Soil Phosphatase Research Articles

Phosphorus scarcity contributes to nitrogen limitation in lowland tropical rainforests.

There is increasing evidence to suggest that soil nutrient availability can limit the carbon sink capacity of forests, a particularly relevant issue considering today's changing climate. This question is especially important in the tropics, where most part of the Earth's plant biomass is stored. To assess whether tropical forest growth is limited by soil nutrients and to explore N and P limitations, we analyzed stem growth and foliar elemental composition of the five stem widest trees per plot at two sites in French Guiana after 3 years of nitrogen (N), phosphorus (P), and N + P addition. We also compared the results between potential N-fixer and non-N-fixer species. We found a positive effect of N fertilization on stem growth and foliar N, as well as a positive effect of P fertilization on stem growth, foliar N, and foliar P. Potential N-fixing species had greater stem growth, greater foliar N, and greater foliar P concentrations than non-N-fixers. In terms of growth, there was a negative interaction between N-fixer status, N + P, and P fertilization, but no interaction with N fertilization. Because N-fixing plants do not show to be completely N saturated, we do not anticipate N providing from N-fixing plants would supply non-N-fixers. Although the soil-age hypothesis only anticipates P limitation in highly weathered systems, our results for stem growth and foliar elemental composition indicate the existence of considerable N and P co-limitation, which is alleviated in N-fixing plants. The evidence suggests that certain mechanisms invest in N to obtain the scarce P through soil phosphatases, which potentially contributes to the N limitation detected by this study.

The effects of feeding with organic waste by terrestrial isopod Philoscia Muscorum on enzyme activities in an incubated soil

Soil fauna are important biological factors that affect litter decomposition and play an important role in the release of nutrients and improve soil enzyme activities. This study focused on the effects of isopods on enzymatic activities of soil. Lab experiments were conducted to assess the influence of terrestrial isopod Philoscia Muscorum on enzyme activities during the incubation. In Lab experimental food sources from wheat straw were prepared. Dehydrogenase, urease, alkaline phosphatase and arylsulphatase activity in soil treated with different number of isopods with wheat straw were determined in 28 days incubation. Results showed that the presence of isopods significantly increased (P

Agronomic response, physiological and bio-chemical composition of transplanted rice influenced by nutrient management practices

Field experiment was conducted at wetland farm of Tamil Nadu Agricultural University, Coimbatore during kharif season to study the effect of integrated nutrient management practices on transplanted lowland rice. The experiment was laid out in randomized block design with three replications and nine treatments. Rice CO(R) 48 was used as a test variety. Based on N equivalent basis, required quantities of dhaincha, vermicompost, farmyard manure were incorporated before transplanting of rice. The P and K requirement was supplied separately through inorganic sources as per treatment schedule. The results revealed that yield attributes (number of productive tillers m-2 (288), total number of grains panicle-1 (217), percentage of filled grains (89.4) and yield (6248 kg ha-1), panicle parameters (panicle length (28.3 cm), panicle weight (4.64 g ), test weight (18.1 g), physiological and bio chemical parameters (chlorophyll index (36.02), chlorophyll stability index (72.22 %), relative water content (78.43%), soluble protein content (15.58 mg g-1) were significantly influenced with application of 100% NPK through inorganic fertilizers + 6.25 t dhaincha followed by application of 100% N through dhaincha + balance P & K through inorganic fertilizers. Maximum soil enzyme dynamics (urease activity (47.1 µg NH+ g-1 soil 24 h-1), soil dehydrogenase activity (37.1 µg of TPF released g-1 of soil 24 h-1), soil phosphatase activity (39.1 µg of p - nitrophenol released g-1 of soil 24 h-1), nitrogen use efficiency (agronomic efficiency, physiological efficiency and apparent N recovery) and chemical composition like protein (7.41 %), carbohydrates (78.53%), amylose content (27.07 %), fat (0.58 %) and fibre (0.231 %) were high with application of 100 % N through dhaincha + balance P & K through inorganic fertilizers.

Symbiotic nitrogen fixation does not stimulate soil phosphatase activity under temperate and tropical trees.

Symbiotic nitrogen (N)-fixing plants can enrich ecosystems with N, which can alter the cycling and demand for other nutrients. Researchers have hypothesized that fixed N could be used by plants and soil microbes to produce extracellular phosphatase enzymes, which release P from organic matter. Consistent with this speculation, the presence of N-fixing plants is often associated with high phosphatase activity, either in the soil or on root surfaces, although other studies have not found this association, and the connection between phosphatase and rates of N fixation-the mechanistic part of the argument-is tenuous. Here, we measured soil phosphatase activity under N-fixing trees and non-fixing trees transplanted and grown in tropical and temperate sites in the USA: two sites in Hawaii, and one each in New York and Oregon. This provides a rare example of phosphatase activity measured in a multi-site field experiment with rigorously quantified rates of N fixation. We found no difference in soil phosphatase activity under N-fixing vs. non-fixing trees nor across rates of N fixation, though we note that no sites were P limited and only one was N limited. Our results add to the literature showing no connection between N fixation rates and phosphatase activity.

Seaweed extract enhances drought resistance in sugarcane via modulating root configuration and soil physicochemical properties

Seaweed extract is widely used in sustainable agricultural management and can promote sugarcane growth even under low-water availability conditions. However, to date, little information has been gained regarding the role of seaweed extract in regulating sugarcane root configuration and soil physicochemical properties under drought conditions. In this study, the effects of seaweed extract on soil physicochemical properties of sugarcane were studied by soil culture experiments, and the effects of seaweed extract on root architecture of different sugarcane varieties “YT03–393” (drought-sensitive variety) and “cv. ROC22” (drought-resistant variety) were explored by hydroponic experiments. The results revealed that the application of seaweed extract increased the proportion of aggregates > 0.25 mm in the soil and increased the moisture content of the soil under water deficiency conditions. Furthermore, the seaweed extract application increased the activities of urease, sucrase, acid phosphatase, and glucosidase in soil and increased the soil contents of available nutrients such as N, P, and K. Under drought stress, seaweed extract promoted root growth in deeper soil. The proportions of root dry weight and root length density in the soil of 20–40 cm depth increased by 45.0% and 9.8%, and 38.3% and 8.9% in cv. YT03–393 and cv. ROC22, respectively. Additionally, seaweed extract application improved the activities of antioxidant enzymes, including SOD, CAT, and POD, in roots and alleviated the oxidative damage of roots caused by drought stress. It can be concluded that seaweed extract application can improve soil physical structure, promote enzyme activities, and increase the available N, P, and K of the soil. Furthermore, seaweed extract can promote root growth, change root architecture, and improve the water use efficiency of roots, thus improving drought resistance of sugarcane plants.

Response to Drought Stress of In Vitro and In Vivo Propagated Physalis peruviana L. Plants Inoculated with Arbuscular Mycorrhizal Fungi

Physalis peruviana L. is one of the most favorable tropical fruit due to its fast growth and nutritional properties. The current research outlined the response to drought stress of golden berry plants inoculated with arbuscular mycorrhizal fungi Claroideoglomus claroideum and propagated in vitro as well as from seeds. The implementation of mycorrhizal symbiosis was determined by root colonization, glomalin content, and alkaline and acid phosphatases in roots and soil. The plant protection was assured by enzyme and non-enzyme antioxidants. The adapted in vitro propagated plants demonstrated higher resistance to drought than plants developed from seeds indicated by increased growth parameters (shoot, root biomass, fruit number), plastid pigment content, antioxidant activity, and less enhance mentofoxidative markers levels in water-deficient conditions. The findings in the present research are relevant to obtain the optimal mycorrhizal association and type of propagation in an adverse environment for golden berry development and will lead to the establishment of a database and model of varied plant responses to stressful conditions such as drought.

Arsenic mobility and toxicity on soil acid phosphatase activity increased with added phosphorus in contaminated mine soils

AbstractSoil phosphatase activity is identified as sensitive to arsenic (As) pollution and therefore, often used as an indicator to estimate As toxicity in soil. Nevertheless, the effect of As on soil phosphatase activity after its desorption by P is not adequately studied. This study employed sequential extraction procedure and ecological dose parameters to show As mobility and its toxicity to acid phosphatase (AcP). Results indicated that P application significantly increased As desorption ratio in the As ageing process. In S1 (As 2895 mg kg−1) soil, after P addition, the water‐soluble (FWS) fraction had the highest proportion of As, while for S2 (As 645 mg kg−1) and S3 (As 39 mg kg−1) it was residual (FRES) fraction. Additionally, P addition increased the As bioavailability in all soils. Generally, P addition significantly reduced soil AcP activity under all P concentrations and increased in the soil with minimum As content. The variation partitioning analysis indicated that for S1, P concentration was the major factor, for S2 and S3 soils, it was As desorption and ageing time, respectively, that influenced the AcP activity in soils. The calculated ecological dose (ED50) showed that in most cases, As toxicity increased at the end of ageing. Thus, it is essential to control the phosphorus fertilizer applications in silty soils with acid and neutral pH, since it can lead to increased As mobility and toxicity to soil AcP.

Exploring the Potential of White-Rot Fungi Exudates on the Amelioration of Salinized Soils

Soil salinization has a major impact on crop revenue, which may be counteracted by harnessing the microbiota that the soil itself harbors. This study aimed to explore the application of fungi exudates (Trametes versicolor and Pleurotus sajor caju) on the potential relief of salt stress in the performance of Lens culinaris in two different soils (a silvopastoral agroforestry soil and LUFA 2.2). Three salinity levels (8, 16, and 26 mS·cm−1) and three levels of each fungal exudate (1%, 6%, and 12%) were evaluated in a 96-h cross-design experiment. Seed germination was checked daily, and after 96-h, seedling radicle and hypocotyl were measured, along with fresh and dry weights and soil phosphatase activity. The sole application of exudates from neither fungus affected the germination and seedling development of L. culinaris. Salinity alone induced a dose-effect response in all L. culinaris tested endpoints, as expected because conductivities above 8 mS·cm−1 are known to be detrimental for many crop species. Application of exudates to salinized soils improved germination rates at 16 and 26 mS·cm−1 compared to the single respective salinity treatment alone, but mainly in assays carried out in natural soil. In addition, under the same conditions (26 mS·cm−1 and all fungi %), there was an improvement in natural soil acid phosphatase activity. The application of rot fungi exudates demonstrates potential in what might prove to be a sustainable measure to reclaim salinized soils and improve crop productivity, which is consistent with current soil protection policies.

Effects of the dual inoculation of dark septate endophytes and Trichoderma koningiopsis on blueberry growth and rhizosphere soil microorganisms.

Blueberry is a shallow root plant in which the absorption of nutrients is inefficient, resulting in slow growth under artificial cultivation conditions. Endophytes play an important role in promoting plant growth; however, the effects of Trichoderma spp. and dark septate endophytes (DSEs) on host plant growth and soil microorganisms are still debatable. We isolated two endophytic fungal species, Trichoderma koningiopsis (TK) and a DSE (Amesia nigricolor; AN), from blueberry roots, which can solubilize insoluble phosphorus and produce amylase and cellulase to promote plant growth. We found that under dual inoculation, the colonization rate and colonization intensity of TK were higher than they were under single inoculation with TK, while the colonization rate and colonization intensity of AN were lower under dual inoculation than under single inoculation with AN. The plant nutrients, root activity, available potassium, and parts of soil phosphatase activities were highest under dual inoculation. TK inoculation resulted in the highest diversity and richness in the soil fungi and bacteria, followed by dual inoculation. The abundance of Ascomycota, Acidobacteriae, Firmicutes, and Actinobacteriota increased significantly, resulting in Trichoderma and Vicinamibacteria inoculated with TK, Chaetomium and Alicyclobacillales inoculated with AN, and Hypocreales and Burkholderiaceae with dual inoculation enriched in the soil.

Effect of biogas slurry returning to field on soil phosphatase activity

Effect of biogas slurry returning to field on soil phosphatase activity

Effects of long-term irrigation on soil phosphorus fractions and microbial communities in Populus euphratica plantations.

Irrigation has been demonstrated to be effective in managing Populus euphratica plantations, but its impacts on phosphorus (P) availability and the soil microbiome have not been fully elucidated. In this study, we compared soil properties, P fractions, phosphatase activities, and microbial communities in the surface soil (0-20 cm) of P. euphratica plantations under both drought and irrigation conditions. We found that total P, labile P and moderately labile P all increased significantly under irrigation by 12.3%, 70.1%, and 3.0%, respectively. The increased levels of labile P were primarily driven by higher levels of NaHCO3-Pi, which increased from 1.9 to 12.3 mg·kg-1. Furthermore, irrigation markedly altered labile P composition and the relative levels of resin P, NaHCO3-Pi, and NaHCO3-Po were all impacted. Improved soil moisture increased soil phosphatase activity, suggesting that soil organic P (Po) mineralization was positively affected by irrigation. Moreover, we observed that bacterial diversity, fungal diversity, and alkaline phosphatase gene communities, rather than total microbial biomass carbon or total phospholipid fatty acids, were most explained in the dynamics of soil P fractions. Furthermore, we found positive correlations among inorganic P (Pi) and Bradyrhizobiaceae, Nocardiaceae, and Sphingomonadaceae, whereas negative correlations were found between Burkholderiaceae and Pi, highlighting the diverse functional bacteria involved in P cycling. Our study demonstrates that irrigation can increase soil P availability and supply capacity, with shifts in P composition closely linked to changes in soil microbial characteristics. Water management strategies that target the restoration of soil microbial communities may therefore improve soil quality and enhance soil P cycling.

Penicillium oxalicum augments soil lead immobilization by affecting indigenous microbial community structure and inorganic phosphate solubilization potential during microbial-induced phosphate precipitation

Phosphate-solubilizing microorganisms (PSMs) are critically important for increasing soil phosphate (P) and decreasing lead (Pb) bioavailability during microbial-induced phosphate precipitation (MIPP). However, their relative contributions to the indigenous soil microbial communities and P-cycling genes during the MIPP process remain unclear. In this study, inoculation of the PSM P. oxalicum in hydroxyapatite-cultured and Pb-contaminated soil increased soil phosphatase activities, available P (AP) concentrations and reduced available Pb levels. Metagenomics revealed a 3.9–44.0% increase in the abundance of P-cycling genes by P. oxalicum inoculation. No P-cycling genes were assigned to Penicillium. While P. oxalicum increased the complexity of microbial community co-occurrence networks, and improved the directly interrelationships between Penicillium and genera containing P-cycling gene. These results suggesting that P. oxalicum obviously positively affected the regulation of indigenous P-cycling functional communities during the MIPP process. Inorganic P solubilization genes (gcd, ppa, and ppx) have been shown to affect soil AP, suggesting that inorganic P solubilization is the major driver of Pb immobilization improvement following P. oxalicum inoculation. These results enhance our understanding of the significant ecological role of PSMs in governing soil P-cycling and alleviating Pb2+ biotoxicity during the MIPP process.

Leaf and Soil Phosphatases and the Correlations with Leaf P Resorption of Lucerne Under P Fertilization and Irrigation

Leaf and Soil Phosphatases and the Correlations with Leaf P Resorption of Lucerne Under P Fertilization and Irrigation

A core microbiome in the hyphosphere of arbuscular mycorrhizal fungi has functional significance in organic phosphorus mineralization.

The mycorrhizal pathway is an important phosphorus (P) uptake pathway for more than two-thirds of land plants. The arbuscular mycorrhizal (AM) fungi-associated hyphosphere microbiome has been considered as the second genome of mycorrhizal P uptake pathway and functionality in mobilizing soil organic P (Po). However, whether there is a core microbiome in the hyphosphere and how this is implicated in mining soil Po are less understood. We established on-site field trials located in humid, semiarid, and arid zones and a microcosm experiment in a glasshouse with specific AM fungi and varying soil types to answer the above questions. The hyphosphere microbiome of AM fungi enhanced soil phosphatase activity and promoted Po mineralization in all sites. Although the assemblage of hyphosphere microbiomes identified in three climate zones was mediated by environmental factors, we detected a core set in three sites and the subsequent microcosm experiment. The core members were co-enriched in the hyphosphere and dominated by Alphaproteobacteria, Actinobacteria, and Gammaproteobacteria. Moreover, these core bacterial members aggregate into stable guilds that contributed to phosphatase activity. The core hyphosphere microbiome is taxonomically conserved and provides functions, with respect to the mineralization of Po, that AM fungi lack.

Beneficial shift of rhizosphere soil nutrients and metabolites under a sugarcane/peanut intercropping system.

Intercropping systems have been studied as a sustainable agricultural planting pattern to increase soil quality and crop yields. However, the relationships between metabolites and soil physicochemical properties remain poorly understood under sugarcane/peanut intercropping system. Thus, we determined the rhizosphere soil physicochemical properties, and analyzed rhizosphere soil metabolites and root metabolites by metabolomics method under monoculture and intercropping patterns of sugarcane and peanut. The results showed that pH, the contents of total phosphorus (P), total potassium (K), available nitrogen (N), available phosphorus (P), and available potassium (K) were higher in rhizosphere soil of intercropping peanut than monoculture peanut, and the content of total P was higher in rhizosphere soil of intercropping sugarcane than monoculture sugarcane. Sugarcane/peanut intercropping also significantly increased the activities of acid phosphatase and urease in rhizosphere soil. The metabolomics results showed that 32 metabolites, mainly organic acids and their derivatives (25.00%), nucleotides and their metabolites (18.75%), were detected in root and rhizosphere soil samples. In the MP-S (rhizosphere soil of monoculture peanut) vs. IP-S (rhizosphere soil of intercropping peanut) comparison, 47 differential metabolites (42 upregulated) were screened, including glycerolipids (19.15%), organic acids and their derivatives (17.89%), and amino acids and their metabolites (12.77%). In the MS-S (rhizosphere soil of monoculture sugarcane) vs. IS-S (rhizosphere soil of intercropping sugarcane) comparison, 51 differential metabolites (26 upregulated) were screened, including heterocyclic compounds (15.69%), glycerolipids (11.76%), and organic acids and their derivatives (9.80%). The metabolite species from MP-S, MS-S, IP-S, and IS-S were similar, but some metabolite contents were significantly different, such as adenine, adenosine, maltotriose, thermozeaxanthin-13 and PE-NMe (20:0/24:0). Adenine and adenosine were detected in root and rhizosphere soils, and their levels were increased in the intercropping treatment, which were mainly related to enhanced purine metabolism in root and rhizosphere soils under the sugarcane/peanut intercropping system. Importantly, adenine and adenosine were significantly positively correlated with total P and total K contents, acid phosphatase and urease activities, and pH. This study clarified that the sugarcane/peanut intercropping system could improve soil nutrients and enzymes and was related to purine metabolism.

Soil phosphatase assay to evaluate arsenic toxicity should be performed at the soil's actual pH

Soil phosphatase is considered an indicator to assess soil arsenic (As) pollution. In the phosphatase activity determination, a fixed buffer value (pH 5–10) is commonly used for all soils, ignoring the soil's actual pH. Here, we determined the soil phosphatase activity of 20 soils under As stress at the soils' pH, and the As inhibition mechanism was also explored by the enzyme kinetics. Our results show that soil phosphatase activity was significantly inhibited under As stress. The inhibition rate in acid soils (39.2 %) was considerably higher than in alkaline soils (25.4 %) when As concentration was 600 mg kg−1. For alkaline soils, As inhibited phosphatase by competitive inhibition or linear mixed inhibition, while for acid soils, it was more complex, including linear mixed inhibition, non-competitive inhibition, and anti-competitive inhibition. Simultaneously, our results showed that the ecological dose (ED10) described by the partial inhibition model was far below than the complete inhibition model. According to the partial inhibition model, the ED10 of As ranged from 2.66 to 164.07 mg kg−1 for alkaline soils and 0.11 to 89.95 mg kg−1 for acid soils. Moreover, Vmax/Km of phosphatase is a more sensitive index for evaluating As contamination than Vmax in partial inhibition models. The ED10 obtained based on the relationship between Vmax/Km and As concentration was 0.64–34.75 mg kg−1 for acid soils and 8.48 to 20.16 mg kg−1 for alkaline soils. This also confirms Vmax/Km as a sensitive and ideal index for assessing As pollution under soils' actual pH. Furthermore, soil pH and cation exchange capacity are dominant factors affecting As inhibition on soil phosphatase. The above kinetic studies indicate that performing the assay by adjusting the buffer pH to the soil pH is essential for more accurately evaluating arsenic toxicity.

Metabolic characterization of phosphate solubilizing microorganisms and their role in improving soil phosphate solubility, yield of upland rice (Oryza sativa L.), and phosphorus fertilizers efficiency

Phosphate solubilizing microbes (PSM) can improve soil P availability by P dissolution. These microbes can make substances that regulate plant growth, which promotes plant growth. The present study aimed to characterize PSM and determine how PSM application affected P solubilization, soil phosphatase activity, and upland rice yield. The greenhouse experiment used a factorial randomized block design (RBD) with two factors and three replications. The first factor was PSM isolates, which came in four different forms: without microbes, with microbes (Burkholderia sp.), with fungus (Penicillium sp.), and with a combination of microbes (Burkholderia sp. and Penicillium sp.). The PSM isolates were characterized to analyze the production of organic acids, phosphatase enzymes, and phytohormones. The second factor was the superphosphate fertilizer dose, which has four levels: 0, 50, 75, and 100 kg P ha−1. According to the PSM characterization, it produced organic acids such as lactate acid, oxalate acid, citric acid, and acetate acid, as well as phytohormones (IAA) and the enzyme phosphatase. The pot experiment results show that the PSM inoculation raised the available P and soil phosphatase, P content of the plant, decreased soil organic P, and increased upland rice production. For improving available P, phosphatase activity, P content of the plant, and upland rice yields, mixed inoculants of phosphate-solubilizing bacteria and fungi performed better. The availability of soil P, the activity of the enzyme phosphatase, and the upland rice yields were all improved by applying P fertilizer at 75 kg P ha−1. This study showed that PSM as a biofertilizer reduced the dosage of inorganic fertilizers by up to 25%.

Soil phosphorus availability and utilization are mediated by plant facilitation via rhizosphere interactions in an intercropping system

We hypothesized that soil phosphorus (P) utilization might be tightly associated with interspecific interactions. To clarify this issue, a maize-grass pea intercropping system was investigated in a rainfed field with P addition (P0 and P +), water (rain-fed and plastic film mulching) and isolation (root barrier and root intermingling) treatments. The data indicated that total net effect was higher than 0 under all the conditions, displaying facilitative effects of biodiversity on agroecosystem productivity. The dynamics of total land equivalent ratio demonstrated that the intensity of plant-plant facilitation became greater under P-deficient condition. Maize was the facilitated species consistently, while grass pea changed from facilitated species under low soil P and moisture to facilitator under high soil P and moisture. The driving mechanism was associated with soil acidification and microbial community promotion effect under P-deficient condition. Intercropped rhizosphere soil phosphatase activity was increased by 4.9–17.2%, and Olsen-P availability was evidently enhanced relative to monoculture. Interestingly, drought stress significantly enhanced this trend. Critically, root isolation confirmed that plant-plant facilitation was briefly driven by interspecific rhizosphere interactions. Conclusively, this study confirmed the context-dependent facilitation shift in agroecosystem and provided a novel insight into the importance of rhizosphere interaction on soil P availability and utilization.

Potential Application of Paenibacillus sp. C1 to the Amelioration of Soda Saline-Alkaline Soil

In this study, a saline-alkaline-tolerant bacterial strain named Paenibacillus sp. C1 was screened and isolated, and its basic properties were characterized. Its phosphorus dissolution, organic acid production, and application on ameliorating soda saline-alkaline soil were studied, and the changes of microbial communities after adding strain C1 with or without rice straws, (NH4)2SO4 and Ca3(PO4)2 were investigated. The results demonstrated that when the nitrogen, carbon, and phosphorus sources were (NH4)2SO4, glucose, and Ca3(PO4)2, respectively, strain C1 was in the best phosphorus-dissolving condition. The strain had good characteristics of acid production and phosphorus dissolution under the condition of high salinity and alkalinity. It could secrete cellulases, decompose rice and corn straws, and produce polysaccharides effectively. Strain C1 [especially with rice straws, (NH4)2SO4 and Ca3(PO4)2] could increase the content of available phosphorus (AP), organophosphorus (OP), total organic carbon (TOC), and polysaccharide (PS) and the activity of alkaline phosphatase (ALP) in saline-alkaline soil. Furthermore, adding strain C1 [especially with rice straws, (NH4)2SO4 and Ca3(PO4)2] could alter the bacterial flora of soil to make it adapt to high salinity and alkalinity better. Our study can be an important step toward the application of Paenibacillus sp. C1 to the amelioration of soda saline-alkaline soil and the investigation of its deep mechanisms.

Responses of Bacterial Community Structure, Diversity, and Chemical Properties in the Rhizosphere Soil on Fruiting-Body Formation of Suillus luteus.

Mycorrhiza helper bacteria (MHB) play an important role in driving mycorrhizal formation. There are few reports on the relationship between bacteria and fruiting growths. Taking mycorrhizal rhizosphere soil from sporocarps of the S. luteus and non-mycorrhizal rhizosphere soil of the host plant (Larix gmelinii), we measured the bacterial community structure and diversity and chemical properties to clarify the effect of bacteria on fruiting-body formation. The bacterial diversity was significantly higher in mycorrhizal rhizosphere soil (p < 0.05) than that in non-mycorrhizal rhizosphere soil. The relative abundance of Burkholderia, Bradyrhizobium, Pseudomonas, and Rhizobium was significantly higher (p < 0.05) in mycorrhizal rhizosphere soil than in non-mycorrhizal rhizosphere soil. The soil organic matter (SOM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), ammonium nitrogen (AN), available phosphorus (AP), available potassium (AK), and the activity of catalase, urease, and phosphatase in mycorrhizal rhizosphere soil were significantly higher (p < 0.05) than those in non-mycorrhizal rhizosphere soil. A redundancy analysis (RDA) showed that dominant bacteria are closely related to soil enzyme activity and physicochemical properties (p < 0.05). The boletus recruits a large number of bacteria around the plant roots that speed up nutrient transformation and increase the soil nutrient content, providing an important guarantee for mycelium culture and fruiting-body formation. These findings provide ideas for the nutritional supply of boletus sporocarps and lay the theoretical foundation for the efficient artificial cultivation of boletus.