Small Burst Research Articles

Effect of repair factors on multi-level optimization of maintenance planning for corroded pipelines considering different failure modes

The use of pipelines for transporting fluids is essential and has been growingsignificantly in the oil and gas industry. Although pipelines are one of the safestmethods to transport these materials, it is necessary to consider the risks of failures inthe design, as such incidents can cause considerable damage to the population, theenvironment, and the infrastructure. In this context, the aging of the pipeline networkhas been extensively studied, and corrosion is one of the main concerns regardingstructural integrity. Therefore, adequate maintenance planning is essential to ensure thesafety and cost-effectiveness of the sector, as effective pipeline integrity managementcan reduce expenses and maintain safety standards throughout the operational life of thepipeline. In this scenario, inspection and maintenance planning research has becomeincreasingly relevant; recent studies have utilized reliability analysis through MonteCarlo simulations to define optimal maintenance schedules for corroded pipelines,aiming to minimize operational costs associated with pipeline management. This paperinvestigates the influence of repair factors on the estimated total costs of inspectionschedules optimized for a given number of inspections, considering only one failuremode and the combination of these (small leak, burst, and rupture). For this, acombination of methodologies already validated and presented in previous works isused, in which a multilevel optimization is carried out by predefining an upper limit forthe maximum failure probability, which will act as a constraint on the optimizationproblem. The reliability analysis will be performed using Monte Carlo simulations,which are commonly used in the literature. Finally, preliminary results indicated that thefailure mode strongly influences the final cost, so the results considering only onefailure mode are expected to differ considerably.

A probabilistic framework for external pitting corrosion growth modelling for buried steel pipelines considering soil properties

This paper introduces a novel framework for developing reliable probabilistic predictive corrosion growth models for buried steel pipelines using pipeline inspection data. The framework adopts a power-law function of time model formulation, accounting for nonconstant damage growth rates, and considers the correlation between defect depth and length growth models. The proposed framework explicitly incorporates local influential soil properties in the model formulation; thus, it requires no segmentation and homogenous defect growth assumption and provides defect-specific growth models. The framework is applicable regardless of the availability of matched or non-matched defect data. For corrosion initiation time estimation, two different approaches are proposed: one is to use a Poisson process to account for defect occurrence, which can also predict newly generated defects since the last inspection, and the other is to use multivariate linear regression of soil and pipe properties. The statistics of unknown model parameters are assessed using a Bayesian updating framework in which the model error can be incorporated. The proposed framework is applied using two different sets of data: one set of inline inspection (ILI) data and one set of field excavation data. A case study is conducted, where time-dependent system reliability of an in-service pipeline is assessed considering small leak and burst failure modes using the developed defect growth models. The impact of the growth model accuracy on the probability of failure is investigated, and the importance analysis is performed to identify the most influential random variables to the probability of failure.

Time varying reliability analysis of corroded gas pipelines using copula and importance sampling

Ensuring the safety of gas pipelines is crucial for the reliable and efficient transportation of natural gas. This study introduces a methodology for evaluating the time-varying reliability of corroded gas pipelines. The proposed approach employs both importance sampling and copula theory to effectively address the uncertainties associated with random variables involved and provide efficient estimates of failure probability. Small leak and burst failure modes are specifically investigated within this study. The methodology first establishes a step-by-step procedure for analysis. Subsequently, the study investigates the failure probability of corroded gas pipelines, taking into account both small leak and burst failures, under scenarios involving single and multiple defects. Furthermore, the study examines the influence of correlation strength and dependence structure among the involved random variables using the copula theory. Additionally, the generation of newly formed defects is thoroughly investigated, along with its impact on failure probability. Numerical examples are provided to evaluate the performance of the proposed methodology, comparing it with the benchmark failure probability estimated through Monte Carlo simulation. The results validate the precision and efficiency of the proposed methodology, while offering practical and insightful suggestions for reliability analysis of real-world gas pipelines.

Analysis of the life cycle of Helicobacter pylori bacteriophage KHP40 belonging to the genus Schmidvirus.

Bacteriophage (phage) KHP40 was previously isolated from the supernatant of a culture of Helicobacter pylori KMT83 cells. In this study, we analysed the infection characteristics of KHP40, phage release pattern from KMT83 cells, and state of KHP40 DNA in KMT83 cells. The findings revealed that KHP40 phage showed varied adsorption efficiencies for different strains, long latent periods, and small burst sizes. Additionally, KHP40 activity was maintained at pH 2.5-12. KHP40 phages were released during the vegetative growth phase of the KMT83 cells. PCR analysis demonstrated that KHP40 DNA was stably maintained in KMT83 clones. Next-generation sequencing analysis revealed the presence of two distinct types of circular double-stranded DNA in H. pylori KMT83 cells. One was an H. pylori-specific DNA consisting of 1 578 403bp, and the other was a 26 412-bp sequence that represented the episomal form of phage KHP40 DNA. Furthermore, defective KHP40-lysogenic DNA was detected in the H. pylori-specific DNA, the deleted portion of which appeared to have been transferred to another location in the bacterial genome. These findings indicate that KHP40 DNA exists in both episomal and defectively lysogenized states in KMT83 cells, and active phages are produced from KHP40-episomal DNA.

Pipelines reliability assessment considering corrosion-related failure modes and probability distributions characteristic using subset simulation

A methodology using the subset simulation and the modified Metropolis-Hastings algorithm with delayed rejection (SS-MMHDR) is developed to evaluate the time-dependent reliability of corroded pipelines under multiple failure modes. This methodology considers the competition between the small leak and burst failures and the correlated non-normal distribution of the defect variables, and characterizes the defect geometric probability distribution from extensive inspection data. Results demonstrate that SS-MMHDR has high accuracy and low variability in evaluating the pipeline failures, outperforming the SS-MMH and Monte Carlo simulation algorithms, especially under low probability levels, but with slightly increased computation time. The Generalized Extreme Value Type II (GEVII) distribution is the optimal distribution for internal corrosion defect lengths from the Kolmogorov-Smirnov test. Lognormal, Weibull and Normal distributions provide more conservative estimates on burst and composite failure probabilities and limiting maintenance times than GEVII. Effects of operating pressure and its variability, corrosion rate on failure probability, and pipeline limiting maintenance time are also revealed through numerical cases.

Self-assembled mesoporous particles of Antheraea pernyi silk fibroin for encapsulation and sustained release of 5-fluorouracil

Mesoporous particles have attracted considerable attention in drug delivery system, because they can offer fast mass transport and overcome the diffusion restriction as occurred in micro/nano- porous particles. Conventional mesoporous particles are generally dominated by inorganic compounds, little concern has been raised over biodegradable organic polymers, especially silk fibroin. Here, we show that Antheraea pernyi (A. pernyi) silk fibroin, rich in Arg-Gly-Asp (RGD) moiety, can be developed into mesoporous particles via self-assembly in a direct and eco-friendly (without any organic solvents) manner for sustained release of 5-fluorouracil (5-FU). By cultivating a mild condition at 40 ℃, a mesoporous structure was successfully achieved without any foreign organic solvents. The self-assembled mesoporous particles were quasi-spherical in shape with an average diameter of 1.58 µm. Mesopores ran through the entire particles to construct a divergent structure with external pore diameters ranging from 30 to 60 nm and internal pore width of 6.225 nm. The mesoporous particles were predominated by β-sheet conformation (∼45%), which effectively reduced the weight-loss ratio to 2.6%. 5-FU was encapsulated into the mesoporous particles by hydrogen bonding and van der Waals force as well as ionic interactions. Drug loading efficiency and encapsulation efficiency were found to be 26.24% and 50.36%, respectively. The mesoporous particles enabled sustained drug release, with approximately 90% release after 110 h. After a small burst release (20%) of the surface-bound 5-FU, a slower linear release dominated by Fickian-diffusion occurred. These features together with the excellent biocompatibility of A. pernyi silk indicated that the self-assembled mesoporous particles would be an ideal carrier for sustained drug release to meet critical requirements of biomaterials. Furthermore, without any foreign materials, this one-step strategy may offer new green chemistry approaches as well as options to load bioactive drugs.

Characterization of a novel genus of jumbo phages and their application in wastewater treatment

Phages widely exist in numerous environments from wastewater to deep ocean, representing a huge virus diversity, yet remain poorly characterized. Among them, jumbo phages are of particular interests due to their large genome (>200kb) and unusual biology. To date, only six strains of jumbo phages infecting Klebsiella pneumoniae have been described. Here, we report the isolation and characterization of two jumbo phages from hospital wastewater representing the sixth genus: φKp5130 and φKp9438. Both phages showed lytic activity against broad range of clinical antibiotic-resistant K. pneumoniae strains and distinct physiology including long latent period, small burst size, and high resistance to thermal and pH stress. The treatment of sewage water with the phages cocktail resulted in dramatic decline in K. pneumoniae population. Overall, this study provides detailed molecular and genomics characterization of two novel jumbo phages, expands viral diversity, and provides novel candidate phages to facilitate environmental wastewater treatment.

In situ Hydrogels Prepared by Photo-initiated Crosslinking of Acrylated Polymers for Local Delivery of Antitumor Drugs

A triblock copolymer was synthesized by ring opening polymerization of ε‐caprolactone in the presence of poly(ethylene glycol) (PEG). The resulted PCL-PEG-PCL triblock copolymer, PEG and monomethoxy (MPEG) were functionalized by end group acrylation. NMR and FT-IR analyses evidenced the successful synthesis and functionalization of polymers. A series of photo-crosslinked hydrogels composed of acrylated PEG-PCL-Acr and MPEG-Acr or PEG-Acr were prepared by exposure to visible light using lithium phenyl-2,4,6-trimethylbenzoylphosphinate as initiator. The hydrogels present a porous and interconnected structure as shown by SEM. The swelling performance of hydrogels is closely related to the crosslinking density and hydrophilic content. Addition of MPEG or PEG results in increase in water absorption capacity of hydrogels. In vitro degradation of hydrogels was realized in the presence of a lipase from porcine pancreas. Various degradation rates were obtained which mainly depend on the hydrogel composition. MTT assay confirmed the good biocompatibility of hydrogels. Importantly, in situ gelation was achieved by irradiation of a precursor solution injected in the abdomen of mice. Doxorubicin (DOX) was selected as a model antitumor drug to evaluate the potential of hydrogels in cancer therapy. Drug-loaded hydrogels were prepared by in situ encapsulation. In vitro drug release studies showed a sustained release during 28 days with small burst release. DOX-loaded hydrogels exhibit antitumor activity against A529 lung cancer cells comparable to free drug, suggesting that injectable in situ hydrogel with tunable properties could be most promising for local drug delivery in cancer therapy.

Isolation and characterization of a novel phage belonging to a new genus against Vibrio parahaemolyticus

BackgroundVibrio parahaemolyticus is a major foodborne pathogen that contaminates aquatic products and causes great economic losses to aquaculture. Because of the emergence of multidrug-resistant V. parahaemolyticus strains, bacteriophages are considered promising agents for their biocontrol as an alternative or supplement to antibiotics. In this study, a lytic vibriophage, vB_VpaM_R16F (R16F), infecting V. parahaemolyticus 1.1997T was isolated, characterized and evaluated for its biocontrol potential.MethodsA vibriophage R16F was isolated from sewage from a seafood market with the double-layer agar method. R16F was studied by transmission electron microscopy, host range, sensitivity of phage particles to chloroform, one-step growth curve and lytic activity. The phage genome was sequenced and in-depth characterized, including phylogenetic and taxonomic analysis.ResultsR16F belongs to the myovirus morphotype and infects V. parahaemolyticus, but not nine other Vibrio spp. As characterized by determining its host range, one-step growth curve, and lytic activity, phage R16F was found to highly effective in lysing host cells with a short latent period (< 10 min) and a small burst size (13 plaque-forming units). R16F has a linear double-stranded DNA with genome size 139,011 bp and a G + C content of 35.21%. Phylogenetic and intergenomic nucleotide sequence similarity analysis revealed that R16F is distinct from currently known vibriophages and belongs to a novel genus. Several genes (e.g., encoding ultraviolet damage endonuclease and endolysin) that may enhance environmental competitiveness were found in the genome of R16F, while no antibiotic resistance- or virulence factor-related gene was detected.ConclusionsIn consideration of its biological and genetic properties, this newly discovered phage R16F belongs to a novel genus and may be a potential alternate biocontrol agent.

Two modes of midfrontal theta suggest a role in conflict and error processing

Midfrontal theta increases during scenarios when conflicts are successfully resolved. Often considered a generic signal of cognitive control, its temporal nature has hardly been investigated. Using advanced spatiotemporal techniques, we uncover that midfrontal theta occurs as a transient oscillation or “event” at single trials with their timing reflecting computationally distinct modes. Single-trial analyses of electrophysiological data from participants performing the Flanker (N = 24) and Simon task (N = 15) were used to probe the relationship between theta and metrics of stimulus-response conflict. We specifically investigated “partial errors”, in which a small burst of muscle activity in the incorrect response effector occurred, quickly followed by a correction. We found that transient theta events in single trials could be categorized into two distinct theta modes based on their relative timing to different task events. Theta events from the first mode occurred briefly after the task stimulus and might reflect conflict-related processing of the stimulus. In contrast, theta events from the second mode were more likely to occur around the time partial errors were committed, suggesting they were elicited by a potential upcoming error. Importantly, in trials in which a full error was committed, this “error-related theta” occurred too late with respect to the onset of the erroneous muscle response, supporting the role of theta also in error correction. We conclude that different modes of transient midfrontal theta can be adopted in single trials not only to process stimulus-response conflict, but also to correct erroneous responses.

Drug release mechanisms of high-drug-load, melt-extruded dexamethasone intravitreal implants

Ozurdex is an FDA–approved sustained-release, biodegradable implant formulated to deliver the corticosteroid dexamethasone to the posterior segment of the eye for up to 6 months. Hot-melt extrusion is used to prepare the 0.46 mm × 6 mm, rod-shaped implant by embedding the drug in a matrix of poly(lactic-co-glycolic acid) (PLGA) in a 60:40 drug:polymer ratio by weight. In our previous work, the Ozurdex implant was carefully studied and reverse engineered to produce a compositionally and structurally equivalent implant for further analysis. In this work, the reverse-engineered implant was thoroughly characterized throughout the in vitro dissolution process to elucidate the mechanisms of controlled drug release. The implant exhibited a triphasic release profile in 37 °C normal saline with a small burst release (1–2 %), a one-week lag phase with limited release (less than 10 %), and a final phase where the remainder of the dose was released over 3–4 weeks. The limited intermolecular interaction between dexamethasone and PLGA rendered the breakdown of the polymer the dominating mechanism of controlled release. A close relationship between drug release and total implant mass loss was observed. Unique chemical and structural differences were seen between the core of the implant and the implant surface driven by diffusional limitations, autocatalytic hydrolysis, and osmotic effects.

Pectin based biologic Velcro effectively seals traumatic solid organ and small bowel injuries.

Injuries to the liver and small bowel are common in multiple injuries. While there are currently a variety of accepted damage-control techniques to expeditiously manage such injuries, morbidity and mortality remain high. Pectin polymers have previously been shown to effectively seal visceral organ injuries ex vivo through physiochemical entanglement with the glycocalyx. We sought to compare the standard of care for the management of penetrating liver and small bowel injuries with a pectin-based bioadhesive patch in a live animal model. Fifteen adult male swine underwent a laparotomy with standardized laceration to the liver. Animals were randomized to one of three treatment arms: packing with laparotomy pads (n = 5), suture repair (n = 5), or pectin patch repair (n = 5). Following 2 hours of observation, fluid was evacuated from the abdominal cavity and weighed. Next, a full-thickness small bowel injury was created, and animals were randomized to either a sutured repair (n = 7) or pectin patch repair (n = 8). The segment of bowel was then pressurized with saline, and the burst pressure was recorded. All animals survived the protocol to completion. There were no clinically significant differences between groups regarding baseline vitals or laboratory studies. On one-way analysis of variance, there was a statistically significant difference between groups regarding blood loss after liver repair (26 mL suture vs. 33 mL pectin vs. 142 mL packing, p < 0.01). On post hoc analysis, there was no statistically significant difference between suture and pectin ( p = 0.9). After repair, small bowel burst pressures were similar between pectin and suture repair (234 vs. 224 mm Hg, p = 0.7). Pectin-based bioadhesive patches performed similarly to the standard of care for the management of liver lacerations and full-thickness bowel injuries. Further testing is warranted to assess the biodurability of a pectin patch repair, as it may offer a simple option to effectively temporize traumatic intra-abdominal injuries.

When Plaquing Is Not Possible: Computational Methods for Detecting Induced Phages.

High-throughput sequencing of microbial communities has uncovered a large, diverse population of phages. Frequently, phages found are integrated into their bacterial host genome. Distinguishing between phages in their integrated (lysogenic) and unintegrated (lytic) stage can provide insight into how phages shape bacterial communities. Here we present the Prophage Induction Estimator (PIE) to identify induced phages in genomic and metagenomic sequences. PIE takes raw sequencing reads and phage sequence predictions, performs read quality control, read assembly, and calculation of phage and non-phage sequence abundance and completeness. The distribution of abundances for non-phage sequences is used to predict induced phages with statistical confidence. In silico tests were conducted to benchmark this tool finding that PIE can detect induction events as well as phages with a relatively small burst size (10×). We then examined isolate genome sequencing data as well as a mock community and urinary metagenome data sets and found instances of induced phages in all three data sets. The flexibility of this software enables users to easily include phage predictions from their preferred tool of choice or phage sequences of interest. Thus, genomic and metagenomic sequencing now not only provides a means for discovering and identifying phage sequences but also the detection of induced prophages.

The extent of intergalactic metal enrichment from galactic winds during the Cosmic Dawn

ABSTRACT One of the key processes driving galaxy evolution during the Cosmic Dawn is supernova feedback. This likely helps regulate star formation inside of galaxies, but it can also drive winds that influence the large-scale intergalactic medium. Here, we present a simple semi-analytic model of supernova-driven galactic winds and explore the contributions of different phases of galaxy evolution to cosmic metal enrichment in the high-redshift (z ≳ 6) Universe. We show that models calibrated to the observed galaxy luminosity function at z ∼ 6–8 have filling factors $\sim 1{{\%}}$ at z ∼ 6 and $\sim 0.1{{\%}}$ at z ∼ 12, with different star formation prescriptions providing about an order of magnitude uncertainty. Despite the small fraction of space filled by winds, these scenarios predict an upper limit to the abundance of metal-line absorbers in quasar spectra at $z \gtrsim 5$ which is comfortably above that currently observed. We also consider enrichment through winds driven by Pop III star formation in minihalos. We find that these can dominate the total filling factor at $z \gtrsim 10$ and even compete with winds from normal galaxies at z ∼ 6, at least in terms of the total enriched volume. But these regions have much lower overall metallicities, because each one is generated by a small burst of star formation. Finally, we show that Compton cooling of these supernova-driven winds at $z \gtrsim 6$ has only a small effect on the cosmic microwave background.

A novel methodology for reliability assessment of corroded natural gas pipelines considering two-level correlation

One of the main causes for the failure of natural gas pipeline is corrosion, which can induce severe consequences due to the inherent combustibility of natural gas. Therefore, it is of great significance to evaluate precisely the reliability of corroded natural gas pipelines. With regard to the confusion and apparent insufficient consideration of correlations surrounding current reliability assessment methods for corroded natural gas pipelines, this paper divides these correlations into two levels: “random variables correlation” (Level 1) and “spatial correlation” (Level 2), and then proposes a novel methodology for pipeline reliability assessment by examining the effects of a separate “two-level” correlation. Depending on the data provide by in-line inspection, the new methodology can calculate the probabilities of small leak and burst failure of a corroded pipeline with multiple interacting defects more accurately and more realistically. This paper uses Gaussian Copula approach to characterize the dependence among the random variables. The spatial correlation among defects is characterized by using an efficient probabilistic method. Finally, the influence of different kinds of correlation on reliability is studied by sensitivity analysis. The calculation results have indicated that the two-level correlation has great impact on the failure probability. The method in consideration of the two-level correlation is more objective and truly reflects the dynamic reliability changes of the corroded nature gas pipeline.

Long-acting injectable in situ gel of rasagiline: a patented product development.

Rasagiline has a certain potential in neuroprotection and delaying the progression of Parkinson's disease (PD). However, the poor pharmacokinetics (PK) characteristics of conventional oral tablets and poor medication compliance limit the optimal efficacy of rasagiline. Based on this, we designed and optimized a sustained-release rasagiline in situ gel based on in vitro release and in vivo PK results. Among them, we found for the first time that aluminum hydroxide can effectively shorten the lag phase and promote early and late release, making the daily release more uniform. After subcutaneous administration of the optimized gel formulation at a monthly dose, the Cmax (64ng/ml) was lower than that of free rasagiline (494ng/ml) administered subcutaneously at a daily dose and comparable to that of oral administration of Azilect® (59.1ng/ml) at a daily dose. In the meantime, the plasma concentration of rasagiline was mainly maintained at 5-10ng/ml for about 1month, and the active metabolite 1-aminoindane in plasma was also able to maintain a steady state. The rasagiline in situ gel has suitable viscosity and injectability, good repeatability of subcutaneous injection, and controllable impurities and can achieve sustained release in vivo with small burst release, which may have the clinical application advantages of maximizing the disease-modifying effect of rasagiline and improving medication compliance. The rasagiline in situ gel was optimized through the feedback of in vitro release and in vivo pharmacokinetics (PK), in which the addition of aluminum hydroxide had a modulating effect on uniform release. The gel has low burst release and maintains steady-state blood drug concentration for about 1 month.

Characteristics of a novel temperate bacteriophage against Staphylococcus arlettae (vB_SarS_BM31).

Staphylococcus arlettae is a rarely reported coagulase-negative staphylococcus (CoNS) isolated from infected humans and livestock. Observing phage-bacteria interaction could improve the understanding of bacterial pathogenetic mechanisms, providing foundational evidence for phage therapy or phage detection. Herein, we aimed to characterise and annotate a novel bacteriophage, vB_SarS_BM31 (BM31), specific to S. arlettae. This bacteriophage was isolated from a milk sample associated with bovine mastitis and collected in the Sichuan Province, China. The BM31 genome comprised a linear double-stranded DNA of 42,271 base pair in length with a G + C content of 34.59%. A total of 65 open reading frames (ORFs) were assembled from phage DNA, of which 29 were functionally annotated. These functional genes were divided into four modules: the structural, DNA packing and replication, lysis, and lysogeny modules. Holin (ORF25), lysin (ORF26), and integrase (ORF28) were located closely in the entire BM31 genome and were important for lyse or lysogeny cycle of BM31. The phage was identified as a temperate phage according to whole genome analysis and life cycle assay, with basic biological characteristics such as small burst size, short latency period, and narrow host range, consistent with the characteristics of the family Siphoviridae, subcluster B14 of the Staphylococcus bacteriophage. The present isolation and characterisation of BM31 contributes to the Staphylococcus bacteriophage database and provides a theoretical foundation for its potential applications. To the best of our knowledge, BM31 is the only shared and completely reported phage against S. arlettae in the entire public database.

Does game-irrelevant chatting stimulate high-value gifting in live streaming? A session-level perspective

Game live streaming has become increasingly popular and web streamer is widely recognized as a real career. A streamer is anticipated to entertain viewers, build relationships with them and solicit high-value gifts from viewers for profit. We explore game live streaming from a business aspect and find session-level factors of gifting with a data-driven approach. Previous studies extensively investigated the financial aspects of game live streaming and the social motivations behind this newly-emerged social media, but mostly from the individual level or as case studies. This study uses real live stream chatting and gifting data of over 1000 h and proposes metrics to proxy in-session behaviors for quantitative analysis of mass scale. We propose to use viewer chatting as a session-level measurement to better understand the motivations of high-value gifting. We find that the ratio of game-irrelevant chats (GIC) positively promotes high-value gifting, while small burst in game-irrelevant chats (spike) and frequent exchange of dominant topics (swap) negatively influence high-value gifting. Two sources of interactions, streamer and other viewers, elicit a framework to explain how in-session performance affects the high-value gifting. Specifically, parasocial relationship (PSR) with the streamer, and group identification (GI) with peer viewers are identified as mediators of the aforementioned three variables, and the two also positively influence high-value gifting. This research contributes to existing theories of gifting in live streaming context, and provides practical insights for live streaming platforms and streamers.

A Novel Alteromonas Phage Lineage with a Broad Host Range and Small Burst Size.

ABSTRACTAlteromonas is an opportunistic marine bacterium that persists in the global ocean and has important ecological significance. However, current knowledge about the diversity and ecology of alterophages (phages that infect Alteromonas) is lacking. Here, three similar phages infecting Alteromonas macleodii ATCC 27126T were isolated and physiologically characterized. Transmission electron microscopy revealed Siphoviridae morphology, with an oblate icosahedral head and a long noncontractile tail. Notably, these members displayed a small burst size (15–19 plaque-forming units/cell) yet an extensively broad host spectrum when tested on 175 Alteromonas strains. Such unique infection kinetics are potentially associated with discrepancies in codon usage bias from the host tRNA inventory. Phylogenetic analysis indicated that the three phages are closely evolutionarily related; they clustered at the species level and represent a novel genus. Three auxiliary metabolic genes with roles in nucleotide metabolism and putative biofilm dispersal were found in these phage genomes, which revealed important biogeochemical significance of these alterophages in marine ecosystems. Our isolation and characterization of these novel phages expand the current understanding of alterophage diversity, evolution, and phage–host interactions.IMPORTANCE The marine bacterium Alteromonas is prevalent in the global ocean with crucial ecological significance; however, little is known about the diversity and evolution of its bacteriophages that profoundly affect the bacterial communities. Our study characterized a novel genus of three newly isolated Alteromonas phages that exhibited a distinct infection strategy of broad host spectrum and small burst size. This strategy is likely a consequence of the viral trade-off between virulence and lysis profiles during phage–host coevolution, and our work provides new insight into viral evolution and infection strategies.

Novel Parenteral Nanoparticular Formulation of Anastrozole

The aim of the present project work is to prepare PEGylated Anastrozole-BSA nanoparticles were prepared by desolvation technique. Prepared nanoparticles were characterized in terms of particle size, scanning electron microscope (SEM), fourier transform infraredspectroscopy (FTIR) and differential scanningcalorimetry (DSC). In-vitrorelease studies were performed in phosphate buffersaline pH 7.4 at 37˚±0.5˚C for 1month. The mean particle size of obtained nanoparticles was 150-400 nm and was apparently spherical in shape, with smooth surface. DSC is done for the stability test for pure drug and sample. The thermogram of drug has not shifted for in the formulation compare to pure drug thermogram hence, the stability of formulation is not changed . FT-IR studies demonstrated that the drug was not changed in the formulation during the fabrication process. The encapsulation efficiency was about 48%. The Anastrozole-BSA nanoparticles exhibit a most interesting release profile with small initial burst followed by slower and controlled release.