Soil microbial properties are known to exhibit high spatial and temporal variability, which can hinder our understanding of the effects of agricultural management on soil microbial activities, populations and communities. However, if this variability is explicitly considered in soil sampling schemes, experimental results can help us better understand soil microbial properties. In this initial assessment of soil resources and microbial properties in a 16-ha landscape planted to corn, we hypothesized that soil microbial properties will vary by soil type. We defined soil type based on drainage class (4 levels), series (7 levels), map unit (11 levels), and texture of the Ap horizon (3 levels). We took soil samples from 16 map units in April, June, August, and October and measured 12 physical and chemical properties, and 27 microbial properties on at least one of these dates. Soil physical and chemical properties generally varied with soil type and canonical discriminant analyses showed that soil drainage classes, series, and map units were delineated by a similar set of soil properties (total C, total N, moisture, pH, equivalent CEC (CEC e), and available P). Ap texture classes were delineated by a different set of soil properties (available K, Mg, and Ca and CEC e). A number of soil microbial properties varied by soil type, but, in general, a smaller proportion of measured soil microbial properties showed soil type effects compared to the proportion of soil physical and chemical properties that showed soil type effects. Method of soil classification strongly influenced which soil microbial properties varied by soil type. However, soil microbial activities, population sizes, and community structures were generally greater or most unique in mid-range soils regardless of how soils were classified, possibly because soil moisture was optimal for microbiological activity and growth in these soils. Except at the level of drainage class, there was little consistency between patterns of variation in soil physical and chemical properties and patterns of variation in soil microbial properties among soil types. Thus, different soil sampling strategies may be required when accounting for variation in soil physical and chemical properties than when accounting for soil microbial properties at the landscape level. While sampling soils according to soil survey-defined map units indicated that some soil microbial properties vary by soil type, a number of limitations to this approach are discussed. To improve upon results presented here, future efforts to quantify landscape level variation of soil microbial properties should probably be sampled at a finer scale that explicitly accounts for soil properties that vary at the sub-map unit level, including terrain attributes, microtopography, soil texture, soil bulk density, and other factors that impact soil temperature and moisture dynamics.
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