Sodium Pertechnetate Research Articles

Assessing functional suitability of a lyophilized formulation containing designed ankyrin repeat proteins for radionuclide imaging of HER2/neu overexpression in malignant tumors

Aim. To study in vitro and in vivo the functional suitability of 99mTc-labeled lyophilized formulation containing designed ankyrin repeat protein (DARPin) G3-(GGGS)3Cys for radionuclide imaging of HER2/neu overexpression in malignant tumors.Materials and methods. To create a targeted protein, a modified genetic construct with the sequence encoding DARPin G3-(GGGS)3Cys was used. To generate the experimental probe, we used a lyophilized formulation containing DARPin G3-(GGGS)3Cys with auxiliary substances and 99mTc sodium pertechnetate (500 MBq) incubated at 60 °C for 30 min. Radiochemical purity of 99mTc-G3-(GGGS)3Cys was analyzed by thin-layer radiochromatography. SKOV-3, BT-474, and DU-145 cell lines were used to test binding specificity in vitro. The dissociation constant was determined via a saturation binding assay on SKOV-3 cells with a range of protein concentrations from 0.2 to 40 nM. Nu/j mice bearing HER2-positive SKOV-3 xenografts and HER2-negative Ramos xenografts were used to evaluate the targeting properties and biodistribution.Results. A radiocomplex based on 99mTc and a lyophilized formulation with DARPin G3-(GGGS)3Cys was obtained with the radiochemical purity of more than 96%. Binding of 99mTc-G3-(GGGS)3Cys to the cells was specific (KD 3.9 ± 0.5 nM) and proportional to the level of HER2/neu expression in the cells. The uptake of 99mTc-G3-(GGGS)3Cys in SKOV-3 xenografts was significantly higher than in Ramos xenografts. 99mTc-G3-(GGGS)3Cys demonstrated rapid blood and renal clearance and had low activity in the salivary glands and stomach. Liver uptake was about 5–7%ID/g. In addition, 99mTc-G3-(GGGS)3Cys exhibited very low uptakes in the lungs, muscles, small intestine, and bones.Conclusion. The 99mTc-labeled lyophilized formulation with DARPin G3-(GGGS)3Cys is functionally suitable for imaging HER2/neu overexpression in tumors, as it binds specifically to the receptor, is stable in vivo, and has favorable biodistribution in organs and tissues. The radiocomplex based on 99mTc-G3-(GGGS)3Cys was obtained by a simple method with high radiochemical purity.

Preparation and Study of Stability Pharmaceutical Composition PSMA 11 and Radiopharmaceutical 99mtc PSMA 11 Labeled With 99mtc Radionuclide

This work is devoted to the study of the synthesis of a pharmaceutical composition (PhC) [PSMA-11] in the form of a lyophilisate with optimization of the amount of ingredients and sodium pertechnetate (Na99mTcO4) from the 99mTc Generator to obtain the radiopharmaceutical [ 99mTc]-PSMA-11 with the highest possible radiochemical yield (RCY) and radiochemical purity (RCP). The stability of radiopharmaceutical [99mТс]- PSMA-11 in 0.9% NaCl solution and in human serum was determined. The preliminary shelf life of PhC [PSMA-11] was determined and set to 6 months.

Pharmacoeconomic study of fluorescent lymphography and radionuclide diagnostics methods for sentinel lymph node detection in breast cancer

Background. Sentinel lymph node (SLN) biopsy is a reliable diagnostic method used to assess the spread of the malignant process in regional lymph nodes. Currently, this method is of great importance in treatment planning for early breast cancer and allows to avoid immediate and long-term complications of lymph node dissection. SLN detection can be performed using the radionuclide method, the disadvantages of which are associated with the peculiarities of working with radiopharmaceuticals and additional costs when using them. An alternative method is fluorescence lymphography (FL) with indocyanine green.Objective: performing a pharmacoeconomical study (cost-effectiveness analysis) comparing the cost-effectiveness of methods for detecting SLN in breast cancer: radionuclide diagnosis (RD) using a radiopharmaceutical based on sodium pertechnetate [99mТс] and FL using indocyanine green (Life Sciences OCPK LLC, Russia) and "IC GOR System" device (Central Design Bureau of Information and Control Systems JSC, Russia).Material and methods. The values of the cost-effectiveness ratio (CER) were calculated and compared for the two indicated methods of detecting SLN in breast cancer. Information on the RD method clinical efficacy was taken from the published data. SLN detection by the FL method with the use of indocyanine green and domestic apparatus was performed on the basis of the Regional Clinical Oncology Dispensary (Ryazan) in 53 patients with breast cancer. To assess the reliability of the obtained results a deterministic multivariate sensitivity analysis was performed.Results. The obtained CERFL values were significantly lower than the median CERRD values by average of 43.29% (p<0.001): from 40.36% (p<0.001) to 44.96% (p<0.001). The geometric mean CERFL was lower than CERRD by 39,40% (from 37.99% to 42.80%), and their 95% confidence intervals (CI) did not intersect. Deterministic multivariate sensitivity analysis demonstrated robustness of the obtained data to the transformation of RD cost parameters. Nevertheless the geometric mean and its 95% CI of CERRD and CERFL did not intersect under any circumstances.Conclusion. According to the results of a pharmacoeconomic study, it is more economically profitable to detect SLN in breast cancer using indocyanine green and a domestic device for FL, as its costs per unit of efficiency were 38–45% lower than for RD using a radiopharmaceutical based on sodium pertechnetate [99mТс].

SELECTION OF A LEACHING SYSTEM FOR THE EXTRACTION OF MOLYBDENUM-98 FROM USED <sup>99</sup>Mo/<sup>99m</sup>Tс GEL GENERATORS

The production of the radiopharmaceutical “Sodium pertechnetate 99mTc, solution for injection” from the transported 99mTc generator is a complex multi-stage process that includes the collection and disposal of radioactive waste generated at various technological stages of production, as well as after the return of the generator used in clinics. Currently, natural molybdenum oxide is used for the development of the parent isotope 99Mo at the WWR-K reactor, but as the need for 99mTc generators in Kazakhstan increases, the question arises about the use of expensive molybdenum oxide enriched with the isotope 98Mo, therefore, the task of regenerating irradiated molybdenum arises. The paper presents experimental data on the choice of an optimal leaching system for the isolation of molybdenum-98 from spent 99Mo/99mTc generators.

Cell Lysis, labeling efficacy and biodistribution of intravenously administered Technetium-99 m labeled multifunctional zirconia nanoparticles in animal model

Nanoparticles, to be used in biomedical applications, have been the subject of extensive investigation in recent years. Recently, zirconia and iron have gained significant relevance to the field due to their superior mechanical and structural qualities as well as their numerous biological applications. In this research, Fe3O4-stabilized zirconia nanopowders are fabricated using a low-cost sol–gel approach. Iron oxide sol is added in 1–10 wt% increments to a 0.1 M zirconia sol. The obtained crystallite size values match well with previously reported values for the tetragonally stable (t-zirconia) phase. Hardness values of approximately 1278 HV, along with fracture toughness values of ∼ 24.48 MPa.m-1/2 are observed for stable tetragonal zirconia. Results from cell lysis experiments indicate that the synthesized nanoparticles have the potential to combat cancerous cells. An encapsulation efficacy of up to ∼ 73% is observed after a 120-minute time period. Radio labeling techniques are employed to label the as-synthesized zirconia nanoparticles using Sodium Pertechnetate (Na99mTcO4) labeled radiopharmaceuticals, allowing for biodistribution assessment. Multiple CT scans of rabbits are performed to obtain accurate results and evaluate their survival. Studies show that radiolabeled nanoparticles are highly absorbed in the animals' bladders, making them a promising choice for tumor therapy. Various physical properties of the animals are periodically examined after administration of the nanoparticle-based injection for several months, and no abnormalities are found. The results obtained through these characterizations indicate that these nanoparticles can be used as future therapeutic agents as well as drug carriers.

Effectiveness of polymeric gloves in radioprotection against contamination in nuclear medicine

When handling unsealed radioactive sources, radiological protection attention must be taken to avoid unnecessary exposure and radioactive contaminations, and an important and necessary practice to prevent such contaminations is the use of gloves when handling these sources. The present work aimed to determine the effectiveness of contamination protection provided by different types of disposable polymeric gloves used in Nuclear Medicine Service in Clinic Hospital of Porto Alegre, testing the main radiopharmaceuticals used at this site: [99mTc]sodium pertechnetate, [18F]FDG and [131I]sodium iodide. The analysis was performed using the wipe test inside gloves intentionally contaminated on the outside with these radiopharmaceuticals. The radiation detector used to measure the contamination was a NaI(Tl) scintillator well-type counter. The results indicate that three types of gloves analyzed protect the user from [99mTc]sodium pertechnetate and [18F]FDG contamination, for permanence times with the glove after contamination for up to 15 min (interval tested). For [131I]sodium iodide, gloves are completely effective in protection as long as they are used for a time interval after contamination of the external surface of up to: Latex – 5 min; Vinyl – 5 min; Nitrile – 10 min. Among them, the nitrile glove are the most effective, since contamination was not observed on the inner face for times equal to or less than 10 min; and, for an interval of 15 min, the percentage of permeation obtained was lower than the other two types: 3.3 times lower than vinyl glove permeation and 1.3 times lower of the latex glove permeation. It was also possible to estimate the skin dose rate due to contamination caused by iodine permeation for each glove case and time tested.

Pharmacokinetics and biodistribution studies of [99mTc]-Labeled ZIF-8 nanoparticles to pave the way for image-guided drug delivery and theranostics

The last few years have witnessed an increasing interest in using metal-organic frameworks (MOFs), a newly emerging class of highly porous nanomaterials. Countless studies have been performed on the synthesis, characterization, and applications of these nanomaterials. However, tracking these nanoparticles' in vivo fate is of great importance for practical applications. Herein, we conducted a profound investigation of the pharmacokinetics and biodistribution of MOF nanoparticles. To this end, Zn-based MOF (i.e., ZIF-8 nanoparticles) were synthesized and radiolabeled with technetium-99 m [99mTc] radioisotope with high labeling efficiency. The prepared nanoparticles exhibited high in-vitro stability. The biodistribution and pharmacokinetic studies were performed in animals, and the data were compared with free [99mTc]. The biodistribution was investigated using the single-photon emission computed tomography (SPECT) imaging technique. The highest ZIF-8 NPs accumulation was observed in the lung, while free [99mTc] was mainly accumulated in the animal's stomach. The results revealed that the half-life of [99mTc]-ZIF-8 nanoparticles in the blood was almost twice that of sodium pertechnetate. Accordingly, due to their high pulmonary biodistribution, ZIF-8 NPs can considerably improve the pharmacokinetics of encapsulated agents and be used for lung targeting. This study paves the way for developing favorable nanocarriers for diagnostic, therapeutic, and theranostic applications.

Microleakage Study of a Bulk Fill over an Uncured Adhesive System

This study aimed to evaluate and compare the microleakage of composite resin restorations under a total-etch adhesive system applied with two different techniques, with and without cure. Cavities were made on the buccal surface of twenty-six intact teeth and subsequently restored with bulk-fill flow composite resin (SDR®® flow+) and conventional resin (Spectra™ ST HV). Two experimental groups were created, one where the total-etch adhesive (Prime & Bond Active®) was cured and another where the adhesive was not cured before placing the flowable resin. Two control groups were also created, negative and positive. After the restorations were finished the, teeth were submerged in a solution of sodium pertechnetate (99mTcNaO4) for 3 h to evaluate the microleakage. The results showed more infiltration of radioisotopes characterized by the highest total count values of microleakage in group 1 (cured adhesive) and in the positive control. Group 2 (noncured adhesive) and the negative control showed lower values. We therefore considered that these groups were similar since their p-value was less than 0.05, with no statistically significant difference. Group 4 showed a statistically significant increase in relation to group 3 (p = 0.027). We concluded that the method of bulk-fill composite resins with noncured adhesive agents can have a positive effect on the longevity of bond strengths, with reduced microleakage.

Development and technical validation of an ultrasound nebulizer to deliver intraperitoneal pressurized aerosols in a rat colon cancer peritoneal metastases model

Background/aimTo develop and validate a nebulizer device for anti-cancer research on pressurized intraperitoneal aerosol supply in a preclinical peritoneal metastases (PM) rat model.Material and methodsFor aerosol generation, an ultrasonic nebulizer (USN) was modified. Aerosol analyses were performed ex-vivo by laser diffraction spectrometry (LDS). Intraperitoneal (IP) 99mtechnetium sodium pertechnetate (99mTc) aerosol distribution and deposition were quantified by in-vivo single photon emission computed tomography (SPECT/CT) and compared to liquid IP instillation of equivalent volume/doses of 99mTc with and without capnoperitoneum. PM was induced by IP injection of HCT116-Luc2 human colon cancer cells in immunosuppressed RNU rats. Tumor growth was monitored by bioluminescence imaging (BLI), 18F-FDG positron emission tomography (PET) and tissues examination at necropsy.ResultsThe USN was able to establish a stable and reproducible capnoperitoneum at a pressure of 8 to 10 mmHg. LDS showed that the USN provides a polydisperse and monomodal aerosol with a volume-weighted diameter of 2.6 μm. At a CO2 flow rate of 2 L/min with an IP residence time of 3.9 s, the highest drug deposition efficiency was found to be 15 wt.-%. In comparison to liquid instillation, nebulization showed the most homogeneous IP spatial drug deposition. Compared to BLI, 18F-FDG-PET was more sensitive to detect smaller PM nodules measuring only 1–2 mm in diameter. BLI, 18F-FDG PET and necropsy analyses showed relevant PM in all animals.ConclusionsThe USN together with the PM rat model are suitable for robust and species-specific preclinical pharmacological studies regarding intraperitoneal delivery of pressurized aerosolized drugs and cancer research.

A preliminary biodistribution study of [99mTc]sodium pertechnetate prepared from an electron linear accelerator and activated carbon-based 99mTc generator

IntroductionProduction of 99Mo/99mTc using an electron linear accelerator (linac) and activated carbon (AC)-based 99mTc generator (linac-AC) is an alternative approach to the conventional fission production of 99Mo/99mTc. As a preliminary investigation of the clinical applicability of a linac-AC-derived 99mTc radiopharmaceutical, the biodistribution of linac-AC-derived [99mTc]sodium pertechnetate ([99mTc]NaTcO4) was measured and compared against fission-derived [99mTc]NaTcO4 at one time point. Methods99Mo was produced by irradiating nonenriched MoO3 targets with bremsstrahlung photons generated from 55.5-MeV linac electron beams. 99mTc was then separated and purified from the 99Mo using an AC-based 99mTc generator. Subsequently, biodistribution of the linac-AC-derived [99mTc]NaTcO4 in healthy female Slc:ICR mice (n = 6) was measured by dissection and compared with that of fission-derived [99mTc]NaTcO4 (n = 4) at 30 min after injection. ResultsThe two types of [99mTc]NaTcO4 exhibited similar biodistribution in all the organs and tissues examined: the uptakes of [99mTc]NaTcO4 prepared from the linac-AC method and those prepared from the fission method were 138.9 ± 69.9%ID/g and 160.6 ± 49.2%ID/g in the thyroids, respectively, 33.4 ± 5.5%ID/g and 29.4 ± 9.1%ID/g in the salivary glands, respectively, and less than 10%ID/g in blood and all the other organs. No adverse effects were observed in the mice administered with either [99mTc]NaTcO4. ConclusionThe clinical applicability of linac-AC-derived [99mTc]NaTcO4 was suggested by its similar biodistribution with fission-derived [99mTc]NaTcO4 at one time point. Further biodistribution studies at multiple time points are encouraged to demonstrate the bioequivalence between linac-AC- and fission-derived [99mTc]NaTcO4.

Comparative Study of Two Identical Industrial Digesters Using Radiotracer‐Based Residence Time Distribution Measurement

AbstractRadiotracer‐based residence time distribution (RTD) measurements were performed for two identical wheat straw pulping digesters to evaluate and compare their performances. The radiotracer technetium (99mTc) as sodium pertechnetate was used to follow the liquid phase inside the digester. The product quality of both digesters was not identical for similar sets of operating conditions. Temperature, pressure, and mass flow rates were the influencing parameters, and the experimental and theoretical mean residence time (MRT) were compared. A significant amount of flow abnormalities was observed in the second digester as compared to the first one, including channeling, dead volume, scaling, and bypassing. The axial dispersion model and tank in series with the back‐mixing model were suitable for modeling the obtained RTD data.

Effect of Residual Volume in a Three-way Stopper and a Syringe on Actual Dose of Radiopharmaceuticals: Effect of Different Three-way Stoppers and Washing

The purpose of this study were to evaluate the residual volume of radiopharmaceuticals in the three-way stopper and syringe used during radiopharmaceutical administration and the effect of washing. The three-way stoppers were a top injector tube, a top three-way stopper, and a Nipro three-way stopper with a needle. Sodium pertechnetate [99mTc] injection (99mTcO4-) was used to compare the residual volume of radiopharmaceuticals in the three-way stopper and syringe without and with washing. Clinically, 137 patients who underwent cerebral blood flow scintigraphy, dopamine transporter scintigraphy, and bone scintigraphy were included. N-isopropyl-p-[123I]iodoamphetamine (123I-IMP), 123I-N-ω-fluoropropyl-2β-carboxymethoxy-3β-(4-iodophenyl)nortropane (123I-FP-CIT), and 99mTc-methylene diphosphonate (99mTc-MDP) were used to compare the residual volume of radiopharmaceuticals in the three-way stopper and syringe without and with washing. The residual volume depended on the type of three-way stopper and radiopharmaceutical used. The residual volume could be reduced by washing, but the effect depended on the type of three-way stopper and radiopharmaceutical used. The residual volume of radiopharmaceuticals in three-way stoppers and syringes can be determined and subtracted to achieve more accurate dose control.

Apical Sealing Ability of Two Calcium Silicate-Based Sealers Using a Radioactive Isotope Method: An In Vitro Apexification Model.

The aim of this study was to evaluate and compare the sealing ability of two calcium silicate-based sealers (TotalFill BC RRM Fast Set Putty and White ProRoot MTA) when used as apical plugs in immature teeth through nuclear medicine. Single-rooted extracted teeth (n = 34) had their crowns and root tip sectioned to obtain 14 mm long root segments to simulate an in vitro apexification model. Were created two experimental groups, namely MTA (n = 12) and BC (n = 12), and two control groups, PG (positive group, n = 5) and NG (negative group, n = 5). On the 4th day after placing the respective apical plug, the apical portions of the teeth were submerged in a solution of sodium pertechnetate (99mTcNaO4) for 3 h. Statistical analysis showed a significant difference between the MTA group and the controls (p < 0.05). The BC group had a significant difference regarding the negative control (p < 0.001) but showed no statistical significance regarding the positive control (p = 0.168). There was a statistically significant difference (p = 0.009) between the BC group (7335.8 ± 2755.5) and the MTA group (4059.1 ± 1231.1), where the last showed less infiltration. Within the limitations of this study, White ProRoot MTA had a significantly better sealing ability than TotalFill BC RRM Fast Set Putty.

Investigation of transport of radionuclide in a thermal stratification test facility using radiotracer technique

A radiotracer investigation was carried out in a Thermal Stratification Test Facility (TSTF) with objectives of investigating the dispersion and diffusion of radionuclide and effectiveness of the thermocline to minimize the radionuclide content in the hot water layer. Technetium-99m (99mTc) as sodium pertechnetate was used as a radiotracer in the investigation. Qualitative analysis showed that a thermocline is formed within the TSTF and is effective in preventing the transport of radionuclide from bottom section to the top section of the facility. It was found that the radiotracer injected at the bottom of the pool took about 17.4 h to disperse from bottom to the top of the facility. The results of the investigation helped in understanding the effectiveness of hot water layer and thus to minimize the pool top radiation levels.

Transferability of a two-strip method for the quality control of technetium-99m mercaptoacetyltriglycine ([99mTc]Tc-MAG3)

ObjectivesTechnetium-99m mercapto-acetyl-triglycine ([99mTc]Tc-MAG3) is a radiopharmaceutical diagnostic agent used in nuclear medicine intended for the exploration of nephrological and urological disorders. Patient safety and reliability of this imaging procedure especially...

Residence time distribution study in a pilot-scale liquid phase catalytic exchange (LPCE) column packed with a mixture of hydrophobic and hydrophilic catalysts

Residence time distribution (RTD) measurements were carried out in a packed bed column designed for exchange of hydrogen isotopes. The main objective of the study was to characterize the liquid phase mixing under various processes and operating conditions. The packed bed was composed of a mixture of two different types of catalytic packing materials, i.e., a hydrophobic material and a hydrophilic material. Technitium-99m (99mTc) as sodium pertechnetate was used as a radiotracer for RTD measurements. From the measured RTD curves, mean residence times (MRTs), liquid holdup and degree of mixing of liquid phase were evaluated. An axial dispersion model exchange with stagnant zones was used to simulate the measured RTD curves. The results of model simulation showed that volume fraction of hydrophobic to hydrophilic packing and gas/liquid superficial velocities affect the liquid holdup, bed pressure drop and liquid phase dispersion/mixing characteristics. The results of the present study will help to screen packing, optimize the volume of the packing fractions, design and construct the catalyst and optimize the operating conditions for scale up of the isotope exchange process.

5-Fluorouracil Loaded Orally Administered WGA-decorated Poly(lacticco- glycolic Acid) Nanoparticles for Treatment of Colorectal Cancer: In Vivo Evaluation

Background:: Several studies have suggested the potential aptitude of polylactic-co-glycolic acid (PLGA)-derived nanoparticles (NPs) to improve the antitumor efficacy of anticancer drugs against colon cancer. Further, conjugation of lectins over the surface of the NPs may ameliorate interaction and thus enhance the attachment of NPs with receptors. Objective:: The main goal of the study was to prepare and evaluate the targeting potential (in vivo) of the optimized NPs against colorectal cancer. Methods:: The 5-fluorouracil (5-FU) loaded and wheat germ agglutinin (WGA)-conjugated PLGA- NPs (WFUNPs) were prepared and then they were evaluated in vivo for targeting aptitude of formulation using gamma scintigraphy after oral delivery. The WGA-conjugated and non-conjugated optimized NPs were compared for any significant results. Further, optimized formulations were also assessed for different parameters such as radiolabeling efficiency, sodium pertechnetate uptake, stability of NPs, and organ distribution study. Results:: Findings suggested prolonged retention of 99mTc-tagged WFUNPs in the colonic region after 24 h study. Eventually, the outcome from conjugated formulation revealed enhanced bioavailability of the drug in blood plasma for up to 24 h. Conclusion:: In conclusion, WGA-conjugation to NPs could improve the performance of the PLGA- NPs in the treatment of colorectal cancer.

Radiolabeled Tedizolid Phosphate Liposomes for Topical Application: Design, Characterization, and Evaluation of Cellular Binding Capacity.

Nowadays, the incidence of acute bacterial skin and skin structure infection (ABSSSI) is increasing. The increased bioavailability and reduced drug resistance of antibiotics are crucial to obtain a more effective treatment response in these infections. These favorable properties could be achieved by different drug delivery systems such as liposomes. In this study, nanosized, radiolabeled tedizolid phosphate liposomal formulations were prepared and evaluated with their in vitro cellular binding capacity and biocompatible profile for topical treatment of ABSSSI. Liposomes were characterized by evaluation of their visual inspection, particle size (about 190-270nm), zeta potential value (around 0), and encapsulation efficiency (nearly 10%). The release rate of tedizolid phosphate from liposomes was also studied using dialysis membranes and evaluated kinetically. The stability of formulations was observed at three different temperatures and humidity conditions for 28days. Afterward, liposomes were labeled with 99mTc, and the optimal amount of reducing agent (stannous chloride) was determined as 500μg in this direct labeling procedure. All liposome formulations were successfully radiolabeled with high efficiency and exhibited high radiochemical purity (> 80%) during 6h in different media. Furthermore, the cellular bindings of liposomal formulations were evaluated in human skin fibroblast cells by measuring the radioactivity. Higher radioactivity values were obtained in CCD-1070Sk cells incubated by liposome formulations compared to sodium pertechnetate. This finding suggested that liposomal formulation increased the cellular binding of radioactivity. By the result of our study, nanosized, tedizolid phosphate encapsulated liposome formulation was found to be a favorable carrier system in the treatment of ABSSSI.

Use of 99m Tc in The Field of Radiofarmation: A Review

A B S T R A C TTechnetium-99m ( 99m Tc) has been applied in nuclear medicine as a radiopharmacyfor both diagnosis and therapy. 99m Tc is obtained from a 99 Mo/ 99m Tc (half-life 66 h)generator in the form of sodium pertechnetate (Na[ 99m TcO 4 ]) by decaying to 99 Tc for 6hours and emitting gamma energy rays (Eɤ = 140 keV). This radionuclide has anelectron configuration of 4d 5 5s 2 , which will form complexes with different ligandsand have oxidation rates from +1 to +7. The coordinated complex of technetium-99mhas been utilized in nuclear medicine in tissues and organs (thyroid, red and whiteblood cells, kidneys, brain, myocardial, and bone). The resulting kit production musthave based on Good Manufacturing Practice, which consists of batch planning,washing, sterilization of glassware and stopper, starting material, preparation oflarge quantities of the solution, sterile filtration, dispensing, crimping, a summary ofprocess control, quarantine, packaging and leaving the production premises.

Importance of gamma radiation using sodium pertecnetate (TC 99m) and iodine 131 as a suggestion in the treatment of COVID-19

Objective: to point out the importance of nuclear medicine for the diagnosis and therapy of COVID-19, in addition to reporting the efficacy of treatment with radioisotopes (sodium pertechnetate and iodine 131) in individuals infected with SARS-CoV-2 causing viral sterility and preventing its mutagenesis and ensuring the absence of adverse reactions. Methodology: This is a review of the narrative-type literature, prepared between June and September 2020, using the following databases: PubMed, SciELO and Lilacs. Thus, there was the inclusion of articles published between 1971 and 2020. Results and Discussion: The use of gamma and neutron radiation for the purpose of eliminating viral pathogens has been extensively researched in American studies, initially focusing on H1N1. The substance of Sodium Pertecnate, suggested in this study, and iodo 131 (designed to act as prevention in the cervical region) have the potential for satisfactory responses in the therapeutic treatment of COVID-19 due to the innocuous, painless and free of any effect methodology therefore, offering greater security to the treatment. Inferring in the replication of the coronavirus through the electrolysis of its fatty structure, since with the sterilization of its RNA molecule there will be no replication. Final Consideration: Based on the effectiveness of this method in sterilization protocols and action on various types of microorganisms, especially other viruses that affect the respiratory system, it is suggested as a safe, low-cost and low-dose radioactivity method for the patient, when used doses similar to those of the diagnostic and therapeutic practice in Nuclear Medicine. It should be noted that the administration protocol must be in accordance with the standard already recommended clinically, with full guarantee of the absence of associated effects. For better clarification, there is a need for more specific research within the scope of SARS-CoV-2.