Abstract
SummaryBackgroundOur aim was to study the effect of GnRH agonist and antagonist protocols of ovarian stimulation on oxidative stress parameters in serum and the influence of oxidative stress parameters change on the outcome of IVF cycles.MethodsThis prospective study included 82 patients who underwent IVF procedures. We determined SOD, MDA and SH groups in serum. Serum samples were obtained between the second and fourth day of the cycle and on the day of HCG administration during ovarian stimulation.ResultsPatients were divided into two groups depending on the protocol of stimulation. The mean total and mature oocytes number and number of fertilized oocytes were higher in GnRH agonist group. There was no significant difference in biochemical pregnancy, miscarriage and live–birth rate in both groups. Mean serum SOD was significantly lower, while mean serum MDA and SH groups were significantly higher after ovarian stimulation. Delivery rate was higher in patients without OS while miscarriage rate was higher in patients with OS.ConclusionsOur study confirmed that there is a difference in the concentration of oxidative stress parameters before and after ovarian stimulation. IVF outcome is better in patients without OS after ovarian stimulation. However, the protocol of ovarian stimulation is neither associated with a change in oxidative stress parameters nor with the outcome of ART procedures.
Highlights
The outcome of ART procedures is affected by several factors including the protocol of ovarian stimulation and oxidative stress
Our aim was to study the effect of GnRH agonist and antagonist protocols of ovarian stimulation on oxidative stress parameters in serum and the influence of oxidative stress parameters change on the outcome of IVF cycles
The mean total and mature oocytes number and number of fertilized oocytes were higher in GnRH agonist group
Summary
The outcome of ART procedures is affected by several factors including the protocol of ovarian stimulation and oxidative stress. Controlled ovarian stimulation is part of assisted reproductive procedures and several protocols are designed with the combination of GnRH agonists and antagonists and gonadotropins. Long-acting GnRH agonists were introduced in the late 1980s to ovarian stimulation in ART to downregulate endogenous pituitary gonadotropin secretion and prevent premature LH surge during exogenous gonadotropin stimulation. In the typical »long protocol«, GnRH agonist application begins in the midluteal phase with an acute reaction and release of stored pituitary gonadotropins in the response known as »Flare effect«, and suppresses endogenous gonadotropin secretion during the 10 days and longer while occupying the receptor. Contrary to the long-acting agonists, the GnRH antagonists block the GnRH receptor in a competitive, dose-dependent manner, and with no flare effect. The duration of stimulation is shorter, and the total dose of exogenous gonadotropins is decreased [2]
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