Site Usage Research Articles

The Impact of Social Networking Sites on the Youth with Special Reference to Chennai City

Objective: This study aimed to know the perception of individuals towards mobile banking and find out the satisfaction levels of users towards mobile banking.Methods: A prospective study was conducted among customers in Chennai, India. A total number of 155 participants. (Male 88, Female 66 and Prefer not to say 1). Data were processed and analyzed by the Statistical Package for social science. Descriptively analysis and chi squire was performed. P values < 0.05 were considered statistically significantResults: A total of 155 respondents of 56.77 percent of respondents are male, and 42.58 percent of respondents are female and prefer not to say 0.65 percent in the usage of Social Network Sites. It is found that the majority of respondents are Male. The majority of the respondents are the purpose of using social networking sites in (SSN) 24.52% just for fun and entertainment (SNS). Here the majority of the respondents are preferred social networking sites are Whatsapp.Conclusion: After getting all findings and discussed the end of collected data, the researcher recommends some measures to use of social media in the right direction and utilize social media favorable and appropriate manner to its users. Positive use of social media can develop the youth’s skill, better living style, Career, adopt new trends, and anthropology so on. The study is useful to the teacher, parents, educators in particular, and society in general to understand the behavior of the youth of today.

Highlights from Recent Cancer Literature

Highlights from Recent Cancer Literature

Factors Influencing Intention to Use Social Media Sites for Holiday Destination Selection by the Young Generation

The main objective of the study was to examine the factors that influence attitude towards usage of social media for holiday destination selection by the young generation, specifically university students and to explore the antecedents of behavioural intention to use social media sites. A structured questionnaire was used to gather data for this study, yielding a total of 350 responses suitable for analysis. Regression analysis was employed to test the hypotheses. The results revealed that social presence, perceived ease of use, perceived usefulness and perceived enjoyment all positively influence attitude towards use of social media sites. Revealed by the results also is that perceived behavioural control, subjective norm and attitude also positively influence behavioural intention. Social media developers should develop sites, which are attractive, fun and exciting so that the young generation can be attracted. Moreover, destination marketers must ensure that all the important information is posted on their social media sites pages. Studies have been conducted on the usage of social media sites for searching travel related products, but limited research has been done focusing specifically on the usage of these sites by the young generation, such as university students in the South African context.

Forecasting the Impact of Social Media Advertising among College Students using Higher Order Statistical Functions

Nowadays, social media has emerged as one of the activity among the users on their day-to-day life activity. This research work plans to develop a statistical review that concerns on social media advertising among college students from diverse universities. The review analysis on social media advertising is given under six sections such as (i) Personal Profile; (ii) Usage; (iii) Assessment; (iv) Higher Order statistics like Community, Connectedness, Openness, Dependence and Participation; (v) Trustworthiness such as Trust, Perceived value and Perceived risk; and (vi) Towards advertisement which involves attitude towards advertisement, response towards advertisement and purchase intension. The initial stage is on questionnaire preparation based on social media networking. The records stated in the questionnaire are intimately taken about the usage of social media sites and the advertisement on social media networking. In the second stage, the planned questionnaire is distributed over college students from diverse universities. The entire questions are made mandatory in this questionnaire and subsequent to this, the students from various universities are demanded to fill up their response to this questionnaire. These responses from the students are then taken for analysis purpose. In this research work, the analysis is performed on the basis of higher-order statistical analysis that favorably concerned on correlation coefficients and entropy. This in turns helps to determine the correlation and entropy among the response towards the social media network.

Linkage between social identity creation and social networking site usage: the moderating role of usage intensity

PurposeInteraction and communication through social networking sites (SNSs) has witnessed exponential growth every year. The rising popularity of this platform has made researchers take a deeper look at this phenomenon and try and study it in a structured fashion. The purpose of this study is to investigate the moderating role of SNS usage intensity on the relationship between social capital and social identity. There are very few published literature studies available and none in the context of a developing economy, which is undergoing enormous digital transformation. While studies like this have been done in abundance in the Western world, it is still a new approach in this part of the world. Even though the variables that are being studied have been adapted from the work done earlier by other researchers, the application and interpretation are very different, primarily because of the context.Design/methodology/approachA primary online survey was conducted to collect data for this study. A majority of 258 respondents were in the age group of 20–40 years. Most of them had an undergraduate and/or a postgraduate degree and spent an average of 70% of their weekly time on social media. The sample size was balanced in terms of gender (male/female) as well. To validate the research model and test the hypotheses of the study, through two analysis phases including measurement model and structural model, reliability analysis, confirmatory factor analysis (CFA), correlations and hierarchical multiple regression were deployed. The CFA was applied to assess the validity of the four factors under study.FindingsFactors that were studied in this article were checked for content validity and reliability. Cronbach's alpha values were <1.0 indicating the reliability of the factors taken for the study. Hierarchical multiple regression showed that with the increase in bridging and SNS usage intensity, social identity also increases at a high level of bridging. Similar results were observed when regression was conducted for bonding and SNS usage intensity. Thus, the hierarchical multiple regression analysis showed that SNS usage intensity positively moderated the effects of social capital on social identity. Hence, the two hypotheses were supported.Originality/valueThe results of this study are significant for business organizations and society as well. A similar type of study in the context of an economy, which has embarked on the path of digitization as a state-sponsored policy has not been reported.

Accuracy of road construction preliminary estimate: examining the influencing factors

PurposePreparation of preliminary estimate is difficult owing to the lack of full project details in the early phases of the construction project. This paper seeks to assess the estimation techniques used for road projects and the critical factors affecting their accuracy in the Ghanaian construction industry.Design/methodology/approachQuantitative research design was adopted and questionnaire was designed to retrieve data. The target population were engineers and quantity surveyors who were contacted using an e-questionnaire through their professional bodies owing to location constraints. Retrieved data were analysed using descriptive and exploratory factor analysis. In order to compare the opinions of the respondents, the Mann–Whitney U-test was employed.FindingsThe survey revealed that subjective, parametric, comparative and analytical estimations are in use in Ghana. The most critical factors influencing the accuracy of estimation techniques are improper project planning, insufficient preliminary site investigation and usage of shortcuts, among others.Research limitations/implicationsThis study was limited to Accra, Ghana, due to time and distance constraint.Practical implicationsFor accuracy of preliminary estimates to be improved, estimators being the custodian of the estimate are expected to be devoid of errors such as arithmetic calculation errors, inaccurate quantity measurement and error of omission. The usage of estimating software can eliminate these human errors.Originality/valueThe study will assist policymakers and stakeholders in aligning mitigative actions for factors influencing preliminary estimate of road projects with defined clusters rather than basic ranks. With attention focussed on the characteristics of each cluster, accuracy of preliminary estimate can be improved.

Surgical patients’ use of, and attitudes towards, the internet for e-patient activities in Germany and Oman

IntroductionE-patient activities are known to impact upon the patient-doctor relationship and on surgical outcomes. In Oman, there is no published information about the e-patient. The aim of this study, conducted at two surgical sites, was to investigate surgical e-patients’ use of, and attitudes towards, the Internet, and the possible impact on the delivery of healthcare. Materials and methodsA convenience sample of 83 German and 93 Omani patients at the two surgical sites were given an in-house paper-based questionnaire, based on e-patient activities described in the literature. Descriptive statistics like means, standard deviations and frequencies were calculated. ResultsThere were many similarities in usage and attitudes. Omani patients showed much greater knowledge and usage of sites and apps, used the Internet more for health-related activities (26.9% vs. 12.0%), and had a greater proportion of their physician encounters through email (10.0% vs. 4.0%) and social media (15.2% vs. 1.8%). More Omani patients brought information from the Internet than German patients (13.5% vs. 6.0%). Patients from both countries were generally positive about bringing material from the Internet to the consultation. Discussion and conclusionBoth sites indicated typical e-patient activity and attitudes as described in the literature. Age and type of condition (chronic vs. acute) may explain the differences to some extent, but this was not consistent. Socio-cultural differences between the two countries may have a great influence on the usage.

White Matter Microstructural Properties Associated with Impaired Attention in Chronic Schizophrenia: A Multi-Center Study

Attention as a key cognitive function is impaired in schizophrenia, interfering with the normal daily life of the patients. Previous studies on the microstructural correlates of attention in schizophrenia were limited to single fibers, did not include a control group, or did not adjust for drug dosage. In the current study, we investigated the association between microstructural properties of the white matter fibers and attention tests in 81 patients and 79 healthy controls from the Mind Clinical Imaging Consortium database. Integrity measures of superior longitudinal fasciculus, cingulum, genu, and splenium were extracted after tractography. Using an interaction model between diagnosis and microstructural properties, and adjusting for age, gender, acquisition site, education, and cumulative drug usage dose, and after correcting for family-wise error, we showed decreased integrity in the patients and a significant negative association between fractional anisotropy of the tracts and trail making test part A with a greater expected decrease in the attention per unit of decrease of integrity in the patients compared to the healthy controls. Our findings suggest that decreased integrity of the bilateral cingulum, and splenium, are independent of the cumulative drug dosage, age, gender, and site, and may underlie the impaired attention in the schizophrenia.

Global transcriptome analysis reveals circadian control of splicing events in Arabidopsis thaliana.

The circadian clock of Arabidopsis thaliana controls many physiological and molecular processes, allowing plants to anticipate daily changes in their environment. However, developing a detailed understanding of how oscillations in mRNA levels are connected to oscillations in co/post-transcriptional processes, such as splicing, has remained a challenge. Here we applied a combined approach using deep transcriptome sequencing and bioinformatics tools to identify novel circadian-regulated genes and splicing events. Using a stringent approach, we identified 300 intron retention, eight exon skipping, 79 alternative 3' splice site usage, 48 alternative 5' splice site usage, and 350 multiple (more than one event type) annotated events under circadian regulation. We also found seven and 721 novel alternative exonic and intronic events. Depletion of the circadian-regulated splicing factor AtSPF30 homologue resulted in the disruption of a subset of clock-controlled splicing events. Altogether, our global circadian RNA-seq coupled with an in silico, event-centred, splicing analysis tool offers a new approach for studying the interplay between the circadian clock and the splicing machinery at a global scale. The identification of many circadian-regulated splicing events broadens our current understanding of the level of control that the circadian clock has over this co/post-transcriptional regulatory layer.

Determination of RNA structural diversity and its role in HIV-1 RNA splicing.

Human immunodeficiency virus-1 (HIV-1) is a retrovirus with a 10-kb single-stranded RNA genome. HIV-1 must express all of its gene products from the same primary transcript, which undergoes alternative splicing to produce diverse protein products, including structural proteins and regulatory factors1,2. Despite the critical role of alternative splicing, the mechanisms driving splice-site choice are poorly understood. Synonymous RNA mutations that lead to severe defects in splicing and viral replication indicate the presence of unknown cis-regulatory elements3. We use DMS-MaPseq to probe the structure of HIV-1 RNA in cells and develop an algorithm called Detection of RNA folding Ensembles using Expectation-Maximization (DREEM), which reveals alternative conformations assumed by the same RNA sequence. Contrary to previous models, which analyzed population averages4, our results reveal the widespread heterogeneous nature of HIV-1 RNA structure. In addition to confirming that in vitro characterized alternative structures for the HIV-1 Rev Responsive Element (RRE) exist in cells, we discover alternative conformations at critical splice sites that influence the ratio of transcript isoforms. Our simultaneous measurement of splicing and intracellular RNA structure provides evidence for the long-standing hypothesis5–7 that RNA conformation heterogeneity regulates splice site usage and viral gene expression.

Undergraduate biochemistry student difficulties with topics requiring mathematical skills: use of an online maths skills support site

ABSTRACT A number of areas of difficulty in basic numeracy and mathematical skills were identified, which hinder students’ success in a foundational biochemistry course. Many students have difficulty with the concepts of pH and buffers (requiring proficiency with logarithms) and enzyme kinetics (requiring algebraic manipulations and graphing). The voluntary use by students of an ‘in house’ online support site, the Maths Skills Site, to address these difficulties was varied and seemed to be motivated by assessment deadlines, as well as deliberate instructions to refer to the relevant Maths Skills Site modules when completing assessment. The majority of the users of the online support site were those who performed poorly on a diagnostic test administered at the beginning of the course. There was a small but significant effect of Maths Support Site usage with users achieving improved grades for graphing functions compared to non-users. The combination of mathematical backgrounds and prior academic performance also correlated with success in first-year university biochemistry.

Regulation of alternative polyadenylation in the yeast Saccharomyces cerevisiae by histone H3K4 and H3K36 methyltransferases.

Adjusting DNA structure via epigenetic modifications, and altering polyadenylation (pA) sites at which precursor mRNA is cleaved and polyadenylated, allows cells to quickly respond to environmental stress. Since polyadenylation occurs co-transcriptionally, and specific patterns of nucleosome positioning and chromatin modifications correlate with pA site usage, epigenetic factors potentially affect alternative polyadenylation (APA). We report that the histone H3K4 methyltransferase Set1, and the histone H3K36 methyltransferase Set2, control choice of pA site in Saccharomyces cerevisiae, a powerful model for studying evolutionarily conserved eukaryotic processes. Deletion of SET1 or SET2 causes an increase in serine-2 phosphorylation within the C-terminal domain of RNA polymerase II (RNAP II) and in the recruitment of the cleavage/polyadenylation complex, both of which could cause the observed switch in pA site usage. Chemical inhibition of TOR signaling, which causes nutritional stress, results in Set1- and Set2-dependent APA. In addition, Set1 and Set2 decrease efficiency of using single pA sites, and control nucleosome occupancy around pA sites. Overall, our study suggests that the methyltransferases Set1 and Set2 regulate APA induced by nutritional stress, affect the RNAP II C-terminal domain phosphorylation at Ser2, and control recruitment of the 3′ end processing machinery to the vicinity of pA sites.

DNA Methylation Regulates Alternative Polyadenylation via CTCF and the Cohesin Complex

Dysregulation of DNA methylation and mRNA alternative cleavage and polyadenylation (APA) are both prevalent in cancer and have been studied as independent processes. We discovered a DNA methylation-regulated APA mechanism when we compared genome-wide DNA methylation and polyadenylation site usage between DNA methylation-competent and DNA methylation-deficient cells. Here, we show that removal of DNA methylation enables CTCF binding and recruitment of the cohesin complex, which, in turn, form chromatin loops that promote proximal polyadenylation site usage. In this DNA demethylated context, either deletion of the CTCF binding site or depletion of RAD21 cohesin complex protein can recover distal polyadenylation site usage. Using data from The Cancer Genome Atlas, we authenticated the relationship between DNA methylation and mRNA polyadenylation isoform expression invivo. This DNA methylation-regulated APA mechanism demonstrates how aberrant DNA methylation impacts transcriptome diversity and highlights the potential sequelae of global DNA methylation inhibition as a cancer treatment.

Roosting Site Usage, Gregarious Roosting and Behavioral Interactions During Roost-assembly of Two Lycaenidae Butterflies.

Lycaenidae is one of the larger of the world's butterfly families, based on number and diversity of species, but knowledge of roosting in this group is sparse. Zizina otis riukuensis and Zizeeria maha okinawana are two small lycaenids that are commonly found in urban settings and widely distributed across much of Asia. We conducted experiments on a university campus to determine the plant species and plant structures commonly used by these two blues when roosting. We also tested the hypothesis that gregarious roosting exists in these two blues by demonstrating the non-random distribution of roosting blues and the tight mapping of their roosts to the spatial distribution of specific plant species and/or specific plant structures, as well as by demonstrating behavioral interactions among individuals during roosting-assembly. We found that both Z. otis and Z. maha roosted primarily on flowers and fruits of Tridax procumbens and Vernonia cinerea. We also found that these blues formed conspicuous roosting aggregations with significant positive associations between the flowers and fruits of both T. procumbens and V. cinerea and the blues. Moreover, our behavioral observations showed that these blues expressed various levels of interaction during roosting gatherings. Based on these findings, we conclude that gregarious roosting exists in both Z. otis and Z. maha. To our knowledge, this paper represents one of the first demonstration of nocturnal gregarious roosting in lycaenids. This study also highlights the importance of institutional estates in providing roosting resources for butterflies in urban ecosystems.

Social Networking Site Usage and Its Impact on Depressive Symptoms among Older Men and Women in South Korea.

Background: We examined the rate of social networking site (SNS) usage and the relationship between SNS usage and depressive symptoms among older men and women in South Korea. Methods: The data were obtained from the Living Profiles of Older People Survey (LPOPS), which comprises a nationally representative sample of non-institutionalized Korean older adults living in the community. A total of 10,073 older persons (4286 men and 5787 women) were included in the analysis. Results: A total of 26.0% of the total study population reported to use social network sites. Of the men and women, 32.6 and 21.1%, respectively, used SNS. A higher educational level and higher equivalent household income, lower number of chronic illnesses, no limitation in daily activities, living with others, and having good relationships with friends and neighbors were associated with higher SNS usage among older men and women. SNS usage was significantly associated with reduced depression scores in older men, but not in women. Conclusions: The difference in SNS usage between older men and women might be partially explained by differential exposure to digital technologies and disadvantages in education and economic opportunities. Education programs for digitalization and opportunities to engage with technology need to be provided.

Pan‐tissue analysis of allelic alternative polyadenylation suggests widespread functional regulation

Alternative polyadenylation (APA) is a major layer of gene regulation. However, it has recently been argued that most APA represents molecular noise. To clarify their functional relevance and evolution, we quantified allele‐specific APA patterns in multiple tissues from an F1 hybrid mouse. We found a clearly negative correlation between gene expression and APA diversity for the 2,866 genes (24.9%) with a dominant polyadenylation site (PAS) usage above or equal to 90%, suggesting that their other PASs represent molecular errors. Among the remaining genes with multiple PASs, 3,971 genes (34.5%) express two or more isoforms with potentially functional importance. Interestingly, the genes with potentially functional minor PASs specific to neuronal tissues often express two APA isoforms with distinct subcellular localizations. Furthermore, our analysis of cis‐APA divergence shows its pattern across tissues is distinct from that of gene expression. Finally, we demonstrate that the relative usage of alternative PASs is not only affected by their cis‐regulatory elements, but also by potential coupling between transcriptional and APA regulation as well as competition kinetics between alternative sites.

Insights into the Involvement of Spliceosomal Mutations in Myelodysplastic Disorders from Analysis of SACY-1/DDX41 in Caenorhabditis elegans.

Mutations affecting spliceosomal proteins are frequently found in hematological malignancies, including myelodysplastic syndromes and acute myeloid leukemia (AML). DDX41/Abstrakt is a metazoan-specific spliceosomal DEAD-box RNA helicase that is recurrently mutated in inherited myelodysplastic syndromes and in relapsing cases of AML. The genetic properties and genomic impacts of disease-causing missense mutations in DDX41 and other spliceosomal proteins have been uncertain. Here, we conduct a comprehensive analysis of the Caenorhabditis elegans DDX41 ortholog, SACY-1 Biochemical analyses defined SACY-1 as a component of the C. elegans spliceosome, and genetic analyses revealed synthetic lethal interactions with spliceosomal components. We used the auxin-inducible degradation system to analyze the consequence of SACY-1 depletion on the transcriptome using RNA sequencing. SACY-1 depletion impacts the transcriptome through splicing-dependent and splicing-independent mechanisms. Altered 3' splice site usage represents the predominant splicing defect observed upon SACY-1 depletion, consistent with a role for SACY-1 in the second step of splicing. Missplicing events appear more prevalent in the soma than the germline, suggesting that surveillance mechanisms protect the germline from aberrant splicing. The transcriptome changes observed after SACY-1 depletion suggest that disruption of the spliceosome induces a stress response, which could contribute to the cellular phenotypes conferred by sacy-1 mutant alleles. Multiple sacy-1/ddx41 missense mutations, including the R525H human oncogenic variant, confer antimorphic activity, suggesting that their incorporation into the spliceosome is detrimental. Antagonistic variants that perturb the function of the spliceosome may be relevant to the disease-causing mutations, including DDX41, affecting highly conserved components of the spliceosome in humans.

Developmental regulation of cell type-specific transcription by novel promoter-proximal sequence elements.

Cell type-specific transcriptional programs that drive differentiation of specialized cell types are key players in development and tissue regeneration. One of the most dramatic changes in the transcription program in Drosophila occurs with the transition from proliferating spermatogonia to differentiating spermatocytes, with >3000 genes either newly expressed or expressed from new alternative promoters in spermatocytes. Here we show that opening of these promoters from their closed state in precursor cells requires function of the spermatocyte-specific tMAC complex, localized at the promoters. The spermatocyte-specific promoters lack the previously identified canonical core promoter elements except for the Inr. Instead, these promoters are enriched for the binding site for the TALE-class homeodomain transcription factors Achi/Vis and for a motif originally identified under tMAC ChIP-seq peaks. The tMAC motif resembles part of the previously identified 14-bp β2UE1 element critical for spermatocyte-specific expression. Analysis of downstream sequences relative to transcription start site usage suggested that ACA and CNAAATT motifs at specific positions can help promote efficient transcription initiation. Our results reveal how promoter-proximal sequence elements that recruit and are acted upon by cell type-specific chromatin binding complexes help establish a robust, cell type-specific transcription program for terminal differentiation.

Comparative genomic analysis of the 3' UTR of human MDM2 identifies multiple transposable elements, an RLP24 pseudogene and a cluster of novel repeat sequences that arose during primate evolution.

The MDM2 oncogene is a negative regulator of the p53 tumour suppressor. This relationship appears to have originated over a billion years ago. The human MDM2 gene encodes a variety of mRNAs with exceptionally long 3'UTRs (up to 5.7kb); however, it was unclear whether MDM2 3'UTRs from other species are similarly long or conserved at the sequence level. Here, we report that all but one of the primate species most closely related to humans (greater and lesser apes) have similarly long 3'UTRs with high sequence similarity across their entire length. More distantly related species (Old world monkeys and new world monkeys) tend to have shorter MDM2 3'UTRs homologous to the corresponding position of the human MDM2 3'UTR while non-primate species exhibit little similarity at all. Remarkably, DNA sequences downstream of the shorter primate 3'UTRs are syntenic with distal regions in the human and other ape MDM2 3'UTRs. These homologous non-transcribed intergenic and transcribed 3'UTR-encoding regions are comprised of a variety of transposable elements, an RLP24 pseudogene and a cluster of novel repeat sequences suggestive of another unknown transposable element. Our analysis suggests that the primary difference between long and short MDM2 3'UTRs is a switch in polyA site usage to include conserved transposable elements that remain intergenic in more distantly related primates. It will be important to determine the relative contribution of these elements to post-transcriptional and translational regulation of MDM2 and hence p53-mediated tumour suppression.

Unveiling knowledge quality, researcher satisfaction, learning, and loyalty

PurposeExamines how perceived knowledge quality influences researchers' satisfaction with academic social media (ASM) site use, perceived learning from use, and loyalty toward the site.Design/methodology/approachBuilt upon the theoretical grounding of the information system success framework, it was hypothesized that satisfaction, perceived learning, and loyal behavior toward an ASM site are all functions of the perceived quality of knowledge obtained. Data were collected by online survey from 348 researchers registered on ResearchGate and subjected to SmartPLS structural equation modeling, bootstrapping, and blindfolding.FindingsThe hypothesized relationships were supported. Perceived knowledge quality significantly influences researchers' satisfaction with ASM site use, and satisfaction affects perceived learning and researchers' loyalty with the ASM site.Research limitations/implicationsIdentification of the relationship between perceived knowledge quality and ASM site success extends the study of ASM sites from description of usage patterns to understanding the effect of content quality on important outcomes of use.Practical implicationsASM sites rely on the quality of knowledge contributed by their members for satisfaction, loyalty, and perceptions of value. The ongoing success of an ASM requires directed attention to quality knowledge provision.Originality/valueThis paper contributes a simplified DeLone & McLean information system success framework for studies of content quality. It also provides fresh insights into ASM site usage through a focus on the role of perceived knowledge quality in forming satisfaction, learning, and loyalty.