Soil Organic Carbon Fractions Research Articles

Disentangle the drivers of soil organic carbon mineralization and their temperature sensitivity in both topsoil and subsoil: Implication of thermal stability and chemical composition

Information about the temperature dependence of soil organic carbon (SOC) mineralization is important to predict the possible carbon (C) release from the terrestrial ecosystem facing global warming. However, the way in which SOC quality and mineral protection of SOC affects mineralization rates and their response to increasing temperature remains poorly understood. Here, we focused on investigating thermal stability and chemical composition of soils which differed greatly with the type and extent of organo-mineral associations, and revealing their implications for SOC mineralization. Chemical composition and thermal stability of SOC were analyzed with 13C nuclear magnetic resonance spectroscopy (13C NMR) and simultaneous thermogravimeteric analysis and differential scanning calorimetry (TG-DSC), respectively. Soil mineralization and its temperature sensitivity (Q10) were assessed by incubating soils at 15 and 25 °C for 90 days.Our results showed that SOC of subsoil was more biologically stable, but exhibited higher temperature sensitivity than those of topsoil. Significant differences in chemical composition and thermal stability of SOC were detected across soils developed from four parent materials but not between two soil layers, and indicators obtained from the two techniques showed apparent associations with depth-specific SOC mineralization rates. Increase in SOC mineralization rates at two incubation temperatures and two soil layers were observed when the soils were characterized by higher percentage of labile SOC fractions (i.e., O-alkyl C and organic carbon oxidized during exothermic reactions). The Q10 values of topsoil were positively and mostly related to the percentage of Alkyl C. In addition to notable relationships with chemical composition, the Q10 values of subsoil were positively correlated with Exo1/Exo2 ratios and Energy density values, and negatively correlated with TG-50. It was interesting to note that thermal stability was possibly as a function of soil texture and total oxides. Results from VPA analysis further revealed that SOC mineralization rates at two soil layers and Q10 of topsoil were mainly driven by SOC quality, while it was related to both SOC quality and chemical protection for subsoil, implying that factors controlling the temperature sensitivity of SOC mineralization were depth-specific. Altogether, substrate accessibility as a function of chemical composition and chemical protection would play vital role in determining the feedbacks of the subsoil SOC pool to the future climate warming.

Unlike chemical fertilizer reduction, organic fertilizer substitution increases soil organic carbon stock and soil fertility in wheat fields.

Improvements in farmland soil organic carbon (SOC) stock enhance crop yield and soil fertility while mitigating climate change. Rational fertilization in agricultural production is crucial for safeguarding SOC stock. In this study, field experiments were conducted with different ratios of chemical fertilizer reduction and organic fertilizer substitution for three consecutive years (2018-2020) to explore their effects and interlinkages on SOC fractions, soil properties and SOC stock. The results showed that organic fertilizer substitution increased SOC and its fractions content, SOC stock (by 3.98-12.98% and 7.15-18.13%) and soil fertility index (by 11.76-49.26% and 33.33-91.47%) compared to conventional fertilization in 2019 and 2020, while chemical fertilizer reduction had the opposite effect. Moreover, soil properties (except total nitrogen to total phosphorus ratio, N/P) and SOC fractions significantly affected SOC stock, with SOC fractions contributing more than soil properties. The high sensitivity of microbial biomass carbon (MBC) and dissolved organic carbon (DOC) can indicate changes in soil carbon pool. Structural equation modeling (SEM) revealed that organic fertilizer substitution increased SOC content and stock by increasing SOC fractions [recalcitrant organic carbon (ROC) and labile organic carbon (LOC) fractions] content and soil fertility. Our study revealed the corresponding mechanisms of the two fertilization modes affecting SOC stock changes. The use of organic fertilizer substitution is recommended to increase SOC stocks and soil fertility in wheat fields. © 2023 Society of Chemical Industry.

Straw incorporation into microplastic-contaminated soil can reduce greenhouse gas emissions by enhancing soil enzyme activities and microbial community structure

Microplastic (MP) contamination poses a substantial threat to agroecosystems, disrupting soil properties, nutrient cycles, and microbial communities and ultimately affecting plant growth and ecosystem resilience. The effects of straw addition on the storage of soil organic carbon (SOC) and greenhouse gas emissions have been extensively explored, but these effects have not been examined in the context of MP contamination. To assess the impacts of legume straw and polyethylene microplastics on SOC fractions and carbon dioxide (CO2) and nitrous oxide (N2O) emissions, 7-month soil incubation experiments were performed. The results revealed that the inclusion of legume straw in soil considerably increased microbial SOC compared to the control. However, straw addition to MP-contaminated soil reduced microbial SOC compared to that in soil containing only straw. In contrast, the addition of straw to MP-contaminated soil elevated (+44%) the SOC mineral relative to the sole application of straw. Intriguingly, straw incorporation into MP-contaminated soil reduced microbial biomass carbon and nitrogen relative to soil containing only straw. Straw addition to MP-contaminated soil enhanced the nitrification activity and reduced the relative expression of AOBamoABC gene compared to sole straw-incorporated soil and the control. Greenhouse gas emissions were also modulated; for instance, straw incorporation into MP-contaminated soil reduced CO2 and N2O emissions by −11% and −46%, compared to straw incorporation alone. The urease and phosphatase activities were decreased (−58% and −12%) in the MP-polluted soil with straw incorporation compared with those in the soil in which only straw was applied. However, invertase and catalase activities were upregulated in the straw-incorporated soil contaminated with MPs. Straw addition in the MP-polluted soil considerably enhanced (+2%) the microbial community structure (indicated by PLFA) compared to the sole straw application. These results provide a comprehensive perspective on the role of legume straw incorporation in addressing MP pollution, showcasing its potential for sustainable agricultural practices in the face of evolving environmental challenges.

Mechanism of polyethylene and biodegradable microplastic aging effects on soil organic carbon fractions in different land-use types

Microplastics (MPs) are widely present in terrestrial ecosystems, but knowledge about the aging characteristics of MPs in different land-use types and their impact on soil organic carbon fractions is still limited. Polyethylene (PE) and biodegradable MPs (Poly propylene carbonate and Polybutylene adipate terephthalate synthetic material (PPC + PBAT, Bio)), at 0 %, 0.03 %, and 0.3 % (w/w) dosages, were added to grassland, farmland, and facility soils for eight-week incubation. The aging degree of MPs was explored by quantifying the carbonyl index (CI). Soil organic C fractions such as SOC, particulate organic carbon (POC), mineral-associated organic carbon (MAOC), and microbial-derived C were analyzed. MPs underwent rapid aging after incubation, and the CI value for 0.03 % PE-MPs increased from 0.05 to 0.27 (farmland) and 0.26 (facility) (p < 0.05). The aging degree of 0.03 % and 0.3 % Bio-MPs was most significant in grassland, with CI decreasing by 46.6 % and 69.0 %, respectively. The CI of MPs were negatively correlated with their dosage. The 0.03 % and 0.3 % PE-MPs decreased soil organic carbon (SOC) content by 7.4 % and 8.2 % in grassland, and 3.0 % and 6.0 % in the facility (p < 0.05). POC content of farmland and facility soil was negatively correlated with PE-MPs' CI (p < 0.05). The 0.03 % PE and Bio-MPs decreased fungal necromass C (FNC) by 0.40 and 0.05 g kg−1 in grassland and 0.48 and 0.21 g kg−1 in farmland. Besides, the dosage of MPs regulated FNC content through soil pH, nutrients, and extracellular enzyme activity, either directly or indirectly, ultimately affecting the soil C pool. Therefore, this study demonstrates that MPs strongly affect SOC dynamics by influencing soil microbial enzyme activity and fungal necromass.

Assessment of Lettuce Yield and Soil Organic Carbon Fraction Distribution under Different Types of Organic Matter in South Korea

Assessment of Lettuce Yield and Soil Organic Carbon Fraction Distribution under Different Types of Organic Matter in South Korea

Increasing complexity of agroforestry systems benefits nutrient cycling and mineral-associated organic carbon storage, in south-eastern Brazil

Agroforestry systems are often promoted as solutions to address land degradation and climate change. However, agroforestry is an umbrella term for a large variety of systems and it is not clear how their degree of complexity influences their provision of soil-based ecosystem services, such as soil organic carbon (SOC) storage and nutrient cycling. Furthermore, a knowledge gap remains whether agroforestry systems perform equally well on all soil types. The objectives of this study were 1) to assess the links between agroforestry complexity, nutrient cycling and SOC fractions, and 2) to assess how soil texture influences these relationships in Brazilian agroforestry systems. We sampled 59 agroforestry plots across 30 sites in Sao Paulo state, Brazil, and 8 monocrop sites (6 pastures and 2 crop monocultures). The 38 sites represented a soil textural gradient, ranging from very sandy to very clayey (clay content range 25 – 620 g kg−1). An Agroforestry Complexity Index (ACI) was defined based on tree species richness, stem density and pruning management. Nutrient (N, P, K, Ca, Mg) and C contents were determined in litter and soil (0–30 cm depth) samples, and mineral-associated organic C (MAOC) and particulate organic C (POC) in soil samples were assessed as well. ACI was positively associated with C, N, P, Ca and Mg stocks in litter, and these litter nutrients were in turn positively associated with the corresponding soil nutrient stocks. Associations between soil nutrients and MAOC were stronger on sandy soils than on clayey soils, particularly for P, Ca and Cation Exchange Capacity (CEC). For POC, robust relationships with nutrients were only found on sandy soils. Structural Equation Models indicated causal relationships between agroforestry complexity, P and Ca cycling, and MAOC and POC stocks in topsoils. Our results indicate that nutrients effectively cycle from in situ mulch into plant-available soil pools and highlight the synergies between nutrient cycling and stable C stocks that can be achieved in complex agroforestry systems. These synergies seem to be particularly strong on sandy soils (<15 % clay).

Soil Microbial Community in Relation to Soil Organic Carbon and Labile Soil Organic Carbon Fractions under Detritus Treatments in a Subtropical Karst Region during the Rainy and Dry Seasons

Climate and detritus influence soil organic carbon (SOC) and labile SOC fractions by affecting soil microbial communities. However, it is not clear how, or to what extent, different detritus treatments affect soil microbial communities and SOC content in karst landscapes during different seasons. Plots in a karst landscape were treated with different detritus input regimes (control, no litter, no roots, no litter or roots, and double litter), and samples were collected during the dry and rainy seasons. We used Illumina sequencing of 16S rRNA to examine shifts in the diversity and composition of the associated soil microbial communities. Additionally, labile SOC fractions, including dissolved organic carbon (DOC) and microbial biomass carbon (MBC), along with soil physicochemical properties and C-degrading enzyme activities, were analyzed. The results revealed that the responses of soil properties and labile SOC fractions to detritus treatments were more pronounced during the rainy season than during the dry season, which mainly reflected that the levels of available potassium (AK), DOC, and MBC were significantly increased during the rainy season. Moreover, SOC and total nitrogen (TN) demonstrated significant changes with the double litter (DL) treatment during the rainy season. The responses of soil microbial communities to detritus treatments varied with the season, as reflected primarily in changes in the relative abundance of Ascomycota, unclassified_K_fungi, Proteobacteria, and Actinobacteriota. Climate, detritus treatments, and their interactions had significant effects on the species richness of soil bacterial communities, but did not influence fungal community diversity. Furthermore, structural equation modeling (SEM) revealed that the soil bacterial composition had the largest total effects on SOC, DOC, and MBC. In addition to directly influencing SOC, DOC, and MBC, soil properties (TN, AK, and pH) indirectly affected SOC, DOC, and MBC by altering C-degrading enzyme activity and the microbial community. We conclude that detritus treatments affect the soil microbial community and labile carbon fractions during both the rainy and dry seasons. Relationships among SOC, labile SOC fractions, enzyme activities, microbial communities, and function differed between seasons and among treatment types. This research advances our knowledge of how variation in detritus treatments affects biogeochemical cycling in karst soils during the rainy and dry seasons.

Effect of Short-Term Organic Matter Returns on Soil Organic Carbon Fractions, Phosphorus Fractions and Microbial Community in Cold Region of China

To investigate the effect of different organic matter returns on soil organic carbon (SOC) fractions, phosphorus (P) fractions and microbial communities, a pot experiment was conducted in a cold region of China for three years. There were six treatments in this study, including no rice straw return (S0), rice straw return (SR), decomposed rice straw return (DS), rice-straw-burned return (BS), rice root return (RR) and decomposed cattle manure return (DM). The results indicated that the organic matter returns had no significant effect on the rice yield after three years. The SR, DS and DM treatments significantly increased the content of the soil’s total organic carbon (TOC), light fraction organic carbon (LFOC), particulate organic carbon (POC), dissolved organic carbon (DOC) and microbial biomass carbon (MBC). The BS treatment decreased the soil MBC content. The SR, DS, BS and DM treatments significantly increased the content of the soil’s total P, NaHCO3-P, NaOH-P and residual-P. The proportion of nonlabile P (HCl-P and residual-P) was reduced by the organic matter returns. The SOC fractions were positively correlated to the soil P fractions (except HCl-P). The organic matter returns did not affect the microbial diversity but did change the microbial community composition. The dominant phyla included Proteobacteria, Firmicutes, Chloroflexi, and Bacteroidetes. Compared with the S0 treatment, the organic matter returns increased the relative abundance of Actinobacteria, Anaerolineae and Alphaproteobacteria and decreased the relative abundance of Bacteroidetes, Clostridia and Bacteroidia. The contents of MBC, DOC and NaOH-P were the main factors affecting the microbial community composition, and the soil’s P fractions had a larger influence on the microbial community than the SOC fractions. These results indicated that the incorporation of rice straw, decomposed rice straw and decomposed cattle manure might be an effective practice for maintaining soil fertility in the cold region of China.

Effects of land reclamation on soil organic carbon and its components in reclaimed coal mining subsidence areas

The accumulation of soil organic carbon (SOC) is crucial for the development and ecosystem function restoration of reclaimed mine soils (RMSs). To optimize reclamation management practices, this study aims to explore the factors and underlying mechanisms influencing the recovery of SOC and its components in RMSs from a systemic perspective using complex network theory (CNT). This study focused on coal mining subsidence areas in the eastern mining regions of China, comparing reclaimed cultivated land with surrounding non-subsided cultivated land. Soil samples were collected at depths of 0–20 cm, 20–40 cm, and 40–60 cm, and 25 soil indicators were measured. CNT was applied to explore the intricate relationships between soil indicators and to identify the key factors and underlying mechanisms influencing SOC and its components in RMSs. The results revealed that the compaction-induced soil structural damage during the reclamation process led to a chain reaction, resulting in increased soil bulk density (11.92 % to 15.03 %), finer soil particles (5.00 % to 9.88 % more clay and silt), and enhanced SOC mineralization (SOC decreased by 10.70 % to 15.62 % with a lower C/N ratio by 2.30 % to 28.55 %). Microbial activity also decreased, with a 6.25 % to 13.16 % drop in MBC and a 0.91 % to 27.68 % decrease in enzyme activity. The utilization of active SOC fractions by more adaptable bacterial communities was crucial within this chain reaction process. The intermediate role of soil structure in the RMS ecosystem, particularly in carbon cycling, becomes more prominent. RMSs exhibited heightened sensitivity to soil structure changes, with the response of microorganisms and enzymes to soil structure changes being pivotal. In the carbon cycling process of RMSs, microbial-driven enzyme activity in response to soil structure was more critical during SOC transformation, while the role of physical-chemical protection and microbial inhibition mediated by iron‑aluminum oxides became more pronounced in stabilizing SOC.

The Different Roles of Mineralogy in Soil Organic Carbon Accumulation in Northern and Southern China

The sequestration of soil organic carbon (SOC) through mineral protection is an important approach to mitigating climate change. However, the effect of mineral composition on SOC stability is unclear at regional scales. In this study, we investigated the relationship between mineralogy and SOC in Alfisol and Mollisol from southern and northern regions of China. We analyzed soil at two layers for its SOC fractions, mineralogical characteristics and functional groups. It was found that the majority of SOC was stored as mineral-associated organic C (MAOC), which had higher δ13C values and narrower C/N ratios compared to particulate organic C. In Mollisol, the proportion of MAOC and the abundance of aromatic C were higher than that in Alfisol, while polysaccharide C was lower. Compared to Alfisol, Mollisol was dominated by illite, and had significantly (p &lt; 0.05) lower iron (Fe) and aluminum (Al) sesquioxides contents. The SOC content was positively correlated with illite in Mollisol, and with Fe and Al sesquioxides in Alfisol. The random forest model identified sesquioxides as the most important determinant of SOC accumulation (36%), followed by SOC fractions (18%) and functional groups (18%). In summary, our study suggests that SOC protection through mineralogy depends more on the composition of the host minerals, and not just on the clay content, and aromatic C is also important in the stabilization of SOC.

Reproducibility of Rock-Eval® thermal analysis for soil organic matter characterization

Rock-Eval® (RE) is a thermal analysis technique increasingly used to characterise soil organic matter. To interpret the results, particularly when investigating differences between samples, it is necessary to know the expected ranges of analytical error associated with the RE measurements. Moreover, the RE analyzer is now at its seventh version (RE7) while most literature results were produced using the previous version (RE6). Thus, a characterization of the reproducibility of RE measurements is necessary. We measured the reproducibility of RE measurements using fifteen samples from French croplands and forests that were analysed on five different RE instruments, located in different laboratories and belonging to both generations RE6 and RE7. From each RE analysis, we extracted RE parameters commonly used for soil organic matter characterization and we performed the prediction of the active and stable soil organic carbon fractions using a machine learning model (PartySOC) that uses RE parameters. We obtained a measure of the expected relative errors in RE parameters and PartySOC predictions per instrument, across instruments of the same generation and across generations. We found that the parameters total organic carbon (TOC), mineral carbon (MinC) and R-index are well reproducible, even across the RE6 and RE7 generations. Instead, the hydrogen index (HI) and oxygen index (OI) are more sensitive to signal variations, even within the same generation, especially when TOC is low. The PartySOC predictions were well reproducible across RE6 instruments but not across RE generations. In the future, the results of this study will help discriminate relevant differences between soil samples characterised using RE thermal analysis.

Natural and regenerated saltmarshes exhibit different bulk soil and aggregate-associated organic and inorganic carbon contents but similar total carbon contents

Saltmarshes are considered to be one of the planet's most efficient carbon sinks. The continued loss of saltmarshes and induced ecological consequences promoted their restoration worldwide. Previous efforts aimed to evaluate the success of restoration in terms of organic carbon accumulation, but inorganic carbon and carbon contents within soil aggregates, which are essential for making a comprehensive assessment of the carbon sink function, were rarely studied. To fill this gap, a range of metrics including bulk and aggregate-associated soil organic and inorganic carbon contents together with the soil's physical, chemical and microbiological parameters were measured to compare natural and a 15-year restoration effort in saltmarsh habitats within the Yellow River Delta region in eastern China. The results showed that regenerated saltmarsh exhibited significantly higher soil organic carbon (SOC) contents but significantly lower soil inorganic carbon contents, resulting in no notable change in total carbon contents between the regenerated and natural saltmarshes. SOC contents within the silt and clay fractions and their contribution to the bulk SOC contents were significantly lower in the regenerated saltmarsh than those in the natural ones (P < 0.05). In regenerated saltmarsh, significantly lower soil microbial biomass and distinct microbial community composition with reduced Gram-negative to Gram-positive bacteria ratios were observed compared to natural saltmarsh. These findings indicate the stability of SOC fraction and soil microbe-mediated carbon biogeochemical processes differed between naturally occurring and artificially regenerated saltmarshes. As interest in blue carbon programs gains global attention, further research on the generation and transformation processes of different carbon fractions during restoration are needed, which can be conducive to elucidating more details in coastal carbon cycling processes.

Adaptive evaluation for agricultural sustainability of different fertilizer management options for a green manure-maize rotation system: Impacts on crop yield, soil biochemical properties and organic carbon fractions

Green manure planting can reduce the intensity of soil use, while improving farmland productivity in double-cropping systems. However, only few studies have focused on the impacts of green manure application under different fertilization management options on succeeding crop yield and soil organic carbon (SOC) process. A three-year field experiment was conducted with a winter smooth vetch-summer maize cropping system to evaluate the effects of green manure with different chemical fertilizers on soil physiochemical properties, SOC fraction, enzyme activities and maize yield. Total eight treatments were compared including different combinations of green manure and chemical fertilizers (i.e., nitrogen and phosphorus fertilizers) in the smooth vetch phase and maize phase. The results showed that compared to the control, green manure incorporation increased the soil moisture, total nitrogen, total phosphorus, basal respiration, SOC and its labile fractions, and enzyme activities, especially for the treatments of green manure with fertilization. However, the soil pH and bulk density decreased due to green manure application. Maize yield increased 34 %–53 % after green manure application, and was found to be significantly and positively correlated with soil carbon process (P < 0.05). Moreover, SOC and its labile fractions, and total nitrogen were observed as the main drivers of the maize yield. Variation partition analysis demonstrated that soil biochemical properties and their interaction with green manure by fertilization caused variations in SOC fractions. Further, structural equation models indicated that both balanced fertilization practices had positive effects on maize yield and soil carbon process via changes in SOC fractions and C cycling-related enzyme activities, respectively. In addition, the amount balance of chemical fertilizer positively impacted the soil carbon process by regulating SOC fractions through enzyme activities. These findings provide important guidance for applying optimal fertilization management in the green manure phase to improve succeeding crop yield and soil quality as well as to mitigate the adverse impacts of chemical fertilizers. The study will be equally illuminating for other green manure-crop rotation systems.

Precipitation change affects forest soil carbon inputs and pools: A global meta-analysis

The impacts of precipitation change on forest carbon (C) storage will have global consequences, as forests play a major role in sequestering anthropogenic CO2. Although forest soils are one of the largest terrestrial C pools, there is great uncertainty around the response of forest soil organic carbon (SOC) to precipitation change, which limits our ability to predict future forest C storage. To address this, we conducted a meta-analysis to determine the effect of drought and irrigation experiments on SOC pools, plant C inputs and the soil environment based on 161 studies across 139 forest sites worldwide. Overall, forest SOC content was not affected by precipitation change, but both drought and irrigation altered plant C inputs and soil properties associated with SOC formation and storage. Drought may enhance SOC stability by altering soil aggregate fractions, but the effect of irrigation on SOC fractions remains unexplored. The apparent insensitivity of SOC to precipitation change can be explained by the short duration of most experiments and by biome-specific responses of C inputs and pools to drought or irrigation. Importantly, we demonstrate that SOC content is more likely to decline under irrigation at drier temperate sites, but that dry forests are currently underrepresented across experimental studies. Thus, our meta-analysis advances research into the impacts of precipitation change in forests by revealing important differences among forest biomes, which are likely linked to plant adaptation to extant conditions. We further demonstrate important knowledge gaps around how precipitation change will affect SOC stability, as too few studies currently consider distinct soil C pools. To accurately predict future SOC storage in forests, there is an urgent need for coordinated studies of different soil C pools and fractions across existing sites, as well as new experiments in underrepresented forest types.

Soil conditioners promote the formation of Fe-bound organic carbon and its stability

The close association of soil organic carbon (SOC) with Fe oxides is an important stabilization mechanism for soil organic matter (SOM) against biodegradation. Soil conditioners are of great importance in improving soil quality and soil health. Yet it remains unclear how different conditioners would affect the fractionation of SOC, particularly the Fe-bound organic carbon (Fe-OC). Field-based experiments were conducted in farmland to explore the fractionation of organic carbon (OC) and Fe oxides under the effects of three different soil conditioners (mineral, organic, and microbial conditioners). The results showed that all soil conditioners increased the total OC and Fe-OC contents, with the contribution of Fe-OC to total OC increasing from 1.57% to 2.99%. The low OC/Fe molar ratio indicated that surface adsorption played a crucial role in soil Fe-OC accumulation. Nuclear magnetic resonance (NMR) results suggested that soil conditioner altered the composition of SOM, accelerating O-alkyl C degradation and increasing recalcitrant alkyl C and aromatic C sequestration. Scanning electron microscopy with energy dispersive spectroscopy (SEM-EDS) analysis indicated that all conditioners promoted the association of OC and Fe oxides. Furthermore, comprehensive analysis of 13C isotope and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy showed that the mineral conditioner enhanced the association of microbial-derived OC and Fe oxides, whereas the organic conditioner increased the association of plant-derived OC with Fe oxides. These findings provide important insights into the potential mechanisms through which soil conditioners regulate the stability of OC and guide agricultural management.

Implications of surfactant application on soil hydrology, macronutrients, and organic carbon fractions: An integrative field study

Implications of surfactant application on soil hydrology, macronutrients, and organic carbon fractions: An integrative field study

Straw and straw biochar differently affect fractions of soil organic carbon and microorganisms in farmland soil under different water regimes

Incorporating amendments can change soil organic carbon (SOC) components and biological properties, but the comprehensive utilization of straw and straw biochar in paddy soil remains poorly understood. We studied the changes in the pH, SOC content, labile organic C fractions, and soil microbial communities under alternating wetting and drying (AWD) and continuous flooding (CF). The results showed that the addition of straw and straw biochar increased SOC content, and the relative changes in microbial biomass carbon (MBC), dissolved organic carbon (DOC), and easily oxidizable carbon (EOC) were more sensitive than those in SOC, but soil pH was higher under AWD management than under CF management. Proteobacteria, Bacteroidetes, and Acidobacteria were the three most abundant bacterial phyla, and the three major abundant fungal phyla were Phragmoplastophyta, norank_Eukaryta, and Apicomplexa. A redundancy discriminant analysis (RDA) found that both straw and straw biochar were conducive to bacterial growth, and their combination was more beneficial for fungal growth and development. Aggregated boosted tree (ABT) and Structural equation modeling (SEM) showed that the soil MBC and EOC were the most important factors determining bacterial abundance and diversity, while pH, EOC, and MBC were the main factors influencing the abundance and diversity of fungi. This study explored the changes in SOC fractions and bacterial and fungal communities and provided a theoretical basis for an in-depth understanding of the influence of labile organic C pools on bacterial and fungal communities.

Distribution characteristics of soil organic carbon fractions in paddy profiles with 40 years of fertilization under two groundwater levels

Distribution characteristics of soil organic carbon fractions in paddy profiles with 40 years of fertilization under two groundwater levels

Comparison of organic carbon properties in extracted soil solutions obtained underneath Cryptomeria japonica and Quercus acutissima and its implication on stream dissolved organic carbon

Dissolved organic carbon (DOC) in soils is released into streams, working as a main component of the carbon cycle. DOC in terrestrial and aquatic ecosystems participates in many biogeochemical reactions and processes such as heterotrophic respiration, sorption of metals, and transport of pollutants. In order to understand the connectivity of organic carbon among soil, soil water, and forest streams, we investigated the concentrations and dual carbon isotope ratios (δ13C and Δ14C) of soil organic carbon (SOC) and water-extractable organic carbon (WEOC) obtained from soils beneath two tree species stands, and compared these with stream Δ14C-DOC of the forest watershed. Soil samples were collected at different depths (0–10, 10–30, and 30–50 cm) beneath Japanese cedar (Cryptomeria japonica) and sawtooth oak (Quercus acutissima). Although the SOC concentration was not significantly different between the two tree species, the WEOC concentration ([WEOC]) of soil at 0–10 cm depth under Cryptomeria japonica was higher than that of Quercus acutissima, in general. The δ13C-SOC and δ13C-WEOC increased, while the Δ14C-SOC and Δ14C-WEOC decreased with increasing soil depth. The Δ14C-WEOC was higher than the Δ14C-SOC, indicating that WEOC could be primarily derived from the young, hydrophilic, and exchangeable fraction of SOC, rather than from SOC strongly bonded to mineral soils. However, the stream Δ14C-DOC was lower than Δ14C-WEOC in general, except during summer storms. The 14C-depleted DOC released from deep soils or groundwater might lower Δ14C-DOC of a stream, suggesting that relatively old DOC could be released into streams during baseflow. This is contrary to the results of previous studies that have reported positive stream Δ14C-DOC from temperate forests. The discrepancy warrants future research on forest stream Δ14C-DOC across entire seasons, particularly under Asian monsoon climates.

Shallow-incorporated straw returning further improves rainfed maize productivity, profitability and soil carbon turnover on the basis of plastic film mulching

Ridge and furrow plastic film mulching (RFM) has improved net primary productivity (NPP) to a high level in semi-arid rainfed agricultural regions. Yet, it is unclear whether there is still a room for further improvement on NPP while stabilizing soil organic carbon through returning previous maize stover pieces. To address this issue, maize stubbles were smashed into pieces and in situ ploughed into 30 cm topsoil at a semi-arid site in northwest China from 2016 to 2017. This was done using the Pioneer 335 maize variety with three treatments (CK, ridge and furrow without mulching; RFM, ridge and furrow with plastic mulching; RFML, 3.49 t ha−1 of maize stover returning in RFM; RFMH, 5.24 t ha−1 of returning in RFM). The results indicated that maize stover returning exhibited similar trend as RFM at early cool seedling stage, raising the topsoil temperature. However, in warm and dry silking stage (2017), both stover returning rates reduced soil temperatures by 1.33 and 0.8 ◦C relative to RFM and CK (P < 0.05), respectively. The soil water storage increased significantly in RFMH by 17.3% and RFML by 28.5% relative to that of RFM, in a warm and dry growing season (2017). Critically, stover returning increased (P < 0.05) soil organic carbon and light fraction organic carbon turnover across the two growing seasons. By improving hydrothermal conditions, stover returning in RFM increased biomass accumulation and grain yield (P < 0.05), leading to higher (P < 0.05) net economic benefit, with greater above-ground biomass NPP (74.5–93.2%) and below-ground biomass NPP (88.5–89.4%), compared with CK. In conclusion, maize stover returning of stover pieces into topsoil might be a promising solution to enhance carbon turnover for higher net primary productivity on the basis of plastic film mulching in semiarid rainfed region.