Typically, in partial discharges (PD) diagnostics tests carried out in a laboratory, harmonic content in a voltage waveform is ignored. However, during the operation of variable-frequency drives (VFD)-fed electric motors, the distorted voltage waveform is produced due to the addition of harmonics components in the sinusoidal voltage waveform. The distortion level increases when the VFD is operated at a lower speed ratio. The additional spectral components in the applied voltage waveform can have a significant impact on the PD behavior in the insulation of the electric motor. Therefore, during online PD monitoring, overlooking the harmonic regime in the applied voltage may lead to a false interpretation of PD data. This article investigates the influence of voltage harmonics distortion produced during the variable-speed operation of VFD on PD characteristics. For this purpose, online PD measurements have been carried out by performing a series of experiments on eight VFD-fed motors under variable operating conditions. The PD severity at different harmonic compositions is experimentally investigated by determining various PD characteristic parameters including PD inception voltage, accumulated apparent charge, average discharge current, discharge power, and quadratic rate. Also, two terms Gaussian models have been mathematically developed using 288 data samples to quantify the variations in PD characteristic parameters against distortion parameters. The article proposes a framework for properly evaluating harmonics impact in PD for electric motors and correctly estimating stator insulation degradation.