly Fermentation Homogenate (BSFFH) on the growth performance and health of Chinese soft-shelled (Pelodiscus sinensis) turtle. The experiment was divided into 4 different groups of juvenile P. sinensis diets. The control diet included 60% compound feed and 40% sea fish. The 34%, 61%, and 82% substitution groups were fed mixtures, and varying percentages of sea fish were replaced with BSFFH. The results indicate that replacing 34% and 61% sea fish with BSFFH significantly improved growth (P &lt; 0.05). The 61% substitution significantly decreased the muscle fiber size while the 82% substitution significantly upregulated the expression of myogenic genes MYF5 and MRF4 (P &lt; 0.05). Furthermore, replacing sea fish with BSFFH significantly increased hepatic TG content (P &lt; 0.05) and induced mild liver fibrosis, while it reduced the TG content in skeletal (P &lt; 0.05). However, BSFFH did not affect subcutaneous fat weight (P &gt; 0.05). Lastly, the 34% substitution significantly increased the intestine villus height and the ratio of villus height/crypt depth, while higher substitution reduced both values. In addition, the 61% substitution increased the mucus secreted by the intestine goblet cells. In conclusion, partially replacing sea fish with BSFFH improves the growth performance and intestine structure of Chinese soft-shelled turtles. However, a high level of BSFFH could negatively affect growth performance, intestinal structure, and hepatic health.

Nutritional Profile and Antibacterial Activity of the Black Soldier Fly (Hermetia illucens) Larvae Meal as Potential Protein Source for Aquafeeds

Nutritional Profile and Antibacterial Activity of the Black Soldier Fly (Hermetia illucens) Larvae Meal as Potential Protein Source for Aquafeeds

Nutrition composition of black soldier fly (Hermetia illucens L.) prepupae reared on different organic substrates

Nutrition composition of black soldier fly (Hermetia illucens L.) prepupae reared on different organic substrates

Nutrient profile, protease and cellulase activities of protein extracted from black soldier fly (Hermetia illucens) larvae reared on various substrates

The Black Soldier Fly (BSF; Hermetia illucens) larvae are recognized for their ability to convert diverse organic materials into protein-rich biomass, depending on the substrate they consume. The composition of these substrates can significantly impact the nutrient profile and enzyme activities of the resulting maggot protein extract (MPE). Therefore, this exploratory research aimed to assess the nutrient content, protease, and cellulase activity of MPE obtained from BSF maggots reared on different substrates, with a specific focus on substrates A (comprising restaurant waste and rejected milk), B (layer manure), and C (kitchen waste). The results showed that maggot meal from layer manure had the highest protein content (45.36%) and the lowest fat content (18.44%). Amino acids in maggot meal contained high levels of glutamic acid, aspartic acid, alanine, valine, leucine, and isoleucine. Lauric acids were found in maggot meal from kitchen waste (33.79%), layer manure (32.18%), and restaurant waste and rejected milk (22.94%). Maggot meal from layer manure had the highest oleic acid content (15.13%). The protein concentration of MPE from various substrates ranged from 0.56 to 0.601 mg/ml (at 60% w/v ammonium sulfate saturation) and 0.555 to 0.609 mg/ml (at 70% ammonium sulfate saturation). The protease activity of MPE from layer manure substrates exhibited optimum activity and stability in neutral to alkaline pH, with activity levels of 0.748 U/mg at pH 7.0 and pH 11.0 (at 60% w/v ammonium sulfate saturation) and 0.774 units/mg at 70% w/v ammonium sulfate saturation. The highest cellulase activity was found in MPE from kitchen waste, which remained stable at pH 5.0-11.0. In general, maggots from different substrate sources exhibited distinct nutrient profiles and enzyme activities. Protein extract from maggots grown in layer manure showed the most suitable nutrient profile for use as an alternative source of protein feed and protease enzymes.

Structural characterization, HepG2 cell cytoprotective ability, and antioxidant mechanism of novel antioxidant peptides identified from black soldier fly larvae (Hermetia illucens L.)

This study isolated a novel antioxidant peptide from black soldier fly larvae (BSFL) using enzymatic hydrolysis. Firstly, the BSFL enzymatic hydrolysate was fractionated through ultrafiltration, with the <3 kDa fraction exhibiting the strongest DPPH and ABTS radical scavenging activity. Subsequently, this fraction was further fractionated through gel filtration chromatography and RP-HPLC. Totally, 153 peptides were identified through LC-MS/MS analysis, from which a novel peptide EDEGTYKCVLS (Pep6) was screened according to activity prediction and verification. Pep6 exhibited high radical scavenging capacity and cytoprotective effect on HepG2 cells against H2O2 damage, meanwhile significantly increasing the intracellular antioxidant enzymes activity. Molecular docking analysis indicated that Pep6 competitively bound to Keap1, thereby inhibiting the formation of Keap1-Nrf2 complex, ultimately protecting cells from oxidative stress damage. In this study, a novel antioxidant peptide Pep6 was identified from BSFL, and its antioxidant mechanism was elucidated, providing a theoretical basis for its use as a natural antioxidant.

Effects of black soldier fly larvae on biotransformation and residues of spent mushroom substrate and wet distiller’s grains

Black soldier fly larvae (BSFL) could convert a variety of organic wastes, including spent mushroom substrate (SMS) and wet distiller’s grains (WDG). Nevertheless, little is known about the conversion of these wastes by BSFL. Thus, this study investigates the conversion of SMS and WDG in five different proportions by BSFL. This study demonstrates that BSFL can convert SMS, WDG, and their mixtures. It can also encourage the humification of the substrate, increasing the amount of element in the residues. It is evident that there were differences in the carbon and nitrogen element fractionation mode as well as the microbial community present in the residue. The microbial community of the substrate and the physiochemical parameters are intimately related to this. Although the mixture treated with BSFL helps to generate a residue with more humus, it might not be stable.

A Study of Experiments in Improving Science Literacy and Disciplinary Behavior of 6 Year-Old Children (Processing Organic Waste Using Black Soldier Fly Magot)

Early childhood needs to process an understanding of natural phenomena and the world around them. Children are not yet aware of environmental problems because they are developing various skills such as science literacy skills and mindsets that are reflected in disciplinary attitudes. The purpose of the study was to determine the significance of increasing science literacy and disciplinary behavior of 6-year-old children by processing organic waste using black soldier fly maggots. The research method uses a quantitative approach through a quasi-experimental study process with the research from Nonequivalent Control Group Design. The results of theoretical research based on the comparison between the t count and t table show the t count of 3.792&gt; 2.306, and the significant result is 0.001 &lt;0.05, then Ha is accepted. So it is said that there is an increase in science literacy skills and disciplinary behavior of children aged 6 years, through organic waste processing experiments using black soldier fly maggot.

Improved nutritional and antioxidant properties of black soldier fly larvae reared on spent coffee grounds and blood meal by-products

Black Soldier Fly larvae (BSFL) are a promising and sustainable alternative to obtain proteins. Due to their high growth rate and ability to use different substrates as feeding stocks, BSFL can be also used to valorize food waste. Thus, the aim of this research was to unravel the potential use of Spent Coffee Grounds (SCG) and blood meal alone or mixed as feedstocks for BSFL and the nutritional changes for BSFL meal, especially after simulated human in vitro digestion and fermentation. Chicken feed was used as a control. Chicken feed showed the highest BSFL growth (P < 0.05) compared with blood meal and the mix made of blood meal and SCG; the latter caused the lowest growth. The meal obtained from BSFL fed with blood meal had the highest protein content, as well as the highest levels of short chain fatty acids (SCFAs) produced after in vitro fermentation by the human gut microbiota. On the other hand, the meal from larvae fed with SCG showed higher antioxidant capacity than the others in the DPPH, FRAP and ABTS assays. The digestibility of macronutrients, release of antioxidant capacity and production of SCFAs of the BSFL meal were improved when using these substrates, compared to chicken feed.

Wastewater treatment using black soldier fly larvae: Influence of larval density and solid support

The use of Black Soldier Fly larvae for wastewater treatment has emerged as a promising alternative, coupling high removal efficiency with recovery of high-value products. To overcome the critical issue of larvae mortality in a liquid environment, a patented solution employs a physical porous support to be saturated by liquid, allowing larvae mobility. In view of process optimisation, a knowledge gap persists regarding both the optimal supporting material and larval density. The optimum supporting material and larval density should maximise the porosity (i.e. active volume of the reactor) and the removal efficiencies respectively, without compromising the survival rate. In the present study, three different plastic products (granules, pall rings and geomat) were tested at four different larval densities (4, 8, 16 and 32 larvae/cm2). Process performance was compared in terms of larval growth and substrate removal. An increase in larval density only impairs process once threshold density is exceeded. The threshold depended on the supporting material: higher porosity materials allow the use of higher larval densities, and therefore higher organic loads, with reduced reactor volumes.The best performance was achieved at larval density of 16 larvae/cm2 with pall rings, resulting in a survival rate and carbon removal efficiency of approx. 92 % and 97 % respectively.

Rearing of Black Soldier Fly Larvae with Corn Straw and the Assistance of Gut Microorganisms in Digesting Corn Straw.

Corn straw is considered a renewable biomass energy source, and its unreasonable disposal leads to resource waste and environmental pollution. Black soldier fly (Hermetia illucens L.) larvae (BSFL) facilitate the bioconversion of various types of organic wastes. In this study, we found that 88% of BSFL survived, and 37.4% of corn straw was digested after 14 days of feeding with corn straw. Contrary to expectations, the pretreatment of corn straw with alkaline hydrogen peroxide did not promote its digestion but rather reduced the growth and survival rates of BSFL. Acinetobacter, Dysgonomonas, and unclassified Enterobacteriaceae were the abundant genera in the BSFL gut fed with corn straw. Compared with the standard diet, the relative abundances of carbohydrate metabolism genes, such as the gene abundances of β-glucosidase and α-glucosidase, were higher with corn straw as the substrate. These results suggested that the gut microbial community could regulate suitable and functional microorganisms in response to the substrates. Furthermore, four cellulase-producing strains, namely Klebsiella pneumoniae, Proteus mirabilis, Klebsiella oxytoca, and Providencia rettgeri, were isolated from the guts of corn straw BSFL. These four strains helped increase the conversion rates of corn straw, the weights of BSFL, and survival rates. In summary, we reared BSFL with corn straw and discovered the functions of gut microorganisms in adapting to the substrates. We also isolated four cellulase-producing strains from the BSFL guts and declared the benefits of BSFL digesting corn straw.

Oil-in-water emulsion stabilized by hydrolysed black soldier fly larvae proteins: Reproduction of experimental data via phase-field modelling

A phase field model based on the Cahn-Hilliard equation was validated experimentally by comparison of the numerical data with experimental data of emulsions of oil in water stabilized with Black Soldier Fly Larvae Proteins (BSFLP) and protein hydrolysates. Among the model parameters, the surface tension was determined by the pendant drop method, and the mobility by two different experiments, one based on the Turbiscan Stability Index, and another one based on the history of the average d3,2, both of them throughout a 48 h period. The initial condition was built from an experimental droplet size distribution measured prior to phase separation. Results show that the model is able to quantitatively reproduce the phase separation kinetics, and provide an intuitive graphical representation of the droplet growth. Application of this procedure to other systems will allow the generalization of phase field modelling as a predictive tool for food applications.

A superior chitin product: Black soldier fly larvae chitin, beneficial to growth performance, muscle quality and health status of largemouth bass Micropterus salmoides, in comparison to shrimp chitin

We appraised the effects of black soldier fly larvae chitin (BSFC) with four levels (0.4 %, 0.8 %, 1.2 % and 1.6 %) and shrimp chitin (SC) with three levels (0.4 %, 0.8 % and 1.6 %) in diets on the growth performance, nutrient composition and health status of largemouth bass (8.00 ± 0.29 g) fed for 56 days. The results showed that the dietary 0.4 % BSFC distinctly increased the specific growth rate and weight gain rate of fish (P < 0.05), and evidently promoted the deposition of C22:1n-9, C24:1n-9 and increased total monounsaturated fatty acid content in the muscle (P < 0.05). BSFC and SC distinctly stimulated the secretion of β-N-acetylglucosaminidase in the hindgut (P < 0.05). The antioxidant status of fish was improved by BSFC and SC, which was attributed to the decreased malonaldehyde contents in the muscle and midgut and the increased total superoxide dismutase and catalase activities in the midgut. The 1.2 %–1.6 % BSFC and 0.8 %–1.6 % SC enhanced the non-specific immunity of fish, which was attributed to the marked promotion of lysozyme, acid phosphatase and alkaline phosphatase activities by BSFC and the distinct enhancement of lysozyme activity by SC in the serum or midgut (P < 0.05). BSFC and SC notably reduced the abundance of potential pathogenic bacterium Vibrio and Photobacterium (P < 0.05), and 0.4 %, 1.2 %, 1.6 % BSFC and 0.4 % SC markedly increased the abundance of probiotic bacterium Cetobacterium (P < 0.05), and the BSFC and SC of 0.8 % significantly increased the abundance of the probiotics Bifidobacterium and Bacillus respectively (P < 0.05). The increase of acetic acid, propionic acid, butyric acid and valeric acid concentrations in the hindgut might be related to the increase of intestinal probiotics, and 0.8 %–1.6 % BSFC and 0.4 % SC obviously increased the total short-chain fatty acid levels in the hindgut (P < 0.05). BSFC and SC remarkably reduced muscle fiber area and diameter (P < 0.05), while fiber densities were significantly increased under 0.4 %, 0.8 % BSFC and 0.4 % SC supplementation (P < 0.05), which was accordant with increased mRNA expression levels of myod, myf-5, smad-2, tgf-β1 and decreased mRNA expression levels of myos in the muscle. Collectively, BSFC and SC had no negative effect on the growth of largemouth bass, and improved the health status of fish. The dietary 0.4 % BSFC supplementation was beneficial to fish growth and muscle quality, and BSFC was a superior chitin product in comparison to SC.

Potential Utilization of Defective Fruit Juice Products for Black Soldier Fly Cultivation to Reduce Waste Generation in the Beverage Industry

The waste of fruit juice and tea defective products has organic content that conventionally has the potential to be utilized as a medium or place for maggot growth, but until now it has not been equipped with supporting scientific studies. Therefore, the objectives of the study are: 1) Assessing the characteristics of fruit juice and tea product waste, 2) Assessing the effectiveness of maggot in reducing fruit juice and tea product waste. This research is experimental in nature using variations in the composition of fruit juice and tea product waste as a source of nutrition for maggot which is carried out for 12 days. This variation uses the Complete Randomized Design method with 3 (three) treatments, namely variations in the composition of 60% tea + 40% fruit juice waste (S1), 50% tea + 50% fruit juice waste (S2), and 40% tea + 60% fruit juice waste (S3) in duplo. In addition, this study was completed with a control treatment. The results showed that the characteristics of fruit juice and tea waste containing protein (3.78-4.59%), fat (0.90-1.31%), carbohydrates (28.15-29.15%), crude fiber (2.42-3.12%) can be used as maggot cultivation media. The highest protein and crude fiber contents were found in the S3 composition waste at 4.59% and 3.12%. Maggot cultivation results that showed the highest fat and carbohydrate content were found in S1 composition waste at 1.31% and 29.15%. The recommended composition variation is S1 composition with the highest substrate consumption value and waste reduction index of 57.47% and 20.06%.