Abstract The authors evaluate the skill of a suite of seasonal hydrological prediction experiments over 28 watersheds throughout the southeastern United States (SEUS), including Florida, Georgia, Alabama, South Carolina, and North Carolina. The seasonal climate retrospective forecasts [the Florida Climate Institute–Florida State University Seasonal Hindcasts at 50-km resolution (FISH50)] is initialized in June and integrated through November of each year from 1982 through 2001. Each seasonal climate forecast has six ensemble members. An earlier study showed that FISH50 represents state-of-the-art seasonal climate prediction skill for the summer and fall seasons, especially in the subtropical and higher latitudes. The retrospective prediction of streamflow is based on multiple calibrated rainfall–runoff models. The hydrological models are forced with rainfall from FISH50, (quantile based) bias-corrected FISH50 rainfall (FISH50_BC), and resampled historical rainfall observations based on matching observed analogs of forecasted quartile seasonal rainfall anomalies (FISH50_Resamp). The results show that direct use of output from the climate model (FISH50) results in huge biases in predicted streamflow, which is significantly reduced with bias correction (FISH50_BC) or by FISH50_Resamp. On a discouraging note, the authors find that the deterministic skill of retrospective streamflow prediction as measured by the normalized root-mean-square error is poor compared to the climatological forecast irrespective of how FISH50 (e.g., FISH50_BC, FISH50_Resamp) is used to force the hydrological models. However, our analysis of probabilistic skill from the same suite of retrospective prediction experiments reveals that, over the majority of the 28 watersheds in the SEUS, significantly higher probabilistic skill than climatological forecast of streamflow can be harvested for the wet/dry seasonal anomalies (i.e., extreme quartiles) using FISH50_Resamp as the forcing. The authors contend that, given the nature of the relatively low climate predictability over the SEUS, high deterministic hydrological prediction skills will be elusive. Therefore, probabilistic hydrological prediction for the SEUS watersheds is very appealing, especially with the current capability of generating a comparatively huge ensemble of seasonal hydrological predictions for each watershed and for each season, which offers a robust estimate of associated forecast uncertainty.