Simultaneous Nitrification-denitrification Research Articles

Modified integrated fixed-film activated sludge process: Advanced nitrogen removal for low-C/N domestic wastewater

Actual low-C/N domestic wastewater was treated using the high-concentration powder carrier bio-fluidized bed (HPB) process comparing diatomite and Fe–C as the carriers. The total nitrogen removal efficiencies were increased from 50.08% to 65.40% and 78.58%, respectively. The diatomite HPB process increased the relative abundance of autotrophic N-cycle bacteria to more than twofold and the sludge size. Therefore, the contributions for nitrogen removal by anammox and simultaneous nitrification–denitrification were increased. The Fe–C HPB process improved the nitrogen removal efficiency mainly by increasing the biodegradability and activities of electron transfer system and key enzymes. The key device (hydrocyclone separator) of the HPB process significantly improved the recovery efficiency of the carriers. It also improved the capacity of microbial aggregations for adsorbing pollutants. Furthermore, it reduced the relative abundance of filamentous bacteria. This study demonstrated the feasibility and mechanism of the HPB process for improving the nitrogen removal efficiency for low-C/N wastewater.

Enhancing nitrogen removal of aerobic granular sludge with optimization of dissolved oxygen spatial distribution

This study aims to investigate the impact of the aerator position on the dissolved oxygen spatial distribution and stability of aerobic granular sludge (AGS). Three SBRs with 2.5 mm mesh screen were established under condition of different aerator positions, the aerators of R1, R2 and R3 were set at the bottom, 1/6 and 1/3 height of reactors, respectively. The results revealed that R2 was more favorable to improve the sludge settling behavior and structural stability of AGS, resulting in 83.7 ± 1.4 % of total nitrogen (TN) removal. However, R3 led to an anoxic environment at the bottom of reactor, which prevented the formation of AGS and made a large amount of sludge lost. DO distribution indicated that oxygen content at the bottom of R2 was at the optimum range of 18.9 %–20.1 %, being beneficial to realize the simultaneous nitrification-denitrification. Therefore, the functional microbes of Zoogloea spp. (17.4 %), Paracoccus spp. (12.5 %), Thaurea spp. (13.6 %) and Rhodobacter spp. (10.8 %) were highly enriched in R2. Overall, raising the aerator position to 1/6 height of reactor led to 7.4 % increase of TN removal efficiency and 15.4 % decrease of energy consumption, which favors the application of AGS technology.

Lab‐scale data and microbial community structure suggest shortcut nitrogen removal as the predominant nitrogen removal mechanism in post‐aerobic digestion (PAD)

Implementing an aerobic digestion step after anaerobic digestion, referred to as "post aerobic digestion" (PAD), can remove ammonia without the need for an external carbon source and destroy volatile solids. While this process has been documented at the lab-scale and full-scale, the mechanism for N removal and the corresponding microbial community that carries out this process have not been established. This research gap is important to fill because the nitrogen removal pathway has implications on aeration requirements and carbon demand, that is, short-cut N-removal requires less oxygen and carbon than simultaneous nitrification-denitrification. The aims of this research were to (i) determine if nitrite (NO2 - ) or nitrate (NO3 - ) dominates following ammonia removal and (ii) characterize the microbial community from PAD reactors. Here, lab-scale PAD reactors were seeded with biomass from two different full-scale PAD reactors. The lab-scale reactors were fed with biomass from full-scale reactors and operated in batch mode to quantify nitrogen species concentrations (ammonia, NH4 + , NO2 - , and NO3 - ) over time. Experimental results revealed that NO2 - production rates were several orders of magnitude greater than NO3 - production rates. Indeed, nitrite accumulation rate (NAR) was greater than 90% at most temperatures, confirming that shortcut nitrogen removal was the dominant NH4 + removal mechanism in PAD. Microbial community analysis via 16S rRNA sequencing indicated that ammonia oxidizing bacteria (AOB) were much more abundant than nitrite oxidizing bacteria (NOB). Overall, this study suggests that aeration requirements for post-aerobic digestion should be based on NO2 - shunt and not complete simultaneous nitrification denitrification. PRACTITIONER POINTS: AOB are a key feature of PAD microbial communities NOB are present, but in much lower abundance than AOB High nitrite accumulation ratio suggests shortcut nitrite as the main mechanism for nitrogen removal Nitritation in PAD reactors is sustained at temperatures as high as 40°C No ammonia oxidation occurred at 50°C implying different mechanisms of nitrogen removal including ammonia stripping.

Consistency between the metabolic performance of two aerobic granular sludge systems and the functional groups of bacteria detected by amplicon sequencing.

Two sequential batch reactors (R1 and R2) of aerobic granular sludge (AGS) were inoculated with activated sludge of different origins. The objective was to investigate the granulation and the consistency between the structure of the microbial communities (16S rRNA amplicon sequencing) in each reactor and their metabolic performance (removal of C, N, and P). Both reactors were fed with acetate-based synthetic wastewater, targeting an anaerobic-aerobic cycle reputed to favor the phosphorus- and glycogen-accumulating organisms (PAO and GAO). Stable granulation was achieved in both reactors, where, instead of PAO, the dominant genera were ordinary heterotrophic organisms (OHO) such as Thauera, Paracoccus, and Flavobacterium known for their high capacity of aerobic storage of polyhydroxyalkanoates (PHA). Generally, there was good consistency between the metabolic behavior of each reactor and the bacterial genera detected. Both reactors showed high removals of C and complete nitrification (Nitrosomonas and Nitrospira detected) but a low level of simultaneous nitrification-denitrification (SND) during the aerated phase. The latter causes that nitrates were recycled to the initial phase, in detriment of PAO selection. Meanwhile, the study showed that selecting slow-growing OHOs (with aerobic storage capacity) favors stable granulation, revealing an alternative AGS technology for C and N removal.

Application of low-intensity ultrasound to enhance simultaneous nitrification/iron-based autotrophic denitrification.

Intermittent ultrasound with an intensity of 0.2W/ml was applied during simultaneous nitrification/iron-based autotrophic denitrification to evaluate its impacts on total nitrogen (TN) removal efficiency and microbial characteristics during low carbon/nitrogen ratio (C/N) wastewater treatment. At an optimal dissolved oxygen (DO) concentration of 1.2mg/L, the TN removal rate was 91 ± 4%, and the TN concentration in the effluent decreased by 31% owing to the ultrasound treatment. In addition, the number of iron-compounds that formed in the sludge and wastewater increased by 55% and 37%, respectively. Low-intensity ultrasound caused a substantial increase in ammonia monooxygenase activity. Moreover, when the DO concentration increased to 1.2mg/L, the activities of nitrate reductase and nitrite reductase, both of which are associated with denitrification, were effectively maintained. High-throughput sequencing indicated that low-intensity ultrasound enriched ammonium oxidising bacteria (Nitrosomonas) and suppressed the growth of heterotrophic denitrifying bacteria (Zoogloea and Simplicispira). These changes benefited simultaneous nitrification and autotrophic denitrification. Thus, low-intensity ultrasound promoted the simultaneous nitrification/iron-based autotrophic denitrification process during low C/N wastewater treatment.

Metagenomics reveals a full-scale modified integrated fixed-film activated sludge process: Enhanced nitrogen removal and reduced sludge production

This study provides a side-by-side comparative investigation between the full-scale conventional activated sludge (CAS) and the high-concentration powder carrier bio-fluidized bed (HPB) processes. The results showed that the HPB total nitrogen removal efficiency increased by 10.86 % more than CAS. The anammox pathway increased by 6.92 %, while the simultaneous nitrification-denitrification pathway increased by 4.27 %. Also, the effluent's total nitrogen of the HPB process was stabilized below 10 mg/L, which can withstand the impact of industrial wastewater better. More energy and substance (protein) were consumed to attach to the carriers and resist external selective pressure to produce extracellular polymeric substance rather than sludge production in the HPB process. For a 10,000 m3/d HPB wastewater treatment plant, lowering the total nitrogen and sludge production saved $110,369.64 in annual operating costs.

Deciphering waste bound nitrogen by employing psychrophillic Aporrectodea caliginosa and priming of coprolites by associated heterotrophic nitrifiers under high altitude Himalayas

Himalayan ecosystem is characterized by its fragile climate with rich repositories of biodiversity. Waste collection and disposal are becoming increasingly difficult due to topographical variations. Aporrectodea caligenosa, a versatile psychrophillic soil dweller, is a useful biocatalyst with potent bio-augmented capability for waste treatment at low temperatures. Microcosm experiments were conducted to elucidate the comprehensive nature of biogenic nitrogen transformation to NH4+ and NO3− produced by coupling of earthworm-microbes. Higher biogenic recovery of NH4+-N from coprolites of garden soil (47.73 ± 1.16%) and Himalayan goat manure (86.32 ± 0.92%) with an increment of 14.12 and 47.21% respectively over their respective control (without earthworms) with a linear decline beyond 4th week of incubation was reported. NO3–-N recovery progressively sustained in garden soil and goat manure coprolites during entire incubation with highest 81.81 ± 0.45 and 87.20 ± 1.08 µg-N g−1dry weight recorded in 6th and 5th week of incubation respectively and peak increments as 38.58 and 53.71% relative to respective control (without earthworms). Declined NH4+–N in coprolites at low temperature (15.0 ± 2.0 °C) evidenced increased nitrification rates by taking over the process by abundant nitrifying microbes. Steady de-nitrification with progressive incubation on an average was 16.95 ± 0.46 ng-N g−1 per week and 21.08 ± 0.87 ng-N g−1 per week compared to 14.03 ± 0.58 ng-N g−1 per week and 4.50 ± 0.31 ng-N g−1 per week in respective control treatments. Simultaneous heterotrophic nitrification and aerobic denitrification (SHNAD) was found to be a prominent bioprocess at low temperature that resulted in high and stable total nitrogen and nitrate accumulation from garden soil and goat manure with relative recovery efficiency of 11.12%, 14.97% and 14.20%; 19.34%. A. caligenosa shows promising prospects for mass applicability in biogenic N removal from manure of Himalayan goat.

Investigation of an intermittently-aerated moving bed biofilm reactor in rural wastewater treatment under low dissolved oxygen and C/N condition

An intermittently-aerated moving bed biofilm reactor (MBBR) was proposed for nitrogen and carbon removal from low C/N synthetic rural wastewater. In purposes of low energy consumption and costs, the intermittent aeration modes were changed and the dissolved oxygen was reduced gradually during the operation. The results showed that effluent concentrations of ammonia nitrogen and chemical oxygen demand were lower than 15 and 50 mg/L, respectively, even under microaerobic condition (0.1–1.0 mg/L). Meanwhile, the simultaneous nitrification–denitrification was achieved by intermittent aeration. The activity of functional bacteria was still high and the proportion of autotrophic biomass increased significantly under intermittent micro-aeration mode, which improved the nitrification performance. Aerobic denitrifier Hydrogenophaga, anoxic denitrifier Thiothrix, and heterotrophic nitrifier such as Rhodobacter were enriched in the intermittently micro-aerated MBBR, which will provide an applicable solution for rural wastewater treatment under low C/N and costs.

Rapid start-up and advanced nutrient removal of simultaneous nitrification, endogenous denitrification and phosphorus removal aerobic granular sequence batch reactor for treating low C/N domestic wastewater

The rapid start-up and advanced nutrient removal of simultaneous nitrification, endogenous denitrification, and phosphorus (P) removal aerobic granular sequence batch reactor (SNEDPR-AGSBR) is a challenge in the treatment of low carbon/nitrogen (C/N) domestic sewage. In this study, the feasibility of the SNEDPR-AGSBR process was examined in an exceedingly single-stage anaerobic/aerobic/anoxic sequencing batch reactor for treating low C/N ratio (3.3–5.0) domestic sewage. The initial results showed that accompanied by the rapid formation of the mature aerobic granular sludge based on the selection for slow-growing organisms, the rapid start-up (38 d) of the SNEDPR-AGSBR process was successfully realized. The formed mature aerobic granules had a dense structure with an average diameter of 667.7 μm and SVI30 of 30.0 mL/g. Two conditions for achieving the competitive balance between phosphorus-accumulating organisms/denitrifying phosphorus-accumulating organisms (PAOs/DPAOs) and glycogen accumulating organisms/denitrifying glycogen accumulating organisms (GAOs/DGAOs) were revealed by the long-term operation results. First, the dissolved oxygen (DO) concentration needed to be decreased to 3.0 mg/L in the aerobic phase, and then, the aerobic and anoxic phase hydraulic retention time (HRT) should be increased to 3.0 h. Notably, high removal efficiencies for NH4+−N (100%), total nitrogen (84.3%), and P (91.8%) of the SNEDPR-AGSBR process were stably obtained with a low C/N ratio of 3.9 domestic sewage. Simultaneous nitrification and endogenous denitrification (SNED) efficiency of 61.6% was achieved during a long-term operation of 142 days. Finally, microbial community analysis confirmed that GAOs (Defluviicoccus)/DGAOs (Candidatus_Competibacter) were responsible for the removal N, and PAOs (Acinetobacter, Candidatus_Accumulibacter, Hypomicrobinm)/DPAOs (Pseudomonas and Dechloromonas) ensured P removal.

A divided flow aerobic-anoxic baffled reactor for simultaneous nitrification-denitrification of domestic wastewater

A novel aerobic-anoxic baffled reactor is designed for the effective denitrification of real domestic wastewater without an external carbon source. The flow is divided between two inlets at the beginning of each zone to provide a carbon source for the denitrifying bacteria. The effects of operating parameters such as the ratio of chemical oxygen demand to nitrogen (COD/N), flow division ratio, and hydraulic retention time (HRT) on the nitrogen removal were investigated. The optimum values of COD/N and HRT were estimated using response surface methodology (RSM) coupled with a central composite experimental design. The addition of porous biomass support media considerably improved the denitrification and removal of COD. Furthermore, the aerobic-anoxic system showed high stability against sudden HRT and COD/N ratio changes. The microbial analysis showed that Alcaligenes, Achromobacter, and Bordetella were the dominant denitrifying bacteria in the anoxic zone, whereas other species coexisted in the aerobic zone.

The combination sequence effect on nitrogen removal pathway in hybrid constructed wetlands treating raw sewage from multiple perspectives

The combination sequence of traditional hybrid constructed wetlands (HCWs) affects the removal of nitrogen in raw sewage, but the effect of the combination sequence on nitrogen removal pathway have seldom been reported, especially the specific conditions allowing anammox to occur. Three-stage HCWs, namely vertical flow (VF), horizontal flow (HF) and surface flow (SF) constructed wetlands, were arranged in six different sequences to investigate nitrogen removal efficiencies and microbial removal pathways using metagenomic and stable isotope analyses. Results showed that the combination sequence significantly affected nitrogen removal pathways in HCWs. We found the best removal of total nitrogen (~50%) and ammonium (NH4+-N, ~99%) in HCWs with a VFCW in the 1st stage. Metagenomic results and stable isotope analyses further indicated that simultaneous nitrification and heterotrophic denitrification were the main pathways in unsaturated VFCW, which depended on the energy substance and electron donor supplied by chemical oxygen demand (CODCr) in raw sewage. Nitrifier, anammox bacteria and autotrophic denitrifies prevailed in the subsequent saturated CWs, which tend to nitrogen loss by partial nitrification and anammox in HFCW when fed with NH4+-N wastewater with low CODCr. Providing NH4+-N and oxygen in low CODCr wastewater was the essential step to facilitate anammox process in HFCW. It implied that the problem of poor nitrogen removal due to carbon limitation could be overcome by optimizing conditions in anammox's favor.

Nitrogen Removal Performance of Novel Isolated Bacillus sp. Capable of Simultaneous Heterotrophic Nitrification and Aerobic Denitrification.

The control of nitrogenous pollutants is a key concern in aquaculture production. Bacillus spp. are commonly used as probiotics in aquaculture, but only a few reports have focused on the simultaneous heterotrophic nitrification and aerobic denitrification (SND) capacity of Bacillus sp. strains. In order to improve nitrogen biodegradation efficiency in the aquaculture industry, the SND capacity of Bacillus sp. strains was evaluated using both individual and mixed nitrogen sources and different sources of organic carbon. Twelve Bacillus sp. isolates were screened from aquaculture pond sediments and shrimp guts for nitrogen biodegradation. Six strains exhibited especially efficient inorganic nitrogen removal capacities in media with individual and mixed nitrogen sources. These strains comprise K8, N2, and N5 (B. subtilis), HYS (B. albus), H4 (B. amyloliquefaciens), and S1 (B. velezensis). The strains grew better when the sole nitrogen source was NH4+-N, but degraded nitrogen in the following order: nitrite nitrogen (NO2--N), ammonium nitrogen (NH4+-N), and nitrate nitrogen (NO3--N). There was no associated NO2--N accumulation, regardless of the nitrogen source. The optimal carbon source for nitrogen removal varied based on different nitrogen sources and associated metabolic pathways. The optimal carbon sources for the removal of NO3--N, NO2--N, and NH4+-N were sodium citrate, sodium acetate, and sucrose, respectively. The application of H4 in recirculating aquaculture water further demonstrated that NO2--N and NH4+-N could be effectively removed. This study thus provides valuable technical support for the bioremediation of aquaculture water.

Micronutrients and COD/N ratio as factors influencing granular size and SND in aerobic granular sequencing batch reactors operated at low organic loading

Simultaneous nitrification-denitrification (SND) depends on dissolved oxygen, granule size and COD/N ratio. Slow-growing nitrifying bacteria at low COD/N ratio lead to the formation of small-size granule. Anoxic denitrification is encouraged at higher granule size. In the present study, the effect of different COD/N ratios and addition of micronutrients (Fe, Zn, Mn, Cu, Co, Mo, B) on granule size and SND was studied in a laboratory-scale aerobic SBR at low organic loading (358.4–1075.2 mg COD.(L·day)−1). Synthetic cheese whey wastewater and ammonia was used. Small-size granules (0.28–0.7 mm) were developed at COD/TKN ratio of 8.6–13.7 with micronutrients and long famine phase (83–92% of the cycle). COD/TKN ratio of 13.7 achieved the best removal of inorganic nitrogen Ni (62%). At COD/TKN ratio of 8.6, without micronutrients, the low COD uptake rate caused shortening of the famine phase to 66%. Large-size granules (3.0 mm) with limitation of substrates diffusion showed low Ni removal and dominant slow-growing rhizobia (Bradyrhizobium) as detected by 16S rRNA gene amplicon sequencing. In small granules, without diffusion limitations and higher ammonia exposition, the energetically unfavorable heterotrophic nitrification occurred. Heterotrophic nitrifiers Lysobacter and Dyadobacter were found. Hydrogenophaga was a potential aerobic denitrifier. SND process was probably performed by AOB, heterotrophic nitrifiers, and anoxic and aerobic denitrifiers.

Simultaneous Heterotrophic Nitrification and Aerobic Denitrification of Water after Sludge Dewatering in Two Sequential Moving Bed Biofilm Reactors (MBBR).

Water after sludge dewatering, also known as reject water from anaerobic digestion, is recycled back to the main wastewater treatment inlet in the wastewater treatment plant Porsgrunn, Norway, causing periodic process disturbance due to high ammonium of 568 (±76.7) mg/L and total chemical oxygen demand (tCOD) of 2825 (±526) mg/L. The main aim of this study was the simultaneous treatment of reject water ammonium and COD using two pilot-scale sequential moving bed biofilm reactors (MBBR) implemented in the main wastewater treatment stream. The two pilot MBBRs each had a working volume of 67.4 L. The biofilm carriers used had a protected surface area of 650 m2/m3 with a 60% filling ratio. The results indicate that the combined ammonia removal efficiency (ARE) in both reactors was 65.9%, while the nitrite accumulation rate (NAR) and nitrate production rate (NPR) were 80.2 and 19.8%, respectively. Over 28% of the reject water’s tCOD was removed in both reactors. The heterotrophic nitrification and oxygen tolerant aerobic denitrification were the key biological mechanisms found for the ammonium removal in both reactors. The dominant bacterial family in both reactors was Alcaligenaceae, capable of simultaneous heterotrophic nitrification and denitrification. Moreover, microbial families that were found with equal potential for application of simultaneous heterotrophic nitrification and aerobic denitrification including Cloacamonaceae, Alcaligenaceae, Comamonadaceae, Microbacteriaceae, and Anaerolinaceae.

Organic matter removal in a simultaneous nitrification–denitrification process using fixed-film system

Swine wastewater treatment is a complex challenge, due to the high organic matter (OM) and nitrogen (N) concentrations which require an efficient process. This study focused on evaluating two different support media for OM and N removal from an Upflow Anaerobic Sludge Blanket (UASB) reactor fed with swine wastewater. Maximum specific nitrification (MSNA) and denitrification (MSDA) activity test for both biofilm and suspended biomass were carried out using as supports: polyurethane foam (R1) and polyethylene rings (R2). The results showed that R2 system was more efficiently than R1, reaching OM removal of 77 ± 8% and N of 98 ± 4%, attributed to higher specific denitrifying activity recorded (5.3 ± 0.34 g NO3-N/g TVS∙h). Furthermore, 40 ± 5% of the initial N in the wastewater could have been transformed into molecular nitrogen through SND, of which only 10 ± 1% was volatilized. In this sense, MSDA tests indicated that suspended biomass was responsible for at least 70% of N removal and only 20% can be attributed to biofilm. SND could be confirmed with the analysis of microbial diversity, due to the presence of the genus Pseudomonas dominated the prokaryotic community of the system in 54.4%.

Performance and mechanism of simultaneous nitrification–denitrification and denitrifying phosphorus removal in long-term moving bed biofilm reactor (MBBR)

The long-term moving bed biofilm reactor (MBBR) with carrier-attached biofilm was successfully operated for simultaneous removal of nitrogen, phosphorus, and COD at various C/N ratios. Results indicated that 99.60%, 63.58%, 78.94%, and 59.64% of NH4+-N, NO3–-N, TN, and TP were removed at C/N ratio, hydraulic retention time (HRT), and carrier film amount of 5, 40 h, and 1.2 mg·g−1. Nitrogen balance analysis showed that more than 89% of nitrogen (C/N = 20, 15, 10, 5) was converted to gas products. Extracellular polymeric substances (EPS), electron transport system activity (ETSA), and enzyme activity of biofilm were evaluated. Protein (PN)/polysaccharose (PS) values and ETSA decreased with the decrease of C/N ratios. Metagenomics sequencing further revealed that the prominent phyla for nitrogen and phosphorus removal were identified including Proteobacteria, Acidobacteria, Nitrospirae, and Chloroflexi. Proteobacteriaand Gammaproteobacteria were identified as the dominant denitrifying phosphate accumulating organisms (PAO) at the phylum and class level, respectively.

Full Scale SBR Municipal Wastewater Treatment Facility Utilization of Simultaneous Nitrification/Denitrification Coupled with Traditional Nitrogen Removal to Meet Water Criterion

Simultaneous nitrification denitrification (SND) is a well-established phenomenon in biological nutrient removal activated sludge systems. Study at a municipal wastewater treatment facility sought to determine nitrogen removal effectiveness within a full-scale sequential batch reactor (SBR) system utilizing SND in conjunction with traditional nitrogen removal. In addition to characterizing extent of SND, the research examined the ability of SND to meet state-based effluent water quality standards. At the selected facility, the average SND efficiency during a two-month sampling period was 52.8%, paralleling results from similar SBR municipal wastewater systems. The observed SBR system had removal efficiencies > 99% for the influent to effluent -N concentrations. The SND process also resulted in average NO3-NO2-N concentration that was 82% lower than the theoretical concentration under comparable circumstances. Overall, nitrogen removal for this SBR system was >99% which typified results in other SND systems, but at a higher Total Nitrogen removal rate.

Simultaneous Nitrification and Autotrophic Denitrification in Fluidized Bed Reactor Using Pyrite and Elemental Sulfur as Electron Donor

Simultaneous Nitrification and Autotrophic Denitrification in Fluidized Bed Reactor Using Pyrite and Elemental Sulfur as Electron Donor

Combining continuous flow aerobic granulation using an external selector and carbon-efficient nutrient removal with AvN control in a full-scale simultaneous nitrification-denitrification process

The James R. Dolorio Water Reclamation Facility in Pueblo, Colorado, uses AvN aeration controls to lower aeration energy while promoting carbon-efficient nutrient removal and hydrocyclone-based wasting to achieve SVI improvements and process intensification. The results from the full-scale installation showed that hydrocyclone-based wasting helped improve settling characteristics by reducing the SVI from 200 ± 52 mL/g to 83 ± 22 mL/g within weeks of operation. PAO and nitrifiers were preferentially retained in dense flocs and granules, while lighter heterotrophic and filamentous organisms were preferentially wasted, thus uncoupling the SRT of these two fractions relative to the overall SRT. The SRT was estimated at 14.4 ± 3.4 days for dense aggregates and 7.1 ± 2.3 days for lighter flocs. The use of AvN control with continuous low DO conditions resulted in low DO conditions (< 0.3 mgO2/L) reducing air demand by 50% while providing excellent nitrogen (effluent TIN < 11 mgN/L) and TP removal (effluent TP < 1 mgP/L) at low primary effluent COD/N ratio of 6.0. The presence of comammox was demonstrated through molecular analysis, while ex-situ batch tests revealed the presence of DPAO, which could have attributed to the energy and carbon-efficient biological nutrient removal.

Achieving biological nutrient removal in an old sewage treatment plant through process modifications – A simulation and experimental study

Modifying old sewage treatment plants (STP) to meet revised stringent discharge standards is of great concern. Process modeling can serve as an essential tool for this. This work reports a BioWin©-activated sludge model/anaerobic digestion for a 55 million litres per day STP located in South India. Fifty state variables and eighty process equations were used in the model to achieve biological nutrient removal. The model was calibrated and was validated with plant data. The proposed modification was to incorporate an intermediate virtual anoxic zone to achieve simultaneous nitrification-denitrification and total dissolved phosphorus (TDP as PO4) removal. Several scenarios, namely, different hydraulic residence time (HRT) between 1.5 to 2.5 h, dissolved oxygen (DO) between 2.5 to 4.5 mg/L, and mixed liquor suspended solid (MLSS) between 2500 to 4500 mg/L in the bioreactor, were studied to identify optimum conditions. The optimum DO and MLSS levels were identified as 4 mg/L and 4000 mg/L, respectively. The optimum HRT of 2 h in aeration zone, 1 h in anoxic, and 3 h in reaeration zone was identified. Implementation of these modifications in the STP, with minimal operational interventions and no capital costs, improved its performance as predicted by the model. The total nitrogen and TDP (as PO4) reduced from 20 mg/L to 8 mg/L and 3.5 mg/L to 0.9 mg/L, respectively, and met the revised discharge standards. This intervention gave a cost saving of approximately 5.6 million USD.