Robust environmental monitoring for Listeria monocytogenes often may not be feasible for small and very small meat processors in the United States due to the limitations in finances, staffing, or expertise. Three small/very small processors in Nebraska were sampled using sponge applicators in nonfood contact surface areas to determine if biofilm and sanitizer resistance behaviors of Pseudomonas could relate to the prevalence of L. monocytogenes and Listeria spp. in ready-to-eat meat processing environments. Samples were 3.3% (3/90) positive for L. monocytogenes, and 12.2% (11/90) of samples were positive for Listeria spp. Pseudomonas spp. were also isolated. When Listeria spp. and Pseudomonas spp. were assayed for biofilm production and resistance to a quaternary ammonia sanitizer, multiple isolates belonging to both genera capable of forming biofilms were identified. Four Pseudomonas spp. isolates resisted the 200 ppm manufacturer-recommended sanitizer concentration for food contact surface sanitation, and one Pseudomonas spp. isolated from a drain sample that was also positive for L. monocytogenes demonstrated a sanitizer minimum bactericidal concentration of 1000 ppm. These findings further support the need for monitoring of small and very small meat processors for L. monocytogenes as well as highlight the need to identify other bacteria in these processing environments, like Pseudomonas, that are resistant to environmental stressors.
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