The loss of longitudinal connectivity in regulated rivers, both up- and downstream, has been detrimental for biodiversity worldwide. While progress has been made regarding upstream fish passage solutions, many questions remain unanswered regarding downstream passage alternatives. To address these knowledge gaps, we used Atlantic salmon (S. salar) smolts to experimentally assess the guidance efficiency and passage rates produced by several common screen-and-bypass fish guidance systems. Vertical screens with horizontally oriented bars extending across a turbine intake channel at a shallow angle (angled guidance screens), combined with a single, full-depth bypass entrance at their downstream end, were on average 20% more effective and produced passage rates that were 10 times higher than screens which extended perpendicularly across a turbine intake channel with vertically oriented bars that rose gradually towards the surface (inclined guidance screens) and with a bypass at the surface, on either side of the screen. Among inclined screens, gap width was negatively associated with guidance efficiencies and the smallest gap width (15 mm) exhibited a 41% greater guidance efficiency than the largest (30 mm). Among angled screens, performance was more closely linked to construction material as metal racks produced passage rates over three times faster than flexible Kevlar netting. Overall, passage through the guidance screens, and therefore into a tentative turbine intake area, was positively associated with gap width and was twice as prevalent among the inclined relative to angled guidance screens. Ultimately, an angled guidance screen with a 30 mm gap width produced the highest guidance efficiency and passage rates (a 30% improvement over the next best screen), while an inclined screen with a 30 mm gap width produced the lowest guidance efficiencies and passage rates. These results have implications for the suitability and performance of downstream fish passage solutions at both large- and small-scale hydropower plants where passage solutions are currently lacking or inadequate.