Abstract
The land snail Theba pisana is native to the Mediterranean region but has become one of the most abundant invasive species worldwide. Here, we present three transcriptomes of this agriculture pest derived from three tissues: the central nervous system, hepatopancreas (digestive gland), and foot muscle. Sequencing of the three tissues produced 339,479,092 high quality reads and a global de novo assembly generated a total of 250,848 unique transcripts (unigenes). BLAST analysis mapped 52,590 unigenes to NCBI non-redundant protein databases and further functional analysis annotated 21,849 unigenes with gene ontology. We report that T. pisana transcripts have representatives in all functional classes and a comparison of differentially expressed transcripts amongst all three tissues demonstrates enormous differences in their potential metabolic activities. The genes differentially expressed include those with sequence similarity to those genes associated with multiple bacterial diseases and neurological diseases. To provide a valuable resource that will assist functional genomics study, we have implemented a user-friendly web interface, ThebaDB (http://thebadb.bioinfo-minzhao.org/). This online database allows for complex text queries, sequence searches, and data browsing by enriched functional terms and KEGG mapping.
Highlights
The land snail Theba pisana is native to the Mediterranean region but has become one of the most abundant invasive species worldwide
We have provided a comprehensive neuropeptidome for T. pisana based largely on the transcriptome derived from three tissues: the central nervous system (CNS), hepatopancreas, and foot muscle[6]
Three normalized complimentary DNA (cDNA) libraries derived from RNA isolated from CNS, hepatopancreas, and foot muscle were constructed and sequenced using the pair-end Illumina platform
Summary
The land snail Theba pisana is native to the Mediterranean region but has become one of the most abundant invasive species worldwide. To provide a valuable resource that will assist functional genomics study, we have implemented a user-friendly web interface, ThebaDB (http://thebadb.bioinfo-minzhao.org/) This online database allows for complex text queries, sequence searches, and data browsing by enriched functional terms and KEGG mapping. Our transcriptome-based gene list maps the first genetic landscape for T. pisana, which advances our understanding on this invasive species towards targeted functional analyses. To share this valuable resource with the community to support further elucidation of molecular function, we www.nature.com/scientificreports/. Present all the sequence and annotation data online at http://thebadb.bioinfo-minzhao.org/ This user-friendly web portal may assist researchers to design potential pest control strategies at the systems biology level, such as gene-gene interaction
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