Excessive Soil Research Articles

The E3 ubiquitin ligase COP1 regulates salt tolerance via GIGANTEA degradation in roots.

Excess soil salinity significantly impairs plant growth and development. Our previous reports demonstrated that the core circadian clock oscillator GIGANTEA (GI) negatively regulates salt stress tolerance by sequestering the SALT OVERLY SENSITIVE (SOS) 2 kinase, an essential component of the SOS pathway. Salt stress induces calcium-dependent cytoplasmic GI degradation, resulting in activation of the SOS pathway; however, the precise molecular mechanism governing GI degradation during salt stress remains enigmatic. Here, we demonstrate that salt-induced calcium signals promote the cytoplasmic partitioning of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1), leading to the 26S proteasome-dependent degradation of GI exclusively in the roots. Salt stress-induced calcium signals accelerate the cytoplasmic localization of COP1 in the root cells, which targets GI for 26S proteasomal degradation. Align with this, the interaction between COP1 and GI is only observed in the roots, not the shoots, under salt-stress conditions. Notably, the gi-201 cop1-4 double mutant shows an enhanced tolerance to salt stress similar to gi-201, indicating that GI is epistatic to COP1 under salt-stress conditions. Taken together, our study provides critical insights into the molecular mechanisms governing the COP1-mediated proteasomal degradation of GI for salt stress tolerance, raising new possibilities for developing salt-tolerant crops.

Evaluating installation of suction caisson in sand via accounting for soil seepage-deformation coupled process

Suction-assisted installation is one of the special features that distinguishes the suction caisson from other offshore foundations. Suction control during installation can be challenging, as it involves highly coupled processes between seepage flow and seabed soil deformations, including excessive soil deformation and plug formation under strong seepage actions that lead to early termination of the installation, as well as a seepage flow-induced reduction in caisson penetration resistance. However, existing methods for evaluating suction caisson installation are primarily based on pure seepage analysis while disregarding the above-coupled processes. This paper proposes an approach to evaluate suction caisson installation in sand by explicitly accounting for seepage-deformation coupling. For this purpose, pore pressure-deformation coupled finite element analyses are performed to investigate soil failure mechanism and evolving caisson penetration resistance subjected to suction. These analyses result in novel rules for assessing critical suction upon the rapid growth of soil deformation and describing the degradation of caisson penetration resistance. The latter two propositions lead to a new calculation method for determining the required suction for caisson installation. The performance of the calculation method is evaluated against field tests and centrifuge tests to illustrate the usefulness of considering coupled soil deformation-seepage process in designing caisson installation.

Precise soil coverage in potato planting through plastic film using real-time image recognition with YOLOv4-tiny

Planting potatoes through plastic film with incomplete or excessive soil coverage over seed holes significantly impairs yield. Existing covering methods rely solely on mechanical transmissions, leading to bulky and inconsistent soil coverage of the seed holes. This paper reports an innovative method using a precise soil covering device based on the YOLOv4-tiny real-time object detection system to accurately identify potato plastic film holes and cover them with soil. The system adopts a lightweight and high-precision detection scheme, balancing increased network depth with reduced computation. It can identify holes in the plastic film in real-time and with high accuracy. To verify the effectiveness of YOLOv4-tiny real-time object detection system, a precise soil covering device based on this detection system has been designed and applied to a double crank multi-rod hill-drop planter. Field tests revealed that the system's average accuracy rate for detecting holes is approximately 98%, with an average processing time of 15.15 ms per frame. This fast and accurate performance, combined with the device's robust real-time operation and anti-interference capabilities during soil covering, effectively reduce the problems of soil cover omission and repeated covering caused by existing mechanical transmission methods. The findings reported in this paper are valuable for the development of autonomous potato plastic film precise soil covering devices for commercial use.

Participatory Demonstration of Durum Wheat Production Blending Vertisols Management Technologies in Lume District, East Shewa, Ethiopia

Durum wheat production on Vertisols is constrained by waterlogging, where excessive soil moisture limits nutrient availability, resulting in lower yields and higher input demand. Aybar broad bed maker (BBM) was recommended to make broad beds and furrows (BBF) and drain the excess water although it is not widely adopted by farmers. Therefore, this demonstration activity was conducted to create awareness about the importance of BBF along with the recommended fertilizer rates in Lume district during the 2021 and 2022 main cropping seasons. Nine target farmers were purposefully selected based on the ground that their farms are waterlogged Vertisols. Aybar BBM to make the BBF with an 80 cm bed and 20 cm furrow width, while recommended fertilizers at a rate of 92 kg ha<sup>-1</sup> nitrogen (N) and 10 kg ha<sup>-1 </sup>phosphorous (P) were used. The yield data and farmers’ perception were analyzed using t-test, descriptive statistics, and narration, while partial budget analysis was conducted to examine the economic feasibility of the technologies. Results showed that growing durum wheat on BBF by applying the recommended N and P rates resulted in significantly higher grain yield (3474 kg ha<sup>-1</sup>) <i>with a 65.4% yield advantage </i>compared to ridge and furrow (RF) (2100 kg ha<sup>-1</sup>) with the blanket fertilizer rates. The partial budget analysis revealed that land preparation using Aybar BBM, alongside the recommended fertilizer rates, brought a net benefit of 48,563 ETB ha<sup>-1</sup> over the farmers' practice. Likewise, the marginal rate of return for changing from the local plow to Aybar BBM is 901.7%. The farmers also witnessed that the Aybar BBM even takes less plowing time and energy, and they want to continue to use it. <i>In light of these results, the study concludes that the use of Aybar BBM and recommended N and P rates, as a production package on waterlogged Vertisols, is profitable for farmers and recommends promoting and scaling up these technologies in the study area and other waterlogged Vertisols regions of the country.</i>

Responses of crops for diverse soil phosphorus conditions and potential crops for phytoextraction of enriched soil phosphorus

Phytoextraction is an attractive method to extract excess soil phosphorus (P) builds up from the soils. The crops potential to extract an enriched P in soil is still lacking. Therefore, four crops were grown in three different soils with variable P levels (0 to 200 mg P/kg soil) and screened for P acquisition potentials. Total P accumulation in roots and shoot was high with enriched P levels in all soils, for example in soil A at 200 mg P/kg soil, the values were 0.44% for cucumber (Cucumis sativus), 0.40% for sunflower (Helianthus annus), and ∼0.30% for cabbage (Brassica oleracea) and 0.20% for Indian mustard (Brassica juncea). The cucumber and sunflower showed a greater biomass production (>6.0 g/pot) with enriched P levels and exhibited more prominent root characteristics, suggests more favorable for P acquisition from the P enriched soils. More prominent enhanced phosphomonoesterase and phytase activities in these crops possibly contributing to their P accumulation ability under high soil P conditions. This study shows that, cucumber and sunflower can generate high dry matter content and can accumulate greater quantity of P from the soils through different mechanisms and they can be potential candidates for phytoextraction of P from the soil P enriched areas.

Structural performance of fibre reinforced recycled aggregate concrete road kerb sections under monotonic and cyclic loading

In recent years, there has been a notable emphasis on waste reduction and the adoption of recycled materials within the construction industry to reduce the industry’s overall carbon footprint. This study investigates the structural performances of concrete kerb sections prepared with five different concrete mixes containing recycled concrete aggregate, recycled tyre-derived aggregates and recycled polypropylene fibres. Kerb sections were cast at a road site in a suburb of Adelaide, Australia. After the concrete hardened, sections were cut and brought to the laboratory. A large number of monotonic and cyclic load tests were conducted on the kerb sections. The load-carrying capacity, bending moment capacity, cyclic fatigue capacity, durability properties along with deformation tolerance were evaluated. Kerb sections made with concrete containing recycled aggregate and polypropylene fibre could sustain nearly 2000 cycles of loading. Kerb sections prepared with natural aggregate concrete performed comparatively better. The addition of polypropylene fibre significantly improved the post-cracking behaviour of kerb sections and can delay crack propagation and other distress when subjected to cyclic loadings such as excessive soil movement, e.g., in areas with expansive soils or prone to tree root migration. Long-term observation may be required to confirm the mechanical and durability performance improvement in real field conditions.

Spatially refined salinity hazard analysis in gypsum‐affected irrigated soils

AbstractThe global extent of salt‐affected agricultural land, 20% of which is deemed gypsiferous, results in billions of dollars of annual economic loss, a serious problem deserving of attention. However, the analysis of gypsiferous saline soils, such as in the irrigated Lower Arkansas River Valley (LARV) of Colorado, can result in an inflated estimation of soil salinity when using the traditional soil saturated paste extract electrical conductivity (ECe), leading to inaccurate crop yield loss predictions and misguided decisions for remediation. Sparingly soluble gypsum (CaSO4 2H2O) in these soils dissolves more readily during laboratory preparation of saturated paste extracts because of excess soil water dilution coupled with sample disturbance. We present a pragmatic linear‐regression approach to correct for this phenomenon, calibrated using two adapted methods for correcting ECe on an individual sample basis. The novel approach used electrical conductivity of pore water samples from saline fields to evaluate the accuracy of the correction methods. The approach was applied on soil samples from two surface‐irrigated, saline fields in the LARV, which were mapped using electromagnetic induction data and analysis of covariance linear regression, calibrated for ECe and ECe corrected for excess gypsum dissoultion (ECeg). Average ECeg values are as much as 26% lower than uncorrected ECe in gypsum‐biased portions of the fields. Estimation of corn salinity hazard in these gypsum‐affected areas using ECeg in lieu of ECe in a traditional yield response function generated mean relative yield values that are higher by up to 13 percentage points. We discuss lessons learned and suggest enhancements to the techniques.

Germination test, seedling growth, and physiochemical traits are used to screen okra varieties for salt tolerance

Excess soil salinity is a major stress factor that inhibits plant growth, development, and production. Among the growth stages, seed germination is particularly susceptible to salt stress. Okra, a nutraceutical vegetable, has a low germination percentage. Literature has revealed genetic diversity in okra, which can be studied to develop salt-tolerant varieties. This study examined the salt tolerance of 13 okra varieties using germination tests and then tested five varieties in pot experiments with different NaCl levels (75, 100, and 125 mM NaCl). Results showed that salt levels affected all varieties, with differential variations in stress response. Salt stress reduced agronomic, and physiochemical traits in the studied varieties. In variety “MALAV-27”, the highest salt concentration significantly reduced the shoot length (68.12 %), root length (65.11 %), shoot fresh weight (78.73 %), root fresh weight (68.32 %), shoot dry weight (75.60 %), and root dry weight (75.81 %), along with different physiochemical traits. Variety “NAYAB-F1” performed the best, and maintained the highest shoot length (57.12 %), root length (58.72 %), shoot fresh weight (68.26 %), and root fresh weight (58.34 %), shoot dry weight (69.23 %), root dry weight (62.50 %), and numerous physiochemical traits such as sugar (0.74 μg/g), proline (0.51 μmol/g), and chlorophyll ‘a’ (7.97 mg/g), chlorophyll ‘b’ (9.56 mg/g). The study recommended ‘NAYAB-F1′, ‘Arka anamika’, and ‘Shehzadi’ as salt-tolerant varieties suitable for selection in salt-tolerant okra breeding programs.

Water Stress and Black Cutworm Feeding Modulate Plant Response in Maize Colonized by Metarhizium robertsii.

Plants face many environmental challenges and have evolved different strategies to defend against stress. One strategy is the establishment of mutualistic associations with endophytic microorganisms which contribute to plant defense and promote plant growth. The fungal entomopathogen Metarhizium robertsii is also an endophyte that can provide plant-protective and growth-promoting benefits to the host plant. We conducted a greenhouse experiment in which we imposed stress from deficit and excess soil moisture and feeding by larval black cutworm (BCW), Agrotis ipsilon, to maize plants that were either inoculated or not inoculated with M. robertsii (Mr). We evaluated plant growth and defense indicators to determine the effects of the interaction between Mr, maize, BCW feeding, and water stress. There was a significant effect of water treatment, but no effect of Mr treatment, on plant chlorophyl, height, and dry biomass. There was no effect of water or Mr treatment on damage caused by BCW feeding. There was a significant effect of water treatment, but not Mr treatment, on the expression of bx7 and rip2 genes and on foliar content of abscisic acid (ABA), 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), and gibberellin 19 (GA19), whereas GA53 was modulated by Mr treatment. Foliar content of GA19 and cis-Zeatin (cZ) was modulated by BCW feeding. In a redundancy analysis, plant phenology, plant nutrient content, and foliar DIMBOA and ABA content were most closely associated with water treatments. This study contributes toward understanding the sophisticated stress response signaling and endophytic mutualisms in crops.

Experimental and numerical modeling on vacuum consolidation behavior of staged-filled soil slurry with prefabricated horizontal drain and flocculant

The vacuum-assisted prefabricated horizontal drain offers a promising method for strengthening soil slurry, allowing simultaneous filling and vacuum-dewatering via staged construction. However, there is limited research on the unique characteristics of staged filling. This study aims to investigate the vacuum consolidation process of staged-filled soil slurry through laboratory model tests and numerical simulations, also assessing the impact of anionic polyacrylamide. Comparative analyses are conducted between vacuum consolidation with and without anionic polyacrylamide, as well as self-weight consolidation without anionic polyacrylamide. Results reveal contour lines of excess pore pressure, water content, and soil strength forming an ellipse around the prefabricated horizontal drain board. During the consolidation process, a higher degree of consolidation, lower water content, and higher soil strength were observed closer to the prefabricated horizontal drain board. After treatment, the uppermost filling layer exhibits an average water content that was approximately 40% higher than the lower filling layer, and its average strength was about 60% lower. This discrepancy is primarily due to the absence of sealing on the top surface and the relatively short vacuum consolidation time caused by staged filling. The introduction of anionic polyacrylamide-induced flocculation significantly improves the initial consolidation rate but minimally affects the dewatering capacity of vacuum preloading. Using flocculant can enhance both the staged filling rate and soil strength (by 1–2 times). Additionally, employing a staggered arrangement between different prefabricated horizontal drain layers is advisable to prevent top-down penetration in areas with low soil strength.

Holistic evaluation of inlet protection devices for sediment control on construction sites

To address the deleterious impacts of excess soil erosion from the construction sites, the United States Clean Water Act requires that erosion and sediment control measures (ESCs) be implemented on construction projects disturbing more than 0.4 ha. Inlet protection devices (IPDs) are a common ESC utilized on construction projects to reduce the amount of sediment entering storm sewers. In Ohio, regulatory agencies use approved, non-proprietary IPDs made from commonly available materials (e.g., silt fence, geotextile, lumber, and aggregate) to mitigate sediment on construction projects; however, these IPDs often rely on extended ponding to remove sediment and require frequent maintenance making these unsuitable for road construction projects. Commercially manufactured (i.e., proprietary) IPDs which rely on filtration to quickly dewater following rainfall may prove more practical for road construction projects. However, little research which quantitatively compares the holistic performance of these two types of IPDs in field settings has been performed to date. To address this knowledge gap, the performance of 24 proprietary IPDs was evaluated at field-scale using simulated construction site runoff and compared to three non-proprietary IPDs currently approved for use in Ohio. Bypass flows, which typically occurred due to poor IPD fit to standard drainage inlets used in Ohio transportation settings, significantly increased effluent total suspended solids (TSS) and turbidity compared to tests of IPDs where bypass did not occur. Overflow, or intentional bypass around primary IPD flow pathways during high flows, did not significantly impact effluent water quality. Despite differences in treatment mechanisms (i.e., sedimentation versus filtration), the water quality performance of non-proprietary and proprietary IPDs were not statistically different, indicating comparable sediment removal was provided by both categories. Findings from this research can provide design engineers and state regulatory agencies the necessary tools to evaluate IPD performance in road construction settings and, ultimately, alleviate the impact of excess sediment discharged from construction sites.

From Fields to Families: Understanding the Health Impacts of Excessive Soil Fertilization

Background: Fertilizers play a crucial role in modern agriculture, significantly boosting crop yields and global food production. However, their widespread use has unintended consequences that raise significant environmental and health concerns. Nitrogen-based, phosphorus-based, and potassium-based fertilizers contribute to nutrient replenishment in soils but can lead to soil and water contamination, posing risks to ecosystems and human health. Objective: This narrative review aims to comprehensively examine the health and environmental impacts of excessive soil fertilization, with a focus on understanding the mechanisms by which fertilizers affect soil and water quality and the subsequent implications for human health. Methods: An extensive literature search was conducted across multiple electronic databases, including PubMed, Web of Science, Scopus, and Google Scholar. Articles published between 2000 and 2023 were selected based on predefined inclusion criteria, focusing on studies that provided empirical data or substantial theoretical insights into the environmental and health impacts of fertilizer use. A qualitative analysis was performed to synthesize findings and identify common themes. Results: The review highlighted that nutrient runoff from excessive fertilizer use leads to significant water quality degradation, with nitrate concentrations in some regions exceeding recommended safety limits by 30-50%. Prolonged exposure to contaminated water is linked to various health issues, including a 20% increased risk of methemoglobinemia in infants and a 15-25% higher risk of gastric and colorectal cancers in adults. Additionally, heavy metal accumulation in crops due to fertilization practices raises concerns, with cadmium levels in some crops surpassing safe consumption limits by 40-60%. Conclusion: While fertilizers are essential for enhancing agricultural productivity, their excessive use poses significant environmental and health risks. A balanced approach integrating sustainable agricultural practices, effective water management, and comprehensive regulatory frameworks is imperative to mitigate these adverse effects. Continued research and collaboration among scientists, policymakers, and the agricultural community are necessary to promote a sustainable and health-conscious approach to fertilizer use.

Multicontamination Toxicity Evaluation in the Model Plant Lactuca sativa L.

Many contaminated soils contain several toxic elements (TEs) in elevated contents, and plant-TE interactions can differ from single TE contamination. Therefore, this study investigated the impact of combined contamination (As, Cd, Pb, Zn) on the physiological and metabolic processes of lettuce. After 45 days of exposure, TE excess in soil resulted in the inhibition of root and leaf biomass by 40 and 48%, respectively. Oxidative stress by TE accumulation was indicated by markers-malondialdehyde and 5-methylcytosine-and visible symptoms of toxicity (leaf chlorosis, root browning) and morpho-anatomical changes, which were related to the change in water regime (water potential decrease). An analysis of free amino acids (AAs) indicated that TEs disturbed N and C metabolism, especially in leaves, increasing the total content of free AAs and their families. Stress-induced senescence by TEs suggested changes in gas exchange parameters (increase in transpiration rate, stomatal conductance, and intercellular CO2 concentration), photosynthetic pigments (decrease in chlorophylls and carotenoids), a decrease in water use efficiency, and the maximum quantum yield of photosystem II. These results confirmed that the toxicity of combined contamination significantly affected the processes of lettuce by damaging the antioxidant system and expressing higher leaf sensitivity to TE multicontamination.

The effect of sand-crumb rubber mixture treatment on the seismic response of a low-rise building located on liquefiable soil

One of the most effective measures to reduce earthquake risks in high-seismic areas is to reduce the input forces caused by earthquakes to the structure. The properties of rubber materials in geotechnical projects are energy absorption, dynamic deformation change as well as damping of latent energy in loads, high shear modulus and damping ratio, easy access and their economic nature. In this research, with finite difference modeling, the effect of using sand- crumb rubber layer on the seismic behavior of the model has been obtained. In the following, by changing the thickness and depth of the sand-crumb rubber layer, the seismic behavior such as floor displacement and internal forces have been investigated. This research numerically models liquefaction (UBCsand with FLAC) and predicts the effects of the sand-crumb rubber layer of different thicknesses (as % weight of the mixture) under the foundation and evaluates the structure's damage and settlement interacting with the soil by validating the numerical model with the laboratory model and calibrating it with different ratios. The results show that liquefaction, pore water pressure excess ratio and soil settlement will be significantly reduced with the presence of sand-crumb rubber layer, and also the base shear has shown an increase, but the structural damage is reduced.Graphical

Study of frictional wear and desorption of nanoparticle‐rubber blend modified UHMWPE composites

AbstractTo address the issues of abrasive wear, impact wear, and soil adhesion that can lead to wear failure and excessive operational resistance in agricultural soil touching parts during farming. This study focuses on UHMWPE composites, modified by filling Nano‐SiC as a hard phase filler and XNBR as an elastic filler. These fillers were dispersed into the UHMWPE matrix through melt blending technology to create a high‐performance composite material for the surface protective material of soil touching parts. The study discovered that by adding hard and elastic fillers to the UHMWPE matrix, the density, hardness, flexural modulus and thermal stability of the materials were all increased. Specifically, the density increased by 5.67% and the flexural modulus increased by 107%. In the block on ring wear test the volumetric wear rate decreased by 98%, while in the mortar wear test the mass wear rate decreased by 64.64%. Additionally, the contact angle on the surface of the specimen after mortar erosion and wear increased to 102.76°, 18% higher than that of pure UHMWPE. These results demonstrate that the modified fillers can improve the abrasion and plastic deformation resistance and hydrophobic desorption of UHMWPE. UHMWPE composite material, serving as the surface protection for soil touching parts, resolves the issues of abrasion of soil abrasive particles and excessive soil adhesion resistance on these parts. This significantly prolongs the service life of soil touching parts of agricultural machinery and improves the operational efficiency and economy.Highlights Research on nanoparticles and elastomer‐modified composite materials. Research has shown the enhancement of UHMWPE composite materials by Nano‐SiC. XNBR improves the plastic deformation resistance of UHMWPE composites. Effects of Nano‐SiC and XNBR on the desorption behavior of composite materials. UHMWPE composites reduce soil contact wear and soil adhesion.

Analysis of ginseng rusty root symptoms transcriptome and its pathogenesis directed by reactive oxygen species theory.

Ginseng rusty root symptoms (GRS) is a primary disease of ginseng, which seriously decreases the yield and quality of ginseng and causes enormous losses to ginseng production. GRS prevention and control is still challenging due to its unclear etiology. In this study, the phloem tissue of healthy Panax ginseng (AG), the nonred tissue of the phloem epidermis around the lesion (BG), and the red lesion site tissue of GRS (CG) were extracted for mRNA transcriptomic analysis; 35,958 differentially expressed genes (DEGs) were identified and were associated with multiple stress resistance pathways, reactive oxygen species (ROS), and iron ion binding. Further study showed that the contents of O2 •-, H2O2, and malondialdehyde (MDA) were significantly increased in BG and CG tissues. Under anaerobic conditions caused by excessive soil moisture, the overproduction of ROS destroys cell membranes, simultaneously converting Fe2+ to Fe3+ and depositing it in the cell wall, which results in GRS, as evidenced by the success of the GRS induction test.

Study on Effect of Bio Control Agent Trichoderma viride and Neem Cake on Rhizome Rot of Turmeric

Turmeric (Curcuma longa) is one of the major spice crops in Andhra Pradesh, which is severely affected by rhizome rot (soil borne disease) and leaf spots (foliar diseases). There was drastic reduction in yield, which is majorly affected by soil borne fungus. So, the present study mainly intends to evaluate the performance of Trichoderma viride along with neem cake against rhizome rot disease. The experiment was conducted in three successive years at 10 villages and 30 locations of Krishna district as a Front-Line Demonstration of Krishi Vigyan Kendra, Ghantasala, Acarya N G Ranga Agricultural University. The treatment (TO1) consists of seed treatment, ridge sowing, excess water drainage and soil application of T viride culture 2 kg multiplied in 90 kg Farm Yard Manure and 10 kg neem cake at the last ploughing time and use of neem cake 300 kg/acre at 60 and 120 DAP at plant base. Whereas, the farmer’s practice (TO2) was drenching of copper oxy chloride @ 3g /l of water only to control rhizome rot. In comparing the average data of both the treatments for three consecutive years, TO1 recorded highest yield of 49.12 t/ha of fresh turmeric with a yield improvement of 32.43 % over control. In case of rhizome rot incidence, the average percent incidence for the three consecutive years was reported as 3.37 % in demo and 10.72% in control. In case of net returns, Rs. 2,08,743/- per ha was obtained with a B:C ratio of 1.99:1 in demo and Rs. 93,428.17/- of per ha with 1.43:1 B:C ratio in control. It can be concluded that apart from using only chemicals, integrated management practices fetch better yield and returns too.

Performance of surface, subsurface and trench‐type drainage systems in paddy fields for non‐rice farming

AbstractEffective drainage is a crucial factor in paddy fields, especially in regions with waterlogging or heavy clay soils. Identifying an effective drainage system is essential for the successful removal of excess soil water from paddy fields to prepare them for subsequent crops. This study aimed to evaluate three different drainage systems, shallow surface drainage (shallow ditch), conventional subsurface pipe drainage and trench‐type subsurface drainage (French drain), in paddy fields in terms of drainage water volume, water table depth, drainage intensity, soil moisture and cracks. Experiments were carried out in a physical model capable of simulation with a 7.5 m drain spacing. The findings indicated that trench‐type drainage was more effective in reducing soil moisture due to its higher drainage water volume compared to other systems. The time required for the topsoil to reach its lower plastic limit in the subsurface, trench‐type and shallow surface drainage systems was 14, 11 and 15 h after the depletion of excess water over the soil surface, respectively. Although shallow surface drainage represented faster depletion of excess water, trench‐type drainage eventually proved to be the most effective alternative for providing appropriate qualifications for secondary cultivation. Crack areas on the soil surface were twice as extensive in trench‐type and subsurface drainage systems as in shallow surface drainage systems, indicating their superior performance.

Transcription factor CmHSFA4-CmMYBS3 complex enhances salt tolerance in chrysanthemum by repressing CmMYB121 expression.

Excessive soil salinity not only hampers plant growth and development but can also lead to plant death. Previously, we found that heat-shock factor A4 (CmHSFA4) enhances the tolerance of chrysanthemum (Chrysanthemum morifolium) to salt. However, the underlying molecular mechanism remains unclear. In this study, we identified a candidate MYB transcription factor, CmMYB121, which responded to salt stress. We observed that the CmMYB121 transcription is suppressed by CmHSFA4. Moreover, overexpression of CmMYB121 exacerbated chrysanthemum sensitivity to salt stress. CmHSFA4 directly bound to the promoter of CmMYB121 at the heat-shock element. Protein-protein interaction assays identified an interaction between CmHSFA4 and CmMYBS3, a transcriptional repressor, and recruited the corepressor TOPLESS (CmTPL) to inhibit CmMYB121 transcription by impairing the H3 and H4 histone acetylation levels of CmMYB121. Our study demonstrated that a CmHSFA4-CmMYBS3-CmTPL complex modulates CmMYB121 expression, consequently regulating the tolerance of chrysanthemum to salt. The findings shed light on the responses of plants to salt stress.

Effect of pericarp removal on buckwheat seed respiration and germination under submerged conditions

ABSTRACT Excessive soil moisture (ESM) is a critical factor limiting the productivity of buckwheat (Fagopyrum esculentum Moench) because ESM inhibits its germination. This study investigated the effects of pericarp and seed respiration on reduced buckwheat germination caused by submergence. Seeds were submerged prior to germination testing under aerobic conditions. The effects of days of submergence treatment and pericarp removal on germination percentage were significant. The germination percentage in the 3-day submergence treatment was low in seeds with pericarp (P+) but was significantly improved in seeds without pericarp (P−). Pericarp removal also improved the germination percentage of non-submerged seeds. Pericarp thickness and pericarp/seed dry weight ratio (PWR) were not significantly correlated with the germination percentage in 3-day submerged P+. However, both were significantly negatively correlated with the germination percentage in 1-day submerged P+. Seed respiration rate in water, measured on seeds not submerged before measurement, was significantly higher in P− than in P+; that of P− increased with time, while that of P+ showed a decreasing trend with time beyond the first 24 h. Seed respiration rate during the first 24 h was significantly negatively correlated with germination percentage in 3-day submerged P+. A negative influence of pericarp thickness and PWR on the seed respiration rate of P+ during the first 24 h was suggested. Suppressed seed respiration by the pericarp is probably a cause of inhibited buckwheat germination under ESM conditions.