Abstract

The pasting profile of starchy tissues is conventionally measured by recording the apparent viscosity (η) in heating/cooling cycles. However, conventional rheometers show critical limitations when the starch is embedded in compact protein-rich cotyledon matrices, as occurs in pulses. In this work, the pasting profile of 13 red kidney beans (Phaseolus vulgaris) from the same cultivar but different growing locations was investigated using a heating/cooling cycle at higher temperature (130 °C) and pressurized conditions, using both water and brine as cooking solvents. It was hypothesized that the continuous measure of η at these conditions of flours from the dry seed would correlate with the texture, as determined by the mini-Kramer cell, of the beans after the entire process of soaking and canning. Furthermore, mechanistic answers were obtained by investigating their composition (starch, protein, and ash content) and physical properties (water holding capacity, seed ratio and weight). Interestingly, as opposed to the pasting profile at 95 °C, pasting indicators at 130 °C, including trough and final viscosity, strongly correlated with starch and protein content, seed coat ratio and, remarkably, with the firmness of the beans after canning when brine was incorporated. These results clearly show that small beans with a high protein content would bring about a more compact matrix that restricts starch from swelling and results in canned beans with a hard texture, which can be predicted by a lower pasting profile of the whole bean flour.

Highlights

  • The demand for plant-based proteins is rapidly growing with increased awareness of the carbon footprint caused by a meat-based diet

  • To obtain whole bean flours for analyses, beans were cryo-milled for 2 min in a coffee grinder (Black+Decker CBG100SC, Middleton, WI, USA) and sifted under a 250-micrometer mesh sieve (Retsch AS 200, Haan, Germany)

  • The ground whole flours were stored in a freezer at −20 ◦ C until further analyses in order to minimize any enzymatic activity in the sample

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Summary

Introduction

The demand for plant-based proteins is rapidly growing with increased awareness of the carbon footprint caused by a meat-based diet. The consumption of pulses has been associated with a reduced prevalence of type 2 diabetes and cardiovascular disease [4]. Abdullah et al [4] estimated that an increased consumption of pulses to 100 g/day per person could save up to CAD USD 315 million on healthcare costs annually in Canada, attributed to anticipated reduced incidence of type 2 diabetes, colon cancer, and cardiovascular disease. Pulse consumption still remains low in Canada, with only 13% of the adult population reporting the consumption of pulses (at an average of 113 g) on any given day [5]

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