Small Bean Research Articles

Anti-nutrient factors, nutritional components, and antioxidant activities of faba beans (Vicia faba L.) as affected by genotype, seed traits, and their interactions

This study explored how genotype, seed color, and seed weight affect major biochemical components in 95 faba bean accessions. Genotype variation significantly affected convicine, total tannin (TTC), total saponin, and total phenol (TPC) contents. Seed color and weight variations affected several parameters, with their interaction significantly affecting convicine, total vicine-convicine content (TVC), TTC, total polyunsaturated fatty acid (PUFA), and antioxidant activities. Genotype interaction with seed weight and seed color also significantly affected convicine, TVC, TPC, oleic acid, linoleic acid, PUFA, and ferric-reducing antioxidant power. Vicine, dietary fiber, total fat, crude protein, palmitic acid, and stearic acid contents remain unaffected by these factors. Multivariate analysis showed that brown and small beans had distinctive characteristics. Overall, this study demonstrated the connection between biochemical components, genotype, and seed traits in faba beans. Therefore, these factors should be considered when choosing faba bean genotypes for use in the food industry and breeding programs.

Predation efficacy of Chrysoperla carnea on two economically important stink bugs

Larvae of the green lacewing Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) are generalist predators feeding on a range of pests in agroecosystems. Little is known about the suitability of stink bugs as prey for lacewing larvae. In the present laboratory study, the predatory efficacy of C. carnea on two economically important stink bugs, Halyomorpha halys (Stål) and Nezara viridula (L.) (Hemiptera: Pentatomidae), was evaluated at three constant temperatures (16, 21, and 26 °C) in different arenas (10-cm diameter dishes with bean pod slices or caged small bean plants). We found that second and third instars of C. carnea readily prey on the first instars of both stink bug species. However, only third instars of C. carnea could successfully kill and feed on second instars of either pentatomid. The complexity of the arena, as well as the life stage of the prey and predator influenced the predation efficacy of C. carnea on stink bug nymphs, whereas temperature did not. The observed predation rates on the early instars of both stink bugs suggest that augmentative releases of C. carnea larvae may have some potential in greenhouse crops, particularly against N. viridula infestations, whereas in open field crops their natural populations may also contribute to pest suppression within the broader predator guild.

Detection of Heavy Metals in Some Edible Grains Obtained from Two Markets in Bauchi Metropolis, Nigeria

This study investigated the level of heavy metals (Cd, Cr, Cu, Fe, Pb and Zn) in grains sold in Central and Muda Lawal markets in Bauchi. Our objectives include assessing heavy metal concentrations in the grains and evaluating associated health risks. We utilized Atomic Absorption Spectrophotometry following acid digestion of grains to determine heavy metal concentrations. In Central market-sourced crops, the mean concentrations of Cd, Cr, Cu, Fe, Pb, and Zn were 0.06, 0.49, 0.21, 1.16, 0.15, and 0.17mg/kg respectively. Meanwhile, crops from Muda Lawal market exhibited mean concentrations of 0.06, 0.58, 0.17, 1.62, 0.1, and 0.22 mg/kg for Cd, Cr, Cu, Fe, Pb, and Zn, respectively. Cd, Cr, Cu, Pb, and Zn levels were below the permissible limits. However, Fe exceeded permissible limits in small beans, Syria maize, and white guinea corn from Central market, as well as in Syria maize, Pearl millet II, and white guinea corn from Muda Lawal market. The daily intake of Cd, Cu, and Pb from most studied crops exceeded the oral reference dose, posing potential health risks. Conversely, Cr, Fe, and Zn intake levels were within acceptable limits, posing minimal health risks. Hazard quotient and hazard index values were below 1, suggesting that crop consumption is unlikely to pose health risks.

Enhancement of Visible Light Antibacterial Activities of Cellulose Fibers from Lotus Petiole Decorated ZnO Nanoparticles.

Cellulose/ZnO (CZ) nanocomposites are promising antimicrobial materials known for their antibiotic-free nature, biocompatibility, and environmental friendliness. In this study, cellulose fibers extracted from lotus petioles were utilized as a substrate and decorated with various shapes of ZnO nanoparticles (NPs), including small bean, hexagonal ingot-like, long cylindrical, and hexagonal cylinder-shaped NPs. Increasing zinc salt molar concentration resulted in highly crystalline ZnO NPs forming and enhanced interactions between ZnO NPs and -OH groups of cellulose. The thermal stability and UV-visible absorption properties of the CZ samples were influenced by ZnO concentration. Notably, at a ZnO molar ratio of 0.1, the CZ 0.1 sample demonstrated the lowest weight loss, while the optical band gap gradually decreased from 3.0 to 2.45eV from the CZ 0.01 to CZ 1.0 samples. The CZ nanocomposites exhibited remarkable antibacterial activity against both Staphylococcus aureus (S. aureus, Gram-positive) and Escherichia coli (E. coli, Gram-negative) bacteria under visible light conditions, with a minimum inhibitory concentration (MIC) of 0.005mg/mL for both bacterial strains. The bactericidal effects increased with higher concentrations of ZnO NPs, even achieving 100% inhibition. Incorporating ZnO NPs onto cellulose fibers derived from lotus plants presents a promising avenue for developing environmentally friendly materials with broad applications in antibacterial and environmental fields.

Morphological description and histological structure of the Hedgehog Kidney (Hemiechinus auritus)

This study was aimed to recognize the morphological description and histological structure of the kidney in hedgehog (Hemiechinus auritus). The morphological results showed that the kidney has a small bean shape and reddish brown color. It was situated on both side of the anterior lumbar vertebra in the abdominal cavity behind the peritoneum. The kidney was surrounded by connective tissues capsule. Histological results clarify that the kidney was characterized by two an regions, outer called cortex and inner called medulla. Glomeruli densely distributed in the cortex region with mean diameter of 78 µm, also the cortex contains segments of proximal convoluted tubules and distal convoluted tubules. On the other hand the medulla region consist of both thick and thin segments of Henle’s loop in addition to sections of collecting tubules which forms radial structures which are known as the medullary rays. The histological results also showed that, the renal corpuscle is formed by the glomeruli that is surrounded by Bowman’s capsule, the proximal convoluted tubules, Henle’s loop, the distal convoluted tubules and collecting tubules. The proximal convoluted tubules connected with Bowman’s capsule and lined by simple cuboidal epithelial tissue based on a basement membrane while the free surface was covered with brush border. The results demonstrated that thin segments of the Henley’s loop were started from the end of the proximal convoluted tubule, extend inside of the medulla and lined by simple squamous epithelial tissue. Whilst the thick segments of the Henle’s loop were lined by simple cuboidal epithelial tissue. The current study clarify that, the distal convoluted tubules were lined by simple cuboidal epithelium rested on basement membrane and the free surface covered by small protrusions. Furthermore, the histological examination revealed that the collecting tubules were lined by simple cuboidal epithelium and the free surface of its cells had a cover of a few and short protrusions.

Como surgiu o feijão de terceira safra ou feijão de inverno no Brasil? Um pouco de história

The 3rd planting season, or irrigated or winter bean, appeared in the early 1980s. Small bean production at that time already existed, but took off with the emergence of large irrigation systems called central pivot, capable of irrigating areas of 120 ha with a 360 degree turn in just 24 h. Due to the milder climate planting season, irrigation and better management, the yields obtained exceed 2000 kg ha-1, substantially higher than the yields of other planting seasons. This article also presents some factors that result in high yields for the crop.

Effect of growing conditions and postharvest processing on arabica coffee bean physical quality features and defects

The individual and interaction effects of elevation, production system (PS), shade and postharvest processing (PHP) on the ratio of dry beans to red cherries and the green bean physical quality features and defects of arabica coffee in southwestern Ethiopia were evaluated. The results showed that, with increasing elevation, the proportions of the total defected beans and large beans decreased while that of medium beans increased. Moreover, the proportion of secondary defects, 1000 seed weight and bean volume were higher for lowland and midland coffees than for highland coffee, but bean density was higher for highland than for lowland and midland coffees. The proportion of the total defected beans was also higher for modern plantation coffee in lowland than for modern plantation and semi-plantation coffees in midland and highland, but the 1000 seed weight was lower for semi-plantation coffee in highland than for modern plantation coffee in lowland and midland. The ratio of primary and secondary defects respectively was higher for dry- and wet-processed coffee in lowland than for dry- and wet-processed coffees in midland and highland. But, the ratio of small beans was lower for wet-processed coffee in lowland than for dry-processed coffee across elevations. The ratio of dry beans to red cherries and the 100 beans volume were higher for wet-processed modern plantation and semi-plantation coffees in midland than for dry-processed coffees of both production systems across elevations. However, the ratio of large beans was higher (1) for wet-processed modern plantation coffee in lowland than for dry- and wet-processed coffees of both production systems across elevations, and (2) for coffee that was grown without shade and wet-processed in lowland than for other coffees. Bean density was higher for dry-processed modern plantation and semi-plantation coffee in midland and highland, respectively than for other coffees across elevations. Overall, these results underlined the primary effects of elevation and PS, and the complex interaction effects between PHP and PS or shade on the ratio of dry beans to red cherries and the physical features and defects of green arabica coffee beans.

Improving productivity and competitiveness of Kepahiang robusta coffee through innovation and partnership

Robusta coffee is a very strategic commodity in Kepahiang district, bengkulu province. This paper aims to (i) describe the overview, coffee quality and flavor, the financial feasibility of Kepahiang coffee farming, (ii) formulate technological innovations in increasing productivity, and (iii) formulate efforts to increase competitiveness. The paper was synthesized from literatures related robusta coffee, and it was conducted in 2019. All data were gathered from secondary data using documentation methods and the data from relevant literature were analyzed descriptively. Based on the synthesis, the productivity of Kepahiang robusta coffee is only around 774 kg/ha or still below its production potential. Kepahiang coffee was characterized as small beans with grade 5 and 6 while the total of flavor total was 80.50 - 85.50. Efforts to increase Kepahiang coffee productivity can be done through technological innovations such as the use of high yielding planting material, pest and disease control, pruning technology as well as postharvest and processing improvement. Increasing competitiveness can be done by developing partnerships that are designed through 4 (four) stages, namely phase 0, phase 1, phase 2 and phase 3. Technological innovation and partnership relationships are expected to increase productivity and competitiveness of Kepahiang robusta coffee.

Polyphenolic composition and antiproliferative activity of small malt bean extract (Glycyrrhiza glabra L.)

Polyphenolic composition and antiproliferative activity of small malt bean extract (Glycyrrhiza glabra L.)

High Temperature Rotational Rheology of the Seed Flour to Predict the Texture of Canned Red Kidney Beans (Phaseolus vulgaris).

The pasting profile of starchy tissues is conventionally measured by recording the apparent viscosity (η) in heating/cooling cycles. However, conventional rheometers show critical limitations when the starch is embedded in compact protein-rich cotyledon matrices, as occurs in pulses. In this work, the pasting profile of 13 red kidney beans (Phaseolus vulgaris) from the same cultivar but different growing locations was investigated using a heating/cooling cycle at higher temperature (130 °C) and pressurized conditions, using both water and brine as cooking solvents. It was hypothesized that the continuous measure of η at these conditions of flours from the dry seed would correlate with the texture, as determined by the mini-Kramer cell, of the beans after the entire process of soaking and canning. Furthermore, mechanistic answers were obtained by investigating their composition (starch, protein, and ash content) and physical properties (water holding capacity, seed ratio and weight). Interestingly, as opposed to the pasting profile at 95 °C, pasting indicators at 130 °C, including trough and final viscosity, strongly correlated with starch and protein content, seed coat ratio and, remarkably, with the firmness of the beans after canning when brine was incorporated. These results clearly show that small beans with a high protein content would bring about a more compact matrix that restricts starch from swelling and results in canned beans with a hard texture, which can be predicted by a lower pasting profile of the whole bean flour.

Small black bean (Rhynchosia volubilis) extract ameliorates gut microbial and metabolic perturbation in ovariectomized mice

Abstract Phytoestrogen is considered a promising natural remedy for various postmenopausal symptoms. However, most studies have focused on common soybean, Glycine max. Here, for the first time, we evaluated the in vivo effect of a distinct species, small black bean (Rhynchosia volubilis), on ovariectomized mice and investigated its impact on the intestinal microbiota and metabolic status. Ovariectomized mice exhibited significant body weight gain, a typical postmenopausal symptom, and microbial changes, such as decreased α-diversity; changes in microbial composition, especially abundances of the families Desulfovibrionaceae and Mogibacteriaceae and genus Dorea; and decreased amino acid and short-chain fatty acid levels. Administration of small black bean extract restores body weight and gut microbial perturbation to levels similar to those observed under normal conditions. Our data suggest that small black bean extract is a potential candidate functional food for treating postmenopausal symptoms via remodeling of the intestinal microbiome.

Cup quality attributes of Catimors as affected by size and shape of coffee bean (Coffea arabica L.)

ABSTRACTDuring the dry processing of coffee, beans are classified by size, shape, weight, and color, resulting in the commercial preparation requested by the client, who can be of American, European, or other descent. The preparations consist of a combination of bean sizes and shapes, with a specified maximum number of defects. The objective of this work was to evaluate the effect of the shape and size of the bean of the three main Catimors grown in Mexico, i.e., Colombia, Costa Rica, and Oro Azteca, on the cupping quality attributes of the beverage. The results showed that there was no significant difference between the cupping quality parameters of the Colombia and Costa Rica varieties, whereas Oro Azteca exhibited a final score significantly lower compared to those varieties. For beans of size 13/64 (Z13), significant differences were found between bean forms (flat, peaberry, and mixed) of the Colombia variety. Flat (FL) beans scored almost seven points higher in the final score compared to peaberries (PE). In coffee beans of size Z14 or Z15, mixed (MI) samples scored equal or significantly higher than FL samples in the three varieties. Small beans (Z13 and Z14) of samples not separated by shape (PE and FL together) obtained equal or significantly higher scores in the cup quality attributes of the beverage compared to bigger beans. It was demonstrated that the shape and size of the coffee bean significantly affect the cup quality parameters depending upon the variety.

IKLIM USAHA INDUSTRI PENGOLAHAN BIJI KAKAO

Indonesia is one among three major producers of cocoa beans. Nevertheless, industrial performance of cocoa beans industrial process bearish for the past few years. The purposes of this research are to analyse policies related to development the cocoa processing industry as well as analizing factor which cause low quality of cocoa beans. Factors that caused an uncondusif business climate on cocoa beans industry ar among of hers; high administrative fee in form of tax and entry charges for raw material that caused and increase in production cost structure of the industrial process of the cocoa beans, The low quality of the cocoa benas itself resulted from the un willingness of the farmers to ferment their products, ages of plantswhich more that its productive age and caused small beans producted, mixture between high quality and low quality beans and bugs infection of cocoa plants.

Efficiency of certain insecticides against the small bean beetle, Bruchidius incarnatus (Boh.).

Efficiency of certain insecticides against the small bean beetle, Bruchidius incarnatus (Boh.).

DETERMINATION OF THE REAL VALUES OF KINEMATIC INDEX CORRESPONDING TO THE MECHANICAL SEPARATION PROCESS

This article presents the methodology for determining the real values of kinematic indices which characterizes the mechanical separation of a mixture of solid particles. Mechanical separation of a mixture of solid-solid on surface provided with holes is the most common method of separation. To optimize equipment that performs this operation, both theoretical and practical characteristics are determined. Kinematic indices are part of the theoretical characteristics, helping to identify behavior of solid particles on a surface flat swing. Starting from an experimental batch, real values of the kinematic indices corresponding to up, down and sideways movement on the sieve were determined for the following types of real particles: grain, large grain beans, small grain beans and soybeans.

Effects of small black soybean product germinated with sulfur on immuno-redox status in C57BL/6 mice

While germinated soybean has been well known for higher content of bioactive substances, and peptides than regular soy bean, still low levels of sulfur containing amino acids to limit its nutritional values. To overcome this, a novel biofortified small black soybean sprout with the processed sulfur (GSSP) was prepared. Using this, we compared the immunomodulatory and anti-oxidant effects with other control groups (NT: small black soy bean non-treated/saline, NSP: normal non-germinated small black soybean product only, GSP: geminated small black soybean product) in C57BL/6 mice model orally fed at 400 or 800 mg/kg/day for two weeks. On immunomodulatory test, we found that GSSP led to the highest splenocyte proliferation as well as the lowest level of nitric oxide (NO) as an inflammatory mediator when compared to NT, NSP and GSP. In line, there was a significant increase of anti-inflammatory cytokine IL-10 level as well as a significant decrease of pro-inflammatory cytokine IL-6, TNF-α, and IFN-γ level in GSSP group when compared to other groups or NSP respectively. Next, redox finding was that with decrease of reactive oxygen species (ROS), there was a significant increase of sulfur-rich protein glutathione peroxidase (GPx) and catalase as endogenous antioxidants in GSP and GSSP group when compared to NT and NSP. Collectively, our study shows that biofortified small black soybean product with processed sulfur (GSSP) has the enhanced immunomodulatory, anti-oxidant effects via immunoredox balancing, importantly implying the promising candidate of soybean based-nutraceutical.

Fate of Anthocyanins through Cocoa Fermentation. Emergence of New Polyphenolic Dimers.

Fresh, ripe cocoa beans from Cameroon (German cocoa/Amelonado group and ICS 40/Trinitario group) were subjected to fermentation-like incubations in acetic acid, lactic acid, or both and to natural fermentation. Two naturally fermented samples from Cuba (UF 654/Trinitario group and C 411/Criollo group) were also investigated. Both cyanidin-3-galactoside and cyanidin-3-arabinoside (found as major anthocyanins in colored beans only) were drastically degraded through fermentation, especially in small beans and in the presence of acetic acid. On the other hand, emergence of a cyanidin-rhamnose isomer was evidenced, even in Criollo beans. In addition to the recently described structures F1 and F2 [m/z = 575 in ESI(-)], three additional polyphenolic structures [F3, F4, and F5; m/z = 557 in ESI(+)] were found after fermentation, the two former ones resulting from epicatechin oxidation. Synthesis of F5 requires an interclass reaction between cyani(di)n and epicatechin, which explains its absence in fermented Criollo beans.

Inhibitory Effects on Human Breast Cancer Cells Migration of Small Black Bean according to the Cooking Methods

Inhibitory Effects on Human Breast Cancer Cells Migration of Small Black Bean according to the Cooking Methods

Susceptibility to bruchids among common beans in Uganda

The bean bruchids, Acanthoscelides obtectus Say and Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae), are cosmopolitan pests of stored dry common beans ( Phaseolus vulgaris L.), causing damage through reduction of grain quality and seed germination. Biological resistance to these bruchids was definitively established in noncultivated bean accessions, and has been introgressed into a range of drybean market classes. However, existing resistance to bruchids in Uganda’s common bean germplasm has not been systematically studied. In this study, 45 bean genotypes from the National Bean-Breeding Programme (25 genotypes) and agroecologically diverse bean growing areas in Uganda (20 genotypes), were evaluated for postharvest bruchid resistance. None of the evaluated bean genotypes expressed resistance to either bruchid species, with all the 45 bean genotypes supporting bruchid development, reproduction and feeding. All genotypes were severely damaged by bruchids feeding, resulting in significant (P<0.05) reduction of seed germination. Reduction in seed germination was related to the number of emergence holes and seed size; small bean seeds damaged by up to 2 bruchid emergence holes had a 7.1% reduction in germination, while large bean seeds with a similar number of emergence holes showed a 25% reduction in germination. Whereas this study further confirms bruchids as important storage pests of beans causing direct loss through consumption of the seed and indirect loss through viability deterioration, the resistance to bruchids in the evaluated range of Uganda’s dry bean germplasm is inadequate for direct exploitation in a breeding programme. Key Words : Acanthoscelides obtectus, Phaseolus vulgaris, Zabrotes subfasciatus

DETERMINATION OF THE REAL VALUES OF KINEMATIC INDEX CORRESPONDING TO THE MECHANICAL SEPARATION PROCESS

This article presents the methodology for determining the real values of kinematic indices which characterizes the mechanical separation of a mixture of solid particles. Mechanical separation of a mixture of solid-solid on surface provided with holes is the most common method of separation. To optimize equipment that performs this operation, both theoretical and practical characteristics are determined. Kinematic indices are part of the theoretical characteristics, helping to identify behavior of solid particles on a surface flat swing. Starting from an experimental batch, real values of the kinematic indices corresponding to up, down and sideways movement on the sieve were determined for the following types of real particles: grain, large grain beans, small grain beans and soybeans.