Reduction In Size Research Articles

In Vivo Evaluation of Sepigel-Based Meglumine Antimoniate and Amphotericin B for Cutaneous Leishmaniasis Treatment.

Cutaneous leishmaniasis (CL) poses a significant public health concern in endemic regions due to its increasing prevalence and substantial impact on affected individuals. This disease is primarily caused by the Leishmania protozoa, which are transmitted through insect bites, and it manifests as a range of symptoms, from self-healing lesions to severe disfigurement. Current treatments, which often involve the parenteral administration of antimonials, face challenges such as poor compliance and adverse effects. This study investigates the efficacy of topical formulations containing meglumine antimoniate (MA) and amphotericin B (AmB), using Sepigel as an excipient, for treating CL. In the in vivo study, BALB/c mice infected with L. amazonensis developed lesions at the injection site five weeks post-infection. Subsequently, the mice were divided into eight groups: untreated mice, mice treated orally with miltefosine, mice treated intraperitoneally with MA, and mice treated topically with 15%, 22.5%, and 30% MA-Sepigel, as well as those treated with AmB-Sepigel. Treatments were applied daily for two weeks, and the results revealed a significant reduction in lesion size and parasite burden following topical application, particularly with the AmB-Sepigel formulations and 30% MA-Sepigel. Additionally, Sepigel-based treatments demonstrated improved patient compliance and reduced toxicity compared to systemic therapies. These findings underscore the potential of Sepigel-based formulations as a promising alternative for CL treatment. They offer enhanced efficacy and tolerability, while reducing the systemic toxicity associated with conventional therapies.

Study of the stability of model emulsions mimicking petroleum with different types of non-ionic surfactants

This study conducted experiments to mimic petroleum emulsions for application in laboratory flow circuits. The science of emulsion formulation is still quite restricted when it comes to parameters that stabilize emulsions. The challenge is even greater when formulating emulsions of low dynamic viscosity. In this work, model emulsions were prepared with different oil phases, with 0.1 and 1.0 % v/v of the surfactants Span 60, Span 80, Triton X-100, and Triton X-114, with 10 and 30 % v/v of aqueous phase. The kinetic stability of the emulsions evaluated in terms of aqueous phase separation, droplet size distribution, dynamic viscosity, and interfacial tension. The homogenization process assessed to identify the emulsification regime of the emulsions, inertial or viscous, through the calculation of the smallest vortices formed. A study of the maximum superficial flow velocity conducted to provide users with a better understanding of the emulsions produced here. The results indicate that seven emulsions can used in laboratory flow circuits. Span 80 provided better stabilization of the emulsions for over 72 hours with droplet sizes in the range of 0.2–100.0 µm. As a novelty in this work, increasing the concentration of surfactant Span 80 causes a decrease in average velocity in flow, a reduction in droplet size, and a regime of turbulent viscous emulsification in axial flow.

TREO Aortic Endograft Demonstrates Superior Aneurysmal Sac Regression Over Mid-Term Follow-up

IntroductionAneurysmal sac regression is a predictor of long-term outcomes after endovascular aneurysm repair (EVAR). This study aimed to compare a large cohort of TREO and non-TREO endografts over a mid-term follow-up and compare abdominal aortic aneurysm sac regression. The hypothesis was that TREO endografts have an increased sac regression by 24 mo. MethodsThis is a retrospective analysis of all EVARs completed at a single institution between 2015 and 2024. Clinical and imaging data were collected from an institutional database and patients’ records. The analysis included all TREO and age, sex, anticoagulation use and current smoking-matched non-TREO endografts that satisfied anatomic indications for use of the TREO graft. The primary outcomes were sac regression at 12 and 24 mo, and secondary outcomes were rates of mortality, endoleak, and reintervention. ResultsTwenty-one TREO grafts were matched to 68 non-TREO grafts. The groups were similar in demographics, comorbidities, and preoperative anatomy. Preoperative abdominal aortic aneurysm sac size was larger in the TREO cohort. The mean reduction in sac size in mm was greater in the TREO cohort compared to the non-TREO cohort (−12.6 ± 8.95 versus −7.83 ± 7.74 mm, P = 0.039) over the study period. Cox regression analysis identified the TREO stent graft to be associated with 1-y sac regression (hazard ratio = 2.42, P = 0.019). The incidence of all-cause endoleaks, reintervention, and mortality were similar between cohorts. ConclusionsThese findings suggest that the TREO endograft offers better mid-term outcomes with respect to sac regression with no differences in the incidence of endoleak, mortality, or reintervention.

Perioperative Predictive Factors for Tumor Regression and Survival in Non-Small Cell Lung Cancer Patients Undergoing Neoadjuvant Treatment and Lung Resection.

Our study aimed to identify predictors for the effectiveness of tumor regression in lung cancer patients undergoing neoadjuvant treatment and cancer resections. Patients admitted between 2016 and 2022 were included in the study. Based on the histology of the tumor, patients were categorized into a lung adenocarcinoma group (LUAD) and squamous cell carcinoma group (SQCA). Ninety-five patients with non-small-cell lung cancer were included in the study. A total of 58 (61.1%) and 37 (38.9%) patients were included in the LUAD and SQCA groups, respectively. Additionally, 9 (9.5%), 56 (58.9%), and 30 (31.6%) patients were categorized with a tumor regression score of I, II, and III, respectively. In multivariable analyses, histology of the primary tumor (SQCA), lymph node size in the preoperative CT scan (>1.7 cm), and absolute tumor size reduction after neoadjuvant treatment (>2.6 cm) independently predict effectiveness of tumor regression (OR [95% confidence interval, p-value] of 6.88 [2.40-19.77, p < 0.0001], 3.13 [1.11-8.83, p = 0.0310], and 3.76 [1.20-11.81, p = 0.0233], respectively). Age > 70 years, extended resection > one lobe, and tumor recurrence or metastasis were identified as significant independent predictors of reduced overall survival. Assessment of tumor size before and after neoadjuvant treatment might help to identify high-risk patients with decreased survival and to improve patient management and care.

Characteristics and Outcomes of Fetal Cardiac Rhabdomyoma With or Without mTOR Inhibitors, a Systematic Review and Meta-Analysis.

To investigate the characteristics and outcomes of fetal cardiac rhabdomyoma with or without prenatal use of mammalian target of rapamycin inhibitor (mTORi). We systematically searched PubMed, Scopus, and Web of Science until June 2023. Studies reporting on pregnancies with fetal cardiac rhabdomyoma were included. A meta-analysis of proportions was conducted only on studies that included three or more cases. A systematic review included 61 studies reporting on 400 fetuses with cardiac rhabdomyoma, of which 52 studies (389 fetuses) had expectant management and 9 studies (11 fetuses) were managed with mTORi. The meta-analysis included 26 studies reporting on 354 fetuses. Prenatally, 14% (95% CI 4-36) had pericardial effusion, 13% (95% CI 6-27) had arrhythmia, 16% (95% CI 7-31) had outflow tract obstruction, and 10% (95% CI 4-21) had hydrops. Fetal demise occurred in 12% (95% CI 5-30). Before delivery, tumor size reduction was noted in 13%, and after birth in 58%. Following birth, 8% (95% CI 3-14) had neonatal death and 9% (95% 4-17) required cardiac surgery. 60% (95% CI 41-79) of cases were diagnosed with tuberous sclerosis. Seizures were reported only in cases with a tuberous sclerosis diagnosis (41/71 infants). For the 9 studies reporting all together on 11 fetuses with tuberous sclerosis receiving prenatal mTORi, they showed improvement in the size of cardiac rhabdomyoma as well as outflow obstruction and none had fetal demise or neonatal death, and none required postnatal cardiac surgery. We report on the natural history of prenatal cardiac rhabdomyoma, including characteristics, progression, and survival. We report 11 fetuses with tuberous sclerosis and cardiac rhabdomyoma receiving prenatal mTORi, showing promising results.

Improvement of an ionic wind blower’s flow characteristic by auxiliary electrodes for thermal management of light-emitting diodes

Electronic device advancements in power, size reduction, and integration have resulted in increased heat flux and operating temperatures, negatively impacting the reliability of electronics. Ionic wind cooling is a new, environmentally friendly, and energy-efficient thermal management approach that effectively cools high heat flux local heat sources. However, enhancing ionic wind strength continuously can be challenging. In this study, an ionic wind blower consisting of an emitting electrode, a collecting electrode, and auxiliary electrodes is constructed. The experimental verification confirms the supplemental acceleration capacity of the auxiliary electrodes. When determining the optimal operating voltage applied to the auxiliary electrodes, the power consumption of the system and the intensity of the output ionic wind are taken into consideration. Theblower’s operational and structural factors, such as the emitter structure, discharge gap, and distance between the auxiliary electrodes and collectors, are optimized according to how they affect the device’s functional qualities. The improved blower’s heat dissipation ability is evaluated by cooling an LED chip. The results demonstrate that the system performs optimally with an emitter having seven needles, a discharge gap of 5 mm, and 9 mm between the auxiliary electrodes and the collector. The wind speed reaches 2.47 m/s, while the power consumption is only 1.6 W. Compared to the absence of auxiliary electrodes (47.7 W/(m2∙K)), the system’s mean convective heat transfer coefficient can reach 61.12 W/(m2∙K), resulting in a temperature reduction of the LED chip by up to 41.6 °C. With increasing voltage, the heat transfer enhancement ratio improves, enabling a blower with auxiliary electrodes to provide significant cooling while consuming less power.

M2 Macrophage-Derived Exosomes Inhibit Atherosclerosis Progression by Regulating the Proliferation, Migration, and Phenotypic Transformation of Smooth Muscle Cells.

Vascular smooth muscle cell (VSMC) intimal migration, proliferation, and phenotypic transformation from a contractile to a synthetic state are hallmarks of the progression of atherosclerotic plaques. This study aims to explore the effects of exosomes derived from M2 macrophages (ExoM2) on the pathological changes of VSMCs in atherosclerosis (AS). Cell Counting Kit-8 (CCK8) and wound healing assays were used to examine the impact of ExoM2 on platelet-derived growth factor-BB (PDGF-BB)-induced VSMC proliferation and migration, respectively. Western blotting was employed to analyze changes in the expression levels of contractile markers (e.g., alpha-smooth muscle actin [α-SMA]) and synthetic ones (e.g., osteopontin [OPN]) in VSMCs with or without ExoM2 treatment. ApoE-⁣/- mice on a high fat diet were utilized to observe the effects of ExoM2 on plaque progression and stability. Serial histopathological analysis was performed to elucidate the cellular mechanisms underlying the atheroprotective effects of ExoM2. Compared with controls, ExoM2 significantly inhibited PDGF-BB-induced VSMC proliferation, migration, and phenotypic transformation in vitro. In ApoE-⁣/- mice, ExoM2 treatment led to a marked reduction in plaque size, necrotic core area, the CD68/α-SMA ratio, and matrix metalloproteinase 9 (MMP9) and OPN levels, while enhancing plaque stability. ExoM2 inhibit AS progression by regulating VSMC proliferation, migration, and phenotypic transformation.

Evaluation of water resource balance in the Urmia Lake Basin: Integrating carrying capacity and water footprint model for sustainable management

Given the scarcity of water resources and the ever-growing demand, effective water flow management becomes crucial. The Lake Urmia Basin, a critical region in Iran, faces severe water scarcity exacerbated by climatic changes, land use alterations, over-extraction of groundwater, and mismanagement. This study evaluates the water resource balance by integrating carrying capacity and water footprint models to assess sustainable water management practices. Utilizing the InVEST model, water provisioning ecosystem services have been estimated as the supply, while the ecological footprint method quantifies water demand across various sectors. Three scenarios are evaluated: continuation of the current situation, an ideal scenario with substantial reductions in water consumption and pollution, and implementation of operational programs. The findings reveal significant water deficits in certain sub-basins, highlighting the need for a fundamental revision of resource allocation based on each region’s ecological capacity. Lake Urmia, the central nucleus of the basin, has experienced a significant size reduction, approximately 10 % of its original size, from the early 1990s to 2018, due to climatic changes, land use alterations, over-extraction of groundwater, and mismanagement. The environmental deficit in some sub-basins raises serious concerns, even in the best scenario where ecological footprint reduction is considered. These results underscore the effectiveness of ecosystem-based comprehensive capacity analysis for sustainable watershed development. This research offers a unified spatial system for quantifying ecological footprints and land resilience, providing critical insights into addressing water scarcity and supporting holistic regional planning.

Impacts and effectiveness of sidewall treatment on the spatially resolved optical properties and efficiency enhancement for GaN-based blue and green micro-LEDs

InGaN-based micro-light-emitting diodes (micro-LEDs) are crucial for efficient full-color micro-displays, but suffer from severe degradation of external quantum efficiency (EQE) with size reduction. In this study, the impacts of chemical treatment on the sidewall condition and the EQE of the 10 μm InGaN-based blue and green micro-LEDs were demonstrated. The sidewall treatment can effectively alleviate the residual damage caused by dry etching and suppress the nonradiative recombination, facilitating the achievement of high internal quantum efficiency (IQE). Nevertheless, the sidewall light extraction of the micro-LEDs with smoother sidewall morphology after tetramethylammonium hydroxide (TMAH) treatment is lower than those with rough and prismatic sidewall surface. By utilizing microscopic hyperspectral imaging, the difference in chromatic properties between the inner region and the whole micro-LED caused by dry etching can hinder the stability of chromatic properties of InGaN-based blue micro-LEDs, which can be alleviated by sidewall treatment. The optimal duration for TMAH treatment is obtained as 10 min for both blue and green micro-LEDs, which is the consequence of the compromise between sidewall light extraction efficiency (LEE) and IQE, whereas the improvement of IQE by sidewall treatment for green micro-LEDs is less significant compared to that for blue ones, which can be attributed to the higher dislocation density and recombination via localized states of green InGaN micro-LEDs. This work demonstrates the evolution of IQE and LEE for blue and green micro-LEDs with different sidewall treatment strategies to achieving the ultimate EQE.

A novel subtype classification and corresponding surgical strategies for spinal dural cysts –a report of 104 cases

BACKGROUNDThe nomenclature, classification, and surgical approaches for spinal dural cysts (SDCs) remain a subject of controversy. PURPOSEThe present study proposes a novel subtype classification system and corresponding surgical strategies, with the aim of enhancing comprehension of this entity and standardizing surgical treatment. STUDY DESIGNA retrospective review. PATIENT SAMPLEA total of 104 patients with SDCs underwent novel subtype classification and corresponding surgical strategies from January 2015 to December 2021. Fifty-four patients who underwent conventional surgery from January 2012 to December 2014 as the control group for preliminary validation. OUTCOME MEASURESThe outcomes are categorized into 4 levels: excellent, good, unchanged, and deteriorated, based on neurological improvement and pain relief. Grades of excellent and good were identified as improvements. Follow-up magnetic resonance imaging and complications were also evaluated. METHODSBased on the shared pathogenic factor of dural defects, the dural-associated cysts in the spinal canal are uniformly referred to as SDCs. They are further classified into Type 1 (no nerve roots) and Type 2 (containing nerve roots), with 4 additional subtypes based on the shape of the leak and the flow of leakage. The fissure-shaped leak of Type 1a SDCs is directly sutured, whereas the aperture-shaped leak of Type 1b is repaired using a patch. Low-flow leakage of Type 2a is directly sealed using a combination of adipose tissue and fibrin glue, whereas high-flow Type 2b necessitates suturing at the end of the leak to attenuate cerebrospinal fluid flow prior to sealing. RESULTSThe follow-up period averaged 23.8 months. Excellent or good outcomes were achieved in 100%, 88.9%, 100%, and 97.3% for the 4 subtypes respectively. The overall improvement rate of SDCs was 97.1%, which was significantly higher than that of the conventional surgery group (85.2%, p=.008). MRI follow-up showed a significant reduction in cyst size of 100%, 100%, 97.8%, and 97.3% for the 4 subtypes, respectively. The primary complications included pseudomeningocele in 4 cases (3.8%) and delayed wound healing in 5 cases (4.8%). The complication rate was also significantly lower than that of the control group (8.7% vs 24.1%, p=.008). CONCLUSIONSSubtyping SDCs based on the variation of leaks and leakage can enable more targeted surgical strategies, which are helpful for improving treatment effectiveness and reducing complications.

Pyroxylin-based liquid bandage forms a mechanically active protective film to facilitate skin wound healing in mice

Wound healing requires interplay between cells and molecules. Recent evidence has demonstrated that liquid bandages promote wound healing by forming a protective barrier against contamination, attenuating inflammation, and enhancing re-epithelialization and angiogenesis. In this study, we evaluated the wound healing activity of pyroxylin-based liquid bandage (LiQuiPlast®) in eight-week-old C57BL/6 male mice by generating a single 4 mm diameter full-thickness excisional skin wound on the dorsum. In the LiQuiPlast® group, the liquid bandage was applied on day 0 and was replaced every four days. Wound size was monitored every day for two weeks. The results showed that LiQuiPlast® was mechanically active (induced wound contraction), which promoted a significant wound size reduction (27 %−39 %, compared to the control group) on days 1–4 postinjury. In addition, a significant reduction in wound size was observed again in the LiQuiPlast® group (25 %−29 %, compared to the controls) on days 8−9 postinjury. LiQuiPlast®-treated wounds showed no scab. Immunohistochemistry analyses displayed a reduction in neutrophils and tumor necrosis factor-α levels in LiQuiPlast®-treated wounds, compared to the control group on day 4 postinjury (the inflammatory phase). In addition, LiQuiPlast®-treated mice had enhanced keratinocyte proliferation than control mice during this time. On day 13 postinjury, LiQuiPlast® significantly reduced hypertrophic scarring and enhanced expression and reorganization of collagen fiber compared to control mice. In conclusion, we show that LiQuiPlast® acts as a mechanically active protective film, which promotes moist wound healing by promoting wound contraction, no scab formation, attenuated inflammation, enhanced keratinocyte proliferation, and decreased scarring.

Effects of scanning strategy and scanning speed on microstructures and mechanical properties of NiTi alloys by laser powder bed fusion

In this work, the influence of processing parameter including laser scanning strategy and scanning speed on grain morphology and crystalline orientation of NiTi alloys fabricated by laser powder bed fusion (L-PBF) was comprehensively investigated from the perspective of crystal growth. The results show that as the interlayer rotation angle increases from 0 to 90°, the grain morphology changes from the coarse columnar to columnar-and-equiaxed shapes, accompanied by the texture intensity first rising then reducing. Besides, increasing the scanning speed leads to a transition in grain morphology from columnar to equiaxed crystals, as well as a reduction in grain size and texture intensity. The recovery mechanism caused by different thermal histories under various scanning speeds was discussed, with a particular focus on dislocation density and its distribution. The results demonstrate that the dislocation density gradually increases with the increase of scanning speed, leaving behind more dislocations within the matrix. Furthermore, the effects of scanning speed and building direction on the compressive behavior were investigated through identifying the difference of microstructure in the NiTi alloys by L-PBF. This study highlights the relationship among processing, microstructure, and mechanical performance, paving the way for tailoring and designing microstructures and mechanical properties for the NiTi alloys by L-PBF.

Microstructure and Microhardness Evolution of Mg-8Al-1Zn Magnesium Alloy Processed by Differential Speed Rolling at Elevated Temperatures.

Mg-8Al-1Zn magnesium alloy was successfully processed using deferential speed rolling (DSR) at temperatures of 400 and 450 °C for thickness reduction of 30, 50, and 70% with no significant grain growth and dynamic recrystallization. Using optical microscopy (OM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM), the rolled microstructures were examined. Although the results indicate a slight reduction in grain size from the initial condition, the DSR processing of alloy at an elevated temperature was associated with a significant number of twins and a distribution of the fine particles of the second phase. The strength in terms of microhardness measurements and strain hardening in terms of shear punch testing was significantly improved in the rolled microstructure at room temperature. The existence of twins and widely distributed second-phase fine particles at twin boundaries reflected positively on the extent of the elongations in terms of shear displacements when microstructures were tested at elevated temperatures in the shear punch testing.

Fracturing, comminution and grain-size-sensitive creep as a record of coseismic loading in the middle-crust: Insights from the Urtiga mylonitic pluton (NE Brazil)

Evidence for mid-crustal seismic slip is scarce due to the limited capacity of downward propagation of rupture damage imposed by the confining pressure. Nevertheless, structures indicative of elevated stresses occur throughout the crustal profile. To further investigate this setting, we have studied the fabrics of the Urtiga pluton, emplaced at the south Patos shear zone (northeast Brazil), a major Neoproterozoic crustal boundary. K-feldspar and plagioclase porphyroclasts are fractured, with fine-grained K-feldspar + plagioclase mixtures filling cracks. The edges of the clasts are rimmed by fine feldspar grains that form a fine-grained matrix. Quartz ribbons are parallel to the mylonitic foliation and show microstructures and a fabric indicating deformation via dislocation creep. Chemical compositions of feldspars are typically similar between porphyroclasts, fractures and matrix, with plagioclase grains locally being more albitic within fractures. Crystallographic preferred orientations (CPO) in grains within fractures are host-controlled by the adjacent porphyroclast, while fine grains rimming the clasts show a weak CPO and are mostly strain-free. These characteristics suggest that grain size reduction in the Urtiga mylonitic pluton occurred through fracturing and subsequent grain-size-sensitive creep under mid-crustal conditions, which were possibly attained via downward propagation of seismic rupture from the overlying seismogenic zone during transient, seismic slip episodes, giving rise to spatially related pseudotachylytes at the boundaries of the southern Patos shear zone.

Impact of photoaging on the chemical and cytotoxic properties of nanoscale zeolitic imidazolate framework-8

This study investigated the influence of photoaging on a nanoscale metal-organic framework (MOF), truncated rhombic dodecahedron nano-zeolitic imidazolate framework-8 (nZIF-8), focusing on its oxidative stress, inflammation, and implications for pulmonary diseases. We observed significant photodegradation-induced transformations in nZIF-8, characterized by a reduction in particle size from 200.5 to 101.4 nm and notable structural disintegration after prolonged exposure to simulated solar radiation. This alteration resulted in a marked decrease in oxidative cytotoxicity in BEAS-2B cells, which was attributed to changes in surface properties and reduced reactive oxygen species (ROS) production. Gene expression analysis further revealed a decrease in cytotoxic and inflammatory responses, which potentially lowers the risk of chronic obstructive pulmonary disease (COPD). Aged nZIF-8 also showed diminished capacity to induce pro-inflammatory cytokines and influence COPD-related gene expression, reducing its potential to exacerbate COPD pathogenesis. Our findings highlight the critical need for comprehensive safety evaluations of these materials, while considering their long-term environmental and biological impacts. The diminished cytotoxicity and inflammatory potential of aged nZIF-8 highlighted its enhanced suitability for broader applications, indicating that photoaging may lead to safer and more sustainable material utilization.

Investigation of the thermal-hydraulic characteristics of SCO2 in a modified hybrid airfoil channel

The performance of printed circuit heat exchangers (PCHE) is critical to ensuring the efficiency and compactness of supercritical carbon dioxide (SCO2) Brayton cycle systems used in advanced nuclear energy. In order to improve the performance of the airfoil fin channel PCHE, the effect of the airfoil fin size on the channel performance is investigated and the effect of the airfoil fin arrangement parameters on the channel performance is analyzed by the Taguchi method. Particularly, a modified hybrid airfoil fin channel adapted to the changes in thermophysical properties of SCO2 is proposed. The results show that the heat transfer characteristics of the PCHE channel are enhanced and the flow characteristics are weakened with the reduction of the airfoil fin size, and the comprehensive performance firstly increases and then decreases. Taking the comprehensive performance of the channel as the optimization objective, the best combination of airfoil fin arrangement parameters is 2 mm for transverse spacing, 10 mm for longitudinal spacing, and 4 mm for staggered spacing. Compared with the traditional airfoil finned channel, the modified hybrid airfoil fin channel has significantly improved the comprehensive performance by 2.8%–13.2 %.

Improved lightweight infrared road target detection method based on YOLOv8

Infrared-based road scene object detection algorithms often face issues with excessive parameters and computational demands, making them incompatible with edge devices having constrained computational capabilities. This paper introduces an enhanced lightweight infrared-based road object detection algorithm based on YOLOv8n. Firstly, a streamlined network architecture is devised by merging YOLOv8n’s C2f module with PConv, creating a lighter module and reducing the neural network’s downsampling rate of infrared images. This strategy reduces redundant computations and memory access, preventing the loss of fine details in infrared images caused by deep convolutional neural networks. Additionally, the model’s accuracy in detecting infrared targets is significantly enhanced through the integration of the coordinate attention mechanism. Finally, replacing CIoU with Wise-IoU for bounding box regression in YOLOv8n accelerates the model’s convergence. Empirical findings indicate that in contrast to the YOLOv8n algorithm, the optimized model showcases a 34.17 % reduction in model size, a 40.35 % decrease in parameters, and a 4.8 % increase in average detection accuracy. This enhanced algorithm not only achieves a lightweight profile but also delivers superior performance on embedded edge devices.

Development of emulsion-filled calcium alginate gels for thermoresponsive release of flavor in plant-based meat analogs

We developed emulsion-filled calcium alginate gels (EF-CAG) as a novel thermoresponsive carrier for plant-based meat analogs (PBMA), designed to mimic the cooking-induced flavor release of real meat. Optimized to maximize flavor release upon heating, EF-CAG demonstrated a thermoresponsive release of 51% with a notable size reduction. Initial release profiles varied between media; a burst release of 43% occurred in water within the first 5 min, while <1% was released in air. EF-CAG consistently increased flavor release in PBMA during cooking, without adversely affecting appearance or flavor stability, maintaining flavor retention at 4 °C for 10 days. The sensory evaluation confirmed that EF-CAG successfully masked the beef flavor before cooking and enhanced its release afterward. Our findings suggest that EF-CAG can be effectively used as a flavoring agent for PBMA, offering similar flavor attributes to real meat during cooking.

Explaining the correlation between subharmonic amplitude and ambient pressure for subharmonic-aided pressure estimation (SHAPE)

BackgroundSubharmonic aided pressure estimation (SHAPE) is an innovative non-invasive technique that leverages ultrasound subharmonic imaging to estimate pressure. This method exploits the “negative correlation between subharmonic amplitude and ambient pressure” observed in experimental settings. Despite extensive experimental validation and some promising results in clinical studies, the underlying mechanism of SHAPE remains incompletely understood. Although some studies have attempted to provide theoretical explanations, definitive conclusions have yet to be reached. In addition, theoretical investigations have mainly focused on the steady oscillation of bubbles under long pulse excitation, which contrasts with the short pulse excitation required for clinical SHAPE applications. An understanding of the SHAPE principle under short pulse excitation is needed. MethodsThe exponential elasticity model (EEM) was used to simulate Sonazoid bubbles, and a probe-to-probe acoustic propagation model was introduced to mimic a practical SHAPE scenario. The simulated acoustic signals in response to three-cycle sinusoidal pulse excitations were analyzed for spectral composition. The relationship between microbubble oscillation patterns and subharmonic characteristics was identified through detailed investigation. ResultsFor the excitation pulse of 2.5 MHz frequency and 350 kPa magnitude, bubbles larger than the resonance radius (2.29 μm) exhibited significant subharmonics in the magnitude spectrum, while bubbles smaller than the subharmonic resonance radius (3.85 μm) showed the activity of scattering subharmonic energy and the sensitivity to ambient pressure. The emergence of subharmonics when increasing excitation power was related to the increasing amplification of the bubble self-oscillation and the period-doubling features in the acoustically forced oscillation. The negative correlation between subharmonic amplitude and ambient pressure was attributed to the reduced self-oscillation caused by increasing ambient pressure and hence bubble size reduction. Microbubbles falling between 2 and 3 μm showed the desired subharmonic sensitivity to ambient pressure under the specified excitation conditions. ConclusionThe transient oscillatory behavior of microbubbles in response to short pulse excitation, characterized by a ringing down self-oscillation after the acoustic forcing effect has ceased, is crucial for understanding the subharmonic emergence and the observed negative correlation between subharmonic amplitude and ambient pressure. The proposed concepts of subharmonic resonance radius, subharmonic-significant bubbles, and subharmonic-active bubbles provide valuable insights into the diverse subharmonic behavior of microbubbles. The theoretical explanation of this negative correlation highlights the importance of using subharmonic-significant-and-active bubbles for SHAPE applications.

Optimization and clinical evaluation of hyaluronic acid proniosome loaded in-situ gel for the management of recurrent aphthous stomatitis

Recurrent aphthous stomatitis (RAS) is a frequent unpleasant oral condition that causes ulcers. Hyaluronic acid (HY) is an inherent polymer owing antioxidant, anti-inflammatory, and anti-bacterial patterns. D-optimal response surface design was used to investigate the impact of surfactant type (Span 80 or Brij®35) and the concentration of HY (0.5 % or 1), on three selected dependent variables: zeta potential (ζ, ZP, R1), particle size (PS, R2), and entrapment efficiency (%EE, R3). The optimized HYPN was then loaded in thermosensitive in-situ gel. The clinical efficacy in patients with RAS was evaluated by measuring the improvement in pain levels (using the Visual Analog Scale), reduction in ulcer size, enhancement in quality of life (measured by OHIP-14 scores), and analysis of pro-inflammatory Tumor Necrosis Factor Alpha (TNF-α) and total antioxidant capacity (TAC) in saliva samples. The optimized formula obtained a mucoadhesive strength of 26 ± 1.76 N/m2. A burst release of 38 ± 1.56 % after 2 h was obtained followed by 80 ± 2.21 % till 24 h, with a transbuccal flux (Jss) 425 ± 7.9 μg/h.cm2. Clinically, a significant difference (P ≤ 0.001) in pain scores, ulcer diameter, OHIP-14 scores, TAC, and TNF-α between the in-situ gel containing HYPN and the placebo gel was found. The mean values for the number of recurrences at 6 months follow-up were 2.55 ± 1.13 and 4.63 ± 1.75, respectively, before and after treatment. The thermosensitive mucoadhesive in-situ gel seems to be a promising therapeutic choice for RAS treatment.