Simultaneous Determination Of Rutin Research Articles

Simultaneous determination of Rutin, Hesperidin and Quercetin contents in solid dietary supplements by high performance liquid chromatography

Simultaneous determination of rutin, hesperidin and quercetin contents in several solid dietary supplement samples is possible by HPLC method. The analysis process usedthe Agilent Zorbax Eclipse XDB C18 column (250 x 4.6 mm; 5 µm), a mobile phase composed of acetonitrile - phosphoric acid solution 0.1% with gradient program. The detection was carried out on a DAD detector at 280 nm for hesperidin and at 254 nm for rutin and quercetin. The limit of quantitation of method was at a low level of concentration (0.021g/100g with rutin, 0.047g/100g with hesperidin and 0.0054g/100g with quercetin); the accuracy of the method was within 97.0 - 101.33%; the precision showed 1.11 - 1.92% of relative standard deviation which showed high repeatability of the method. The method was applied to 20 dietary supplements samples, the results indicated a high level for the selectivity and reliability of the method to be applied.

Simultaneous identification of rutin, chlorogenic acid and gallic acid in Moringa oleifera by densitometric high-performance thin-layer chromatography method

An HPTLC (high-performance thin-layer chromatography) method was carried out to develop a feasible, simple and accurate method for the estimation of exact concentration of flavonoids in Moringa oleifera. The simultaneous determination of rutin, chlorogenic acid, and gallic acid extracted from different parts (leaf, stem, flower, and root) of M. oleifera was performed and analysis was carried out on glass-backed plates (10 cm × 20 cm) coated silica gel 60 F254 (0.2 mm thickness, Merck, Germany) under CAMAG TLC Scanner III with UV densitometrically at λmax = 254 nm. The mobile phase of ethyl acetate:acetone:water:formic acid with a ratio of 6:3:2:2 (v/v) was used for these flavonoids. The findings of HPTLC gave sharp, symmetrical and well-resolved peaks at Rf values of 0.51 ± 0.02, 0.72 ± 0.04 and 0.83 ± 0.03 with linearity ranging 100–700 ng/spot (r2 = 0.9989), (r2 = 0.9992), and (r2 = 0.9984) for rutin (0.066 ± 0.003%), chlorogenic acid (0.033 ± 0.005%), and gallic acid (0.031 ± 0.005%), respectively. The developed method can also be used as a platform for estimation of complex bioactive(s) bio-synthesized in medicinal or aromatic plants.

Simultaneous determination of rutin and ascorbic acid in a sequential injection lab-at-valve system

A green, simple, accurate and highly sensitive sequential injection lab-at-valve procedure has been developed for the simultaneous determination of ascorbic acid (Asc) and rutin using 18-molybdo-2-phosphate Wells-Dawson heteropoly anion (18-MPA). The method is based on the dependence of the reaction rate between 18-MPA and reducing agents on the solution pH. Only Asc is capable of interacting with 18-MPA at pH 4.7, while at pH 7.4 the reaction with both Asc and rutin proceeds simultaneously. In order to improve the precision and sensitivity of the analysis, to minimize reagent consumption and to remove the Schlieren effect, the manifold for the sequential injection analysis was supplemented with external reaction chamber, and the reaction mixture was segmented. By the reduction of 18-MPA with reducing agents one- and two-electron heteropoly blues are formed. The fraction of one-electron heteropoly blue increases at low concentrations of the reducer. Measurement of the absorbance at a wavelength corresponding to the isobestic point allows strictly linear calibration graphs to be obtained. The calibration curves were linear in the concentration ranges of 0.3–24mgL−1 and 0.2–14mgL−1 with detection limits of 0.13mgL−1 and 0.09mgL−1 for rutin and Asc, respectively. The determination of rutin was possible in the presence of up to a 20-fold molar excess of Asc. The method was applied to the determination of Asc and rutin in ascorutin tablets with acceptable accuracy and precision (1–2%).

Pharmacokinetics of Mori Folium flavones and alkaloids in normal and diabetic rats

To study the pharmacokinetic effect of Mori Folium flavones and alkaloids in normal and diabetic rats. An UPLC-TQ-MS method was developed for the simultaneous determination of rutin, isoquercitrin, astragalin, kaempferol, quercetin, chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, DNJ and fagomine in plasma of rats. The diabetic rat model was induced through intravenous injection with alloxan and high-fat diet. Samples of plasma of rats were obtained at different time points, after the rats were administrated with Mori Folium flavones and alkaloids. After the deproteinization with acetonitrile, the concentrations of Mori Foliam constituents in rats at different time points were detected by UPLC-TQ-MS method, and pharmacokinetic parameters were calculated by DAS 2.0 software. The results showed that quercetin and kaempferol reached peak at 0.333 h, indicating that Mori Folium flavonoid constituents were absorbed and distributed quickly. At about 4 h after administration, both of them reached the peak concentrations for the second time, suggesting that they stayed in intestine for a long time. DNJ and fagomine in gastrointestinal tract can be quickly absorbed into blood, and the concentration in plasma reached peak after 0.667 h, suggesting that both of them could be rapidly distributed in the systemic circulation of rats. Cryptochlorogenic acid, neochlorogenic acid, quercetin, kaempferol and rutin were found to have a higher Cmax and AUC0-t in normal rats than those in diabetic rats. The t1/2values of cryptochlorogenic acid and neochlorogenic acid were shorter in diabetic rats, while quercetin, kaempferol and rutin had a longer t1/2value in diabetic rats. Chlorogenic acid, astragalin, isoquercitrin, fagomine had a higher Cmax in diabetic rats, and the t1/2values of astragalin and fagomine were longer, which suggested differences in absorption of active ingredients under normal and diabetic conditions.

Simultaneous determination of rutin, isoquercetin, and quercetin flavonoids in Nelumbo nucifera by high-performance liquid chromatography method.

Objective:The present study was investigated to provide a documentary evidence for the determination of rutin, isoquercetin, and quercetin flavonoids from the flora of Nelumbo nucifera by reversed-phase high-performance liquid chromatography (RP-HPLC).Materials and Methods:RP-HPLC analysis was performed by gradient elution with a low-pressure gradient using 0.5% acetic acid: acetonitrile as a mobile phase with a flow rate of 1.0 ml/min. The separation was done at 26°C using a Kinetex XB-C18 column as stationary phase and the detection wavelength at 356 nm. The proposed method was validated as per International Conference on Harmonisation guidelines with respect to specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ).Results:The validated results were within the acceptable limits. In specificity, the retention time of rutin, isoquercetin, and quercetin peak in the sample was matched with the reference standard peak and showed good resolution. An excellent linearity was obtained with correlation coefficient (r) higher than 0.999. In precision, the repeatability and intermediate showed <1.0% of % relative standard deviation of peak area percentage indicating high precision and accurate. The recovery rate for rutin, isoquercetin, and quercetin was between 99.85%–101.37%, 101.90%–103.24%, and 101.74%–106.73%, respectively. The lower LOD and LOQ of rutin, isoquercetin, and quercetin enable the detection and quantitation of these flavonoids in N. nucifera at low concentrations.Conclusion:The developed analytical method is convenient for the determination of flavonoids content in herbal drugs.

Simultaneous determination of rutin and ascorbic acid mixture in their pure forms and combined dosage form

A simple, rapid, sensitive and selective high performance liquid chromatographic (HPLC) method with ultraviolet detection has been developed for simultaneous determination of ascorbic acid and rutin in pure forms and pharmaceutical dosage form. HPLC separation was performed on Phenomenex C18 analytical column with 0.1% v/v acetic acid in water and acetonitrile (75:25, v/v), as mobile phase. The separation was done at ambient temperature with flow rate of 1mL·min−1 in isocratic mode. HPLC measurements were carried out using ultraviolet detection wavelength at 257nm. The average retention times were 2.72 and 7.00min for ascorbic acid and rutin, respectively. The calibration plots were constructed over the concentration range of 5.0–30.0 for ascorbic acid and 10.0–60.0μg·mL−1 for rutin. The limits of detection were 1.06 and 1.89μg·mL−1 and limits of quantification were 3.54 and 6.31μg·mL−1 for ascorbic acid and rutin, respectively. The proposed HPLC-UV method was successfully applied for determination of ascorbic acid in its tablets and for simultaneous determination of the studied drugs in their laboratory prepared mixtures and in pharmaceutical formulation. Statistical comparisons of the results with the reference method show an excellent agreement and indicate no significant difference in respect to accuracy and precision.

Simultaneous Determination of Rutin, Luteolin, Quercetin, and Betulinic Acid in the Extract of Disporopsis pernyi (Hua) Diels by UPLC.

Disporopsis pernyi (Hua) Diels, which belongs to genus Disporopsis, has been widely used for the treatment of abnormal sweating, chronic cough, and so forth. An ultra-performance liquid chromatography (UPLC) analysis was developed for the determination of rutin, luteolin, quercetin, and betulinic acid in Disporopsis pernyi (Hua) Diels roots. UPLC analysis was conducted by using a Shim-pack XR-ODS column with gradient elution with the mobile phase of acetonitrile and water containing 0.1% formic acid and with a flow rate of 0.2 mL/min, detected at 210, 254, and 280 nm. The method was precise, with relative standard deviation < 2.0%. The recoveries for the four components in Disporopsis pernyi (Hua) Diels were between 98.5 and 100.9%. The average contents of rutin, luteolin, quercetin, and betulinic acid in roots were 5.63, 2.51, 3.87, and 2.41 μg/g, respectively. The method was accurate and reproducible and it can provide a quantitative basis for quality control of Disporopsis pernyi (Hua) Diels.

Simultaneous Determination of Rutin and Quercetin at a Graphite Oxide and Ionic Liquid Modified Electrode by Square Wave Voltammetry

Simultaneous Determination of Rutin and Quercetin at a Graphite Oxide and Ionic Liquid Modified Electrode by Square Wave Voltammetry

Simultaneous determination of six flavonoids in Hyperici Japonici Herba by HPCE-DAD

To establish a high performance capillary electrophoresis method with diode array detection (HPCE-DAD) for simultaneous determination of rutin, isoquercitrin, hyperoside, quercitrin, kaempferol and quercetin in Hyperici Japonici Herba. Based on the mode of capillary zone electrophoresis, 40 mmol x L(-1) borax was used as buffer solution (pH 8.62), uncoated fused silica capillary (56 cm x 64.5 cm x 75 microm) was used, separation voltage was 25 kV, detection wavelength was at 206 nm, column temperature was maintained at 25 degrees C, and sample was injected at 50 mbar, 8 s. Six flavonoids showed good linearity (r > 0.9953) in the range of the tested concentration, the average recoveries of the method were between 98.8%-102.9%. The method is simple, accurate and reproducible, and can be used for quality control of Hyperici Japonici Herba.

Separation and simultaneous determination of rutin, puerarin, daidzein, esculin and esculetin in medicinal preparations by non-aqueous capillary

A simple method for the simultaneous determination of five bioactive components (rutin, puerarin, daidzein esculin and esculetin) in traditional medicinal preparations by non-aqueous capillary electrophoresis with UV detection has been developed for the first time. A running buffer composed of 15% acetonitrile, 2.5% acetic acid and 90 mM sodium cholate in methanol was found to be the most suitable for this separation. The limits of detection for five analytes were over the range of 0.050–1.216 μg ml −1. The relative standard deviations (R.S.Ds.) of the migration times and the peak areas of the analytes were in the range of 1.3–2.9% and 2.2–2.7% (intraday), 1.7–1.9% and 2.8–3.6% (interday), respectively. In the tested concentration range, linear relationships (correlation coefficients: 0.9974 for rutin, 0.9976 for puerarin, 0.9981 for daidzein, 0.9972 for esculin and 0.9929 for esculetin) between peak areas and concentrations of the analytes were obtained. This method has been successfully applied to simultaneous determination of the five bioactive components with recoveries over the range of 89.4–107.4%.

Determination of rutin and forsythin in fruit ofForsythia suspensa(Thunb.) Vahl by capillary electrophoresis-electrochemical detection

Capillary electrophoresis-amperometric detection is evaluated for simultaneous determination of rutin and forsythin. The cyclic voltammogram, hydrodynamic voltammogram, effect of pH, buffer concentration and SDS, and percent organic modifier on separation and detection were studied. Conditions were optimized as follows: 1.2 V detection potential; separation at 12 kV; 5 s at 15 kV for sample injection time and sample injection voltage; mobile phase 20 mM boric acid buffer; pH 8.4, containing 40 mM SDS and 10% (v/v) acetontrile. The method gave low detection limit as 0.001 mg mL−1 and 0.0005 mg mL−1 (S/N=3), wide linear range 0.005–0.5 mg mL−1 for rutin and forsythin, respectively. The relative standard deviations of peak current and migration time for 8 consecutive injections of the standard solution containing 0.1 mg mL−1 each compound were 4.78%, 3.63% and 6.40%, 2.95% for rutin and forsythin, respectively. In addition, levels of the two compounds in traditional Chinese herbal drugs were easily determined.

SIMULTANEOUS DETERMINATION OF RUTIN AND BENZOCAINE IN SUPPOSITORIES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

A simple and precise reversed-phase HPLC procedure for the simultaneous determination of rutin and benzocaine in suppositories is proposed. The chromatographic conditions were: Apex ODS column; methanol-10 mM triethylamine aqueous solution pH 3.0 (57 : 43, v/v) as the mobile phase; detection wavelength, 270 nm. Calibration curves were linear at concentration ranges 6.0 μg ml−1–14.0 μg ml−1 and 1.5 μg ml−1– 3.5 μg ml−1 for rutin and benzocaine, respectively. The reproductibility was 1.05% and 0.95% for rutin and benzocaine, respectively.