The solar salterns of Khambhat, Gujarat are distinct biological niches distinguished by high salinity levels and harsh environmental circumstances. Our goal in this work was to isolate and characterise bacteria in order to investigate the diversity of microbes found in these saline soils. Standard microbiological procedures were used to isolate the bacteria from samples taken from various depths of the solar saltern ponds. To clarify their taxonomic identity and physiological characteristics, the isolated bacterial strains underwent morphological, biochemical and molecular characterization. Catalase, oxidase and fermentation assays are examples of biochemical studies that shed light on the isolated strains' metabolic capacities. Moreover, the phylogenetic relationships of the isolated bacteria were ascertained through molecular characterization using 16S rRNA gene sequencing. This work advances our knowledge of the microbial ecology of hypersaline environments and highlights the potential uses of bacteria isolated from these settings in biotechnology such as bioremediation, the synthesis of enzymes and the identification of new bioactive substances. To fully utilize these bacteria's biotechnological potential, more research into their functional properties is necessary.