Snail Theba Pisana Research Articles

Effect of Eucalyptus globulus oil and Ricinus communis methanolic extract as potential natural molluscicides on the reproductive biology and some antioxidant enzymes of the land snail, Theba pisana

This study was conducted to investigate the effects of Eucalyptus globulus oil and methanolic extract of Ricinus communis seeds on the reproductive biology of the land snail Theba pisana. For this purpose, the snails were exposed to different concentrations of these plant extracts for six weeks. Rates of oviposition, hatching percentages, reproductive hormones and the histological structures of the hermaphrodite gland were estimated. Antioxidant enzymes were also estimated. The obtained results revealed that all tested concentrations of both tested natural products exerted hazardous effects on exposed snails. The mean egg number/snail treated with 2% E. globulus and R. communis were significantly decreased to 22 and 14 eggs respectively compared to 79 eggs for control. The hatching rates were dramatically decreased with increasing concentrations of both products. 2% of R. communis extract caused highly significant decrease in the activities of CAT, SOD, GST and MDA antioxidant enzymes while the same concentration of Eucalyptus oil resulted in elevations of CAT and SOD activities and significantly decreased GST and MDA activities. Levels of reproductive hormones were greatly disrupted and the histological structures of hermaphrodite acini as well as various developmental stages of spermatogenesis and oogenesis of treated snails were strictly spoiled.

Noxiousness Valuation of Two Pharmaceutical Plants on White Garden Snail and Their Side Influences on Biochemical Parameters and Histopathological Changes in Brown Rat

The current study aimed to evaluated the toxicity of Abrus precatorius (AP) and Ricinus communis (RC) plants on land snail (Theba pisana) and their effects on biochemical and histological changes in brown rat (Rattus norvegicus). Different concentration (1%, 1.5%, 2% and 2.5%) of AP and RC were used in this study to evluate their effects. The findings of this study pertained that the seeds of AP and RC had potential impact to control T. pisana and the LC50 of AP and RC had negative effects on many life cycle stages of T. pisana. The oviposition and new generation of T. pisana decreased by 81.2% and 62.5% and by 100% and 96.7%, while the infertility rate declined by 96.4% and 82.4% after treating T. pisana by AP and RC, respectively. Results also showed that AP and RC had weak influences on the liver, spleen, intestine, kidney and testis tissues of R. norvegicus. Furthermore, the effect was relatively higher for A. precatorius than R. communis on R. norvegicus males.

Multiple Introductions of the Pestiferous Land Snail Theba pisana (Müller, 1774) (Gastropoda: Helicidae) in Southern California.

Simple SummaryIn Southern California, USA, the introduced white Italian land snail, Theba pisana, is prolific and locally pestiferous. To better understand its diversity and infer its parent population(s), we collected it from Los Angeles and San Diego counties and generated and analyzed gene sequence data (CO1, 16S, ITS2) that we compared between localities and to T. pisana CO1 barcodes from around the world. We also compared the morphology of the jaw, radula, and reproductive systems in T. pisana from Los Angeles and San Diego Counties. We found that T. pisana living at several sites in Los Angeles County in 2019–2020 had a single origin and were most similar in CO1 DNA sequence, based on available data, to specimens from Malta. Theba pisana collected from one site in San Diego County differed from Los Angeles T. pisana and were most similar in CO1 barcode sequence to specimens from Morocco. Jaw and mucous gland morphology also differed between Los Angeles and San Diego populations, but it is unclear if these traits are unique to lineages of T. pisana or if they change during a snail’s lifetime. We discuss how Los Angeles and San Diego T. pisana lineages may have arrived in Southern California and anticipate that the genetic data and morphological observations generated by this study will inform future studies of T. pisana where it is native and introduced.The terrestrial land snail Theba pisana is circum-Mediterranean in native range and widely introduced and pestiferous in regions around the world. In California, USA, T. pisana has been recorded intermittently since 1914, but its source population(s) are unknown, and no morphological or molecular analyses within or between California populations have been published. Therefore, we compared molecular data (CO1, 16S, ITS2) and internal morphology (jaw, radula, reproductive system) in T. pisana collected from Los Angeles and San Diego counties in 2019–2020. DNA barcode (CO1 mtDNA) analysis revealed that T. pisana from Los Angeles County was most similar to T. pisana from the Mediterranean island of Malta, and northern San Diego County-collected specimens were most similar to T. pisana from Morocco. Morphology of the jaw and mucous glands also differed between Los Angeles and San Diego populations, but it is unclear if traits are lineage-specific or artifacts of ontogeny. Several pathways of introduction into Southern California are possible for this species, but evidence for intentional vs. accidental introduction of present populations is lacking. Subsequent investigation(s) could use the data generated herein to assess the provenance of T. pisana elsewhere in California and/or worldwide and inform analyses of reproductive biology and systematics in this widespread species.

Contact toxicity and biochemical impact of metaldehyde against the white garden snail Theba pisana (Müller, 1774).

Terrestrial snails are one of the most damaging threats to sustainable agriculture. Chemical control using molluscicides is the main approach used to combat these agricultural pests. Metaldehyde is the active ingredient in most snail control products in use. However, its toxicity indices and mode of action have scarcely been investigated. For the first time, we characterized the metaldehyde contact toxicity indices against the white garden snail Theba pisana. The biochemical impact of metaldehyde on acetylcholinesterase (AChE), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), alkaline phosphatase (ALP) and glutathione S-transferase (GST) activities and the lipid peroxidation (LPO) level was investigated. The median lethal dose (LD50 ) values at 24, 48 and 72 h of treatment were 11.33, 8.53, and 6.87 μg g-1 body weight (BW), respectively; while, the median lethal time (LT50 ) values were 88.16, 55.85, and 25.67 h when doses of 6, 8, and 12 μg g-1 BW were applied, respectively. In the snails treated with 2.83 and 5.67 μg g-1 BW (¼ and ½ LD50 at 24 h of treatment) and 2.13 and 4.27 μg g-1 BW (¼ and ½ LD50 at 48 h of treatment), higher AChE, GST, AST, ALT, and ALP activities as well as higher levels of LPO were observed compared with that of untreated snails. Metaldehyde displayed dose- and time-dependent contact toxicity. The biochemical results suggest that metaldehyde may have neurotoxic and cytotoxic actions in terrestrial snails. Application of metaldehyde in ways that could control pest snails and slugs and reduce its negative impact on the environment are discussed. © 2021 Society of Chemical Industry.

Phenotypic plasticity of invasive Carpobrotus edulis modulates tolerance against herbivores

The anthropogenic movement of species has favoured the introduction of invasive plants worldwide. Invasive plants are frequently released from their natural enemies; however, new associations with generalist herbivores may induce defence mechanisms of non-native plants. Defensive traits are often directly related to the highly competitive ability, but also to potential antagonisms and mutualisms that they can establish with soil microorganisms. Here, we examined whether the intraspecific competition and soil microorganisms influence the morphological and physiological traits of Carpobrotus edulis when is being attacked by the native generalist snail Theba pisana. To achieve this, we grew two C. edulis individuals in separate and same pots filled with live or sterile sand, and with or without T. pisana. Our results indicated that herbivory induced an increase of shoot biomass in attacked C. edulis individuals (i.e., treated donor plants), as well as in un-attacked neighbouring individuals co-growing in the same pot (i.e., untreated recipient plants). Nevertheless, intraspecific competition nor soil microorganisms did not affect the growth of C. edulis despite reduced physiological activity and damage caused by the herbivore. Overall, our findings revealed that C. edulis individuals tolerate snail attack by inducing a compensatory growth response. We conclude that phenotypic plasticity of invasive C. edulis favours tolerance against herbivores, but we also suggest that plant-plant interactions probably determine the plant growth of un-attacked neighbouring C. edulis individuals, thus favouring their invasion mechanisms.

Evaluation of Kz Mineral Oil for Molluscicidal and Biochemical Activities against White Garden Snail Theba pisana (Müller) as a Safe Alternative to Pesticides and Honey Bee Apis mellifera L. Friendly Compound

Evaluation of Kz Mineral Oil for Molluscicidal and Biochemical Activities against White Garden Snail Theba pisana (Müller) as a Safe Alternative to Pesticides and Honey Bee Apis mellifera L. Friendly Compound

Mortality of the invasive white garden snail Theba pisana exposed to three US isolates of Phasmarhabditis spp (P. hermaphrodita, P. californica, and P. papillosa).

Theba pisana is a serious snail pest in many parts of the world and affects diverse crops including grain, vegetables, grapevines, and ornamental plants and shrubs. Due to its gregarious nature, ability to reproduce rapidly, and the difficulty of controlling it by conventional methods, it has the potential to become a significant pest where introduced. Mitigating this pest is an important challenge that must be addressed. Phasmarhabditis hermaphrodita, is a gastropod-killing nematode that is commercially available only in Europe (Nemaslug ®) and Sub-Saharan Africa (Slugtech ® SP). The use of effective gastropod-killing nematodes in the genus Phasmarhabditis (P. hermaphrodita, P. californica and P. papillosa) in California may provide one strategy for alleviating the potential damage and further spread of these snails, which are currently limited to San Diego and Los Angeles counties. Laboratory assays demonstrated for the first time that US isolates of P. hermaphrodita, P. californica and P. papillosa at 150 DJs/cm2 caused significant mortality and are equally lethal to T. pisana. Molluscicidal efficacy of these nematodes are comparable with those of iron phosphate, at the recommended high dose of 4.88 kg/m2. Additional trials are needed to determine their effects at lower dose and whether they are dependent on the size or age of the snails.

The mitochondrial genome of the land snail Theba pisana (Müller, 1774) (Stylommatophora: Helicidae): the first complete sequence in the genus Theba

The complete mitochondrial genome of the white garden snail Theba pisana (Müller, 1774) has been sequenced and annotated in this study. The entire circular genome is 14,795 bp in size and represents the first mitochondrial genome in the genus Theba, with two ribosomal RNA genes, 22 transfer RNA genes, 13 protein coding genes. All of genes are divided into two groups, including 24 genes on the majority coding strand (J strand) and others on the minority coding strand (N strand).The phylogeny supports the relationship of species in Helicidae.

Herbivory induced non-local responses of the clonal invader Carpobrotus edulis are not mediated by clonal integration

The anthropogenic displacement of species around the world results in new environmental situations where native and exotic species coexist. Exotic plants have to face native herbivores, and interactions between introduced plants and native herbivores seem to play an important role in the invasiveness of some exotic plant species. We studied the role of clonal integration in induce morphological, physiological, and biochemical responses in the clonal invader Carpobrotus edulis against the attack of the native snail Theba pisana. Our results demonstrated the presence of labour division mediated by physiological integration, with a significant increase of photosynthesis potential (both at morphological and physiological) in un-attacked integrated ramets. This response could be especially important under herbivory, as the negative impact of T. pisana on the photosynthetic structures of attacked C. edulis ramets could be buffered by transferring the dependence of photosynthetic activity to the un-attacked ramets. Our results also showed a constitutive resistance in un-attacked apical ramets, showing a similar amount of defence compounds to those exhibited in the basal branches attacked by snails. Results reported a non-local compensatory response, which there was an increase of total biomass in apical ramets when their basal ramets were attacked by the herbivore. We interpret this result as a compensatory response, with these apical ramets increasing shoot biomass to compensate for the biomass loss due to a potential attack from herbivores. However, this non-local response was not mediated by physiological integration but probably due to belowground communication, with the presence of alarm signals released by root exudates. We conclude that the attack of this snail is not enough to be a possible biological control due to the compensatory response to this snail by C. edulis, favouring their expansion. Future studies should focus on unravelling the role of belowground communication in the defensive responses of C. edulis.

Early evidence for a virus-like agent infecting the pest snail Theba pisana (Gastropoda: Pulmonata) in Southern Italy.

The Mediterranean land snail Theba pisana (Mollusca: Helicidae) is an introduced agricultural pest in many countries around the world, including Australia, Israel, USA and South Africa. In addition, this snail is an intermediate host of parasites of importance in both human and veterinary medicine. In this study, a natural population of T. pisana snails on the Domitian coast of Italy was surveyed following a mass mortality event. By light microscopy, 30% of the collected individuals showed in the calcium cells of the digestive gland the presence of hypertrophied nuclei containing eosinophilic to weakly basophilic inclusion bodies. Ultrastructural examination by transmission electron microscopy (TEM) showed nuclear inclusions constituted by a reticulated stroma into which unenveloped, roundish virus-like particles (38±4nm in diameter) were present. To the best of our knowledge this could be the first evidence for a virus-like agent infecting the gastropod T. pisana, which may open new biocontrol perspectives of the this pest worldwide.

Global metabolite analysis of the land snail Theba pisana hemolymph during active and aestivated states

The state of metabolic dormancy has fascinated people for hundreds of years, leading to research exploring the identity of natural molecular components that may induce and maintain this state. Many animals lower their metabolism in response to high temperatures and/or arid conditions, a phenomenon called aestivation. The biological significance for this is clear; by strongly suppressing metabolic rate to low levels, animals minimize their exposure to stressful conditions. Understanding blood or hemolymph metabolite changes that occur between active and aestivated animals can provide valuable insights relating to those molecular components that regulate hypometabolism in animals, and how they afford adaptation to their different environmental conditions. In this study, we have investigated the hemolymph metabolite composition from the land snail Theba pisana, a remarkably resilient mollusc that displays an annual aestivation period. Using LC-MS-based metabolomics analysis, we have identified those hemolymph metabolites that show significant changes in relative abundance between active and aestivated states. We show that certain metabolites, including some phospholipids [e.g. LysoPC(14:0)], and amino acids such as l-arginine and l-tyrosine, are present at high levels within aestivated snails. Further investigation of our T. pisana RNA-sequencing data elucidated the entire repertoire of phospholipid-synthesis genes in the snail digestive gland, as a precursor towards future comparative investigation between the genetic components of aestivating and non-aestivating species. In summary, we have identified a large number of metabolites that are elevated in the hemolymph of aestivating snails, supporting their role in protecting against heat or desiccation.

Multi-tissue transcriptomics for construction of a comprehensive gene resource for the terrestrial snail Theba pisana.

The land snail Theba pisana is native to the Mediterranean region but has become one of the most abundant invasive species worldwide. Here, we present three transcriptomes of this agriculture pest derived from three tissues: the central nervous system, hepatopancreas (digestive gland), and foot muscle. Sequencing of the three tissues produced 339,479,092 high quality reads and a global de novo assembly generated a total of 250,848 unique transcripts (unigenes). BLAST analysis mapped 52,590 unigenes to NCBI non-redundant protein databases and further functional analysis annotated 21,849 unigenes with gene ontology. We report that T. pisana transcripts have representatives in all functional classes and a comparison of differentially expressed transcripts amongst all three tissues demonstrates enormous differences in their potential metabolic activities. The genes differentially expressed include those with sequence similarity to those genes associated with multiple bacterial diseases and neurological diseases. To provide a valuable resource that will assist functional genomics study, we have implemented a user-friendly web interface, ThebaDB (http://thebadb.bioinfo-minzhao.org/). This online database allows for complex text queries, sequence searches, and data browsing by enriched functional terms and KEGG mapping.

COMPARATIVE MOLLUSCICIDAL ACTIVITY OF ABAMECTIN AND METHOMYL AGAINST Eobania vermiculata (Müller) AND Theba pisana (Müller

The molluscicidal activity of abamectin were assessed compared with methomyl against the most two abundant snail species brown garden snail (BGS) Eobania vermiculata and white garden snail (WGS) Theba pisana under laboratory conditions. The results indicated that LC50 values of methomyl were almost equal LC50 values of abamectin indicating more molluscicidal potency of abamectin and methomyl. Results also revealed a complete consistency of correlation between the LC50 values and exposed time intervals. The high molluscicidal activity of abamectin against E. vermiculata and T. pisana compared to methomyl using topical application technique could be used practically in the field of land snails control program and overcome some drawbacks of using bait forms in this respect. Comparing the LD50 values indicate the significant variation of the molluscicidal activity between the two applications methods used in addition to the variation between the two species of chosen snails, the brown garden snail E. vermiculata were resistant species than the white garden snail T. pisana. The molluscicidal activity of abamectin were assessed compared with methomyl against the most two abundant snail species brown garden snail (BGS) Eobania vermiculata and white garden snail (WGS) Theba pisana under laboratory conditions. The results indicated that LC50 values of methomyl were almost equal LC50 values of abamectin indicating more molluscicidal potency of abamectin and methomyl. Results also revealed a complete consistency of correlation between the LC50 values and exposed time intervals. The high molluscicidal activity of abamectin against E. vermiculata and T. pisana compared to methomyl using topical application technique could be used practically in the field of land snails control program and overcome some drawbacks of using bait forms in this respect. Comparing the LD50 values indicate the significant variation of the molluscicidal activity between the two applications methods used in addition to the variation between the two species of chosen snails, the brown garden snail E. vermiculata were resistant species than the white garden snail T. pisana. Keywords: Molluscicides, Abamectin, Methomyl, Eobania vermiculata and Theba pisana

Geographic variation in thermal tolerance and strategies of heat shock protein expression in the land snail Theba pisana in relation to genetic structure.

Land snails are exposed to conditions of high ambient temperature and low humidity, and their survival depends on a suite of morphological, behavioral, physiological, and molecular adaptations to the specific microhabitat. We tested in six populations of the land snail Theba pisana whether adaptations to different habitats affect their ability to cope with thermal stress and their strategies of heat shock protein (HSP) expression. Levels of Hsp70 and Hsp90 in the foot tissue were measured in field-collected snails and after acclimation to laboratory conditions. Snails were also exposed to various temperatures (32 up to 54 °C) for 2 h and HSP messenger RNA (mRNA) levels were measured in the foot tissue and survival was determined. To test whether the physiological and molecular data are related to genetic parameters, we analyzed T. pisana populations using partial sequences of nuclear and mitochondrial DNA ribosomal RNA genes. We show that populations collected from warmer habitats were more thermotolerant and had higher constitutive levels of Hsp70 isoforms in the foot tissue. Quantitative real-time polymerase chain reaction (PCR) analysis indicated that hsp70 and hsp90 mRNA levels increased significantly in response to thermal stress, although the increase in hsp70 mRNA was larger compared to hsp90 and its induction continued up to higher temperatures. Generally, warm-adapted populations had higher temperatures of maximal induction of hsp70 mRNA synthesis and higher upper thermal limits to HSP mRNA synthesis. Our study suggests that Hsp70 in the foot tissue of T. pisana snails may have important roles in determining stress resistance, while Hsp90 is more likely implicated in signal transduction processes that are activated by stress. In the phylogenetic analysis, T. pisana haplotypes were principally divided into two major clades largely corresponding to the physiological ability to withstand stress, thus pointing to genetically fixed tolerance.

Differential peptide expression in the central nervous system of the land snail Theba pisana, between active and aestivated

Hypometabolism is a physiological state of dormancy entered by many animals in times of environmental stress. There are gaps in our understanding of the molecular components used by animals to achieve this metabolic state. The availability of genomic and transcriptome data can be useful to study the process of hypometabolism at the molecular level. In this study, we use the land snail Theba pisana to identify peptides that may be involved in the hypometabolic state known as aestivation. We found a total of 22 neuropeptides in the central nervous system (CNS) that were differentially produced during activity and aestivation based on mass spectral-based neuropeptidome analysis. Of these, 4 were upregulated in active animals and 18 were upregulated in aestivation. A neuropeptide known to regulate muscle contractions in a variety of molluscs, the small cardioactive peptide A (sCAPA), and a peptide of yet unknown function (termed Aestivation Associated Peptide 12) were chosen for further investigation using temporal and spatial expression analysis of the precursor gene and peptide. Both peptides share expression within regions of the CNS cerebral ganglia and suboesophageal ganglia. Relative transcript abundance suggests that regulation of peptide synthesis and secretion is post-transcriptional. In summary, we provide new insights into the molecular basis of the regulation of aestivation in land snails through CNS peptide control.

Bacterial and Viral Pathogens Associated with The White Garden Snail Theba Pisana (Müller)

The present study was performed during a periodical interval of nine months in (2014) at Alexandria Governorate, Egypt. Whereas, a laboratory trial was carried out to inspect the field collected dead adult land snails ofThebapisana (693 individuals). The inspection revealed that 19.0 % of these collected snails were infected with three pathogenic; bacteria; Bacillus theuringiensis (B.t.) (2.7%), B. cereus (0.1 %), and Brevibacterium sp. (10.1%) in addition to a polyhedrosis virus (2.7 %). Moreover, the made laboratory evaluation showed that the assessed daily cumulative mortality of snails as well as LC50 (Concentration of active ingredient responsible for killing 50% of the examined number animals) value of B.t post 13 days of snail treatment was (7.3x108 Visible spore (VS)/ml), while these determined values for Brevibacteriumsp . and polyhedrosis virus(P.V) were (7.6 x 108VSs/ml) and (3.3 x 106 Polyhedral Inclusion Bodies (P.I.Bs) /ml) after four and 16 days, respectively. The LT50 (Time in days, needed to kill 50% of the exposed pest) for Brevibacterium sp. ranged from 9.8 to 16.3 days for the different tested concentrations. The LT50 of lower concentration of polyhedrosis virus was much prolonged up to (10.6 days) compared to that of the highest concentration (1.5-day). Also, the LT50 values of B.t ranged from 10.2 to 17.5 day for the lowest and highest tested concentrations .The ease and simplicity of the followed bioassay procedures, and the reliability of the method described, as shown by the statistical evaluation of the obtained data, justify the use of the adopted method in the present study.

Plant selection and grazing activity of the invasive snail Theba pisana in coastal Algoa Bay, South Africa

The land snail Theba pisana is a coastal species native to the Mediterranean but has been introduced to regions all over the world, including South Africa and Australia, where it is considered a pest. This study examines the diet of T. pisana and its preference for certain dune plants in the Cape Recife Nature Reserve of Algoa Bay in the Eastern Cape, South Africa. Field observations identified plants being fed on by T. pisana, accounting for almost half of the dune plant species present in the study area. Five plant species, on which snails were found frequently and in high abundance, were selected for feeding preference experiments. Laboratory experiments and field observations indicate that T. pisana feed preferentially on Tetragonia decumbens over Osteospermum moniliferum, Osyris compressa and Cynanchum obtusifolium. It is clear that while T. pisana is reported to have a generalist diet, consuming a range of plant species commonly found in South African dune vegetation, it also exhibits a definite preference for T. decumbens. The reasons for this preference need to be investigated further.

Natural Variation in Resistance to Desiccation and Heat Shock Protein Expression in the Land SnailTheba pisanaalong a Climatic Gradient

Land snails frequently encounter desiccating conditions, and their survival depends on a suite of morphological, physiological, and molecular adaptations to the specific microhabitat. Strategies of survival can be determined by integrating information from various levels of biological organization. In this study, we used a combination of physiological parameters related to water economy and molecular factors (stress protein expression) to investigate the strategies of survival adopted by seven populations of the Mediterranean-type land snail Theba pisana from different habitats. We analyzed water compartmentalization during aestivation and used experimental desiccation to compare desiccation resistance. We also measured the endogenous levels of heat shock proteins (HSPs) Hsp72, Hsp74, and Hsp90 under nonstress conditions and analyzed the HSP response to desiccation in two populations that differed mostly in their resistance to desiccation. We revealed significant intraspecific differences in resistance to desiccation that seem to be determined by the speed of recruitment of the water-preserving mechanisms. The ability to cope with desiccating conditions was correlated with habitat temperature but not with the rainfall gradient, implying that in the coastal region, temperature is likely to have a major impact on desiccation resistance rather than precipitation. Also, higher desiccation resistance was correlated with higher constitutive levels of Hsp74 in the foot tissue. HSPs were upregulated during desiccation, but the response was delayed and was milder in the most resistant population compared to the most susceptible one. Our study suggests that T. pisana populations from warmer habitats were more resistant to desiccation and developed distinct strategies of HSP expression for survival, namely, the maintenance of high constitutive levels of Hsp70 together with a delayed and limited response to stress.

POPULATION DYNAMICS OF THE SNAIL THEBA PISANA (HELICIDAE) IN THE SAND DUNES OF NORTHERN ISRAEL

ABSTRACT The population dynamics of the land snail Theba pisana (Helicidae) was studied for two successive years in the sand dunes of northern Israel and under laboratory conditions. The field population in autumn consisted of two size classes: large snails of more than 10 mm in diameter and small snails of less than 5 mm in diameter. After the first rain, the large snails that emerged from aestivation laid eggs and died. After 3–4 weeks, a new cohort hatched and grew intensively throughout winter to the end of spring. However, a group of small snails ceased growing and entered an early aestivation. These growth-inhibited individuals retained immature size. Their proportion in the population is estimated at 5–10% throughout the year. The population in the laboratory showed a similar growth pattern and size distribution as that in the field. The inhibited-growth group in the laboratory was 40% of the population. We conclude that Theba pisana in Israel developed a strategy of mixed annual and biennial life ...

The snail Theba pisana as an indicator of soil contamination by trace elements: potential exposure for animals and humans

The ability of snails to accumulate trace elements is well known. We analysed the snail Theba pisana as an indicator of soil contamination by trace elements after a mine spill accident, to assess the exposure of animal and human consumption. Snails were collected in autumn and spring, when they are most active. In general, trace elements in the soft tissues reached greater concentrations in the contaminated soils than in the non-contaminated soils, although significant differences were only found for As, Cd, Cu Fe and Hg. Cadmium content in tissues, with a maximum value of 10 mg kg⁻¹ (dry matter), was the most worrying result. Trace element concentrations in the snail bodies were still of concern for human consumption; As and Cd concentrations were sometimes higher than the maximum concentration authorised in foodstuffs. Generally, nutritional status of the contaminated snails was not altered; concentrations of the main nutrients (Ca, K, Mg, P and S) were similar to those of the non-contaminated snails. Results reveal a potential risk for animal and human consumption of T. pisana. It seems thus advisable to avoid collecting this species for human consumption in the affected area. Periodic monitoring is recommended to assess the evolution of potential risk for animal consumption.