Simultaneous Regulation Research Articles

Network Pharmacology and Experimental Validation of the Anti-Inflammatory Effect of Tingli Dazao Xiefei Decoction in Acute Lung Injury Treatment.

Tingli Dazao Xiefei Decoction (TDXD) is a Traditional Chinese Medicine (TCM) formula used to treat acute lung injury (ALI). However, the precise mechanism of TDXD in treating ALI remains unclear. We investigated the therapeutic mechanism of TDXD against ALI using a complementary approach combining network pharmacology, molecular docking, and in vitro and in vivo experiments. Potential drug targets of TDXD and relevant target genes associated with ALI were retrieved from Chinese medicines and disease genes databases. Bioinformatics technology was employed to screen potential active ingredients and core targets. Validation experiments were conducted using a lipopolysaccharide (LPS)-induced ALI mouse (C57BL/6J) model, LPS-induced inflammatory RAW264.7 cells, and molecular docking between active compounds of TDXD and potential targets. Network pharmacology suggested that the mechanism of TDXD against ALI involved phosphoinositide 3-kinase (PI3K) / protein kinase B (AKT) / phosphatase and tensin homolog (PTEN) and Janus kinase 2 (JAK2) / signal transducer and activator of transcription 3 (STAT3) pathways. Quercetin, β-sitosterol, kaempferol, isorhamnetin, and L-stepholidine were identified as the main active compounds of TDXD that exerted anti-ALI effects. Molecular docking indicated that these compounds exhibited good binding capabilities (≤ -5kcal/mol) to key targets in PI3K/AKT/PTEN and JAK2/STAT3 signaling pathways. In the animal model, TDXD alleviated injuries and inflammatory responses in lung tissues, accompanied by inhibition of expression of tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), STAT3, and Suppressor of Cytokine Signaling 3 (SOCS3) mRNA, and key proteins in PI3K/AKT/PTEN and JAK2/STAT3 pathways (all P values < 0.05). Cell based experiments showed that TDXD dose-dependently inhibited the expression of essential proteins in PI3K/AKT/PTEN and JAK2/STAT3 pathways (P < 0.05). This study revealed that the mechanism of TDXD in ALI treatment might involve simultaneous regulation of PI3K/AKT/PTEN and JAK2/STAT3 pathways.

Thermal ablation with configurable shapes: a comprehensive, automated model for bespoke tumor treatment

BackgroundMalignant tumors routinely present with irregular shapes and complex configurations. The lack of customization to individual tumor shapes and standardization of procedures limits the success and application of thermal ablation.MethodsWe introduced an automated treatment model consisting of (i) trajectory and ablation profile planning, (ii) ablation probe insertion, (iii) dynamic energy delivery (including robotically driven control of the energy source power and location over time, according to a treatment plan bespoke to the tumor shape), and (iv) quantitative ablation margin verification. We used a microwave ablation system and a liver phantom (acrylamide polymer with a thermochromic ink) to mimic coagulation and measure the ablation volume. We estimated the ablation width as a function of power and velocity following a probabilistic model. Four representative shapes of liver tumors < 5 cm were selected from two publicly available databases. The ablated specimens were cut along the ablation probe axis and photographed. The shape of the ablated volume was extracted using a color-based segmentation method.ResultsThe uncertainty (standard deviation) of the ablation width increased with increasing power by ± 0.03 mm (95% credible interval [0.02, 0.043]) per watt increase in power and by ± 0.85 mm (95% credible interval [0, 2.5]) per mm/s increase in velocity. Continuous ablation along a straight-line trajectory resulted in elongated rotationally symmetric ablation shapes. Simultaneous regulation of the power and/or translation velocity allowed to modulate the ablation width at specific locations.ConclusionsThis study offers the proof-of-principle of the dynamic energy delivery system using ablation shapes from clinical cases of malignant liver tumors.Relevance statementThe proposed automated treatment model could favor the customization and standardization of thermal ablation for complex tumor shapes.Key points• Current thermal ablation systems are limited to ellipsoidal or spherical shapes.• Dynamic energy delivery produces elongated rotationally symmetric ablation shapes with varying widths.• For complex tumor shapes, multiple customized ablation shapes could be combined.Graphical

Weak-Water-Coordination Electrolyte to Stabilize Zinc Anode Interface for Aqueous Zinc Ion Batteries.

The performance of zinc-ion batteries is severely hindered by the uncontrolled growth of dendrites and the severe side reactions on the zinc anode interface. To address these challenges, a weak-water-coordination electrolyte is realized in a peptone-ZnSO4 -based electrolyte to simultaneously regulate the solvation structure and the interfacial environment. The peptone molecules have stronger interaction with Zn2+ ions than with water molecules, making them more prone to coordinate with Zn2+ ions and then reducing the active water in the solvated sheath. Meantime, the peptone molecules selectively adsorb on the Zn metal surface, and then are reduced to form a stable solid-electrolyte interface layer that can facilitate uniform and dense Zn deposition to inhabit the dendritic growth. Consequently, the Zn||Zn symmetric cell can exhibit exceptional cycling performance over 3200h at 1.0mAcm-2 /1.0mAhcm-2 in the peptone-ZnSO4 -based electrolyte. Moreover, when coupled with a Na2 V6 O16 ·3H2 O cathode, the cell exhibits a long lifespan of 3000 cycles and maintains a high capacity retention rate of 84.3% at 5.0Ag-1 . This study presents an effective approach for enabling simultaneous regulation of the solvation structure and interfacial environment to design a highly reversible Zn anode.

ECM-Engineered Electrospun Fibers with an Immune Cascade Effect for Inhibiting Tissue Fibrosis.

Tissue regeneration/fibrosis after injury is intricately regulated by the immune cascade reaction and extracellular matrix (ECM). Dysregulated cascade signal could jeopardize tissue homeostasis leading to fibrosis. Bioactive scaffolds mimicking natural ECM microstructure and chemistry could regulate the cascade reaction to achieve tissue regeneration. The current study constructed an ECM-engineered micro/nanofibrous scaffold using self-assembled nanofibrous collagen and decorin (DCN)-loaded microfibers to regulate the immune cascade reaction. The ECM-engineered scaffold promoted anti-inflammatory and pro-regenerative effects, M2 polarization of macrophages, by nanofibrous collagen. The ECM-engineered scaffold could release DCN to inhibit inflammation-associated fibrous angiogenesis. Yet, to prevent excessive M2 activity leading to tissue fibrosis, controlled release of DCN was expected to elicit M1 activity and achieve M1/M2 balance in the repair process. Regulated cascade reaction guided favorable crosstalk between macrophages, endothelial cells and fibroblasts by proximity. Additionally, decorin could also antagonize TGF-β1 via TGF-β/Smad3 pathway to suppress fibrotic activity of fibroblasts. Hence, ECM-engineered scaffolds could exert effective regulation of the immune cascade reaction by microstructure and DCN release and achieve the balance between tissue fibrosis and regeneration. STATEMENT OF SIGNIFICANCE: : With the incidence of up to 74.6%, failed back surgery syndrome (FBSS) has been a lingering issue in spine surgery, which poses a heavy socio-economic burden to society. Epidural fibrosis is believed to be responsible for the onset of FBSS. Current biomaterial-based strategies treating epidural fibrosis mainly rely on physical barriers and unidirectional suppression of inflammation. Regulation of the immune cascade reaction for inhibiting fibrosis has not been widely studied. Based on the simultaneous regulation of M1/M2 polarization and intercellular crosstalk, the ECM-engineered micro/nanofibrous scaffolds constructed in the current study could exert an immune cascade effect to coordinate tissue regeneration and inhibit fibrosis. This finding makes a significant contribution in the development of a treatment for epidural fibrosis and FBSS.

Simultaneous Regulation of Surface Properties and Microstructure of Graphene Oxide Membranes for Enhanced Nanofiltration Performance.

The surface properties and microstructure of graphene oxide (GO)-based membranes are both crucial for enhanced nanofiltration performance. Herein, a GO nanofiltration membrane is fabricated with regulatable surface properties and microstructure via a facile two-step impregnation in KOH and following HCl aqueous solutions. The type and number of oxygen-containing groups in GO membranes change with fewer C-O-C/C-OH and C═O but more COOH groups, and they are readily regulated by alkaline treatment time, which enables enhanced surface hydrophilicity and larger surface ζ potentials. Meanwhile, a few tiny defects are present in the GO sheets, which could increase the number of pores and decrease the length of water nanochannels. Such surface properties and microstructure together determine the excellent nanofiltration performance of the GO membranes with fast and selective water permeation, e.g., ∼99.5% rejection toward CBB G250 and flux of 56.9 ± 1.0 L m-2 h-1. This work provides insights into the design of high-performance two-dimensional laminar membranes.

ASD2023: towards the integrating landscapes of allosteric knowledgebase.

Allosteric regulation, induced by perturbations at an allosteric site topographically distinct from the orthosteric site, is one of the most direct and efficient ways to fine-tune macromolecular function. The Allosteric Database (ASD; accessible online at http://mdl.shsmu.edu.cn/ASD) has been systematically developed since 2009 to provide comprehensive information on allosteric regulation. In recent years, allostery has seen sustained growth and wide-ranging applications in life sciences, from basic research to new therapeutics development, while also elucidating emerging obstacles across allosteric research stages. To overcome these challenges and maintain high-quality data center services, novel features were curated in the ASD2023 update: (i) 66589 potential allosteric sites, covering>80% of the human proteome and constituting the human allosteric pocketome; (ii) 748 allosteric protein-protein interaction (PPI) modulators with clear mechanisms, aiding protein machine studies and PPI-targeted drug discovery; (iii) 'Allosteric Hit-to-Lead,' a pioneering dataset providing panoramic views from 87 well-defined allosteric hits to 6565 leadsand (iv) 456 dualsteric modulators for exploring the simultaneous regulation of allosteric and orthosteric sites. Meanwhile, ASD2023 maintains a significant growth of foundational allosteric data. Based on these efforts, the allosteric knowledgebase is progressively evolving towards an integrated landscape, facilitating advancements in allosteric target identification, mechanistic explorationand drug discovery.

Coordination or contradiction: Evidence from water and air pollution regulation in China

Using a difference-in-differences strategy, we study the effects of simultaneous water and air pollution regulation on firms' emission behaviors and explore the driving forces behind their responses. It is found that in China's eleventh Five-Year Plan, there exists a contradiction between chemical oxygen demand (COD) and sulfur dioxide (SO2) regulation. We divide emissions into output and emission intensity to explore the underlying mechanisms and explain the regulatory interaction effects from perspectives of source prevention and end-of-pipe treatment. Also, we examine heterogeneous effects across firm size, firm age, and manufacturing agglomeration. Overall, this paper reveals whether coordination or contradiction exists among environmental regulation at the firm level, providing implications for policymaking and policy synergy.

Stabilizing precursor solution and controlling crystallization kinetics simultaneously for high-performance perovskite solar cells.

The efficiency of metal halide perovskite solar cells has skyrocketed, however defects created by aging precursor solutions and during crystallization pose a significant barrier to reproducibility and efficiency of solar cells. Herein, we report an additive method using Fluoro-N,N,N″,N″-tetramethylformamidinium hexafluorophosphate (F-(CH3 )4 CN2 PF6 , abbreviated as TFFH) to stabilize precursor solution and improve crysallization dynamics simutaneously for high-performance FAPbI3 (FA = formamidinium)-based perovskite indoor photovoltaics. The F-(CH3 )4 CN2 cations stabilize precursor solution by inhibiting oxidation of I- and reducing newly generated I0 to I- simultaneously. The PF6 - anions interact strongly with the Pb-I framework to passivate undercoordinated Pb2+ . Time-resolved optical diagnostics show a prolonged perovskite crystallization dynamics during which in situ defect passivation is achieved in the precesence of strong FA+ ···TFFH···Pb-I interaction. Simultaneous regulation of precursor solution and crystallizaiton dynamics guarantee larger perovskite grain sizes, better crystal orientation, and less defects of perovskite films as well as more efficient charge extraction in complete solar cells. The optimized solar cells achieve improved reproducibility, better stability and reach effciency of 42.43% at illumination of 1002 lux, which is the highest efficiency among all indoor photovoltaics. It is anticipated that the concurrent stabilization of solutions and regulation of crystallization dynamics will emerge as a prevalent approach for enhancing the reproducibility and efficiency of perovskite. This article is protected by copyright. All rights reserved.

Enhancing catalytic performance for waste plastic upgrading: Simultaneous regulation of pore structure and acid sites in Ga-doped desilicated HZSM-5 catalysts

Though catalytic pyrolysis through HZSM-5 has shown great potential to convert waste plastics, it is urgent to modify the catalyst to improve its catalytic performance due to the low liquid product yields and inferior selectivity to high value-added monocyclic aromatic hydrocarbons (MAHs). During the study, two main modification methods, desilication via alkali treatment and Ga metal doping, were applied on HZSM-5 to reach simultaneous regulation of pore structure and acid site. It showed that selectivity to MAHs rose from 45.37 % to 58.45 % and 59.54 %, respectively, when 0.1 M and 0.4 M NaOH solutions were used for desilication. After Ga metal doping, selectivity to MAHs further increased to 67.43 % and 67.16 %, respectively, at A 0.1 M 5 %Ga/ZSM-5 and A 0.4 M 5 %Ga/ZSM-5, with selectivity to value-added benzene, toluene, ethylbenzene and xylene (BTEX) rising to 50.86 % and 46.45 % correspondingly. After three times of recycling, the catalyst still remained active with selectivity to MAHs staying above 65 %.

Discovery of first novel sigma/HDACi dual-ligands with a potent in vitro antiproliferative activity

Designing and discovering compounds for dual-target inhibitors is challenging to synthesize new, safer, and more efficient drugs than single-target drugs, especially to treat multifactorial diseases such as cancer. The simultaneous regulation of multiple targets might represent an alternative synthetic approach to optimize patient compliance and tolerance, minimizing the risk of target-based drug resistance due to the modulation of a few targets. To this end, we conceived for the first time the design and synthesis of dual-ligands σR/HDACi to evaluate possible employment as innovative candidates to address this complex disease. Among all synthesized compounds screened for several tumoral cell lines, compound 6 (Kiσ1R = 38 ± 3.7; Kiσ2R = 2917 ± 769 and HDACs IC50 = 0.59 µM) is the most promising candidate as an antiproliferative agent with an IC50 of 0.9 µM on the HCT116 cell line and no significant toxicity to normal cells. Studies of molecular docking, which confirmed the affinity over σ1R and a pan-HDACs inhibitory behavior, support a possible balanced affinity and activity between both targets.

A robust optimal distributed control design for simultaneous voltage regulation and current sharing in DC microgrid

AbstractIn this article, a robust optimal distributed controller is designed for achieving average voltage regulation and current sharing in a DC microgrid. Each distributed generation unit (DGU) consists of a DC‐DC buck converter that supplies a local constant current load. The proposed controller only utilises output currents of converters and the possible maximum error values in estimation of series resistance in the RLC filter used in each DGU. The proposed controller is formed such that the possible estimation error is considered as an uncertain parameter. In addition to obtaining two main objectives, average voltage regulation, and current sharing, a robust control problem is solved through an optimal control technique to calculate controller parameters such that the stability of the grid is guaranteed. The stability of the closed loop system with parametric uncertainty is proved through Lyapunov analysis. The closed loop system with the suggested controller is tested by simulations and through different scenarios to reveal the reliable and satisfactory performance of the proposed robust optimal control structure.

A new class of protein sensor links spirochete pleomorphism, persistence, and chemotaxis.

A new class of bacterial protein sensors monitors intracellular levels of S-adenosylmethionine to modulate cell morphology, chemotaxis, and biofilm formation. Simultaneous regulation of these behaviors enables bacterial pathogens to survive within their niche. This sensor, exemplified by Treponema denticola CheWS, is anchored to the chemotaxis array and its sensor domain is located below the chemotaxis rings. This position may allow the sensor to directly interact with the chemotaxis histidine kinase CheA. Collectively, these data establish a critical role of CheWS in pathogenesis and further illustrate the impact of studying non-canonical chemotaxis proteins.

Simultaneous regulation of strength and ductility of laser powder bed fusion manufactured Haynes 230 alloy

Haynes 230 alloy is widely used in aero-engines with high strength, fatigue resistance, oxidation resistance and other properties. However, the powder metallurgy process limits its application due to hot cracks and low strength. In this work, crack inhibition and mechanical properties improvement of the laser powder bed fusion (L-PBF) manufactured Haynes 230 alloy using 25 groups of different laser energy densities and scanning strategies were investigated. Results show that the high density, nearly crack-free, fine microstructure, and excellent strength and ductility were achieved by a laser energy density of 59.4 J/mm3 and rotation angle of 90° for the L-PBF manufactured Haynes 230 alloy. The microstructure was composed of γ matrix, carbides (M6C, M23C6), and La2O3 nanoparticles. Interestingly, many carbides were found to be mainly uniformly distributed at the grain or sub-grain boundaries with the following orientation relation: [011]γ//[011]M23C6. Simultaneously, by adjusting laser energy density and scanning strategy, the liquid film, low-melting eutectic phase, hot stress concentration, and high-angle grain boundaries (HAGBs) were suppressed, and the hot cracks were reduced. In addition, yield strength and ultimate tensile strength enhancement of the L-PBF manufactured Haynes 230 alloy was attributed to the synergistic effect of solid solution strengthening, dislocation strengthening, Orowan strengthening, and grain refinement. Whereas ductility enhancement was the main effect of defect inhibition, grain refinement, and nanoprecipitate. This work has verified the feasibility of crack inhibition and mechanical properties improvement of the L-PBF manufactured Haynes 230 alloy by various laser energy densities and scanning strategies.

Simultaneous regulation on coordination environment and interfacial chemistry via taurine for stabilized Zn metal anode

Aqueous Zn-ion batteries (AZIBs) are the potential options for the next-generation energy storage scenarios due to the cost effectiveness and intrinsic safety. Nevertheless, the industrial application of AZIBs is still impeded by a series of parasitic reactions and dendrites at zinc anodes. In this study, taurine (TAU) is used in electrolyte to simultaneously optimize the coordination condition of the ZnSO4 electrolyte and interfacial chemistry at the anode. TAU can preferentially adsorb with the zinc metal and induce an in situ stable and protective interface on the anode, which would avoid the connection between H2O and the zinc metal and promote the even deposition of Zn2+. The resulting Zn//Zn batteries achieve more than 3000 hours long cyclic lifespan under 1 mA cm−2 and an impressive cumulative capacity at 5 mA cm−2. Moreover, Zn//Cu batteries can realize a reversible plating/stripping process over 2,400 cycles, with a desirable coulombic efficiency of 99.75% (1 mA cm−2). Additionally, the additive endows Zn//NH4V4O10 batteries with more stable cyclic performance and ultrafast rate capability. These capabilities can promote the industrial application of AZIBs.

Obviously Enhanced Fluorescent Signal of Core‐Shell Nanostructures through Simultaneous Regulation of Spectral Overlap and Shell Thickness for Imaging and Photothermal Therapy of Ovarian Cancer Cells

AbstractIn this research, by simultaneously regulating the two major factors affecting the plasmonic enhanced fluorescence (PEF), spectral overlap and the distance between the fluororophores and the noble metal nanoparticles, a significantly enhanced fluorescent signal is achieved. Core‐shell nanostructures composed of aspect ratio (AR) adjustable gold nanorods (GNRs) and various thickness of SiO2 are prepared and the decorated fluorophores are realized optimized PEF. A typical stimuli‐responsive conjugated polymer, polydiacetylene (PDA), and a near‐infrared (NIR) dye Cy5.5 are selected as fluorophores and their fluorescent signal are enhanced 7.26 and 4.41 times, respectively. Based on the optimized optical properties, a multifunctional antibody modified Mab‐Cy5.5‐GNRs@SiO2 is successfully demonstrated the targeting, imaging, and photothermal therapy (PTT) effects on SKOV‐3 ovarian cancer cells.

Simultaneous regulation of biocathodic γ-HCH dechlorination and CH4 production by tailoring the structure and function of biofilms based on quorum sensing

Dechlorination of chlorinated organic pollutants and methanogenesis are attractive biocathode reductions in microbial electrolysis cells (MECs). Quorum sensing (QS) is applied to regulate microbial communications. However, how acyl-homoserine lactones (AHLs)-dependent QS organize the assembly of the biocathode microbial community, and then regulate multiple biocathode reductions remains unclear. By applying N-butanoyl homoserine lactone (C4-HSL), N-hexanoyl homoserine lactone (C6-HSL) and 3-oxo-hexanoyl homoserine lactone (3OC6-HSL) in γ-hexachlorocyclohexane (γ-HCH) contaminated MECs, this study investigated the changes of biofilm microbial structure and function and the mechanisms of AHLs-QS on γ-HCH dechlorination and CH4 production. Exogenous C4-HSL and 3OC6-HSL increased cytochrome c production and enriched dechlorinators, electroactive bacteria but not methanogens to accelerate γ-HCH dechlorination and inhibit CH4 production. C6-HSL facilitated dechlorination and CH4 production by enhancing biofilm electroactivity and increasing membrane transportation. Besides, exogenous C6-HSL restored the electron transfer capacity that was damaged by the concurrent addition of acylase, an endogenous AHL quencher. From the perspective of microbial assembly, this study sheds insights into and provides an efficient strategy to selectively accelerate dechlorination and CH4 production by harnessing microbial structure based on QS systems to meet various environmental demands.

Transcriptomic and genetic approaches reveal that the pipecolate biosynthesis pathway simultaneously regulates tomato fruit ripening and quality

Pipecolic acid (Pip) and N-hydroxypipecolic acid (NHP) have been found to accumulate during the ripening of multiple types of fruits; however, the function and mechanism of pipecolate pathway in fruits remain unclear. Here study was conducted on fruits produced by the model plant tomato, wherein the NHP biosynthesis-related genes, Slald1 and Slfmo1, were mutated. The results showed that the fruits of both the Slald1 and the Slfmo1 mutants exhibited a delayed onset of ripening, decreased fruit size, nutrition and flavor. Exogenous treatment with Pip and NHP promoted fruit ripening and improved fruit quality. Transcriptomic analysis combined with weighted gene co-expression network analysis revealed that the genes involved in the biosynthesis of amino acids, carbon metabolism, photosynthesis, starch and sucrose metabolism, flavonoid biosynthesis, and plant hormone signal transduction were affected by SlFMO1 gene mutation. Transcription factor prediction analysis revealed that the NAC and AP2/ERF-ERF family members are notably involved in the regulation pathway. Overall, our results suggest that the pipecolate biosynthesis pathway is involved in the simultaneous regulation of fruit ripening and quality and indicate that a regulatory mechanism at the transcriptional level exists. However, possible roles of endogenously synthesized Pip and NHP in these processes remain to be determined. The biosynthesis pathway genes SlALD1 and SlFMO1 may be potential breeding targets for promoting fruit ripening and improving fruit quality with concomitant yield increases.

Unraveling the Solvation Structure and Electrolyte Interface through Carbonyl Chemistry for Durable and Dendrite‐Free Zn Anode

AbstractAqueous Zn ion batteries are appealing systems owing to their safety, low cost, and environmental friendliness; however, their practical applicability is impeded by the growth of Zn dendrites and side reactions. Herein, a dual‐functional electrolyte additive, namely acetylacetone (AT) is utilized for the simultaneous regulation of the solventized structure and anode–electrolyte interface (AEI) to achieve a durable, dendrite‐free Zn anode. Theoretical calculations and experimental characterizations reveal that the AT molecule can be adsorbed onto Zn metal surface to reconstruct the AEI and allow for the primordial desolvation of [Zn(H2O)6]2+ at locations away from the surface of the Zn anode during deposition, which is attributed to the strong polarity of the carbonyl functional group. In addition, the two carbonyls of AT can replace two H2O molecules in the primary solventized structure of Zn2+ to reduce the number of active H2O molecules, efficiently suppressing Zn dendrite growth and detrimental reactions. As a proof of concept, a Zn//Cu cell is constructed in ZnSO4 containing 3 vol.% AT electrolyte, delivering stable cycling over 1800 cycles while maintaining a high Coulombic efficiency of 99.74%. This study provides a practical approach for inhibiting dendrite growth and side reactions by harnessing carbonyl chemistry.

Soil ecological stoichiometry synchronously regulates stream nitrogen and phosphorus concentrations and ratios

Whether and how to synchronously regulate stream water nitrogen (N) and phosphorus (P) concentrations and ratios is a major challenge for sustainable aquatic functions. Soil carbon (C):N:P ratios influence soil N and P stocks and biogeochemical processes that elicit subsequent substantial impacts on stream water N and P concentrations and ratios. Therefore, bridging soil and stream water with ecological stoichiometry is one of the most promising technologies for improving stream water quality. Here, we quantified the ecological stoichiometry of soil and stream water relationships across nine catchments. Soil C:P ratio was the main driver of water quality, showing negative correlations with stream water N and P concentrations, and positive correlations with the N:P ratio in P-limited catchments. We revealed that soil C:P ratios higher than 97.8 mol mol−1 are required to achieve the simultaneous regulation of stream water N and P concentrations below the eutrophication threshold and make algal growth P-limited. Furthermore, we found that the relationships between catchment landscape and soil ecological stoichiometry likely provided practical options for regulating soil ecological stoichiometry. Our work highlights that soil ecological stoichiometry can effectively indicate the amount and proportion of soil N and P losses, and can be intervened through rational landscape planning to achieve sustainable aquatic ecosystems in catchments.

Improved stress relaxation stability of a heat-resistant Al-Cu-Mg-Ag alloy with good mechanical properties by a pre-annealing/solution-aging treatment

Good stress relaxation stability enables heat-resistant aluminum alloys to serve well at high temperatures. From the viewpoint of simultaneous regulation of microstructure and precipitates, a deliberate pre-annealing (200–400 ℃) followed by solution-aging (T6) treatment was proposed to improve the stress relaxation stability of an Al-Cu-Mg-Ag heat-resistant aluminum alloy in this work. A peak effect on the stress relaxation stability was found with a pre-annealing temperature of 325 ℃, which is associated with larger-sized grains and finer-sized precipitates of the alloy. This interesting peak phenomenon is attributed to a good balance of recrystallization and precipitation. Moreover, the regulated microstructure surprisingly assisted the 325 ℃ pre-annealing+T6 treated alloy with good tensile properties. The proposed heat treatment method provides a route for developing high-performance heat-resistant aluminum alloys.