SOD Group Research Articles

Transcriptome analysis suggests broad jejunal alterations in Linghu’s obesity-diarrhea syndrome: A pilot study

BACKGROUND Obesity is associated with a significantly increased risk for chronic diarrhea, which has been proposed as Linghu’s obesity-diarrhea syndrome (ODS); however, its molecular mechanisms are largely unknown. AIM To reveal the transcriptomic changes in the jejunum involved in ODS. METHODS In a cohort of 6 ODS patients (JOD group), 6 obese people without diarrhea (JO group), and 6 healthy controls (JC group), high-throughput sequencing and bioinformatics analyses were performed to identify jejunal mucosal mRNA expression alterations and dysfunctional biological processes. In another cohort of 16 ODS patients (SOD group), 16 obese people without diarrhea (SO group), and 16 healthy controls (SC group), serum diamine oxidase (DAO) and D-lactate (D-LA) concentrations were detected to assess changes in intestinal barrier function. RESULTS The gene expression profiles of jejunal mucosa in the JO and JC groups were similar, with only 1 differentially expressed gene (DEG). The gene expression profile of the JOD group was significantly changed, with 411 DEGs compared with the JO group and 211 DEGs compared with the JC group, 129 of which overlapped. The enrichment analysis of these DEGs showed that the biological processes such as digestion, absorption, and transport of nutrients (especially lipids) tended to be up-regulated in the JOD group, while the biological processes such as rRNA processing, mitochondrial translation, antimicrobial humoral response, DNA replication, and DNA repair tended to be down-regulated in the JOD group. Eight DEGs (CDT1 , NHP2 , EXOSC5 , EPN3 , NME1 , REG3A , PLA2G2A , and PRSS2 ) may play a key regulatory role in the pathological process of ODS, and their expression levels were significantly decreased in ODS patients (P < 0.001). In the second cohort, compared with healthy controls, the levels of serum intestinal barrier function markers (DAO and D-LA) were significantly increased in all obese individuals (P < 0.01), but were higher in the SOD group than in the SO group (P < 0.001). CONCLUSION Compared with healthy controls and obese individuals without diarrhea, patients with Linghu’s ODS had extensive transcriptomic changes in the jejunal mucosa, likely affecting intestinal barrier function and thus contributing to the obesity and chronic diarrhea phenotypes.

Destandau Tekni̇ğiyle Lomber Diskektomi̇ Kısa Vadede Daha İyi Sonuçlar Veri̇yor

Background: The long-term results of patients who underwent lumbar discectomy using the Desdandau technique (EDDT) and standard open technique (SOD) were compared. Materials and Methods: A retrospective screening was made of patients, aged 18-70 years, who underwent single-level lumbar discectomy between 2007 and 2011. A total of 52 patients met the study criteria and were separated into 2 groups according to the surgical technique used, the EDDT group (n: 27) and the SOD group (n:25). All patients were compared using Visual Analogue Scale (VAS), Oswestry Disability Index (ODI) and modified MacNab criteria. Results. The mean follow-up period was calculated as 158.2±9.2 months in the EDDT group and 161.2±11.1 months in the SOD group. The preoperative VAS and ODI values were observed to be similar in both groups (p=0.829); p=0.120 respectively). At the early postoperative visit, VAS and ODI values were lower in the EDDT group (VAS: EDDT: 2.2±0.6; SOD: 4.1±0.8, p<0.001; ODI: EDDT: 15.4±1.6, SOD: 29.1±1.9, p<0.001, respectively), however, there was no significant difference in VAS and ODI scores between the two groups at the last control (VAS: EDDT: 2.1±0.4, SOD: 2.4±0.5, p=0.078; ODI: EDDT: 14.6±2.1, SOD: 15.1±1.2, p=0.033, respectively). According to the modified MacNab criteria, good and excellent results were obtained in 88% of the SOD group and 92% of the EDDT group. Conclusion: The study found that the long-term results of both techniques were similar. However, the Destandau technique had better early results. The endoscopic method seems to be better in terms of early return to daily activities.

Impact of operation duration on short-term and long-term prognosis in patients undergoing radical colorectal surgery.

Purpose: The objective of this study was to evaluate the impact of operation duration on short- and long-term outcomes of colorectal cancer patients following surgical resection.Methods: 6224 consecutive patients who underwent radical colorectal surgery were retrospectively assessed and were divided into short operation duration group (SOD) and long operation duration group (LOD) according to the operation duration cutoff value of 110 minutes.Results: Compared with patients in LOD group, patients in SOD group had significantly lower total costs in hospital, reduced expenses for drugs and antibiotics, shorter length of stay (LOS) in hospital and in the ICU. Moreover, 5-year overall survival (OS) and disease-free survival (DFS) for patients in the SOD group were markedly higher than for patients in the LOD group. Mutivariate regression analysis indicated that longer operation duration was associated with poor prognosis, with a hazard ratio of 1.004 (1.003, 1.005) for OS and 1.005 (1.003, 1.006) for DFS. Finally, surgeons' qualifications had meaningful correlation with operation duration (r= 0.450).Conclusions: Operation duration is an independent risk factor for patients' short-term and long-term outcome after radical colorectal surgery. Improve the surgical skills of the surgeon may shorten the operation duration, and further improve the outcome for patients.

COMPARATIVE STUDY OF THE EFFECT OF SUPEROXIDE SOLUTION VERSUS CHLORHEXIDINE GLUCONATE SOLUTION IN SURGICAL WOUND DRESSING

Objective: The objective of the study is to compare the effect of superoxide solution (SOD) and chlorhexidine gluconate on wound healing. Methods: Hundred patients with different types of wounds were recruited in this study and randomized to two groups: Group A- SOD group and Group B- Chlorhexidine group. Observations were made regarding type of wounds, reduction of edema, culture of wounds, improvement, cure rates, and hospital stay. Results: Although there were no significant difference in the reduction of edema and swelling of wound in both groups, the cure rates were statistically better with SOD (p=0.001) and hospital stay was also less with the use of SOD (p=0.006). Conclusion: SOD treated case showed less morbidity, with more improvement, and more cure rate compared to chlorhexidine solution with statistically significant p-value. However, further research into the use of SOD and the cytotoxic nature of chlorhexidine in the treatment of various wounds is required.

Optical coherence tomography in the diagnosis of primary neurodegenerative diseases

AbstractBackgroundCurrently, the priority is the early diagnosis of dementia, which is due to an increase in the life expectancy of the population. In connection with the improvement of criteria of Alzheimer's disease on the basis of which a diagnosis is made only when severe cognitive deficit, prevented the diagnosis of documentah stages, although a neurodegenerative process is started before the onset of clinical symptoms, which should be treated with medications. Recently, the world community has been focused on identifying new predictors of cognitive deficits, as such, scientists ' hopes are focused on optical coherence tomography.Method90 patients were examined and divided into 3 groups: the first group included patients with Alzheimer's disease (NINCDS ADRDA), the second group included patients with vascular dementia(NINDS‐AIREN), and the third group ‐ patients with open‐angle glaucoma. The groups were comparable by gender, age, and baseline socioeconomic status. All patients underwent a comprehensive neurological and neuropsychological examination, as well as optical coherence tomography.ResultAccording to the results of OCT in the area of the optic disc, no statistically significant differences in the width of the neuroretinal margin were found in the groups. The results of OCT in the macular region revealed a decrease in the retinal thickness and inner plexiform layer in the upper and lower quadrants and bilateral region, as well as reducing the thickness of the nerve fibers of the ganglion layer in a lower quadrant and bilateral region in groups of AD and glaucoma, compared with SoD group (p <0.05). In the foveal region, there was a decrease in the thickness of the retina, the ganglion cell layer, and the inner plexiform layer of the retina in patients with SoD compared to patients with AD and glaucoma (p <0.05).Based on the data obtained, it can be concluded that in AD, degenerative retinal changes are observed in the perifoveal region, and in SoD, the process is more pronounced in the foveal (central) region of the retina.Conclusiona comprehensive ophthalmological examination can become one of the key and affordable methods of early diagnosis of AD.

Probiotic reduced the impact of phthalates and bisphenol A mixture on type 2 diabetes mellitus development: Merging bioinformatics with in vivo analysis

Linkage between bis(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), and bisphenol A (BPA) co-exposure and type 2 diabetes mellitus (T2DM), as well as ability of multi-strained probiotic to reduce DEHP, DBP and BPA mixture-induced oxidative damage in rat pancreas were investigated. The Comparative Toxicogenomics Database, Cytoscape software and ToppGene Suite were used for data-mining. Animals were sorted into seven groups (n = 6): (1) Control group: corn oil, (2) P: probiotic: Saccharomyces boulardii + Lactobacillus rhamnosus + Lactobacillus plantarum LP 6595 + Lactobacillus plantarum HEAL9; (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day, and (6) MIX: 50 mg/kg b.w./day DEHP + 50 mg/kg b.w/day DBP + 25 mg/kg b.w./day BPA; (7) MIX + P. Rats were sacrificed after 28 days of oral exposure. In silico investigation highlighted 44 DEHP, DBP and BPA mutual genes linked to the T2DM, while apoptosis and oxidative stress were highlighted as the main mechanisms of DEHP, DBP and BPA mixture-linked T2DM. In vivo experiment confirmed the presence of significant changes in redox status parameters (TOS, SOD and SH groups) only in the MIX group, indicating possible additive effects, while probiotic ameliorated mixture-induced redox status changes in rat pancreatic tissue.

Splanchnic occlusive disease predicts for spinal cord injury after open descending thoracic and thoracoabdominal aneurysm repair

ObjectiveIn the present study, we sought to discern the effects of splanchnic occlusive disease (SOD; renal, superior mesenteric, and/or celiac axis arteries) on spinal cord injury (SCI; paraparesis or paraplegia) and major adverse events (MAE) after descending thoracic aneurysm (DTA) and thoracoabdominal aortic aneurysm (TAAA) open repair. MethodsPatients who had undergone DTA/TAAA repair at our institution were dichotomized according to the presence of SOD, which was investigated as a predictive factor of our primary (SCI) and secondary (operative mortality, myocardial infarction, stroke, tracheostomy, de novo dialysis, MAE, survival) endpoints. Risk adjustment used both propensity score matching and multivariable logistic regression. ResultsFrom July 1997 to October 2019, 888 patients had undergone DTA/TAAA repair, of whom 19 were excluded from our analysis for missing data. SOD was absent in 712 patients and present in 157 patients. The patients with SOD had presented with a greater incidence of preoperative renal impairment (61 [38.9%] vs 175 [24.6%]; P < .01) and peripheral arterial disease (60 [38.2%] vs 162 [22.8%]; P < .01] and decreased left ventricular ejection fraction (45%; interquartile range, 10%; vs 50%; interquartile range, 4%; P < .01). The etiology of aortic disease was more frequently dissection in the SOD group (56.1% vs 43.7%) and more frequently nondissecting aneurysm in the non-SOD group (56.3% vs 43.9%; P < .01). Patients without SOD had presented with aneurysms more cranially located (DTA, 34.0% vs 7.6%; extent I TAAA, 44.0% vs 7.6%). In contrast, patients with SOD had presented with aneurysms more caudally located (extent II TAAA, 36.9% vs 8.6%; extent III TAAA, 30.6% vs 11.0%; extent IV TAAA, 17.2% vs 2.5%; P < .01). Propensity score matching led to 144 pairs, with SOD significantly associated with SCI (10 [6.9%] vs 2 [1.4%]; P = .03) and MAE (47 [32.6%] vs 26 [15%]; P < .01). Ten-year survival was reduced in those with SOD (31.5% vs 45.2%; P < .01). Conditional multivariable regression confirmed SOD to be a predictor of SCI in the matched sample (odds ratio, 6.60; P = .02). ConclusionsOur results have shown that SOD is a significant predictor of SCI in patients undergoing open DTA/TAAA repair. The investigation of measures to prolong neuronal ischemia tolerance (eg, hypothermia) is warranted for such patients.

Comparative assessment of the antioxidative defense system in subadult and adult anurans: A lesson from the Bufotes viridis toad

During their complex life cycle, anurans are faced with various physiological and biochemical demands that can result in increased free radical production. The antioxidative system (AOS), assumes a central role in protection from oxidative stress, and increased knowledge of its response would allow us to identify and quantify underlying costs of free radical production. In this study, we compared the patterns and levels of integration of the AOS during two life stages of Bufotes viridis toads from natural populations: young, fully developed, reproductively inactive subadults and reproductively active adults. All examined parameters (SOD, CAT, GSH-Px, GR, GST, GSH and SH groups) were measured in the liver, skin and muscle. The obtained results showed that: (1) subadult individuals possessed a more integrated AOS (a higher index of integration and number of significant correlations), based mostly on the coordinated action of SOD-CAT; (2) adult individuals used the GSH-Px/GSH system; (3) all examined tissues exhibited similar patterns of variation of AOS parameters; (4) significant correlations between the AOS and biometric parameters. Our results indicate that adult and subadult toads differed in the way they processed free radicals, implying that adults may be more susceptible to oxidative damage. The knowledge from this study will contribute to a better understanding of the AOS and how its activity is modulated in natural populations of anurans throughout their life.

Modified porcine surfactant enriched by recombinant human superoxide dismutase for experimental meconium aspiration syndrome

AimsCombination of exogenous surfactant with antioxidant enzyme recombinant human superoxide dismutase (rhSOD) was tested in the treatment of experimental meconium aspiration syndrome as oxidative processes play key role in its pathogenesis. Material and methodsYoung New Zealand rabbits were instilled by saline (Sal group) or by meconium suspension (Mec group). Some of meconium-instilled animals were treated by surfactant alone (Surf group) or surfactant in combination with rhSOD (Surf + SOD group) and oxygen-ventilated for 5 h. PaO2/FiO2, oxygenation index, oxygen saturation, PaCO2, ventilation efficiency index and alveolar-arterial gradient were evaluated every hour; post mortem, cells in bronchoalveolar lavage were counted, inflammatory and oxidative markers were assessed using ELISA in lung tissue homogenates. Key findingsExogenous surfactant combined with rhSOD improved oxygenation during the first hour after the treatment more than surfactant alone (p = 0.039 to 0.0001 vs. Mec and Surf group). Amelioration was also seen in CO2 elimination (p = 0.049 to 0.0096 vs. Mec group), alveolar-arterial gradient diminution (p = 0.024 to 0.0019 vs. Mec and Surf group), prevention of oxidative damage and cytokine production (p = 0.049 to 0.002 vs. Mec group). SignificanceIt seems that inhibition of oxidative signalization may be strong supporting factor in surfactant treatment of MAS.

The radiation protection role of heparin-SOD conjugate in irradiated mice

Heparin-SOD conjugate (Hep-SOD) was prepared by modifying Cu,Zn-SOD with heparin. An acute radiation-induced mouse injury model was constructed to study the radiation protection effects of Hep-SOD conjugate. Fifty-six mice were randomly divided into seven groups: (I) normal control group; (II) irradiated control group; (III) positive control group (amifostine group, 300 mg/kg); (IV) SOD group (35000 U/kg); (V) high dosage of Hep-SOD group (70000 U/kg); (VI) medium dosage of Hep-SOD group (35000 U/kg); (VII) low dosage of Hep-SOD group (17500 U/kg). Drugs were intraperitoneally injected into each mouse 1 h before radiation except for the normal control group. All the irradiated groups were irradiated with 6 Gy. Organ indices, haematopoietic function indices, peripheral blood cells, liver function test, oxidative stress state and pathological observation were detected to study the effects of Hep-SOD on irradiated mice. Results showed that bone marrow suppression of irradiated mice could be reduced when treated by Hep-SOD before radiation. Oxidative stress detection and pathological observation of the liver and intestine showed that the damage caused by radiation was relieved when mice were treated with Hep-SOD before radiation. This study shows a new direction to prevent organisms from the damage caused by radiation.

Maternal and postnatal high-fat diets with high ω6 : ω3 ratios affect the reproductive performance of male offspring in the mouse.

High-fat diets (HFDs) are an acknowledged risk factor for male subfertility, but the underlying mechanisms remain unclear. In the present study we compared the effects of two HFDs with different ω6:ω3 ratios, one enriched with soy oil (SOD; ω6:ω3=9.62) and another enriched with sunflower oil (SFOD; ω6:ω3=51.55), with those of a commercial diet (CD; ω6:ω3=19.87), supplied from pregnancy to adulthood, on morphometric parameters and reproductive performance in adult male mice (recommended ω6:ω3 for rodents=1-6). Bodyweight was significantly higher in the SFOD than CD group, and relative testicular weight was significantly lower in the SFOD than the other two groups. SFOD altered sperm performance: it reduced sperm viability (mean±s.e.m.; 76.00±1.35% vs 82.50±1.45% and 80.63±1.00% in the SFOD vs CD and SOD groups respectively; P<0.05) and increased the percentage of immature spermatozoa (71.88±7.17% vs 51.38±5.87% and 48.00±5.72% in the SFOD vs CD and SOD groups respectively; P<0.05). The epididymal ω6:ω3 ratio was higher in the SFOD versus CD and SOD groups, whereas the unsaturation index was higher in the SOD and SFOD groups than in CD group. Sperm membrane integrity was diminished in both the SOD and SFOD groups, but there was no difference in sperm reactive oxygen species production in these two groups compared with the CD group. The fertilisation rate was lower in the SFOD compared with the CD and SOD groups. In conclusion, although both HFDs affected sperm quality, the fertilising ability was more altered by the excessive dietary ω6:ω3 ratio than by the net ω6 content.

Altered human voice processing in the frontal cortex and a developmental language delay in 3- to 5-year-old children with autism spectrum disorder

The inferior frontal and superior temporal areas in the left hemisphere are crucial for human language processing. In the present study, we investigated the magnetic mismatch field (MMF) evoked by voice stimuli in 3- to 5-year-old typically developing (TD) children and children with autism spectrum disorder (ASD) using child-customized magnetoencephalography (MEG). The children with ASD exhibited significantly decreased activation in the left superior temporal gyrus compared with the TD children for the MMF amplitude. If we classified the children with ASD according to the presence of a speech onset delay (ASD - SOD and ASD - NoSOD, respectively) and compared them with the TD children, both ASD groups exhibited decreased activation in the left superior temporal gyrus compared with the TD children. In contrast, the ASD - SOD group exhibited increased activity in the left frontal cortex (i.e., pars orbitalis) compared with the other groups. For all children with ASD, there was a significant negative correlation between the MMF amplitude in the left pars orbitalis and language performance. This investigation is the first to show a significant difference in two distinct MMF regions in ASD – SOD children compared with TD children.

TNFR/TNF-α signaling pathway regulates apoptosis of alveolar macrophages in coal workers' pneumoconiosis.

We explored the role of TNFR/TNF-α signalingin apoptosis among alveolar macrophages (AM) and its relevance to the development of coal workers’ pneumoconiosis (CWP). Purified alveolar macrophages (AMs) were prepared from bronchoalveolar lavage fluid harvested from 366 CWP patients and 120 healthy subjects enrolled inthe study. The purified AMs were then divided into control, SOD, anti-TNFR, TNFR and NFkB inhibitor groups and analyzed for apoptosis usingflow cytometry (sub-diploid peak) and western blotting (Bcl-2, Caspase-3 and Caspase-8 expression). We found thatAM apoptosis washigher amongCWP patients than thehealthycontrols. Expression ofBcl-2, Caspase-3 and Caspase-8 was higher inAMs from CWP patientsthan in those from the controlsand correlated with increased AM apoptosis. Univariate and multivariate analyses suggested that CWP grade, initial exposure time, exposure time inyears, and CWP onset agewereall associated with altered levels of Bcl-2, Caspase-3 and Caspase-8. Inhibition of TNFR/TNF-α signaling usinganti-TNFR antibody, SOD or NFkB inhibitionreduced AM apoptosisand decreased Bcl-2, Caspase-3 and Caspase-8 expression. These data suggestinhibition of a TNFR/TNF-α signaling pathway is a potentiallyeffective means ofalleviating CWP by inhibiting AM apoptosis.

Oxidative Stress Markers in GnRH Agonist and Antagonist Protocols in IVF.

SummaryBackgroundOur aim was to study the effect of GnRH agonist and antagonist protocols of ovarian stimulation on oxidative stress parameters in serum and the influence of oxidative stress parameters change on the outcome of IVF cycles.MethodsThis prospective study included 82 patients who underwent IVF procedures. We determined SOD, MDA and SH groups in serum. Serum samples were obtained between the second and fourth day of the cycle and on the day of HCG administration during ovarian stimulation.ResultsPatients were divided into two groups depending on the protocol of stimulation. The mean total and mature oocytes number and number of fertilized oocytes were higher in GnRH agonist group. There was no significant difference in biochemical pregnancy, miscarriage and live–birth rate in both groups. Mean serum SOD was significantly lower, while mean serum MDA and SH groups were significantly higher after ovarian stimulation. Delivery rate was higher in patients without OS while miscarriage rate was higher in patients with OS.ConclusionsOur study confirmed that there is a difference in the concentration of oxidative stress parameters before and after ovarian stimulation. IVF outcome is better in patients without OS after ovarian stimulation. However, the protocol of ovarian stimulation is neither associated with a change in oxidative stress parameters nor with the outcome of ART procedures.

The role of superoxide dismutase on pregnancy rates of women undergoing intrauterine insemination

Background: Recently, assisted reproductive technology has been widely accepted as a well-established procedure to treat infertility. Unfortunately, the success rate is still below expectations, whereas the rate of infertility has risen steadily. Intrauterine insemination procedure is the highest performed compared to other procedures, but the success rate is still lowest, around 10%. One cause of failure has been thought to occur due to lack of oocytes and embryos protection from cleaning of oxygen radicals. Reactive oxygen species negatively affect the maturation and interaction of gamete, fertilization, as well as acceleration of pathological states of the reproductive tract. On female reproduction, an antioxidant is essential for the maturation and quality of oocyte, implantation, placentation, fetal growth, and development of the organ. Concentrations of antioxidants have an important role in the reproduction, and interventions to reduce the influence of reactive oxygen species can improve the quality of embryos and their implantation.Objective: To determine the effect of antioxidants (superoxide dismutase [SOD]) in ovarian stimulation response and pregnancy rates in women undergoing intrauterine insemination.Material: Women undergoing intrauterine insemination procedures and who met the criteria of research were randomly and in double-blind divided into two groups, that is, SOD group and placebo group. During ovarian stimulation, 20 patients randomly selected were administered capsules containing 250 mg SOD (glisodin®, Kalbe Pharmaceutical, tbk.) twice a day, and the remaining 20 patients were given the same dose and color capsules containing placebo. Furthermore, ovarian activity and endometrial thickness were monitored by regular transvaginal ultrasound scans. A dose of 5000 U of hCG (Profasi, Serono Pharmaceuticals Ltd., UK) was administered when the leading cohort of follicles reached a diameter of 18–22 mm. Intrauterine insemination with prepared sperms was then performed 34–36 hours following hCG using Friedman catheter. Biochemical pregnancy is determined at day 15 after insemination with the cut-off point 25I U/mL. The data are compared between two groups and analyzed using SPSS 16 with a 95% confidence interval.Result: Forty women underwent intrauterine insemination cycles, with 20 cycles administered to each group, SOD and placebo. There were five (12.5%) positives for chemical pregnancies, four (80%) in SOD, and one (20%) in placebo. The pregnancy rates tend to be higher in the SOD group than in the placebo group, even though it was not significantly different (p = 0.151). The ovarian stimulation determined by the number of growing follicles and endometrial thickness showed that more follicles (≥ 6 follicles) were found in SOD group (n = 10, 58.8%) than in placebo (n = 7, 41.2%); however, it was not statistically significant (p = 0.075). Endometrial thickness of 10 mm or more is statistically significant (p = 0.017), which is higher (79.2%) in SOD compared with placebo (20.8%) and yields significantly (p = 0.0251) greater pregnancy rates (n = 4, 80%) than placebo (n = 1, 20%).Conclusions: SOD provides better stimulation response and increased pregnancy rates in intrauterine insemination program.

Effects of Antioxidant on Reduction of Hindlimb Muscle Atrophy Induced by Cisplatin in Rats

The purpose of this study was to examine the effects of Cu/Zn SOD on reduction of hindlimb muscular atrophy induced by cisplatin in rats. Forty-two rats were assigned to three groups; control group, Cisplatin (CDDP) group and cisplatin with Cu/Zn SOD (CDDP-SOD) group. At day 35 hindlimb muscles were dissected. Food intake, activity, withdrawal threshold, muscle weight, and Type I, II fiber cross-sectional area (CSA) of dissected muscles were measured. Relative SOD activity and expression of MHC and phosphorylated Akt, ERK were measured after dissection. Muscle weight and Type I, II fiber CSA of hindlimb muscles in the CDDP group were significantly less than the control group. Muscle weight and Type I, II fiber CSA of hindlimb muscles, food intake, activity, and withdrawal thresholds of the CDDP-SOD group were significantly greater than the CDDP group. There were no significant differences in relative SOD activities of hindlimb muscles between the CDDP-SOD and CDDP groups. MHC expression and phosphorylated Akt, ERK of hindlimb muscles in the CDDP-SOD group were significantly greater than the CDDP group. Cu/Zn SOD attenuates hindlimb muscular atrophy induced by cisplatin through increased food intake and activity. Increment of phosphorylated Akt, ERK may relate to attenuation of hindlimb muscular atrophy.

Mo1487 Inicial Precut vs. an Algorithmic Approach Using a Wire-Guided Sphincterotome for Selective Biliary Cannulation

post-ERCP acute pancreatitis in each of the SOD groups. The following data were recorded: age, sex, indication for SOM, basal SOM results, bile duct diameter, endoscopic sphincterotomy, and number of patients in whom pancreatitis developed. Pancreatitis was defined as abdominal pain combined with an increase in serum amylase and/or lipase 3 times the upper limit of normal within 24 hours of the procedure. . Factors associated with acute pancreatitis in each group were examined by univariate analysis (UA). Significant variables by UA were included in a stepwise logistic regression analysis to determine independent predictors of acute pancreatitis. Statistic significance was determined at a p 0.05. Results: We identified 147 patients with SOD (86% females, mean age 42 1.1(SE) years). The indications for SOM were unexplained pancreatobiliary pain and a history of acute recurrent pancreatitis. Patients were classified as SOD 1 in 16, SOD 2 in 66 and SOD 3 in 65 cases. Biliary sphincterotomy was performed in all patients and pancreatic sphincterotomy was performed in 68/147 (46%) patients. 101 (69%) patients underwent stenting of the pancreatic duct. Post-ERCP pancreatitis occurred in 27% of the study cohort. Patients with SOD 3 had a higher frequency of postERCP pancreatitis compared to the SOD 2 and SOD 1 group (37% vs. 21% vs. 13% respectively). Multiple logistic regression analysis revealed that the type of SOD was then strongest predictor of pancreatitis with SOD 3 having the greater frequency of post-ERCP pancreatitis (OR 7.68, p 0.05) compared to those with SOD 1. There was no significant difference in post-ERCP pancreatitis between SOD 2 and SOD 1 (p 0.21). Age, sex, absence of a pancreatic stent, and a pancreatic sphincterotomy were not independently associated with post-ECRP pancreatitis. Conclusions: SOD 3 is strongly associated with the development of post-ERCP pancreatitis compared to SOD 1 and SOD 2. SOD 3 may have a different pathogenesis than SOD 1 and 2, thus placing these patients at a higher risk for this complication.

Exogenous Superoxide Dismutase: Action on Liver Oxidative Stress in Animals with Streptozotocin-Induced Diabetes

Aim. To investigate the effects of exogenous antioxidant copper zinc superoxide dismutase (Cu/Zn SOD) on oxidative stress in the experimental model of diabetes mellitus (DM). Methods. Twenty eight male Wistar rats divided in four groups were used: control (CO), controls treated with SOD (CO + SOD), diabetics (DM), and diabetics treated with SOD (DM + SOD). SOD (orgotein, 13 mg/Kg body weight was administered. DM was induced by a single streptozotocin injection (i.p., 70 mg/kg), and 60 days later, we evaluated liver oxidative stress. Results. Liver lipoperoxidation was increased in the DM group and significantly decreased in the DM + SOD group. Nitrite and nitrate measures were reduced in the DM and increased in the DM + SOD group, while iNOS expression in the DM group was 32% greater than in the CO and 53% greater in the DM + SOD group than in the DM group (P < .01). P65 expression was 37% higher in the DM (P < .05), and there was no significant difference between the DM and DM + SOD groups. Conclusion. SOD treatment reduced liver oxidative stress in diabetic animals, even though it did not change NFκB. SOD also increased NO, probably by the increased dismutation of the superoxide radical. The iNOS expression increase, which became even more evident after SOD administration.

Formation of Hydrogen Peroxide and Reduction of Peroxynitrite via Dismutation of Superoxide at Reperfusion Enhances Myocardial Blood Flow and Oxygen Consumption in Postischemic Mouse Heart

Reactive oxygen/nitrogen species suppress myocardial oxygen consumption. In this study, we determined that endogenous hydrogen peroxide through dismutation of superoxide enhances postischemic myocardial blood perfusion and oxygen consumption. Electron paramagnetic resonance oximetry was applied to monitor in vivo tissue Po2 in mouse heart subjected to regional ischemia reperfusion. Heart rate, arterial blood pressure, blood flow, infarction, and activities of mitochondrial NADH dehydrogenase and cytochrome c oxidase were measured in six groups of wild-type (WT) and endothelial nitricoxide synthase knock-out (eNOS(-/-)) mice treated with phosphate-buffered saline (PBS), superoxide dismutase mimetic (SOD(m)) M40403 [a manganese(II)-bis(cyclohexylpyridine)-substituted macrocyclic superoxide dismutase mimetic, C21H35Cl2MnN5], 10006329 EUK 134 [EUK134, manganese 3-methoxy N,N(1)-bis(salicyclidene)ethylenediamine chloride], and SOD(m) plus glibenclamide to study the protective effect of hydrogen peroxide via dismutation of superoxide on the activation of sarcolemmal potassium channels. In the PBS group, there was an overshoot of tissue Po2 after reperfusion. Treatment with SOD(m), EUK134, and SOD(m) + glibenclamide protected mitochondrial enzyme activities, reduced infarct size, and suppressed the postischemic hyperoxygenation. In particular, in the SOD(m)-treated group, there was a transient peak of tissue Po2 at 9 min after reperfusion, which was dependent on endogenous hydrogen peroxide but not nitric oxide formation as it appeared in both WT and eNOS(-/-) mice. Blood flow and rate pressure product were higher in the SOD(m) group than in other groups, which contributed to the transient oxygen peak. Thus, SOD mimetics protected mouse heart from superoxide-induced reperfusion injury. With treatment of different SOD mimetics, it is concluded that endogenous hydrogen peroxide via dismutation of superoxide at reperfusion enhances postischemic myocardial blood perfusion and mitochondrial oxygen consumption, possibly through activation of sarcolemmal ATP-sensitive potassium channels.

Empirical study of the transportation of pulmonary surfactant-super oxide dismutase liposome to lung tissue in rats

To study the feasibility of the transportation of pulmonary surfactant-super oxide dismutase (PS-SOD) liposome to lung tissue in rats. 32 Wistar rats were randomly divided into 4 groups (8 rats in each group): normal saline group, PS group, SOD group, PS-SOD liposome group. Each group was further divided into two groups (4 rats in each group), and the rats were respectively killed 2 and 24 hours after the operation. While the biological activity of SOD in irrigating solution and tissue homogenate were detected, lung tissue were labeled with fluorescent and then observed under microscope and transmission electron microscope. PS-SOD liposome was corps rounds with monolayer lipid with stable surface tension and antioxidative activity. At the point of 2 hours after operation, while the SOD biological activity of irrigating solution in PS-SOD liposome group (32.87 +/- 5.47) and SOD group (33.14 +/- 5.61) were obviously higher than that in normal saline group (2.15 +/- 0.17, P < 0.01), there was no difference between them (P > 0.05). The mean fluorescence optical density in PS-SOD liposome group (0.109 +/- 0.018) was lower than that in normal saline group (0.144 +/- 0.052) and PS group (0.143 +/- 0.026, P < 0.01). 24 hours after operation, the SOD biological activity of irrigating solution in PS-SOD liposome group (11.54 +/- 1.42) was the highest (P < 0.01) and the mean fluorescence optical density in PS-SOD liposome group (0.112 +/- 0.018) was the lowest (P < 0.01). The SOD biological activity of tissue homogenate in PS-SOD liposome group (2 h: 16.83 +/- 2.69, 24 h: 15.70 +/- 2.75) was higher than that in normal saline group (2 h: 5.79 +/- 0.93, 24 h: 5.84 +/- 1.31) and in SOD group (2 h: 7.07 +/- 1.04, 24 h: 6.11 +/- 1.06, P < 0.01) both at the point of 2 and 24 hours after the operation. Lots of PS-SOD liposome was observed in type II alveolar epithelial cells under transmission electron microscope. Intrathecal administ ration of PS-SOD liposome enhanced the transportation of SOD into lung tissue and its antioxidative activity.