Allergic Skin Diseases Research Articles

Therapeutic action of Kushen recipe extractive and its inhibitory effect on eotaxin in mouse models with contact dermatitis.

Treatment of skin allergic diseases remains a challenging research topic. To investigate the effect of Kushen recipe extractive (KS) gel on contact dermatitis (CD) of mouse. Allergic contact dermatitis (ACD) model of mouse was established. Immunohistochemical method (ICH) and flow cytometry method (FCM) were used to detect CD4+ and CD8+ T lymphocytes and explore the regulation effect of KS on the immune status of the organism. The expression status of eotaxin tissue was evaluated by real-time polymerase chain reaction (RT-PCR), ICH, and western blotting method. The survival rates of HaCaT cell and Fibroblasts affected by KS were detected by methyl thiazolyl tetrazolium (MTT) method. The inhibitory effect of KS on eotaxin produced by HaCaT cell and FBs induced by TNF-α and interleukin (IL)-4 were evaluated using RT-PCR and enzyme-linked immunosorbent assay methods. The inhibitory effect of KS on nuclear factor-κB (NF-κB) and Signal transducers and activators of transcription 6 (STAT6) activation induced by TNF-α and IL-4 was detected by electrophoretic mobility shift assay and western blotting methods. We confirmed that KS shows favorable therapeutic effect on CD, which can obviously inhibit eotaxin expression and Eosinophils recruitment in allergic skin of mouse, as well as regulate the immune status of the organism. Furthermore, KS and its main effective components can inhibit TNF-α and IL-4 induced upregulation of eotaxin via the two signal transduction pathways, NF-κB and STAT6. The great importance of traditional Chinese recipe KS is evidenced by its therapeutic effect and mechanism in ACD of mouse.

Efficacy and tolerance of a novel topical TRPV-1 channel antagonist in dogs with allergic pododermatitis.

Pruritus due to allergic skin disease is one of the most common reasons for dermatological consultations in the veterinary clinic. Treatment is usually multimodal and requires continuous monitoring and reassessment. New therapies are needed to broaden the therapeutic arsenal. The aim of this study was to evaluate the efficacy of a novel transient receptor potential vanilloid 1 (TRPV1) channel antagonist for allergic pododermatitis in dogs. Twenty-four client-owned dogs with allergic pododermatitis. The study was an open, prospective, multi-centreclinical trial with client-owned dogs. All dogs were treated twice daily with a spray containing hydroxymethoxyiodobenzyl glycolamide pelargonate for 28 days. Clinical assessments included pruritus Visual Analog Scale (PVAS), pedal skin lesion score, evaluation of quality of life (QoL), presence of secondary infections and a four-point subjective efficacy assessment by the veterinarian and the dog owner. There was more than 50% improvement in all scores by the conclusion of the study. Secondary infections were reduced (p < 0.001). Both the veterinarians and dog owners evaluated the efficacy of the product positively. The product was well-tolerated. This study demonstrated the tolerability and efficacy of a TRPV1 antagonist on pruritic pododermatitis in 24 dogs.

Transcription of interleukin 31 and its receptor by leukocytes after Culicoides sp stimulation is dose dependent but is not exaggerated in allergic horses or correlated with pruritus.

Preliminary evidence supports a role for IL-31 in equine insect bite hypersensitivity (IBH) and pruritus. Our studies investigated IL-31 and IL-31 receptor-α (IL-31RA) transcription in leukocytes from normal and IBH horses in response to Culicoides nubeculosus. 19 normal and 15 IBH horses were recruited in the summer of 2019 (low-dose study) and 8 normal and 10 IBH horses in the winter of 2022 to 2023 (high-dose study). Normal horses had no history or signs of allergic skin disease, while IBH horses had a history and clinical signs compatible with IBH. Pruritus was scored using a visual analog score or a 1 to 6 grading system. Whole blood leukocytes were incubated with saline (0.9% NaCl) solution or C nubeculosus (0.26 µg/mL [low dose]; 5 μg/mL [high dose]). Transcription of IL-31 and IL-31RA was measured by quantitative RT-PCR. Transcription of IL-31 and IL-31RA significantly increased in leukocytes from normal and IBH horses following high-dose C nubeculosus, and no differences were found between populations. Following low-dose C nubeculosus IL-31RA, transcription was increased in both normal and IBH horses, but IL-31 transcription was reduced in normal horses. No positive correlation was found between pruritus scores and IL-31 transcription after low- or high-dose C nubeculosus stimulation. Exaggerated IL-31 transcription was not identified in IBH horses, suggesting that dysregulation in IL-31 signaling occurs downstream or in localized tissues or involves regulation by yet unidentified receptor splice variants or IL-31-induced increased sensitivity to other pruritogens. Further studies to understand IL-31 signaling in equine allergic skin disease are needed.

The Quality of Life of Allergic Cats, Treated with Allergen-Specific Immunotherapy – A Retrospective Study

Abstract Feline atopic skin syndrome (FASS) describes allergic skin diseases in cats associated with sensitivity to environmental allergens. Allergen-specific immunotherapy (ASIT) aims to reduce or eliminate symptoms associated with subsequent exposure to the causative allergen. The primary outcome of this study was to determine if the quality of life (QoL) of cats and owners improved as a result of ASIT. The secondary outcome was to determine whether the QoL improved in cats treated with ASIT compared with cats treated symptomatically. Eight cats were included in the ASIT group (AG) and 6 cats in the control group (CG). Validated quality of life questionnaires were retrospectively completed by cat owners for the period before and after treatment. The QoL of cats improved significantly with ASIT. Cats were significantly less lazy, nervous, aggressive, restrained, preened less while sleeping and hid less, were less disturbed by consultations, were more playful and interactive with their environment, and had better appetites than before treatment. Owners felt significantly less physically exhausted and less emotionally distressed compared to before ASIT treatment and their expenses were significantly lower. On the contrary various symptomatic treatments did not significantly improve the quality of life of the cats or their owners in any of the terms. No serious side effects were observed with ASIT. The quality of life of animals suffering from chronic diseases is important as it determines the decisions of owners and veterinarians on the modalities of lifelong treatment. Validated questionnaires are still rarely used in veterinary practice and research.

Equine allergic skin diseases: Clinical consensus guidelines of the World Association for Veterinary Dermatology.

Allergic skin diseases are common in horses worldwide. The most common causes are insect bites and environmental allergens. To review the current literature and provide consensus on pathogenesis, diagnosis, treatment and prevention. The authors reviewed the literature up to November 2022. Results were presented at North America Veterinary Dermatology Forum (2021) and European Veterinary Dermatology Congress (2021). The report was available to member organisations of the World Association for Veterinary Dermatology for feedback. Insect bite hypersensitivity (IBH) is the best characterised allergic skin disease. An immunoglobulin (Ig)E response against Culicoides salivary antigens is widely documented. Genetics and environmental factors play important roles. Tests with high sensitivity and specificity are lacking, and diagnosis of IBH is based on clinical signs, seasonality and response to insect control. Eosinophils, interleukin (IL)-5 and IL-31 are explored as therapeutic targets. Presently, the most effective treatment is insect avoidance. Existing evidence does not support allergen-specific immunotherapy (ASIT) using commercially available extracts of Culicoides. Hypersensitivity to environmental allergens (atopic dermatitis) is the next most common allergy. A role for IgE is supported by serological investigation, skin test studies and positive response to ASIT. Prospective, controlled, randomised studies are limited, and treatment relies largely on glucocorticoids, antihistamines and ASIT based on retrospective studies. Foods are known triggers for urticaria, yet their role in pruritic dermatitis is unknown. Recurrent urticaria is common in horses, yet our understanding is limited and focussed on IgE and T-helper 2 cell response. Prospective, controlled studies on treatments for urticaria are lacking. Glucocorticoids and antihistamines are primary reported treatments.

Functional heterogeneity of human skin-resident memory T cells in health and disease.

The human skin is populated by a diverse pool of memory T cells, which can act rapidly in response to pathogens and cancer antigens. Tissue-resident memory T cells (TRM ) have been implicated in range of allergic, autoimmune and inflammatory skin diseases. Clonal expansion of cells with TRM properties is also known to contribute to cutaneous T-cell lymphoma. Here, we review the heterogeneous phenotypes, transcriptional programs, and effector functions of skin TRM . We summarize recent studies on TRM formation, longevity, plasticity, and retrograde migration and contextualize the findings to skin TRM and their role in maintaining skin homeostasis and altered functions in skin disease.

Hypoxia-inducible factor prolyl hydroxylase inhibitors suppressed thymic stromal lymphopoietin production and allergic responses in a mouse air-pouch-type ovalbumin sensitization model.

Atopic dermatitis (AD) is an allergic skin disease, triggered by excessive type 2 immune reactions. Thymic stromal lymphopoietin (TSLP) is an epithelial-derived cytokine that induces type 2 immune response through dendritic cell activation. Therefore, TSLP inhibitors may serve as novel antiallergic drugs. Hypoxia-inducible factor (HIF) activation in the epithelia contributes to several homeostatic phenomena, such as re-epithelialization. However, the effects of HIF activation on TSLP production and immune activation in the skin remain unclear. In this study, we found that selective HIF prolyl hydroxylase inhibitors (PHD inhibitors), which induce HIF activation, suppressed TSLP production in a mouse ovalbumin (OVA) sensitization model. PHD inhibitors also suppressed the production of tumor necrosis factor-alpha (TNF-α), which is a major inducer of TSLP production, in this mouse model and in a macrophage cell line. Consistent with these findings, PHD inhibitors suppressed OVA-specific IgE levels in the serum and OVA-induced allergic responses. Furthermore, we found a direct suppressive effect on TSLP expression in a human keratinocyte cell line mediated by HIF activation. Taken together, our findings suggest that PHD inhibitors exert antiallergic effects by suppressing TSLP production. Controlling the HIF activation system has therapeutic potential in AD.

Evaluation of clinicoepidemiological features of pediatric dermatoses in Egyptian children

Background Epidemiological studies determine the burden of skin diseases, especially in children, which is required for proper health-care planning. Objectives To evaluate the clinicoepidemiologic features of skin diseases among Egyptian children. Patients and methods This was a cross-sectional, community-based study that included 2594 children of both sexes with ages that ranged from birth to less than 18 years. This study included government schools, private schools, and maternity and childhood care centers in Sohag Governorate, Upper Egypt. Sociodemographic data, the general examination included body parameters, and dermatological examination of children were documented. Results Skin diseases were detected in 25.1% (651/2594) of the examined children. Allergic skin diseases were the most common detected, one in 32.4% of children followed by sebaceous gland disorders (acne vulgaris) in 30.7% and then infectious skin diseases in 24.85%. Head was affected in the majority of patients (54.8%). The median age of children with skin diseases was 11 (1–18) years. Females constituted 66% of children with skin diseases. Children with skin diseases from rural areas constituted 63.1%. The median age of children with an infectious skin disease was 8 (2–14) years. Female sex, rural residence, and parents of low educational levels were associated with higher percentages of infectious skin diseases in children. Conclusion This study concluded that the most common detected pediatric skin diseases were allergic skin disorders, sebaceous gland disorders, and infectious skin diseases, respectively. Socioeconomic status, environmental factors, and medical resources are the most important factors affecting the prevalence of skin diseases.

BLOC1S1: An Unexpected Regulator of Th2 Cell-driven Inflammatory Responses

Abstract BLOC1S1 is an adaptor protein that modulates endosome-lysosome function as a component of numerous multiprotein complexes. We postulated that its modulation in CD4 +T cells may help us uncover the role of endosome-lysosome biology in adaptive immunity. In primary CD4 +T cells BLOC1S1 cre-recombinase knockout resulted in the preferential induction of Th2 compared to Th1 and Th17 cytokine production following T cell receptor activation. As atopic dermatitis is an allergic inflammatory skin disease characterized by the production of the type 2 cytokines in the skin we evaluated the effect of CD4 +T cell BLOC1S1 KO vs controls in calcipotriol-induced atopic dermatitis in mice. The absence of BLOC1S1 exhibited exacerbated atopic dermatitis accompanied with significantly elevated plasma IgE, skin eosinophilia, elevated type 2 responses, and inflammatory pathology relative to wild-type mice. The transcription factor Gata-3 is expressed in Th2 but not Th1 cells and plays a critical role in Th2 differentiation and atopic dermatitis. BLOC1S1 deficient CD4 +T cells showed enhanced nuclear factor kB (NF-kB), Gata3 and LC3 positive autophagosomes. Inhibition of NF-kB activity prevented Gata-3 expression, LC3 positive autophagosomes and Th2 cytokine production in BLOC1S1 −/− CD4 +T cells. This mouse model will now give us a platform to explore the role of endo-lysosomal biology in Th2 polarization and activation. NHLBI Division of Intramural Research (MNS-ZIA-HL005102)

Serological analysis of allergic components of house dust mite provides more insight in epidemiological characteristics and clinical symptom development in North China.

House dust mite (HDM) is the most common airborne source causing complex allergy symptoms. There are geographic differences in the allergen molecule sensitization profiles. Serological testing with allergen components may provide more clues for diagnosis and clinical management. This study aims to investigate the sensitization profile of eight HDM allergen components in a large number of patients enrolled in the clinic and to analyze the relation of gender, age, and clinical symptoms in North China. The 548 serum samples of HDM-allergic patients (ImmunoCAP® d1 or d2 IgE ≥0.35) were collected in Beijing City and divided in four different age groups and three allergic symptoms. The specific IgE of HDM allergenic components, Der p 1/Der f 1, Der p 2/Der f 2, Der p 7, Der p 10, Der p 21, and Der p 23, was measured using the micro-arrayed allergen test kit developed by Hangzhou Zheda Dixun Biological Gene Engineering Co., Ltd. The new system was validated by comparing to single-component Der p 1, Der p 2, and Der p 23 tests by ImmunoCAP in 39 sera. The epidemiological study of these IgE profiles and the relation to age and clinical phenotypes were analyzed. A greater proportion of male patients was in the younger age groups, while more female patients were in the adult groups. Both the sIgE levels and the positive rates (approximately 60%) against Der p 1/Der f 1 and Der p 2/Der f 2 were higher than for the Der p 7, Der p 10, and Der p 21 components (below 25%). The Der f 1 and Der p 2 positive rates were higher in 2-12-year-old children. The Der p 2 and Der f 2 IgE levels and positive rates were higher in the allergic rhinitis group. The positive rates of Der p 10 increased significantly with age. Der p 21 is relevant in allergic dermatitis symptom, while Der p 23 contributes to asthma development. HDM groups 1 and 2 were the major sensitizing allergens, with group 2 being the most important component relevant to respiratory symptoms in North China. The Der p 10 sensitization tends to increase with age. Der p 21 and Der p 23 might be associated with the development of allergic skin disease and asthma, respectively. Multiple allergen sensitizations increased the risk of allergic asthma.

Pathogenic role of the staphylococcal accessory gene regulator quorum sensing system in atopic dermatitis.

The skin is home to various bacteria, archaea, fungi, and viruses, collectively referred to as the skin microbiota. Patients with certain skin diseases reportedly have unique skin "dysbiosis," a condition involving imbalanced microbiota, suggesting that dysbiosis in the skin may be either causal or a consequence of specific skin diseases. Atopic dermatitis (AD) is the most common allergic skin disease that affects 15-20% of children and 2-10% of adults worldwide. Both intrinsic genetic factors, such as susceptibility to type 2 inflammation or skin barrier dysfunction, and extrinsic environmental factors, such as air pollen and skin microbiota, contribute to AD. Staphylococcus aureus, which does not often colonize the skin of healthy individuals, is commonly identified in the lesional skin of patients with AD and is correlated with the disease flare. However, the role of S. aureus in the pathogenesis of AD has not been elucidated. Here, we discuss the pathological behavior of S. aureus, focusing on accessory gene regulator (Agr) quorum sensing, which is a fundamental bacterial cell-to-cell interaction mechanism that affects the behavior of S. aureus and other members of the microbial community. Importantly, beyond bacteria-bacteria interactions, the Agr quorum sensing system also regulates various virulence factors, which induce type 2 and IL-17-dependent skin inflammation in the host. Furthermore, the colonization of Agr-positive S. aureus in early life accelerates the development of pediatric AD. Finally, we aim to highlight the current efforts to establish novel therapeutic methods to ameliorate or prevent AD through Agr-targeted intervention.

The Effect of Insect Bite Hypersensitivity on Movement Activity and Behaviour of the Horse.

Insect bite hypersensitivity (IBH) associated with Culicoides biting midges is a common allergic skin disease in horses, reducing the welfare of affected horses. This study investigated the effect of IBH on animal welfare and behaviour and assessed a new prophylactic insect repellent. In total, 30 horses were recruited for a prospective cross-over and case-control study. Clinical signs of IBH, inflammatory markers in skin biopsies and behavioural data (direct observations, motion index) were scored longitudinally during two consecutive summers. No differences were observed in the total number of itching behaviours or motion index between IBH-affected horses and controls, but higher numbers of itching behaviours were observed in the evening. IBH-affected horses showed both clinical and histopathological signs of inflammatory skin lesions, with even short periods of scratching being associated with moderate/severe inflammatory skin lesions. In order to improve the welfare of the IBH-affected horses, they should be stabled/given extra protection in the evening and even short-term exposure to Culicoides should be avoided. Preliminary results showed that the repellent tested can be used as a safe and non-toxic prophylactic to potentially reduce allergen exposure in horses with IBH, but further studies are needed to determine its efficacy.

Oclacitinib 10 years later: lessons learned and directions for the future

Oclacitinib was approved in the United States 10 years ago for the management of atopic dermatitis (AD) and allergic skin disease in dogs. Many studies and case reports have been published in the past 10 years on the efficacy and safety of this medication, both at labeled doses to treat allergic dogs and off label to treat other diseases and given to other species. Concerns and confusion have occurred for both clinicians and owners regarding the long-term safety of this drug. The purpose of this review is to present the current knowledge on the efficacy, speed of action, effects on the immune system, and clinical safety of oclacitinib, based on evidence and published literature. We also aim to summarize the lessons learned in the past 10 years and to propose directions for the future.

Development of an inhibiting antibody against equine interleukin 5 to treat insect bite hypersensitivity of horses

Insect bite hypersensitivity (IBH) is the most common allergic skin disease of horses. It is caused by insect bites of the Culicoides spp. which mediate a type I/IVb allergy with strong involvement of eosinophil cells. No specific treatment option is available so far. One concept could be the use of a therapeutic antibody targeting equine interleukin 5, the main activator and regulator of eosinophils. Therefore, antibodies were selected by phage display using the naïve human antibody gene libraries HAL9/10, tested in a cellular in vitro inhibition assay and subjected to an in vitro affinity maturation. In total, 28 antibodies were selected by phage display out of which eleven have been found to be inhibiting in the final format as chimeric immunoglobulin G with equine constant domains. The two most promising candidates were further improved by in vitro affinity maturation up to factor 2.5 regarding their binding activity and up to factor 2.0 regarding their inhibition effect. The final antibody named NOL226-2-D10 showed a strong inhibition of the interleukin 5 binding to its receptor (IC50 = 4 nM). Furthermore, a nanomolar binding activity (EC50 = 8.8 nM), stable behavior and satisfactory producibility were demonstrated. This antibody is an excellent candidate for in vivo studies for the treatment of equine IBH.

Dermatophagoides farinae Extract Induces Interleukin 33-Mediated Atopic Skin Inflammation via Activation of RIP1

Receptor-interacting protein kinase (RIP) family 1 signaling has complex effects on inflammatory processes and cell death, but little is known concerning allergic skin diseases. We examined the role of RIP1 in Dermatophagoides farinae extract (DFE)-induced atopic dermatitis (AD)-like skin inflammation. RIP1 phosphorylation was increased in HKCs treated with DFE. Nectostatin-1, a selective and potent allosteric inhibitor of RIP1, inhibited AD-like skin inflammation and the expression of histamine, total IgE, DFE-specific IgE, IL-4, IL-5, and IL-13 in an AD-like mouse model. The expression of RIP1 was increased in ear skin tissue from a DFE-induced mouse model with AD-like skin lesions and in the lesional skin of AD patients with high house dust mite sensitization. The expression of IL-33 was down-regulated after RIP1 inhibition, and the levels of IL-33 were increased by over-expression of RIP1 in keratinocytes stimulated with DFE. Nectostatin-1 reduced IL-33 expression in vitro and in the DFE-induced mouse model. These results suggest that RIP1 can be one of the mediators that regulate IL-33-mediated atopic skin inflammation by house dust mites.

Depletion of T and B cells in lymphoid tissues of mice induced by oclacitinib, a Janus kinase inhibitor.

The main purpose of the study was to determine the safety of oclacitinib (OCL), a Janus kinase inhibitor, with respect of its effect on CD4 + and CD8 + T cells as well as B cells in the lymphoid tissue. The mice were treated orally with OCL at a dose of 2.7 mg/kg for 14 days and peripheral blood, head and neck lymph nodes (HNLNs), mediastinal lymph nodes (MLNs) and spleen were collected. The study found that OCL induced depletion of CD4 + T cells in the HNLNs and MLNs, while it did not affect the absolute count of CD8 + T cells in these tissues. Also OCL caused a loss of B cells in the HNLNs, although not in the MLNs. Moreover, OCL depleted B cells in the peripheral blood, but did not affect the absolute count of CD4 + and CD8 + T cells. Thus, it can be concluded that OCL may induce a depletive effect on CD4 + and CD8 + T cells as well as B cells in the lymphoid tissue. This effect should be seen as an unfavorable one, especially in patients with infections. Therefore, a clinical implication is that in such patients, the benefit/risk ratio should be thoroughly considered by clinicians. Moreover, OCL reduced the absolute count of eosinophils, basophils, neutrophils and monocytes. However, it is uncertain whether this effect should be considered to be of clinical importance because the levels of these cells were within the physiological range. It is possible that the depletive effect of OCL toward T and B cells, as well as eosinophils and basophils may contribute to the beneficial effects of the drug in the treatment of skin allergic diseases.

Crosstalk between Aryl Hydrocarbon Receptor (AHR) Pathway and IL-13 Signaling in Human Keratinocytes and Implications for Atopic Dermatitis

Atopic dermatitis (AD) is an allergic skin disease mediated by skin barrier dysfunction and an IL-13-driven immune response. The aryl hydrocarbon receptor (AHR) is a sensor molecule that is activated by numerus environmental ligands, some of which have shown promise in clinical trials of AD. Therefore, we hypothesized that the AHR pathway can attenuate IL-13-induced responses.

Ozonated oil alleviates dinitrochlorobenzene-induced allergic contact dermatitis via inhibiting the FcεRI/Syk signaling pathway.

Ozone is widely applied to treat allergic skin diseases such as eczema, atopic dermatitis, and contact dermatitis. However, the specific mechanism remains unclear. This study aims to investigate the effects of ozonated oil on treating 2,4-dinitrochlorobenzene (DNCB)-induced allergic contact dermatitis (ACD) and the underling mechanisms. Besides the blank control (Ctrl) group, all other mice were treated with DNCB to establish an ACD-like mouse model and were randomized into following groups: a model group, a basal oil group, an ozonated oil group, a FcεRI-overexpressed plasmid (FcεRI-OE) group, and a FcεRI empty plasmid (FcεRI-NC) group. The basal oil group and the ozonated oil group were treated with basal oil and ozonated oil, respectively. The FcεRI-OE group and the FcεRI-NC group were intradermally injected 25 µg FcεRI overexpression plasmid and 25 µg FcεRI empty plasmid when treating with ozonated oil, respectively. We recorded skin lesions daily and used reflectance confocal microscope (RCM) to evaluate thickness and inflammatory changes of skin lesions. Hematoxylin-eosin (HE) staining, real-time PCR, RNA-sequencing (RNA-seq), and immunohistochemistry were performed to detct and analyze the skin lesions. Ozonated oil significantly alleviated DNCB-induced ACD-like dermatitis and reduced the expressions of IFN-γ, IL-17A, IL-1β, TNF-α, and other related inflammatory factors (all P<0.05). RNA-seq analysis revealed that ozonated oil significantly inhibited the activation of the DNCB-induced FcεRI/Syk signaling pathway, confirmed by real-time PCR and immunohistochemistry (all P<0.05). Compared with the ozonated oil group and the FcεRI-NC group, the mRNA expression levels of IFN-γ, IL-17A, IL-1β, IL-6, TNF-α, and other inflammatory genes in the FcεRI-OE group were significantly increased (all P<0.05), and the mRNA and protein expression levels of FcεRI and Syk were significantly elevated in the FcεRI-OE group as well (all P<0.05). Ozonated oil significantly improves ACD-like dermatitis and alleviated DNCB-induced ACD-like dermatitis via inhibiting the FcεRI/Syk signaling pathway.

Global Research Trend on Allergic Skin Disorders: A Bibliometric Analysis from 2001 to 2020.

Allergic skin disorders constitute a variety of inflammatory skin disorders with increasing incidence. Bibliometric studies involve a statistical analysis of academic literature to assess the current research trend and identify knowledge gaps. There is a dearth of such studies concerning allergic skin disorders. To perform a bibliometric analysis of global research concerning allergic skin disorders from 2001 to 2020. We obtained all data from the Web of Science using the keywords "atopic dermatitis," "contact dermatitis," "skin allergy," "urticaria," "food allergy," and "drug allergy." Only articles in English language were included. Subsequent analysis revealed the total number of publications, top journals, institutions, and countries, thus highlighting the overall research trend. Overall 76,764 articles were published on allergic skin disorders from 2001 to 2020 (original articles > review articles). The United States of America (USA) contributed maximum publications (26.1%) followed by Germany (9.6%), Japan (8.2%), and England (8.1%). The Allergy is the most preferred journal for publishing skin allergy research. Most research concentrated on atopic dermatitis, pathomechanisms of allergic disorders, and their primary prevention. This study evaluates the current landscape of skin allergy research. There has been a consistent increase in the number of publications concerning allergic skin disorders over the years. However, majority of the research publications are from developed countries. Hence, skin allergy-related research publication should be increased for diverse and enriched literary evidences.

Achados clínicos e epidemiológicos e impacto do estilo de vida na dermatite atópica canina em Fortaleza/Brasil

ABSTRACT: Canine atopic dermatitis (cAD) is a worldwide allergic skin disease. The affected dog population can show different clinical patterns according to geographic region, and a lack of studies in Brazil is observed. Therefore, the aim of the present study was to assess the clinical and epidemiological data of cAD in dogs treated in a private clinical practice in Fortaleza, a city located in the Northeast Region of Brazil. cAD was diagnosed in 35% of dogs, being Shih-tzu and Poodle the most affected breeds. Paws and ears were frequently injured sites. Almost 50% of atopic dogs were diagnosed with superficial pyoderma and 36% with cutaneous malasseziosis. Atopic dogs with outdoor habits were less likely to develop cutaneous malassezial infection, and with routine ear, cleaning habits were less likely to develop bacterial otitis externa. In conclusion, canine atopic dermatitis is a prevalent disease in private clinical practice in Fortaleza, and lifestyle habits can be considered a risk factor for cutaneous malasseziosis infection and bacterial otitis externa in atopic dogs.