The essential oil extracted from Borago officinalis L. aerial parts using hydrodistillation contains several compounds, although it yields low amounts. For the first time, this study investigated the effects of hydrodistillation parameters and optimized conditions to maximize the yield of B. officinalis essential oil while characterizing its chemical composition using gas chromatography-mass spectrometry. A single-factor evaluation of three extraction parameters-extraction time, liquid-to-solid ratio, and soaking time-revealed optimal conditions of 4h, 10:1mL/g, and 2h, respectively. Subsequently, the Box-Behnken response surface method produced a significant polynomial model, determining optimal conditions of an extraction time of 5h, a liquid-to-solid ratio of 11.06:1mL/g, and a soaking time of 2h, 17min, and 34s, resulting in an essential oil yield of 0.42%. A total of 21 compounds were identified in B. officinalis essential oil, predominantly nonacosane (29.15%) and phytol (27.92%).
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