ABSTRACT This study examined the carbon (C) concentration of the major tree species (Scots pine, Norway spruce, and Birch) in Sweden based on destructively sampled biomass data. The examination was made using single trees and comprised three components: branches with needles, stemwood with bark, and stump with roots. We developed a weighted C concentration estimator accounting for fresh weights of tree components to infer the C concentration at the population level. Significant differences in C concentration (%) were found among species, with the highest in pine (50.671 ± 0.211), followed by spruce (49.518 ± 0.164) and the lowest in birch (49.347 ± 0.241). Additionally, the C concentration varied among tree components, regardless of tree size, growth rate, and site conditions. At the national level, we applied the estimated species- and component-specific C concentration constants to the measured total biomass of the major tree species from the National Forest Inventory. This extrapolation revealed that the average C concentration of major trees across all forestlands in Sweden was approximately 50.012%. These findings have significant implications for accurate C sequestration reporting in the LULUCF sector.