Abstract The semi-continuous digestion experiment of food waste was carried out based on a solar-assisted heat anaerobic digestion reactor. The effects of organic load regulation (OLR of 2.0, 4.0, 6.0 and 7.0 kg m−3 d−1 VS (volatile solid)) on methane production, physical and chemical parameters, and microbial community structure were studied. The results showed that 6.0 kg m−3 d−1 VS could achieve the optimal methane production as the anaerobic digestion limit OLR of kitchen waste. At this stage, the accumulation of organic acids in the reactor led to a great fluctuation of pH. Artificial alkali regulation could restore the stable operation of the reactor, but the reactor could not withstand the OLR shock of 7.0 kg m−3 d−1 VS. In addition, Qualcomm sequencing results showed that microorganisms showed high functional concentration and poor community richness under low OLR, and increasing OLR could promote microbial richness. At the same time, the Methanosaeta of acetic acid methanogens was relatively abundant in the low OLR stage, while the hydrogen trophic methanogenic bacteria Methanoregula and Methanospirillum showed high activity in the high OLR stage, but the excessive reproduction of acidogenic bacteria in the digestive system would affect the stability of the archaea community when the OLR was too high.