In the rural scenario, cow-excreta-based bio-fertilizer development necessitates the utilization of cheaper protein and sugar sources such as jaggery and gram flour respectively. This article addresses the screening and identification of critical precursors associated with the development of low-cost and macronutrient-rich cow dung-based Jeevamrutha bio-fertilizer solid formulations. Plackett-Burman Design (PBD) statistical tool was deployed to screen and identify critical precursors among jaggery (A), gram flour (B), moisture content (C), soil (D) and pH (E). Two diverse parametric ranges (SH and SL) were considered to evaluate the chosen parametric range on the screening outcomes using Minitab 16.0 (USA). Ammonium nitrogen (AN) and phosphate (P) were considered as response variables in the conducted investigations. Thereby, twenty-four duplicate sets of experiments were performed from May to July. For a variation in A-C, D and E from 0.005 to 0.04 kg/L, 0.05–0.4 kg and 3–8, the AN and P varied from 49.8 ± 0.18 to 388.6 ± 0.73 and 606.5 ± 3.08 to 1248.1 ± 1.89 mg/L respectively. Pareto charts associated along with analysis of variance (ANOVA) characteristics affirmed high confidence level (95%), lower p-value (<0.05), higher F-value for screened parameters, etc. Best screened parameters were identified by PBD for their compositional fitness towards the improvement of nutrient content in the Jeevamrutha formulation. The major findings of this work shall contribute towards enhanced sustainability of the rural economy and minimized utility of hazardous chemical fertilizers for the sustained restoration of soil health and quality.
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