Abstract
Simultaneous determination of rutin, hesperidin and quercetin contents in several solid dietary supplement samples is possible by HPLC method. The analysis process usedthe Agilent Zorbax Eclipse XDB C18 column (250 x 4.6 mm; 5 µm), a mobile phase composed of acetonitrile - phosphoric acid solution 0.1% with gradient program. The detection was carried out on a DAD detector at 280 nm for hesperidin and at 254 nm for rutin and quercetin. The limit of quantitation of method was at a low level of concentration (0.021g/100g with rutin, 0.047g/100g with hesperidin and 0.0054g/100g with quercetin); the accuracy of the method was within 97.0 - 101.33%; the precision showed 1.11 - 1.92% of relative standard deviation which showed high repeatability of the method. The method was applied to 20 dietary supplements samples, the results indicated a high level for the selectivity and reliability of the method to be applied.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.