Small Geographic Area Research Articles

Unexpected diversity and co-occurrence of phytotelmic frogs (Guibemantis) around Andasibe, one of the most intensively surveyed amphibian hotspots of Madagascar, and descriptions of three new species.

The area around the Malagasy village of Andasibe, which includes Analamazaotra-Mantadia National Park as well as other protected areas, is characterized by very species-rich and well-studied communities of animals and plants, but new species are still regularly discovered. Three species of phytotelmic frogs of the subgenus Pandanusicola in the genus Guibemantis are known from this area, G. flavobrunneus, G. liber, and G. pulcher. Further Pandanusicola frogs from this area have been provisionally assigned to G. bicalcaratus or G. albolineatus, pending detailed taxonomic review. During preliminary exploration of the ecology of these specialized frogs that live and reproduce in the leaf axils of Pandanus screw pines, we noticed the syntopic presence of two differently colored and differently sized Pandanusicola in Andasibe that could not be unambiguously assigned to any known species. A genetic screening revealed that these correspond to yet two further species in the area. Based on our data, seven species of Pandanusicola occur in Andasibe and nearby forests: (1) G. liber, the only non-phytotelmic species of the subgenus in the region; (2) G. flavobrunneus which is the largest species and characterized by a diagnostic yellowish brown dorsal pattern; (3) G. pulcher, characterized by translucent-green color with purplish brown spotting not observed in any other species in the area; (4) G. methueni, a brownish species usually lacking contrasted dorsolateral bands that differs from the other species in the area by emitting a characteristic trill-like advertisement call series (rather than clicks or chirps) and according to our data is widespread along Madagascars east coast; as well as three new species: (5) G. ambakoana sp. nov., a brownish species, typically with contrasted incomplete light dorsolateral bands and with single click-like advertisement calls; (6) G. vakoa sp. nov., a species that is equally brownish but lacks contrasted light dorsolateral bands and that has single click-like advertisement calls of very short duration; and (7) G. rianasoa sp. nov., a species that is smaller sized and has less distinct femoral glands than all the others, and emits a short series of soft chirp-like advertisement calls. All these species are genetically highly distinct, with >5% uncorrected pairwise distances in the mitochondrial 16S rRNA gene, and lack of haplotype sharing in two nuclear-encoded genes. The co-occurrence of seven Pandanusicola frogs in a relatively small geographic area is unprecedented in Madagascar and calls for in-depth studies of a possible differentiation in habitat use and life history.

Investigating strontium isotope linkage between biominerals (uroliths), drinking water and environmental matrices

This study presents the mineralogy and strontium isotope ratio (87Sr/86Sr) of 21 pathological biominerals (bladder and kidney stones) collected from patients admitted between 2018 and 2020 at the Department of Urology of the San Pio Hospital (Benevento, southern Italy). Urinary stones belong to the calcium oxalate, purine or calcium phosphate mineralogy types. Their corresponding 87Sr/86Sr range from 0.707607 for an uricite sample to 0.709970 for a weddellite one, and seem to be partly discriminated based on the mineralogy. The comparison with the isotope characteristics of 38 representative Italian bottled and tap drinking waters show a general overlap in 87Sr/86Sr with the biominerals. However, on a smaller geographic area (Campania Region), we observe small 87Sr/86Sr differences between the biominerals and local waters. This may be explained by external Sr inputs for example from agriculture practices, inhaled aerosols (i.e., particulate matter), animal manure and sewage, non-regional foods. Nevertheless, biominerals of patients that stated to drink and eat local water/wines and foods every day exhibited a narrower 87Sr/86Sr range roughly matching the typical isotope ratios of local geological materials and waters, as well as those of archaeological biominerals from the same area.Finally, we conclude that the strontium isotope signature of urinary stones may reflect that of the environmental matrices surrounding patients, but future investigations are recommended to ultimately establish the potential for pathological biominerals as reliable biomonitoring proxies, taking into the account the contribution of the external sources of Sr.

Optimized spatial information for 1990, 2000, and 2010 U.S. census microdata

We report on the successful completion of a project to upgrade the positional accuracy of every response to the 1990, 2000, and 2010 U.S. decennial censuses. The resulting data set, called Optimized Spatial Census Information Linked Across Time (OSCILAT), resides within the restricted-access data warehouse of the Federal Statistical Research Data Center (FSRDC) system where it is available for use with approval from the U.S. Census Bureau. OSCILAT greatly improves the accuracy and completeness of spatial information for older censuses conducted prior to major quality improvements undertaken by the Bureau. Our work enables more precise spatial and longitudinal analysis of census data and supports exact tabulations of census responses for arbitrary spatial units, including tabulating responses from 1990, 2000, and 2010 within 2020 block boundaries for precise measures of change over time for small geographic areas.

Do Bank Mergers has Forgone or Undergone Value? From the Perspective of Investor

Purpose: The purpose of the study is to explore banking industries investors’ perceptions about merged banks synergies “economies of scale, brand scope and market power “reaction to mergers.
 Design/methodology/approach: Based on the descriptive research design, a quantitative survey was adopted and administered to 117 investors through judgmental sampling techniques. Structural Equation Modeling (SEM) was then utilized to evaluate the significance of direct and indirect relationships between the various factors under study.
 Findings: The results confirm that the bank products & services and prices affect positively similarly, brand strategy and sales channels affects positively to the perceived gain of investors after mergers.
 Research limitations/implications: The absence of a longitudinal study measuring the post merger perception of investor is the main limitation of this study. Moreover, despite being insightful, the result of this study is generalized around small geographic area only.
 Originality/value: This study is an initial attempt to fill the void in published information about the behavioral finance of bank mergers. It provides new empirical insights about the effect of post-merger performance perceived by investor’s perspective.

Effect of landscape irrigation regulations on runoff volume from low-density residential catchments during two California droughts

The severe drought in California (2012–2016) generated significant public and government concern. State and local watering regulations were enacted to reduce residential and commercial water-use during the droughts. This study presents a comparison of residential runoff volumes before and after local landscape irrigation regulations were enacted during the droughts of 2008 and 2012–2016. Each sampling site (Folsom 1 and Folsom 2) was a storm drain outfall that drained a low-density residential catchment in the City of Folsom. Dry season runoff measured at the sampling sites represents neighborhood outdoor water waste, mainly from landscape irrigation. During the drought of 2012–2016, median runoff flows were significantly reduced after local landscape irrigation regulations were enacted. The daily runoff pattern was also highly influenced by regulation, with reductions of daily peak runoff flows on 4–5 days in a week after watering regulations were enacted. The number of peak flow events in the daily runoff pattern were reduced during this period. In addition, a significant reduction in mean runoff volume occurred. Based on these results, the watering regulations enacted by the City of Folsom had a positive effect on reducing urban runoff from residential neighborhoods during the dry season. As the results are from monitoring sites in a relatively small geographical area, further work should evaluate reductions in irrigation runoff from other California locations to determine if this is a localized phenomenon.

Microgeographic speciation in a complex of Anatolian bush crickets facilitated by fast evolution of reproductive isolation.

Identifying the drivers of microgeographic speciation (i.e., speciation over small, local geographic scales) is key to understand the origin of speciose groups. Here, we use genomic data to infer the demographic processes underlying diversification in Poecilimon luschani (Orthoptera: Tettigoniidae), a species complex belonging to the most diverse genus of bush crickets from the Mediterranean region (>170 taxa) that comprises three recognized subspecies with small allopatric distributions in the topographically complex Teke Peninsula, southwestern Anatolia. Phylogenomic reconstructions that include all other taxa within the species group confirmed that subspecies of P. luschani originated from a common ancestor during the Pleistocene, supporting recent (<1 Ma) diversification within a small geographical area (ca. 120 × 80 km). Genetic clustering analyses corroborated the distinctiveness of each subspecies and the cohesiveness of their respective populations, with abrupt genetic discontinuities coinciding with contemporary range boundaries. Indeed, our analyses uncovered the presence of two sympatric cryptic sister lineages that diverged <300 ka ago and do not admix despite being co-distributed. Collectively, these results support that all lineages within the complex represent independently evolving entities corresponding to full-fledged species. Statistical evaluation of alternative models of speciation strongly supports a scenario of divergence in isolation followed by a period of limited gene flow during the last glacial period, when all lineages experienced marked expansions according to demographic reconstructions. Our study exemplifies how localized allopatric divergence and fast evolution of reproductive isolation can promote microgeographic speciation and explain the high rates of endemism characterizing biodiversity hotspots.

Rhynchospora mesoatlantica (Cyperaceae), an imperiled new species of beaksedge from eastern U.S.A.

Rhynchospora mesoatlanticasp. nov. (Cyperaceae) is described, illustrated, and compared with morphologically similar species. Rhynchospora mesoatlantica is known only from southern Delaware, southeastern Maryland, and southern New Jersey, all within the Mid-Atlantic region of the U.S.A. It inhabits sunny, wet margins of natural, shallow, nutrient-poor, seasonal ponds of the Coastal Plain. Narrow leaf blades; fruits obpyriform in outline; faces of mature fruits possessing a central, pale, well-demarcated disk; and fruit tubercle margins denticulate for most of their lengths indicate R. mesoatlantica is most similar to R. filifolia and R. harperi. Rhynchospora mesoatlantica is unique in its fruit dimensions, scales intermediate in length between R. filifolia and R. harperi, and relatively long fruit stipe. The NatureServe rank of Critically Imperiled and the IUCN rank of Endangered appear warranted for R. mesoatlantica because only six populations are known to be extant, most quite small and isolated; all populations occur within a small geographic area; populations have declined; and serious threats confront the survival of the species.

International Humanitarian Award.

The International Humanitarian Award recognizes extraordinary humanitarian services and activism by psychologists, including professional and volunteer work conducted primarily in the field with underserved populations. Award recipients are psychologists who, by their extraordinary service at a difficult time, improve the lives and contribute to the well-being of people in a large or small geographic area anywhere in the world. The 2023 recipient of the APA International Humanitarian Award was selected by the Board of Directors as recommended by the 2022 Committee on International Relations in Psychology. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Breast is best? A small-scale study exploring knowledge, attitudes, and training amongst community pharmacists about breastfeeding

Abstract Introduction The United Kingdom (UK) has one of the lowest breastfeeding rates in Europe, with the last Infant Feeding Survey showing a reduction from 81% of mothers initiating breastfeeding to 0.5% still breastfeeding at 12 months.1 It provides multiple health benefits for mothers and infants, and promoting breastfeeding is an important public health strategy, impacting the future health of the population. Community pharmacists are accessible and trusted healthcare professionals, giving expert advice on medication use and public health. Previous research has found a lack of knowledge and training on medicines use during lactation and breastfeeding-related conditions, often leading to unnecessary cessation of breastfeeding.2 Aim To explore breastfeeding knowledge, attitudes, and training amongst community pharmacists. Methods Following ethical approval from Kingston University and a pilot study, a paper questionnaire comprising 24 mixed-style questions was distributed to community pharmacists in the London boroughs of Ealing and Brent. The Raosoft online calculator was used to calculate the sample size of 110 pharmacists at 95% confidence interval. Semi-structured interviews were conducted using a validated interview guide. Analyses were performed using SPSS software alongside thematic analysis for interview responses. Results Response rate was 75% (n=82/110) and 9 pharmacists participated in the interviews. 47.6% (n=39/82) pharmacists rated their current breastfeeding knowledge as neither poor nor good or lower. Only 11% (n=9/82) pharmacists stated the duration of breastfeeding should be greater than 2 years. 27.2% (n=12/44) pharmacists felt slightly or not confident providing over-the-counter medicines to breastfeeding patients compared to 61.4% (n=27/44) when making recommendations for breastfeeding-related conditions such as mastitis or nipple thrush. Thematic analysis highlighted the need for further research into medication use safety during lactation as pharmacists lacked confidence to make recommendations based on Summary of Product Characteristics (SPC). 50% (n=41/82) pharmacists stated their knowledge was obtained from personal experience. 100% (n=82/82) of participants agreed that more education and training must be provided, and thematic analysis showed pharmacists would prefer a spiralled approach starting at university level, through foundation year training and post-registration. Discussion/Conclusion The majority of pharmacists showed somewhat or high confidence advising patients about over-the-counter medication safety during lactation, but this was reversed when confronted with breastfeeding-related conditions, in contrast to findings from Byerley2 where the majority of pharmacists were not confident about medicines advice. Pharmacists have a positive attitude towards breastfeeding with all desiring further training. Changes to pharmacy education and training are needed to develop pharmacists’ understanding on breastfeeding generally and medicines safety with the need for robust evidence from clinical trials to improve pharmacist’s confidence to advise appropriately,3 or at least to educate pharmacists about specialised resources such as the Drug and Lactation Database or Breastfeeding Network. Study strengths included providing information about an area with limited literature and a reasonable response rate. Limitations included a small sample size in a small geographical area. Only community pharmacists were surveyed but with evolving roles, it would benefit surveying primary care pharmacists.

Genetic and technological diversity of Streptococcus thermophilus isolated from the Saint-Nectaire PDO cheese-producing area.

Streptococcus thermophilus is of major importance for cheese manufacturing to ensure rapid acidification; however, studies indicate that intensive use of commercial strains leads to the loss of typical characteristics of the products. To strengthen the link between the product and its geographical area and improve the sensory qualities of cheeses, cheese-producing protected designations of origin (PDO) are increasingly interested in the development of specific autochthonous starter cultures. The present study is therefore investigating the genetic and functional diversity of S. thermophilus strains isolated from a local cheese-producing PDO area. Putative S. thermophilus isolates were isolated and identified from milk collected in the Saint-Nectaire cheese-producing PDO area and from commercial starters. Whole genomes of isolates were sequenced, and a comparative analysis based on their pan-genome was carried out. Important functional properties were studied, including acidifying and proteolytic activities. Twenty-two isolates representative of the diversity of the geographical area and four commercial strains were selected for comparison. The resulting phylogenetic trees do not correspond to the geographical distribution of isolates. The clustering based on the pan-genome analysis indicates that isolates are divided into five distinct groups. A Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation of the accessory genes indicates that the accessory gene contents of isolates are involved in different functional categories. High variability in acidifying activities and less diversity in proteolytic activities were also observed. These results indicate that high genetic and functional variabilities of the species S. thermophilus may arise from a small (1,800 km2) geographical area and may be exploited to meet demand for use as autochthonous starters.

The effect of ambient air pollution on birth outcomes in Norway

BackgroundAmbient air pollution can be harmful to the fetus even in countries with relatively low levels of pollution. Most of the established literature estimates the association between air pollution and health rather than causality. In this paper, I examine the causal effects of ambient air pollution on birth outcomes in Norway.MethodsWith the large sample size and geographic division of sub-postal codes in Norway, I can control for a rich set of spatio-temporal fixed effects to overcome most of the endogeneity problems caused by the choice of residential area and date of delivery. After controlling for a rich set of spatio-temporal fixed effects, my paper uses the variance in ambient air pollutant concentrations over narrow time intervals and in a small geographic area of Norway to determine how prenatal air pollution exposure affects birth outcomes. My data contain extensive information about parents as well as meteorological conditions that can be used to control for potential confounding factors.ResultsI find that prenatal exposure to ambient nitric oxide in the last trimester causes significant birth weight and birth length loss under the same sub-postcode fixed effects and calendar month fixed effects, whereas other ambient air pollutants such as nitrogen dioxide and sulfur dioxide appear to be at safe levels for the fetus in Norway. In addition, the marginal adverse effect of ambient nitric oxide is larger for newborns with disadvantaged parents. Both average concentrations of nitric oxide and occasional high concentration events can adversely affect birth outcomes.ConclusionsPrenatal exposure to NO pollution has an adverse effect on birth outcomes. This suggests that government and researchers should pay more attention to examining NO pollution and that health care providers need to advise pregnant women about the risks of air pollution during pregnancy.

Prediction of Ross River Virus Incidence Using Mosquito Data in Three Cities of Queensland, Australia.

Ross River virus (RRV) is the most common mosquito-borne disease in Australia, with Queensland recording high incidence rates (with an annual average incidence rate of 0.05% over the last 20 years). Accurate prediction of RRV incidence is critical for disease management and control. Many factors, including mosquito abundance, climate, weather, geographical factors, and socio-economic indices, can influence the RRV transmission cycle and thus have potential utility as predictors of RRV incidence. We collected mosquito data from the city councils of Brisbane, Redlands, and Mackay in Queensland, together with other meteorological and geographical data. Predictors were selected to build negative binomial generalised linear models for prediction. The models demonstrated excellent performance in Brisbane and Redlands but were less satisfactory in Mackay. Mosquito abundance was selected in the Brisbane model and can improve the predictive performance. Sufficient sample sizes of continuous mosquito data and RRV cases were essential for accurate and effective prediction, highlighting the importance of routine vector surveillance for disease management and control. Our results are consistent with variation in transmission cycles across different cities, and our study demonstrates the usefulness of mosquito surveillance data for predicting RRV incidence within small geographical areas.

Swarm Intelligence Response Methods Based on Urban Crime Event Prediction

Cities attract a large number of inhabitants due to their more advanced industrial and commercial sectors and more abundant and convenient living conditions. According to statistics, more than half of the world’s population resides in urban areas, contributing to the prosperity of cities. However, it also brings more crime risks to the city. Crime prediction based on spatiotemporal data, along with the implementation of multiple unmanned drone patrols and responses, can effectively reduce a city’s crime rate. This paper utilizes machine learning and data mining techniques, predicts crime incidents in small geographic areas with short timeframes, and proposes a random forest algorithm based on oversampling, which outperforms other prediction algorithms in terms of performance. The research results indicate that the random forest algorithm based on oversampling can effectively predict crimes with an accuracy rate of up to 95%, and an AUC value close to 0.99. Based on the crime prediction results, this paper proposes a multi-drone patrol response strategy to patrol and respond to predicted high-crime areas, which is based on target clustering and combined genetic algorithms. This strategy may help with the pre-warning patrol planning within an hourly range. This paper aims to combine crime event predictions with crowd-sourced cruise responses to proactively identify potential crimes, providing an effective solution to reduce urban crime rates.

Water, Culture and International Institutions

This article focuses on the integration of different values of water, ranging from intrinsic to emotional, in international treaties and transboundary organizations. After introducing the “four worlds of water” (Wolf 2017), we discuss the increased recognition of locally based cultural and spiritual values of water in global conventions, international freshwater treaties and regional river basin organizations. Global conventions generally use more technical and broad formulations and international treaties tend to focus on small geographic areas and the need to protect water, and environmental resources associated with water, while the cultural impacts of water management decisions on local communities are most apparent at the governance level of regional organizations.

Mapping regional variability of exclusive breastfeeding and its determinants at different infant’s age in Tanzania

IntroductionDespite its numerous benefits, exclusive breastfeeding (EBF) remains an underutilized practice. Enhancing EBF uptake necessitates a focused approach targeting regions where its adoption is suboptimal. This study aimed to investigate regional disparities in EBF practices and identify determinants of EBF among infants aged 0–1, 2–3, and 4–5 months in Tanzania.MethodsThis cross-sectional study utilized data from the 2015/16 Tanzania Demographic and Health Survey. A total of 1,015 infants aged 0–5 met the inclusion criteria, comprising 378 aged 0–1 month, 334 at 2–3 months, and 303 at 4–5 months. EBF practices were assessed using a 24-hour recall method. A generalized linear mixed model, with fixed covariates encompassing infant and maternal attributes and clusters for enumeration areas (EAs) and regions, was employed to estimate EBF proportions.ResultsRegional disparities in EBF were evident among infants aged 0–1, 2–3, and 4–5 months, with decline in EBF proportions as an infant’s age increases. This pattern was observed nationwide. Regional and EA factors influenced the EBF practices at 0–1 and 2–3 months, accounting for 17–40% of the variability at the regional level and 40–63% at the EA level. Literacy level among mothers had a significant impact on EBF practices at 2–3 months (e.g., women who could read whole sentences; AOR = 3.2, 95% CI 1.1,8.8).ConclusionRegional disparities in EBF proportions exist in Tanzania, and further studies are needed to understand their underlying causes. Targeted interventions should prioritize regions with lower EBF proportions. This study highlights the clustering of EBF practices at 0–1 and 2–3 months on both regional and EA levels. Conducting studies in smaller geographical areas may enhance our understanding of the enablers and barriers to EBF and guide interventions to promote recommended EBF practices.

Review of a Comprehensive Analysis of Planning, Functionality, Control, and Protection for Direct Current Microgrids

Microgrids have emerged as a feasible solution for consumers, comprising Distributed Energy Resources (DERs) and local loads within a smaller geographical area. They are capable of operating either autonomously or in coordination with the main power grid. As compared to Alternating Current (AC) microgrid, Direct Current (DC) microgrid helps with grid modernisation, which enhances the integration of Distributed and Renewable energy sources, which promotes energy efficiency and reduces losses. The integration of energy storage systems (ESS) into microgrids has garnered significant attention due to the capability of ESS to store energy during periods of low demand and then provide it during periods of high demand. This research includes planning, operation, control, and protection of the DC microgrid. At the beginning of the chapter, a quick explanation of DC microgrids and their advantages over AC microgrids is provided, along with a thorough evaluation of the various concerns, control techniques, challenges, solutions, applications, and overall management prospects associated with this integration. Additionally, this study provides an analysis of future trends and real-time applications, which significantly contributes to the development of a cost-effective and durable energy storage system architecture with an extended lifespan for renewable microgrids. Therefore, providing a summary of the anticipated findings of this scholarly paper contributes to the advancement of a techno-economic and efficient integration of ESS with a prolonged lifespan for the use of green microgrids.

High TGM1 Allelic Heterogeneity causing Lamellar ichthyosis in a small geographic area in South Mexico: Another Example of the “Réunion Paradox”

Lamellar ichthyosis (LI) is an autosomal recessive congenital ichthyosis characterized by generalized dry skin and severe scaling. It is caused by biallelic mutations in the TGM1 gene, however molecular data from non-Caucasian populations are limited. Results of genetic-molecular analysis of a group of LI pedigrees originating from two close small populations from south Mexico are presented. LI affected individuals belonging to 9 apparently unrelated families were studied. Exome sequencing or Sanger sequencing in probands from each family was carried out. Furthermore, DNA from 294 unaffected subjects from one of the communities were Sanger sequenced to determine the carrier frequency of the c.427C > T TGM1 variant. Five different TGM1 pathogenic variants, either in homozygous or in compound heterozygous state, were demonstrated in affected subjects. The two most common variants were c.427C > T (p.Arg143Cys) and c.1159+1G > T. A novel c.1645+1G > T TGM1 pathogenic allele was recognized. Carrier frequency analysis identified a total of 23 individuals heterozygous for the c.427C > T variant, predicting a prevalence of 78 carriers per 1000 inhabitants in the community. A high TGM1 allelic heterogeneity with 5 different LI-causing alleles in a limited geographic area was demonstrated. While the occurrence of homozygosity for a founder mutation is expected in small populations with high frequency of a particular autosomal recessive disorder, the occurrence of multiple pathogenic alleles has been previously described, a situation known as the Reúnion paradox. Our results expand the current knowledge of the mutational spectrum of TGM1-linked LI.

High seasonal overlap in habitat suitability in a non-migratory High Arctic ungulate

Understanding drivers of space use and habitat selection is essential for management and conservation, especially under rapid environmental change. Here, we develop summer and winter habitat suitability models for the endemic wild Svalbard reindeer (Rangifer tarandus platyrhynchus). The High Arctic Svalbard tundra is currently subject to the fastest temperature increases on Earth, and reindeer spatial responses to associated environmental change are strongly restricted due to landscape barriers (including 60% glacial coverage) and lack of sea ice as movement corridors. We used an extensive dataset of GPS-collared adult females (2009–2018; N = 268 individual-years) to model seasonal habitat selection as a function of remotely sensed environmental variables , and subsequently built habitat suitability models using an ensemble modelling framework. As expected, we found that reindeer preferred productive habitats, described by the normalized difference vegetation index (NDVI) and plant biomass (derived from a vegetation map), in both seasons. This was further supported by selection for bird cliff areas, rich in forage, improving habitat suitability especially in winter. Contrary to our expectations, the terrain variables had similar, impact on habitat suitability in the two seasons, except for use of higher elevations in winter, likely related to improved forage access due to less snow. Suitable habitat patches covered only a small proportion of the landscape and were highly clustered in both seasons. About 13.0% of the total land area was suitable in both seasons, while summer-only and winter-only areas contributed a marginal addition of around 4.7% and 1.5%, respectively. This suggests, that unlike many continental and migratory Rangifer populations, even small geographic areas may encompass suffiscient suitable habitat. These first archipelago-wide habitat suitability models provide seasonal baseline maps relevant for the management and conservation of Svalbard reindeer, particularly under rapid environmental alterations from climate change.

Heterogeneous microgeographic genetic structure of the common cockle (Cerastoderma edule) in the Northeast Atlantic Ocean: biogeographic barriers and environmental factors.

Knowledge of genetic structure at the finest level is essential for the conservation of genetic resources. Despite no visible barriers limiting gene flow, significant genetic structure has been shown in marine species. The common cockle (Cerastoderma edule) is a bivalve of great commercial and ecological value inhabiting the Northeast Atlantic Ocean. Previous population genomics studies demonstrated significant structure both across the Northeast Atlantic, but also within small geographic areas, highlighting the need to investigate fine-scale structuring. Here, we analysed two geographic areas that could represent opposite models of structure for the species: (1) the SW British Isles region, highly fragmented due to biogeographic barriers, and (2) Galicia (NW Spain), a putative homogeneous region. A total of 9250 SNPs genotyped by 2b-RAD on 599 individuals from 22 natural beds were used for the analysis. The entire SNP dataset mostly confirmed previous observations related to genetic diversity and differentiation; however, neutral and divergent SNP outlier datasets enabled disentangling physical barriers from abiotic environmental factors structuring both regions. While Galicia showed a homogeneous structure, the SW British Isles region was split into four reliable genetic regions related to oceanographic features and abiotic factors, such as sea surface salinity and temperature. The information gathered supports specific management policies of cockle resources in SW British and Galician regions also considering their particular socio-economic characteristics; further, these new data will be added to those recently reported in the Northeast Atlantic to define sustainable management actions across the whole distribution range of the species.

Progress Toward Poliomyelitis Eradication - Pakistan, January 2022-June 2023.

Since the establishment of the Global Polio Eradication Initiative in 1988, Pakistan remains one of only two countries (along with Afghanistan) with continued endemic transmission of wild poliovirus (WPV). This report describes Pakistan's progress toward polio eradication during January 2022-June 2023. During 2022, Pakistan reported 20 WPV type 1 (WPV1) cases, all of which occurred within a small geographic area encompassing three districts in south Khyber Pakhtunkhwa. As of June 23, only a single WPV1 case from Bannu district in Khyber Pakhtunkhwa province has been reported in 2023, compared with 13 cases during the same period in 2022. In addition, 11 WPV1 isolates have been reported from various environmental surveillance (ES) sewage sampling sites to date in 2023, including in Karachi, the capital of the southern province of Sindh. Substantial gaps remain in the quality of supplementary immunization activities (SIAs), especially in poliovirus reservoir areas. Despite the attenuation and apparently limited geographic scope of poliovirus circulation in Pakistan, the isolation of WPV1 from an ES site in Karachi is cause for concern about the actual geographic limits of transmission. Interrupting WPV1 transmission will require meticulous tracking and sustained innovative efforts to vaccinate children who are regularly missed during SIAs and rapidly responding to any new WPV1 isolations.